Genetic diversity in lime (Citrus aurantifolia Swing.) and lemon (Citrus limon (L.) Burm.) based on quantitative traits in India

2015 ◽  
Vol 90 (3) ◽  
pp. 447-456
Author(s):  
A. K. Dubey ◽  
R. M. Sharma ◽  
O. P. Awasthi ◽  
Manish Srivastav ◽  
Nimisha Sharma
Author(s):  
Bidush Ranjan Swar ◽  
V. Swarnalatha ◽  
M. Rajendar Reddy ◽  
S. Vanisree

Soybean MAGIC lines are highly variable breeding material which utilizes both recent and historic genetic recombination events. Present investigation was carried out to assess the genetic diversity present among 95 soybean MAGIC lines along with six checks for 10 different quantitative traits. All the genotypes were grouped into 16 clusters by performing Tocher’s clustering method using Mahalanobis D2 distance. Cluster I was the largest comprising of 30 genotypes followed by cluster II (23 genotypes), cluster X (15 genotypes) and cluster IX (9 genotypes). The maximum genetic distance (D2) was observed between cluster XI and XV (168.37) followed by cluster III and XV (164.3), cluster X and XV (149.64) as well as between cluster XII and cluster XVI (145.99). The cluster mean for most of the traits were high in cluster I and cluster XVI. Oil content contributed maximum (23.86%) towards total genetic diversity followed by number of pods plant-1 (18.97%), seed yield plant-1 (18.63%), 100 seed weight (11.05%) and number of branches plant-1 (10.16%) traits. The soybean MAGIC lines belong to the cluster XI (6-120) and cluster XV (6-30, 6-31, 6-5) were found to be the most divergent hence can be utilised in the recombination breeding programs to exploit maximum heterosis.


1987 ◽  
Vol 17 (5) ◽  
pp. 402-407 ◽  
Author(s):  
S. A. Merkle ◽  
W. T. Adams

Gametophytes from wind-pollinated seeds of Douglas-fir (Pseudotsugamenziesii (Mirb.) Franco var. menziesii) parent trees (mean 56) in each of 22 breeding zones in southwest Oregon were analyzed electrophoretically for gene frequency patterns at 27 loci. Allozyme variability levels were high, as shown by breeding-zone averages for the percentage of polymorphic loci (71.7%, 0.99 criterion), mean number of alleles per locus (2.46), and expected heterozygosity (0.178). Differences among zones in allele frequency were significant (p < 0.05) for only 2 of the 27 loci surveyed, and analysis of hierarchical population structure showed that less than 1% of genetic diversity was attributable to differences among breeding zones. Genetic distance between zones was small [Formula: see text] and, in general, bore no relation to geographical or environmental distance. The limited allozyme differentiation among zones contrasts strikingly with the environment-related variation in seedling quantitative traits previously reported for southwest Oregon Douglas-fir. Allozymes do not appear to be useful for mapping patterns of adaptive variation or for certifying Douglas-fir seed in this region.


2019 ◽  
Vol 42 ◽  
pp. e43426
Author(s):  
Luana Rainieri Massucato ◽  
Karina Kazue Nakamura ◽  
Paulo Mauricio Ruas ◽  
Douglas Mariani Zefa ◽  
Derly José Henrique da Silva ◽  
...  

The conservation of okra landraces [Abelmoschus esculentus (L.) Moench] in gene banks is essential for the success of their use in breeding programmes. This study evaluated the genetic diversity among okra landraces in Brazil based on morphoagronomic descriptors and AFLP markers. We studied 30 accessions of the vegetable gene bank of the Universidade Federal de Viçosa. To this end, 17 morphoagronomic descriptors and five combinations of AFLP primers were used. Genetic parameters were estimated for the quantitative traits and the accessions were grouped by Ward’s method, using the Gower’s and Jaccard’s distance measures, respectively, for the morphoagronomic and molecular data. Polymorphisms were observed for all qualitative traits, while the quantitative traits were significant by deviance analysis. The genetic parameters confirmed the existence of variability among accessions, and high accuracy and heritability indices were found for the traits related to fruit and plant height. Ward’s grouping showed no relationship between the clusters formed with the morphoagronomic and molecular data and the geographical origin of the accessions. No association between morphoagronomic descriptors and AFLP markers was observed. The lack of correlation suggests that both approaches of characterization are important to understand and differentiate the okra accessions.


2017 ◽  
Vol 8 (6) ◽  
pp. 1397
Author(s):  
Manuel De Jesús Bermúdez Guzmán ◽  
Luis Felipe Guzmán Rodríguez ◽  
Karina De la Paz García Mariscal ◽  
Paola Andrea Palmeros Suárez ◽  
Mario Orozco Santos

  La apomixis es un tipo de reproducción asexual donde la formación de semillas porta embriones genéticamente idénticos al progenitor, constituyendo un obstáculo en programas de mejoramiento genético de muchas especies vegetales, incluyendo cítricos. La identificación de plantas híbridas se realiza mediante caracteres morfológicos, ensayos isoenzimáticos y marcadores moleculares. Estos últimos se han utilizado con mayor frecuencia debido a su precisión, destacando el uso del DNA polimórfico amplificado al azar (RAPD, “Random Amplified Polymorphic DNA”) y Secuencias Simples Repetidas (SSR, “Simple Sequence Repeats”). En limón mexicano (C. aurantifolia) únicamente se han utilizado marcadores RAPD para la identificación de híbridos, por lo que no existen reportes que hagan uso de marcadores SSR para este fin. El objetivo del presente trabajo fue identificar híbridos derivados de la polinización controlada entre C. aurantifolia var. “Colimex” X C. limon var. “Rosenberg” y su recíproca utilizando marcadores moleculares SSR. Durante el año 2014-2016 se colectaron hojas de árboles de limón de aproximadamente 12 meses de edad, que se encuentran establecidos en el Campo Experimental Tecomán del INIFAP. Se evaluaron en total ocho marcadores moleculares SSR sobre los progenitores utilizados en este estudio y fueron seleccionados los oligonucleótidos TAA45 y cAGG09 para la identificación de híbridos en las dos poblaciones progenie. De un total de 40 y 43 individuos F1 procedentes de la cruza bidireccional entre “Colimex” X “Rosenberg”, se lograron identificar 17 y 35 plantas híbridas, respectivamente. Los resultados indican que los marcadores SSR son eficientes y confiables para la identificación de híbridos de limón mexicano.


Author(s):  
Jakkam Mahipal Reddy ◽  
Gabrial M. Lal ◽  
Velugoti Priyanka Reddy ◽  
Subhadra Pattanayak ◽  
V. Rohith Guptha ◽  
...  

A trail was conducted during rabi 2020 to study genetic diversity among 36 favorable genotypes of chickpea (Cicer arietinum L.) with help of Mahalanobis D2 statistics. On the basis of D2 values, 36 genotypes were arranged into 5 clusters. The intra cluster distances were lower than inter-cluster distances, specifying that genotypes comprised within a cluster shows tendency to vary less apart from each other. Out of thirteen characteristics considered, secondary branches per plant, number of pods per plant, seed yield per plant, harvest index and plant height, contributed very much in relation to genetic divergence. Wide range of variability was noticed for quantitative traits. This suggested that the selection based on these characteristics would be valuable in improving the grain yield. Therefore, a direct selection based on seed yield and component traits may be practiced to choose superior genotypes which could be utilized in breeding program for the development of high yielding chickpea genotypes.


2018 ◽  
Vol 10 (2) ◽  
pp. 33-39
Author(s):  
Normalina Arpi ◽  
Rasdiansyah Rasdiansyah ◽  
Heru Prono Widayat ◽  
Ramadhana Fajri Foenna

Limbah pulp merah kopi (Coffea sp.) mengandung kafein, senyawa fenolik dan antioksidan alami seperti antosianin, betakaroten, polifenol, dan vitamin C. Penelitian ini bertujuan untuk mempelajari pengaruh jenis jeruk, konsentrasi jeruk nipis (Citrus aurantifolia) dan lemon (Citrus limon) serta penambahan gula terhadap mutu minuman sari pulp kopi arabika (Coffea arabika L.). Rancangan Acak Kelompok (RAK) faktorial digunakan dalam penelitian ini. Faktor 1 jenis jeruk yaitu (J) yaitu J1 = jeruk nipis dan J2 = lemon. Faktor 2, konsentrasi sari jeruk (S) yaitu S1 = 20%, S2 = 30% dan S3 = 40%, serta faktor 3, konsentrasi gula (K) yaitu K1 = 20% dan K2 = 30% dari berat sari pulp kopi. Proses pengolahan dan pemanasan sari pulp kopi dengan penambahan jeruk nipis atau lemon menjadi minuman sari pulp kopi menyebabkan penurunan kandungan vitamin C dan pH. Perlakuan terbaik dalam pembuatan minuman sari pulp kopi yaitu menggunakan jeruk nipis dengan konsentrasi 20% dan gula 30% yang menghasilkan minuman sari pulp kopi dengan nilai sensori hedonik yang lebih baik, dan aktivitas antioksidan (DPPH) 74,9 %, vitamin C 17,6 mg/100g, gula reduksi 19,4 mg/ml, pH 3,40.


2008 ◽  
Vol 51 (1) ◽  
pp. 94-104 ◽  
Author(s):  
Elizabeth Ann Veasey ◽  
Edson Ferreira da Silva ◽  
Eliana Aparecida Schammass ◽  
Giancarlo Conde Xavier Oliveira ◽  
Akihiko Ando

To characterize the genetic variability among species and populations of South American wild rice, eleven populations of Oryza glumaepatula, seven of O. grandiglumis, four of O. latifolia and one of O. alta, from Brazil and Argentina, were evaluated. A greenhouse experiment was conducted in completely randomized blocks with 23 treatments. Twenty morphoagronomic traits were assessed. Univariate analyses were performed with 16 quantitative traits with the partitioning of populations within species. Significant differences (p<0.001) between species were observed for all the traits as well as among populations within the species. The most variable was O. glumaepatula followed by O. latifolia. Multivariate discriminant canonical and cluster analyses confirmed the separation of the highly diverse O. glumaepatula populations from the tetraploid species, and the high genetic variation among O. latifolia populations. Morphological differences among the three tetraploid species seemed to be enough to ascribe them at least the condition of species in statu nascendi.


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