Structure of Rat Testicles after Intravenous Injection of Nanosized Magnetite Particles

The optical properties of colloidal solutions of nanosized magnetite particles in kerosene were studied by optical methods (birefringence and light scattering). The data on the birefringence kinetics in nonstationary magnetic fields is used to determine the size distribution of magnetite particles and aggregates. It is shown that the particle size distribution essentially depends on the type of magnetic moments of the particles and aggregates. Static and dynamic light scattering experiments confirm the conclusion about the existence of a significant fraction of nanoparticles in the form of aggregates with sizes of several tens of nanometers.

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The Local Sanarelli-Shwartzman Phenomenon in Rats Induced by Human Leukaemic Cells

Thrombosis and Haemostasis ◽

10.1055/s-0038-1647777 ◽

1973 ◽

Vol 29 (02) ◽

pp. 353-362

Author(s):

J Lisiewicz ◽

A Pituch ◽

J. A Litwin

Keyword(s):

Chronic Lymphocytic Leukaemia ◽

Intravenous Injection ◽

Peripheral Blood ◽

Lymphocytic Leukaemia ◽

Acute Myeloblastic Leukaemia ◽

Demarcation Line ◽

E Coli ◽

Leukaemic Cells ◽

Shwartzman Phenomenon

SummaryThe local Sanarelli-Shwartzman phenomenon (SSP-L) in the skin of 30 rats was induced by an intr a cutaneous sensitizing injection of leukaemic leucocytes isolated from the peripheral blood of patients with chronic lymphocytic leukaemia (CLL), acute myeloblastic leukaemia (AL) and chronic granulocytic leukaemia (CGL) and challenged by an intravenous injection of 100(μ of E. coli endotoxin. SSP-L was observed in 7 rats after injection of CLL lymphocytes and in 6 and 2 rats after AL myeloblasts and the CGL granulocytes, respectively. The lesions in the skin after AL myeloblasts appeared in a shorter time and were of longer duration compared with those observed after CLL lymphocytes and CGL granulocytes. Histologically, the lesions consisted of areas of destruction in the superficial layers of the skin ; the demarcation line showed the presence of neutrophils, macrophages and erythrocytes. Haemorrhages and fibrin deposits near the demarcation line were larger after injection of CLL lymphocytes and AL myeloblasts than after CGL granulocytes. The possible role of leucocyte procoagulative substances in the differences observed have been discussed.

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Ultrastructural Changes of the Tissue Thromboplastin after Intravenous Injection in Rabbits

Thrombosis and Haemostasis ◽

10.1055/s-0038-1646670 ◽

1978 ◽

Vol 39 (01) ◽

pp. 201-209 ◽

Cited By ~ 1

Author(s):

Hiroshi Hasegawa ◽

Hiroshi Nagata ◽

Makoto Murao

Keyword(s):

Intravenous Injection ◽

Vascular Endothelium ◽

Model Experiment ◽

Venous Return ◽

Ultrastructural Changes ◽

Membrane Structures ◽

Tissue Thromboplastin ◽

Short Time ◽

Sheet Membrane

SummaryAttempts were made to demonstrate ultrastructural changes of the tissue thromboplastin after intravenous injection, as a model experiment on the pulmonary microthrombi formation induced by the tissue thromboplastin circulating from venous return.Concentrically arranged membrane structures of the injected thromboplastin disappeared in extremely short time after the injection of the thromboplastin in rabbits. The long sheet membrane of the injected thromboplastin was frequently seen as adhered to the vascular endothelium or to the surface of blood corpuscles. Furthermore, fibrin fibres were formed in contact with the long sheet membrane of the thromboplastin. Membrane structures were not found anywhere in the control rabbits.

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Turnover of Human Extrinsic (Tissue-Type) Plasminogen Activator in Rabbits

Thrombosis and Haemostasis ◽

10.1055/s-0038-1653442 ◽

1981 ◽

Vol 46 (03) ◽

pp. 658-661 ◽

Cited By ~ 112

Author(s):

C Korninger ◽

J M Stassen ◽

D Collen

Keyword(s):

Plasminogen Activator ◽

Intravenous Injection ◽

Active Site ◽

Half Life ◽

Degradation Products ◽

Gel Filtration ◽

Tissue Type ◽

Organ Distribution ◽

Small Rise

SummaryThe turnover of highly purified human extrinsic plasminogen activator (EPA) (one- and two-chain form) was studied in rabbits. Following intravenous injection, EPA-activity declined rapidly. The disappearance rate of EPA from the plasma could adequately be described by a single exponential term with a t ½ of approximately 2 min for both the one-chain and two-chain forms of EPA.The clearance and organ distribution of EPA was studied by using 125I-labeled preparations. Following intravenous injection of 125I-1abeled EPA the radioactivity disappeared rapidly from the plasma also with a t ½ of approximately 2 min down to a level of 15 to 20 percent, followed by a small rise of blood radioactivity. Gel filtration of serial samples revealed that the secondary increase of the radioactivity was due to the reappearance of radioactive breakdown products in the blood. Measurement of the organ distribution of 125I at different time intervals revealed that EPA was rapidly accumulated in the liver, followed by a release of degradation products in the blood.Experimental hepatectomy markedly prolonged the half-life of EPA in the blood. Blocking the active site histidine of EPA had no effect on the half-life of EPA in blood nor on the gel filtration patterns of 125I in serial plasma samples.It is concluded that human EPA is rapidly removed from the blood of rabbits by clearance and degradation in the liver. Recognition by the liver does not require a functional active site in the enzyme. Neutralization in plasma by protease inhibitors does not represent a significant pathway of EPA inactivation in vivo.

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Inhibition of Fibrinoid Deposition by Heparin

Thrombosis and Haemostasis ◽

10.1055/s-0038-1654548 ◽

1961 ◽

Vol 06 (01) ◽

pp. 157-159 ◽

Cited By ~ 1

Author(s):

Saul B. Gilson

Keyword(s):

Intravenous Injection ◽

Gamma Globulin ◽

Physiological Significance

ConclusionExperimental glomerulitis in rabbits following intravenous injection of gamma globulin was inhibited by heparinization. The physiological and patho-physiological significance of this observation is considered.

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Lauric Acid-Induced Thrombocytopenia and Thrombosis in Rabbits

Thrombosis and Haemostasis ◽

10.1055/s-0038-1655016 ◽

1967 ◽

Vol 18 (01/02) ◽

pp. 057-065 ◽

Cited By ~ 3

Author(s):

G Zbinden

Keyword(s):

Acid Solution ◽

Intravenous Injection ◽

Lauric Acid ◽

Repeated Administration ◽

Intravenous Dose ◽

Acid Injection ◽

Small Doses ◽

High Doses

SummaryIntravenous injection of 0.5% lauric acid solution into rabbits caused moderate to marked thrombocytopenia. With small doses (2.5 mg/kg) this thrombocyte decrease was reversible and microscopically demonstrable thrombosis in the lungs was only seen or suspected in a small number of rabbits 10 to 30 min after lauric acid injection. High doses were followed by partly reversible thrombocytopenia and by moderate to marked, sometimes lethal, thrombosis in the lungs still demonstrable 24 hrs after injection. Repeated administration of small doses of lauric acid did not lead to a depletion of the circulating thrombocytes. Thrombocytopenic response, however, appeared to be less pronounced after the second and subsequent injections. Studies with Cr51-labeled platelets indicate that during the reversible thrombocytopenia following a small intravenous dose of lauric acid platelets are retained in various organs, particularly the lungs.

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Contribution of Adrenaline to the Fibrinolytic Activity Evoked by E. Coli Endotoxin in the Rat

Thrombosis and Haemostasis ◽

10.1055/s-0038-1665254 ◽

1983 ◽

Vol 50 (02) ◽

pp. 557-559 ◽

Cited By ~ 3

Author(s):

J F Fracasso ◽

A M Rothschild

Keyword(s):

Fatty Acid ◽

Adrenal Gland ◽

Intravenous Injection ◽

Fibrinolytic Activity ◽

Blocking Agent ◽

E Coli ◽

Adrenergic Blocking

SummaryIntravenous injection of E. coli endotoxin (ETX), of adrenaline (AD) or of carbamylcholine (CBCH), caused fibrinolytic activity (FA), directly detectable on plasminogen-rich fibrin plates, to appear in the plasma of the rat. Adrenodemedul- lation abolished responses to ETX or CBCH, but enhanced those to AD. Rats given ETX exhibited marked hypotension, followed by a compensatory phase of normotension abolished by adrenodemedullation and significantly attenuated by phenoxy- benzamine, an a-adrenergic blocking agent which however failed to block FA caused by either ETX or AD. Aspirin, but not indomethacin, inhibited FA evoked by ETX, AD or CBCH. These results suggest that FA evoked by ETX in the rat is caused by AD released from the adrenal gland and does involve the fatty acid cyclooxygenase system.

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RATE OF DISAPPEARANCE OF GROWTH HORMONE FROM THE PLASMA OF RATS AFTER A SINGLE INTRAVENOUS INJECTION

Acta Endocrinologica ◽

10.1530/acta.0.0190181 ◽

1955 ◽

Vol 19 (2) ◽

pp. 181-184 ◽

Cited By ~ 6

Author(s):

Carl A. Gemzell ◽

Frank Heijkenskjöld ◽

Lars Ström

Keyword(s):

Growth Hormone ◽

Intravenous Injection ◽

Single Intravenous Injection

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