scholarly journals Ultra-high-throughput picoliter-droplet microfluidics screening of the industrial cellulase-producing filamentous fungus Trichoderma reesei

2019 ◽  
Vol 46 (11) ◽  
pp. 1603-1610 ◽  
Author(s):  
Ronglin He ◽  
Ruihua Ding ◽  
John A. Heyman ◽  
Dongyuan Zhang ◽  
Ran Tu
Keyword(s):  
Trichoderma Reesei ◽  
High Throughput ◽  
Filamentous Fungus ◽  
Droplet Microfluidics ◽  
Picoliter Droplet

scholarly journals Printed droplet microfluidics for on demand dispensing of picoliter droplets and cells

2017 ◽  
Vol 114 (33) ◽  
pp. 8728-8733 ◽  
Author(s):  
Russell H. Cole ◽  
Shi-Yang Tang ◽  
Christian A. Siltanen ◽  
Payam Shahi ◽  
Jesse Q. Zhang ◽  
...  
Keyword(s):  
Single Cell ◽  
High Throughput ◽  
Droplet Microfluidics ◽  
Multiple Measurement ◽  
The Core ◽  
On Demand ◽  
Cell Specificity ◽  
Core Technology ◽  
Deterministic Control ◽  
Picoliter Droplet

Although the elementary unit of biology is the cell, high-throughput methods for the microscale manipulation of cells and reagents are limited. The existing options either are slow, lack single-cell specificity, or use fluid volumes out of scale with those of cells. Here we present printed droplet microfluidics, a technology to dispense picoliter droplets and cells with deterministic control. The core technology is a fluorescence-activated droplet sorter coupled to a specialized substrate that together act as a picoliter droplet and single-cell printer, enabling high-throughput generation of intricate arrays of droplets, cells, and microparticles. Printed droplet microfluidics provides a programmable and robust technology to construct arrays of defined cell and reagent combinations and to integrate multiple measurement modalities together in a single assay.

scholarly journals Printed Droplet Microfluidics for on demand dispensing of picoliter droplets and cells

2017 ◽  
Author(s):  
Russell H. Cole ◽  
Shi-yang Tang ◽  
Christian A. Siltanen ◽  
Payam Shahi ◽  
Jesse Q. Zhang ◽  
...  
Keyword(s):  
Single Cell ◽  
High Throughput ◽  
Droplet Microfluidics ◽  
Multiple Measurement ◽  
The Core ◽  
On Demand ◽  
Cell Specificity ◽  
Core Technology ◽  
Deterministic Control ◽  
Picoliter Droplet

AbstractAlthough the elementary unit of biology is the cell, high throughput methods for the microscale manipulation of cells and reagents are limited. The existing options are either slow, lack single cell specificity, or utilize fluid volumes out of scale with those of cells. Here, we present Printed Droplet Microfluidics, a technology to dispense picoliter droplets and cells with deterministic control. The core technology is a fluorescence-activated droplet sorter coupled to a specialized substrate that together act as a picoliter droplet and single cell printer, enabling high throughput generation of intricate arrays of droplets, cells, and microparticles. Printed Droplet Microfluidics provides a programmable and robust technology to construct arrays of defined cell and reagent combinations and to integrate multiple measurement modalities together in a single assay.

scholarly journals cAMP activates calcium signalling via phospholipase C to regulate cellulase production in the filamentous fungus Trichoderma reesei

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Yumeng Chen ◽  
Xingjia Fan ◽  
Xinqing Zhao ◽  
Yaling Shen ◽  
Xiangyang Xu ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Phospholipase C ◽  
Trichoderma Reesei ◽  
Filamentous Fungus ◽  
Calcium Signalling ◽  
Signal Transduction Pathway ◽  
Cellulase Production ◽  
Signalling Pathway ◽  
Dependent Manner ◽  
Transduction Pathway

Abstract Background The filamentous fungus Trichoderma reesei is one of the best producers of cellulase and has been widely studied for the production of cellulosic ethanol and bio-based products. We previously reported that Mn2+ and N,N-dimethylformamide (DMF) can stimulate cellulase overexpression via Ca2+ bursts and calcium signalling in T. reesei under cellulase-inducing conditions. To further understand the regulatory networks involved in cellulase overexpression in T. reesei, we characterised the Mn2+/DMF-induced calcium signalling pathway involved in the stimulation of cellulase overexpression. Results We found that Mn2+/DMF stimulation significantly increased the intracellular levels of cAMP in an adenylate cyclase (ACY1)-dependent manner. Deletion of acy1 confirmed that cAMP is crucial for the Mn2+/DMF-stimulated cellulase overexpression in T. reesei. We further revealed that cAMP elevation induces a cytosolic Ca2+ burst, thereby initiating the Ca2+ signal transduction pathway in T. reesei, and that cAMP signalling causes the Ca2+ signalling pathway to regulate cellulase production in T. reesei. Furthermore, using a phospholipase C encoding gene plc-e deletion strain, we showed that the plc-e gene is vital for cellulase overexpression in response to stimulation by both Mn2+ and DMF, and that cAMP induces a Ca2+ burst through PLC-E. Conclusions The findings of this study reveal the presence of a signal transduction pathway in which Mn2+/DMF stimulation produces cAMP. Increase in the levels of cAMP activates the calcium signalling pathway via phospholipase C to regulate cellulase overexpression under cellulase-inducing conditions. These findings provide insights into the molecular mechanism of the cAMP–PLC–calcium signalling pathway underlying cellulase expression in T. reesei and highlight the potential applications of signal transduction in the regulation of gene expression in fungi.

Inertial-ordering-assisted droplet microfluidics for high-throughput single-cell RNA-sequencing

Lab on a Chip ◽  
2018 ◽  
Vol 18 (5) ◽  
pp. 775-784 ◽  
Author(s):  
Hui-Sung Moon ◽  
Kwanghwi Je ◽  
Jae-Woong Min ◽  
Donghyun Park ◽  
Kyung-Yeon Han ◽  
...  
Keyword(s):  
Single Cell ◽  
Rna Sequencing ◽  
High Throughput ◽  
Droplet Microfluidics ◽  
Single Cell Rna Sequencing ◽  
Spiral Microchannel

We developed a modified high-throughput droplet barcoding technique for single-cell Drop-Seq via introduction of hydrodynamic ordering in a spiral microchannel.

Efficient Production of Antibody Fragments by the Filamentous Fungus Trichoderma reesei

1993 ◽  
Vol 11 (5) ◽  
pp. 591-595 ◽  
Author(s):  
Eini Nyyssönen ◽  
Merja Penttilä ◽  
Anu Harkki ◽  
Anu Saloheimo ◽  
Jonathan K. C. Knowles ◽  
...  
Keyword(s):  
Trichoderma Reesei ◽  
Filamentous Fungus ◽  
Antibody Fragments ◽  
Efficient Production

scholarly journals A high-throughput cellulase screening system based on droplet microfluidics

2014 ◽  
Vol 8 (4) ◽  
pp. 041102 ◽  
Author(s):  
Raluca Ostafe ◽  
Radivoje Prodanovic ◽  
W. Lloyd Ung ◽  
David A. Weitz ◽  
Rainer Fischer
Keyword(s):  
High Throughput ◽  
Droplet Microfluidics ◽  
Screening System

Droplet-based microfluidics in biomedical applications

2021 ◽  
Author(s):  
Leyla Amirifar ◽  
Mohsen Besanjideh ◽  
Rohollah Nasiri ◽  
Amir Shamloo ◽  
Fatemeh Nasrollahi ◽  
...  
Keyword(s):  
Drug Delivery ◽  
High Throughput ◽  
Paradigm Shift ◽  
Cell Biology ◽  
Biomedical Applications ◽  
Molecular Detection ◽  
Droplet Microfluidics ◽  
Droplet Generation ◽  
Microfluidic Platforms ◽  
Standard Methods

Abstract Droplet-based microfluidic systems have been employed to manipulate discrete fluid volumes with immiscible phases. Creating the fluid droplets at microscale has led to a paradigm shift in mixing, sorting, encapsulation, sensing, and designing high throughput devices for biomedical applications. Droplet microfluidics has opened many opportunities in microparticle synthesis, molecular detection, diagnostics, drug delivery, and cell biology. In the present review, we first introduce standard methods for droplet generation (i.e., passive and active methods) and discuss the latest examples of emulsification and particle synthesis approaches enabled by microfluidic platforms. Then, the applications of droplet-based microfluidics in different biomedical applications are detailed. Finally, a general overview of the latest trends along with the perspectives and future potentials in the field are provided.

Sign in / Sign up

Export Citation Format

Share Document