Pan-genome analysis of Paenibacillus polymyxa strains reveals the mechanism of plant growth promotion and biocontrol

2020 ◽  
Vol 113 (11) ◽  
pp. 1539-1558
Author(s):  
Liangliang Zhou ◽  
Ting Zhang ◽  
Shan Tang ◽  
Xueqin Fu ◽  
Shuijing Yu
Keyword(s):  
Plant Growth ◽  
Plant Growth Promotion ◽  
Genome Analysis ◽  
Growth Promotion ◽  
Paenibacillus Polymyxa ◽  
Pan Genome

scholarly journals Whole Genome Analysis of Sugarcane Root-Associated Endophyte Pseudomonas aeruginosa B18—A Plant Growth-Promoting Bacterium With Antagonistic Potential Against Sporisorium scitamineum

2021 ◽  
Vol 12 ◽  
Author(s):  
Pratiksha Singh ◽  
Rajesh Kumar Singh ◽  
Dao-Jun Guo ◽  
Anjney Sharma ◽  
Ram Nageena Singh ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Plant Growth ◽  
Plant Growth Promotion ◽  
Genome Analysis ◽  
Growth Promotion ◽  
Rrna Genes ◽  
Whole Genome ◽  
Whole Genome Analysis ◽  
Circular Chromosome ◽  
Sporisorium Scitamineum

Sugarcane smut is a significant fungal disease that causes a major loss in sugar yield and quality. In this study, we isolated an endophytic strain B18 from a sugarcane root, which showed plant growth-promotion, hydrolytic enzyme production, antifungal activity against sugarcane pathogens (Sporisorium scitamineum, Ceratocystis paradoxa, Fusarium verticillioides), and the presence of nifH, acdS, and antibiotic genes (hcn, prn, and phCA) under in vitro conditions. BIOLOG(R) phenotypic profiling of B18 established its ability to use various carbon and nitrogen sources and tolerate a range of pH and osmotic and temperature stresses. Whole-genome analysis of B18, identified as Pseudomonas aeruginosa, showed that it consists of a single circular chromosome of 6,490,014 bp with 66.33% GC content. Genome annotation has identified 5,919 protein-coding genes, and 65 tRNA, and 12 rRNA genes. The P. aeruginosa B18 genome encodes genes related to ethylene, nitrogen (nifU, norBCDERQ, gltBDPS, and aatJMPQ), and phosphate (pstABCS and phoBDHRU) metabolism and produce indole-3-acetic acid and siderophores. This also includes genes encoding hydrolases and oxidoreductases, those associated with biocontrol mechanisms (hcnABC, phzA_B, phzDEFGMS, and pchA), colonization (minCDE and lysC), and biofilm formation (efp, hfq, flgBCDEFGHI, and motAB), and those associated with metabolism of secondary metabolites. Collectively, these results suggest a role for P. aeruginosa B18 in plant growth enhancement and biocontrol mechanisms. The P. aeruginosa B18 strain was found to be an efficient colonizer in sugarcane; it can improve growth through modulation of plant hormone production and enhanced host-plant resistance to smut pathogen S. scitamineum in a smut-susceptible sugarcane variety (Yacheng71-374). These biocontrol and plant growth promotion properties of P. aeruginosa B18 area are discussed in this report.

Plant growth promotion and suppression of bacterial leaf blight in rice by Paenibacillus polymyxa Sx3

2019 ◽  
Vol 68 (5) ◽  
pp. 423-429 ◽  
Author(s):  
Y. Abdallah ◽  
M. Yang ◽  
M. Zhang ◽  
Md. M.I. Masum ◽  
S.O. Ogunyemi ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Promotion ◽  
Growth Promotion ◽  
Paenibacillus Polymyxa ◽  
Leaf Blight ◽  
Bacterial Leaf Blight

Comparison of Paenibacillus polymyxa wild-type and Nif− mutant in colonization, plant-growth promotion and nitrogen fixation contribution

2021 ◽  
Author(s):  
Shuang Liu ◽  
Qin Li ◽  
Yongbin Li ◽  
Tianyi Hao ◽  
Haowei Zhang ◽  
...  
Keyword(s):  
Nitrogen Fixation ◽  
Plant Growth ◽  
Isotope Dilution ◽  
Plant Growth Promotion ◽  
Growth Promotion ◽  
Paenibacillus Polymyxa ◽  
Confocal Laser ◽  
Wild Type ◽  
Greenhouse Experiments ◽  
Dilution Experiments

Abstract Aims This study aimed to compare the effect on colonization, plant-growth promotion and nitrogen fixation contribution by inoculation with Paenibacillus polymyxa wild-type and Nif−mutant. Methods Paenibacillus polymyxa wild-type and Nif− mutant was labeled with GFP and then the GFP-labeled bacteria were used to inoculate cucumber. The colonization patterns of P. polymyxa WLY78 in these plants were observed under the confocal laser scanning microscope. The effects of plant-growth promotion were investigated by greenhouse experiments. The nitrogen fixation contribution was estimated by 15N isotope dilution experiments. Results Observation by laser confocal microscopy revealed that both P. polymyxa WLY78 and ΔnifB-V mutant can effectively colonize cucumber root, stem and leaf tissues. Greenhouse experiments showed that inoculation with P. polymyxa WLY78 can significantly enhance the lengths and fresh wights of cucumber roots and shoots, but inoculation with ΔnifB-V mutant can not. 15N isotope dilution experiments showed that cucumber plants derive 25.93% nitrogen from nitrogen fixation performed by P. polymyxa WLY78, but the ΔnifB-V mutant nearly can not provide nitrogen for plant. Conclusions This present study demonstrates that nitrogen fixation plays an import role in promoting plant growth.

Plant growth promotion and nitrogen fixation in canola (Brassica napus) by an endophytic strain of Paenibacillus polymyxa and its GFP-tagged derivative in a long-term study

Botany ◽  
2016 ◽  
Vol 94 (12) ◽  
pp. 1209-1217 ◽  
Author(s):  
Kiran Preet Padda ◽  
Akshit Puri ◽  
Chris P. Chanway
Keyword(s):  
Brassica Napus ◽  
Plant Growth ◽  
Plant Growth Promotion ◽  
Growth Promotion ◽  
Paenibacillus Polymyxa ◽  
N Fixation ◽  
Brassica Napus L ◽  
Long Term Study ◽  
Seedling Biomass ◽  
Seedling Length

Paenibacillus polymyxa P2b-2R is an endophytic strain capable of fixing nitrogen (N) and promoting growth in a broad range of hosts. In this study, the ability of Paenibacillus polymyxa P2b-2R to fix N and promote the growth of canola (Brassica napus L.) was determined in a 3-month-long trial. We also compared growth promoting and N-fixing capability of wild-type P2b-2R and its GFP-tagged counterpart. Canola seedlings were inoculated with strain P2b-2Rgfp or P2b-2R. Non-inoculated seedlings were used as controls. Canola seedlings were grown for 3 months before evaluating plant growth promotion (length, biomass, and pod mass) and N fixation (using foliar 15N dilution assay). P2b-2Rgfp inoculation significantly promoted canola growth by increasing seedling length by 2-fold, biomass by 2.5-fold, and fixing 35% of its foliar N, respectively. P2b-2R inoculation also increased seedling length by 70%, biomass by 2-fold, and fixed 27% of N. Seedling biomass and length, and the amount of N fixed by P2b-2Rgfp-treated seedlings was significantly greater than the P2b-2R-treated seedlings. P2b-2R or P2b-2Rgfp inoculation also lead to an increase of pod mass (more than 50%), indicating that these strains might play a role in enhancing the yield of canola by acting as biofertilizer.

Genome Analysis, Ecology, and Plant Growth Promotion of the Endophyte Burkholderia phytofirmans Strain PsJN

2013 ◽  
pp. 865-874 ◽  
Author(s):  
Birgit Mitter ◽  
Alexandra Petric ◽  
Patrick SG Chain ◽  
Friederike Trognitz ◽  
Jerzy Nowak ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Promotion ◽  
Genome Analysis ◽  
Growth Promotion ◽  
Burkholderia Phytofirmans Strain Psjn

scholarly journals Genome Mining and Evaluation of the Biocontrol Potential of Pseudomonas fluorescens BRZ63, a New Endophyte of Oilseed Rape (Brassica napus L.) against Fungal Pathogens

2020 ◽  
Vol 21 (22) ◽  
pp. 8740
Author(s):  
Daria Chlebek ◽  
Artur Pinski ◽  
Joanna Żur ◽  
Justyna Michalska ◽  
Katarzyna Hupert-Kocurek
Keyword(s):  
Brassica Napus ◽  
Plant Growth ◽  
Pseudomonas Fluorescens ◽  
Oilseed Rape ◽  
Plant Growth Promotion ◽  
Fungal Pathogens ◽  
Growth Promotion ◽  
Genome Mining ◽  
Plant Colonization ◽  
Brassica Napus L

Endophytic bacteria hold tremendous potential for use as biocontrol agents. Our study aimed to investigate the biocontrol activity of Pseudomonas fluorescens BRZ63, a new endophyte of oilseed rape (Brassica napus L.) against Rhizoctonia solani W70, Colletotrichum dematium K, Sclerotinia sclerotiorum K2291, and Fusarium avenaceum. In addition, features crucial for biocontrol, plant growth promotion, and colonization were assessed and linked with the genome sequences. The in vitro tests showed that BRZ63 significantly inhibited the mycelium growth of all tested pathogens and stimulated germination and growth of oilseed rape seedlings treated with fungal pathogens. The BRZ63 strain can benefit plants by producing biosurfactants, siderophores, indole-3-acetic acid (IAA), 1-aminocyclopropane-1-carboxylate (ACC) deaminase, and ammonia as well as phosphate solubilization. The abilities of exopolysaccharide production, autoaggregation, and biofilm formation additionally underline its potential to plant colonization and hence biocontrol. The effective colonization properties of the BRZ63 strain were confirmed by microscopy observations of EGFP-expressing cells colonizing the root surface and epidermal cells of Arabidopsis thaliana Col-0. Genome mining identified many genes related to the biocontrol process, such as transporters, siderophores, and other secondary metabolites. All analyses revealed that the BRZ63 strain is an excellent endophytic candidate for biocontrol of various plant pathogens and plant growth promotion.

scholarly journals Streptomyces consortia-mediated plant defense against Fusarium wilt and plant growth-promotion in chickpea

2021 ◽  
pp. 104961
Author(s):  
Sravani Ankati ◽  
Vadlamudi Srinivas ◽  
Sambangi Pratyusha ◽  
Subramaniam Gopalakrishnan
Keyword(s):  
Plant Growth ◽  
Plant Defense ◽  
Fusarium Wilt ◽  
Plant Growth Promotion ◽  
Growth Promotion
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