scholarly journals Genetic Diversity Studies in MAGIC Population of Soybean (Glycine max (L.) Merrill) Based on Mahalanobis D2 Distance

Author(s):  
Bidush Ranjan Swar ◽  
V. Swarnalatha ◽  
M. Rajendar Reddy ◽  
S. Vanisree

Soybean MAGIC lines are highly variable breeding material which utilizes both recent and historic genetic recombination events. Present investigation was carried out to assess the genetic diversity present among 95 soybean MAGIC lines along with six checks for 10 different quantitative traits. All the genotypes were grouped into 16 clusters by performing Tocher’s clustering method using Mahalanobis D2 distance. Cluster I was the largest comprising of 30 genotypes followed by cluster II (23 genotypes), cluster X (15 genotypes) and cluster IX (9 genotypes). The maximum genetic distance (D2) was observed between cluster XI and XV (168.37) followed by cluster III and XV (164.3), cluster X and XV (149.64) as well as between cluster XII and cluster XVI (145.99). The cluster mean for most of the traits were high in cluster I and cluster XVI. Oil content contributed maximum (23.86%) towards total genetic diversity followed by number of pods plant-1 (18.97%), seed yield plant-1 (18.63%), 100 seed weight (11.05%) and number of branches plant-1 (10.16%) traits. The soybean MAGIC lines belong to the cluster XI (6-120) and cluster XV (6-30, 6-31, 6-5) were found to be the most divergent hence can be utilised in the recombination breeding programs to exploit maximum heterosis.

2018 ◽  
Vol 40 (1) ◽  
pp. 39324
Author(s):  
Fabiana Mota da Silva ◽  
Elise De Matos Pereira ◽  
Bruno Henrique Pedroso Val ◽  
Dilermando Perecin ◽  
Antonio Orlando Di Mauro ◽  
...  

The success of breeding programs depends on selection procedures and on the breeding methods adopted for selecting segregating populations. The objective of this study was to evaluate the efficiency of the Bulk method with selection in the F3 generation (BulkF3) compared to that of Bulk method as well as determine the most effective selection strategy in terms of genetic gain. Twenty segregating populations were selected by two methods. The 60 best families of each method were selected according to their average agronomic performance. An augmented block design was used. The following agronomic traits were evaluated: insertion height of first pod, plant height at maturity, number of branches and of pods per plant, 100-seed weight, and grain yield. For comparison of the methods, genetic component estimates, genetic gain and predicted breeding values were calculated using mixed models (REML and BLUP). The results showed the families obtained with the BulkF3 method were more productive, showed suitable plant height, a larger number of branches and pods, and higher 100-seed weight. The BulkF3 method was found to be an effective selection strategy for soybean improvement. 


2019 ◽  
Vol 48 (3) ◽  
pp. 481-488
Author(s):  
Dhiman Chakraborty ◽  
Mukul Kumar ◽  
Lobsang Wangchu ◽  
Siddhartha Singh ◽  
AK Pandey

Twenty eight diverse landraces collected from various parts of North Eastern Region of India along with two checks were evaluated to study the diversity pattern among the genotypes on 17 morphological traits. High magnitude of genotypic coefficients of variation (GCV) and heritability coupled with high genetic gain were observed for average fruit weight, fruit yield per plant, number of fruits per plant, fruit length, number of branches per plant, number of seeds per fruit and 100 seed weight. However, these parameters were moderate for early fruit harvest and total soluble solids (TSS). Thirty genotypes were grouped into 6 clusters which showed intercluster D2 values ranging between 115.81 and 670.08. Grouping of genotypes of same location in different clusters indicated that the geographical diversity may not necessarily be related to genetic diversity. The cumulative contribution (82.28%) of fruit weight, 100-seed weight, number of branches per plant and fruit yield per plant to the total divergence indicate the importance of these traits in choice of parents for hybridization programme in cucumber.


Author(s):  
V. Swarnalatha ◽  
Bidush Ranjan Swar ◽  
M. Rajendar Reddy ◽  
S. Vanisree

Soybean MAGIC lines are recently developed by tailor made source of germplasm that have undergone heavy genetic recombination. The present study was carried out in Kharif 2018 to Rabi 2019 - 20 to assess its extent of genetic diversity at molecular level in 95 soybean MAGIC lines along with six checks by using 30 SSR markers from which 27 were found polymorphic. A total of 106 alleles were generated with an average of 3.53 and a range of two to six alleles per loci. The value of observed heterozygosity was varying from 0.00 to 0.099 indicating a higher frequency of homozygotes among the accessions. Maximum PIC was 0.955 for Staga001 followed by 0.948 (Satt168, Satt453, Satt534), 0.947(Satt565) and 0.945(Satt371). The range of Jaccard’s similarity coefficient was varying from 0.089 to 1, most of the values were between 0.2 to 0.3 with an average of 0.3 indicating considerable diversity exists among the genotypes. A total of six main clusters were formed by UPGMA clustering method. Cluster II was the largest comprising 48 genotypes which were grouped into four sub-clusters. Clustering based on SSR markers revealed a very precise grouping of the genotypes based on their relatedness than their phenotypic data alone. Hence it can be successfully deployed for selecting desirable genotypes which can be utilized in future breeding programs for exploiting heterosis or in the introgression of genes for biotic and abiotic stress tolerance in soybean crop.


2019 ◽  
Vol 1 (3) ◽  
pp. 201-207
Author(s):  
Thuy Hang Thi Vu ◽  
Hoa Dinh Vu

Genetic variability and heritability of quantitative traits, particularly of yield contributing traits, are of great importance in understanding phenotypic variation and the heritable portion of the variation when making selection choices. In the present study, the heritability for days to 50% flowering, days to maturity, plant height at maturity, total number of pods per plant, number-filled pods per plant, 100-seed weight, and grain yield per plant were estimated by variance components from variance analysis of parents and F2 and F2:3 progeny families derived from a single cross. Heritability estimates were high for days to 50% flowering and 100-seed weight, moderate for the number of pods per plant and number of filled pods per plant, and low for seed weight per plant. These results suggested that in the early segregating generations, direct selection for seed size, plant height, and a number of pods per plant might be more efficient than the direct selection for seed yield.


2017 ◽  
Vol 1 (01) ◽  
pp. 46-51
Author(s):  
OUMER SHERIFF ◽  
KEFYALEW ALEMAYEHU

Sheriff O, Alemayehu K. 2017. Review: Genetic diversity studies using microsatellite markers and their contribution in supporting sustainable sheep breeding programs. Asian J Agric 1: 46-51. Microsatellites have been widely accepted and employed as useful molecular markers for measuring genetic diversity and divergence within and among populations. The various parameters developed so far to measure genetic diversity within and among populations are observed and expected heterozygosities (Ho and He), the mean number of alleles per locus (MNA),polymorphic information content (PIC), genetic distance and phylogenetic or tree building approach.The objective of thisreview was therefore to quantifythe genetic diversity studies of domestic sheep populations using microsatellite markersand their contribution in supporting sustainable sheep breeding programs. From the review, it is possible to see that there was high within population genetic variations in all the studied sheep populations, poor level of population differentiations and high levels of inbreeding. On the other hand, low estimates of hetrozygosities and mean number of alleles and employing only few and weak markers were observed in some of the studies. The gaps observed in the previous genetic diversity studies of the sheep populations may demand further works to reveal more information on the population structures andto start appropriate and sustainable breeding programs.


Agriculture ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 100
Author(s):  
Tae-Young Hwang ◽  
Byoung-Deok Park ◽  
Jwakyung Sung ◽  
Hong-Sig Kim

The interest in phytochemicals from crops has grown in recent years, with soybean, as a functional crop, having great potential for providing a high level of nutrition. Therefore, the aim of this study was to evaluate phytochemicals from Korean soybean varieties, and to provide the basic information necessary for targeted breeding of soybean. The seeds of 172 soybean varieties were collected from various domestic institutes, and their phytochemicals were analyzed and compared based on agronomic characteristics (color, size, usage, etc.) using cluster analysis. We found that the soybean varieties contained relatively higher levels of the following phytochemicals: lutein (3 var., >7.0 μg g−1 seed), tocopherols (26 var., >300 μg g−1 seed), and phytosterols (33 var., >1000 μg g−1 seed). Overall, phytochemical levels were higher in varieties released since 2000 and with small-sized seeds (<12 g, 100-seed weight). The distribution of phytochemicals varied according to the usage (cooking, sauce, sprouts, etc.), even though the highest levels of each phytochemical were mainly observed in sprouts. The cluster analysis using three main components classified the varieties into five groups. Of them, group III showed relatively higher tocopherols, group IV had relatively higher lutein, and group I had relatively higher phytosterols. Jonam and Manpoong (group I) were observed in varieties high in phytochemicals. Overall, some Korean soybean varieties were found to have relatively higher levels of phytochemicals compared to those reported in previous studies of other origins. In conclusion, our findings suggest that some varieties might provide suitable breeding material for target-centered improvement of soybean varieties, and a comparison between different origins is necessary for providing better knowledge for soybean breeding.


2008 ◽  
Vol 90 (6) ◽  
pp. 481-491 ◽  
Author(s):  
YINGPENG HAN ◽  
WEILI TENG ◽  
DESHENG SUN ◽  
YUPING DU ◽  
LIJUAN QIU ◽  
...  

SummaryThe accumulation of seed mass in soybean is affected by both genotype and environment. The aim of the present study was to measure additive, epistatic and quantitative trait locus (QTL)×environment (QE) interaction effects of QTLs on the development of 100-seed weight in a population of 143 F5 derived recombinant inbred lines (RILs) developed from the cross between the soybean cultivars ‘Charleston’ and ‘Dong Nong 594’. Broad-sense heritability of 100-seed weight from 30 days (30D) to 80D stages was 0·58, 0·52, 0·62, 0·60, 0·66 and 0·57, respectively. A total of 17 QTLs with conditional additive (a) effect and/or conditional additive×environment interaction (ae) effect at specific stages were identified in ten linkage groups by conditional mapping. Of them, only 4 QTLs had significant a effect or ae effect at different stages of seed development. Among QTLs with significant a effect, five acted positively and six acted negatively on seed development. A total of 35 epistatic pairwise QTLs of 100-seed weight were identified by conditional mapping at different developmental stages. Five pairs of QTL showed the additive×additive epistatic (aa) effect and 16 QTLs showed the aa×environment interaction (aae) effect at the different developmental stages. QTLs with aa effect as well with their environmental interaction effect appeared to vary at different developmental stages. Overall, the results indicated that 100-seed weight in soybean is under developmental, genetic and environmental control.


2012 ◽  
Vol 38 (No. 2) ◽  
pp. 69-74 ◽  
Author(s):  
M. Baránek ◽  
M. Kadlec ◽  
J. Raddová ◽  
M. Vachůn ◽  
M. Pidra

The random amplified polymorphic DNA (RAPD) technique was used to evaluate both genetic diversity among 19&nbsp;soybean accessions included in the Czech National Collection of Soybean Genotypes and their potential as a new source of genetic variations for soybean breeding programs. Only 22 of all the 40 random primers used in RAPD reactions showed polymorphism acceptable for an effective characterisation of these accessions. Altogether 122 highly reproducible RAPD fragments were generated, 55 of them were polymorphic (46%). However, because of the previously observed low degree of RAPD polymorphism in the case of Glycine max, fragments with low level of informativeness were evaluated, too. Presented results enable the selection of genetically distinct individuals. Such information may be useful to breeders willing to use genetically diverse introductions in soybean improvement process.&nbsp;


2016 ◽  
Vol 15 (3) ◽  
pp. 283-285 ◽  
Author(s):  
Pawan L. Kulwal ◽  
Laxman B. Mhase

Chickpea (Cicer arietinum L.) is an important food legume crop grown mostly in Asia and Africa as well as in other parts of the world, and serves as an important source of protein in the diets of vegetarian people. Lot of variation in grain quality traits has been observed in cultivated chickpea in both desi and Kabuli types. It has often been observed that seeds with smaller size have more protein when compared with those with larger size. The joint dependence of these two traits on the same primary characteristics results in this negative relationship. This negative correlation coupled with breeding efforts aimed at increasing grain yield has hampered the progress of improving these two traits simultaneously. Therefore, a germplasm exhibiting wider variability for these quantitative traits is an important resource for the identification of novel alleles. Herein, we report the wide variation observed for five important quantitative traits including days to flowering, days to maturity, plant height, 100-seed weight and protein content, and also report the significant positive correlation observed between 100-seed weight and protein content in a diverse collection of chickpea germplasm comprising both desi and Kabuli types.


Author(s):  
Renuka Shivwanshi ◽  
Anita Babbar

A total of 434 germplasm lines of chickpea were assessed for 13 quantitative traits during rabi 2015-16 at JNKVV, Jabalpur. Genotypes were grouped into fourteen clusters. Clusters II and cluster XIV and cluster XII and cluster XIV, had maximum inter cluster distance. The characters viz.,effective pods per plant followed by biological yield per plant, plant height and 100-seed weight were main contribution to total divergence. Cluster XIV and cluster XIII had highest mean values for maximum number of characters. Based on the result IC 83812, EC 198729, EC 490027, IC 53245, IC 83827and IC 83889 are recommended for hybridization, as these genotypes showed good per se performance for seed yield and belong to the highly diverse clusters.


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