Effect of ferrous chloride on biogas production and enzymatic activities during anaerobic fermentation of cow dung and Phragmites straw

2016 ◽  
Vol 27 (2-3) ◽  
pp. 69-82 ◽  
Author(s):  
Huayong Zhang ◽  
Yonglan Tian ◽  
Lijun Wang ◽  
Xueyue Mi ◽  
Yang Chai
Keyword(s):  
Anaerobic Fermentation ◽  
Biogas Production ◽  
Enzymatic Activities ◽  
Cow Dung ◽  
Ferrous Chloride

Metagenomics and Metabolomics Study on Microbial Diversity and Enzymatic Activities Changes on Cow Dung Based Low-Temperature Biogas Production by Entererococcus spp

2021 ◽  
Vol 15 (3) ◽  
pp. 302-311
Author(s):  
Jingping Dai ◽  
Jiang Li ◽  
Wang Zhifang ◽  
Xie Yuqing ◽  
Wang Xiaou ◽  
...  
Keyword(s):  
Low Temperature ◽  
Microbial Diversity ◽  
Biogas Production ◽  
Enzymatic Activities ◽  
Cow Dung ◽  
Study Results ◽  
Metabolomics Study ◽  
Alternative Sources ◽  
Dominant Bacteria ◽  
Glyoxylate Aminotransferase

To fill the gap between the rise in demand for energy and decline in the traditional energy sources such as coal, natural gas and nuclear energy, other alternative sources such as biogas are necessary. Studies have shown that the existing conditions within the fermentation realm control the microbial characteristics in biogas production. However, there is inadequate insight between the duration of fermentation and the microbial diversity, and with specific emphasis to cow manure as the substrate under low temperature fermentation. This study aimed at providing additional insight on the effect of varying fermentation duration (0 to 60 days) on the composition of the dominant microbial flora on cow dung based low-temperature (15 °C) biogas fermentation using metagenomics and metabolomics analyses approach. The study results showed that the main dominant community in the process of methanogenesis are the Spirochaetae, Synergistetes and Chloroflexi, and are new flora in the methane phase. In the peak stage of low-temperature biogas fermentation, the dominant bacteria groups were Methanosarcina and after adding 10% concentration of L1 bacteria. The prediction of metabolic pathway was mainly carbohydrate metabolism and amino acid metabolism with succinyl-CoA synthase a subunit, lactaldehyde reductase and the glutamate-glyoxylate aminotransferase being the main unique enzymes. The study therefore supports the potential of involving the reported dominant microbial communities and related enzymatic activities for improved biogas production under low temperature conditions.

Pretreatment for biogas production by anaerobic fermentation of mixed corn stover and cow dung

Energy ◽  
2012 ◽  
Vol 46 (1) ◽  
pp. 644-648 ◽  
Author(s):  
Shuxia Zhou ◽  
Yulin Zhang ◽  
Yuping Dong
Keyword(s):  
Corn Stover ◽  
Anaerobic Fermentation ◽  
Biogas Production ◽  
Cow Dung

scholarly journals Fermentasi Anaerobik Biogas Dua Tahap Dengan Aklimatisasi dan Pengkondisian pH Fermentasi

2017 ◽  
Vol 1 (1) ◽  
pp. 1 ◽  
Author(s):  
Purwinda Iriani ◽  
Yanti Suprianti ◽  
Fitria Yulistiani
Keyword(s):  
Anaerobic Digestion ◽  
Anaerobic Fermentation ◽  
Biogas Production ◽  
Organic Load ◽  
Cow Dung ◽  
Activation Process ◽  
Two Stage ◽  
One Stage ◽  
Load Rate ◽  
Initial Ph

Produksi biogas pada skala rumah tangga umumnya menggunakan teknologi fermentasi anaerobik di dalam satu biodigester (satu tahap), yang mengakomodasi dua tahap utama prinsip pembentukan biogas, yakni tahap asetogenesis dan tahap metanogenesis. Permasalahan yang muncul dari penggunaan digester biogas satu tahap adalah ketidakseimbangan proses fermentasi (peningkatan laju beban organik, waktu retensi senyawa organik yang lebih cepat, dan produktivitas biogas yang menjadi tidak maksimal). Untuk mengatasi hal tersebut, dilakukan penelitian yang bertujuan melakukan produksi biogas melalui sistem fermentasi anaerobik dua-tahap (two-stage anaerobic digestion), yang didukung dengan pengaturan pH pada proses metanogenik. Pada penelitian ini telah dilakukan proses aklimatisasi (aktivasi) bakteri yang menunjang proses asetogenik dan metanogenik pada skala laboratorium (19 L), dan selanjutnya menjadi inokulum untuk proses fermentasi skala pilot dengan kapasitas biodigester asetogenik 125 L dan metanogenik 500 L. Hasil proses aklimatisasi bakteri asetogenik pada media kotoran sapi menunjukkan adanya kestabilan pH yang dibutuhkan untuk reaksi asetogenik, yaitu pada kisaran pH 5-6, sedangkan kontrol menunjukkan perubahan pH yang masih ada di rentang pH netral yaitu 6-7. Kotoran sapi yang telah melalui proses asetogenik selama 2 minggu (pH awal 5,5), menjadi bahan baku pembuatan biogas pada digester metanogenik. Hasil dari proses metanogenik menunjukkan terjadinya peningkatan volume biogas dan komposisi gas metana (CH4) di dalam biogas. Komposisi CH4 tertinggi diperoleh pada hari ke-20 yakni 74,82% dengan volume produksi biogas tertinggi ada pada hari ke-22, dengan laju 8,87 L/hari. Potensi energi tertinggi yang diperoleh mencapai 217,66 kJ/hari.Generally, biogas production on the household scale is using one-stage anaerobic fermentation technology, which accommodates two main processes of biogas production, namely acetogenesis and methanogenesis. An obstacle of using one-stage biogas digester is the imbalance of the fermentation process that indicated by the increase of organic load rate and shorter retention time that lead to un-optimal biogas productivity. This research undertook the application of two-stage anaerobic digestion, supported by adjusting the initial pH for both acetogenic and methanogenic processes. Firstly, the research initiated by acclimatization (activation) process of acetogenic and methanogenic bacteria via fermentation in laboratory scale (19 L) digesters, separately. The results of acetogenic bacteria acclimatization process on cow dung media showed the pH stability needed for the reaction acetogenic, in the range of 5-6, while the control showed the pH changes still in the neutral pH range (6-7). The substrate from lab-scale acetogenic and methanogenic digester, then used as a starter for pilot-scale digester (125 L and 500 L, respectively). The mixture of water and cow dung were adjusted at initial pH 5.5 on acetogenic digester for 2 weeks. Those material were used for biogas production in the methanogenic digester. The result of the methanogenic process showed an increasing volume of biogas and the composition of methane (CH4) in the biogas. The highest CH4 composition was obtained on the 20th day, which reached 74.82%, and the highest volume of biogas production was at day 22, with the rate of 8.87 L/day. The highest energy potential obtained was 217.66 kJ/day.

scholarly journals Efektivitas Pemanfaatan Serbuk Gergaji dan Limbah Media Tanam Jamur (Baglog) sebagai Bahan Baku Pembuatan Biogas

2016 ◽  
Vol 2 (1) ◽  
pp. 11-16 ◽  
Author(s):  
Dikdik Mulyadi ◽  
Lela Mukmilah Yuningsih ◽  
Desi Kusumawati
Keyword(s):  
Fermentation Process ◽  
Volatile Fatty Acids ◽  
Anaerobic Fermentation ◽  
Biogas Production ◽  
Volume Measurement ◽  
Raw Material ◽  
Cow Dung ◽  
Total Volatile ◽  
A Value ◽  
Volatile Solids

Biogas is  one of energy   that can be produced by anaerobic fermentation of the organic compounds. The objective of this study was to determine the effectiveness of the utilization of waste of media  mushroom growth (baglog) with sawdust as raw material for biogas with  cow dung  activators. The study was conducted through anaerobic fermentation of the samples containing waste baglog (sample 1) and sawdust (sample 2), with the addition of cow manure activator to each sample. Both of these samples do anaerobic fermentation for 32 days, then measuring the volume of biogas every 4 days for 32 days. Methane content  in  samples 1 and 2 measured by  using gas chromatography. To see the effect of the addition of activators cow dung biogas volume measurement was  carried out with  cow dung without addition baglog waste and sawdust. The process of degradation baglog and sawdust with an activator of cow dung could be observed  in  some of the parameters through  total solids (TS), total volatile solids (TVS), volatile fatty acids (VFA), the degree of acidity (pH), and C/N ratio. The results showed that effectiveness of sample 1 resulted in the everage of total volume biogas 28% higher than  sample 2. The content of methane in  sample 1  and sampel 2  was 54% %, and 0.21% respectively. The fermentation process biogas production in this experiment  was carried out  at pH 7, with a value of TS, TVS and VFA showed a decrease  trend after the fermentation process,  C/N ratiowas  lower than the baglog waste sawdust until day 32 retention time. Keywords: Sawdust, baglog waste, biogas, fermentation, methane DOI : http://dx.doi.org/10.15408/jkv.v2i1.3100

scholarly journals Analysis of Potential Biogas Production from a Mixture of Palm Oil Mill Effluent (POME) and Cow Dung

2021 ◽  
Vol 317 ◽  
pp. 04031
Author(s):  
Tiyo Agung Pambudi ◽  
Hadiyanto ◽  
Sri Widodo Agung Suedy
Keyword(s):  
Renewable Energy ◽  
Palm Oil ◽  
Methane Production ◽  
Fermentation Process ◽  
Loading Rate ◽  
Anaerobic Fermentation ◽  
Biogas Production ◽  
Palm Oil Mill Effluent ◽  
Cow Dung ◽  
Palm Oil Mill

POME or palm oil mill effluent is currently still a waste problem that has not been utilized optimally. POME waste has the potential for renewable energy in the form of biogas, but some research results have shown that production is not optimal, so the addition of cow dung needs to be done to increase biogas production because methanogen bacteria found in cow dung help to maximize the anaerobic fermentation process and methane production. This research was conducted to determine the potential for biogas production from a mixture of POME and cow dung for 25 days by conducting a study of the biogas production process. The results of this study indicate that the biogas pressure increases with the addition of the loading rate, which is 101.102 N/m2/day to 101.107 N/m2/day with a daily biogas production of 0, 24247 liters/day with a total accumulation of biogas production for 25 days of 6.1 liters.

scholarly journals The Effect of Digested Manure on Biogas Productivity and Microstructure Evolution of Corn Stalks in Anaerobic Cofermentation

2018 ◽  
Vol 2018 ◽  
pp. 1-10
Author(s):  
Zongyan Lv ◽  
Lei Feng ◽  
Lijie Shao ◽  
Wei Kou ◽  
Peihan Liu ◽  
...  
Keyword(s):  
Microstructure Evolution ◽  
Anaerobic Fermentation ◽  
Biogas Production ◽  
Green Energy ◽  
Lignocellulose Degradation ◽  
Utilization Rate ◽  
Cow Dung ◽  
Multiple Cracks ◽  
Degradation Rates ◽  
Biogas Productivity

The anaerobic fermentation of crop straw and animal wastes is increasingly used for the biogas and green energy generation, as well as reduction of the environmental pollution. The anaerobic cofermentation of corn stalks inoculated by cow dung was found to achieve higher biogas production and cellulose biodegradation. In this study, the effect of mixing corn stalks with cow dung at five different fermentation stages (0, 7, 15, 23, and 31 days of the total fermentation cycle of 60 days) on the further cofermentation process was explored, in order to optimize the corn straw utilization rate and biogas production capacity. In addition, the straw microstructure evolution was investigated by the SEM and XRD methods to identify the optimal conditions for the straw biodegradation process enhancement. The five test groups exhibited nearly identical total biogas productivity values but strongly differed by daily biogas yields (the maximal biogas generation rate being 524.3 ml/d). Based on the degradation characteristics of total solids (TS), volatile solids (VS), and lignocellulose, groups #1 and #3 (0 and 15 days) had the most significant degradation rates of VS (43.73%) and TS (42.07%), respectively, while the largest degradation rates of cellulose (62.70%) and hemicellulose (50.49%) were observed in group #4 (23 days) and group #1 (0 days), respectively. The SEM analysis revealed strong microstructural changes in corn stalks after fermentation manifested by multiple cracks and striations, while the XRD results proved the decrease in peak intensity of cellulose 002 crystal surface and the reduced crystallinity after cofermentation. The results of this study are assumed to be quite instrumental to the further optimization of the corn stalk anaerobic digestion by inoculation with digested manure for lignocellulose degradation enhancement and biogas productivity improvement.

scholarly journals Laser Improves Biogas Production by Anaerobic Digestion of Cow Dung

2018 ◽  
Vol 15 (3) ◽  
pp. 324-327
Author(s):  
Baghdad Science Journal
Keyword(s):  
Hydrogen Sulfide ◽  
Iron Powder ◽  
Anaerobic Fermentation ◽  
Biogas Production ◽  
Laser Pulses ◽  
Optimum Concentration ◽  
Cow Dung ◽  
Waste Biomass ◽  
Ambient Temperatures ◽  
Production Of Hydrogen

This study investigates the digestion of cow dung (CD) for biogas production at laboratory scales. The study was carried out through anaerobic fermentation using cow dung as substrate. The digester was operated at ambient temperatures of 39.5 °C for a period of 10 days. The effect of iron powder in controlling the production of hydrogen sulfide (H2S) has been tested. The optimum concentration of iron powder was 4g/L with the highest biogas production. A Q – swatch Nd:YAG laser has been used to mix and homogenize the components of one of the six digesters and accelerate digestion. At the end of digestion, all digestions effluent was subjected to 5 laser pulses with 250mJ/pules to dispose waste biomass.

scholarly journals New mixtures and technologies for biogas production at biogas plants of agricultural type processing livestock slurry

2009 ◽  
Vol 55 (No. 2) ◽  
pp. 62-68 ◽  
Author(s):  
J. Kára ◽  
Z. Pastorek ◽  
J. Mazancová ◽  
I. Hanzlíková
Keyword(s):  
Waste Water ◽  
Water Treatment ◽  
Anaerobic Fermentation ◽  
Biogas Production ◽  
Waste Water Treatment ◽  
Treatment Plant ◽  
Water Treatment Plant ◽  
Farm Animals ◽  
Waste Water Treatment Plant ◽  
Municipal Sludge

The basis of the biogas production in agriculture is the processing of waste agricultural products (particularly excrements of farm animals but also phytomass). Different but rather similar is the biogas production from biologically degradable municipal waste (BDMW) and biologically degradable industrial waste (BDIW) coming mainly from food industry. The processing of these wastes in agricultural biogas stations could significantly improve their economy. It is necessary to note that all these biogas stations differ from the wastewater cleaning plants where municipal sludge water from public sewers is processed. The municipal sludge water processing to biogas by anaerobic fermentation is a classical technology introduced all over the world. At present, about 100 wastewater cleaning plants operate in the Czech Republic using regular sludge processing into biogas. Electricity produced is utilised mainly for the needs of own operation of waste water treatment plant (WWTP), partly it is sold into public power net. The heat energy is used for heating in the process and its surplus is utilised for operational and administrative facilities. Usually, the heat and electricity quantities produced do not cover the wastewater cleaning plant operation. Agricultural biogas stations and biogas stations for BDMW processing provide considerably higher gas yields because they work with higher dry matter contents in substratum, i.e. 8–12% (compared with waste water treatment plants – 2–6%), and are able to produce high gas surpluses for following applications. Frequently discussed issue are the processing of slaughter waste and grass (or public green areas at biogas stations).

scholarly journals Heavy-Metal Phytoremediation from Livestock Wastewater and Exploitation of Exhausted Biomass

2021 ◽  
Vol 18 (5) ◽  
pp. 2239
Author(s):  
Monika Hejna ◽  
Elisabetta Onelli ◽  
Alessandra Moscatelli ◽  
Maurizio Bellotto ◽  
Cinzia Cristiani ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Water Conservation ◽  
Anaerobic Fermentation ◽  
Biogas Production ◽  
Animal Health ◽  
Livestock Production ◽  
Animal Production ◽  
Feed Additives ◽  
Livestock Wastewater

Sustainable agriculture is aimed at long-term crop and livestock production with a minimal impact on the environment. However, agricultural practices from animal production can contribute to global pollution due to heavy metals from the feed additives that are used to ensure the nutritional requirements and also promote animal health and optimize production. The bioavailability of essential mineral sources is limited; thus, the metals are widely found in the manure. Via the manure, metallic ions can contaminate livestock wastewater, drastically reducing its potential recycling for irrigation. Phytoremediation, which is an efficient and cost-effective cleanup technique, could be implemented to reduce the wastewater pollution from livestock production, in order to maintain the water conservation. Plants use various strategies for the absorption and translocation of heavy metals, and they have been widely used to remediate livestock wastewater. In addition, the pollutants concentrated in the plants can be exhausted and used as heat to enhance plant growth and further concentrate the metals, making recycling a possible option. The biomass of the plants can also be used for biogas production in anaerobic fermentation. Combining phytoremediation and biorefinery processes would add value to both approaches and facilitate metal recovery. This review focuses on the concept of agro-ecology, specifically the excessive use of heavy metals in animal production, the various techniques and adaptations of the heavy-metal phytoremediation from livestock wastewater, and further applications of exhausted phytoremediated biomass.

Enhancing biogas production of cow dung during anaerobic digestion using nanoferrites

2021 ◽  
Author(s):  
Mahmoud A. Sliem ◽  
Sara El-Ansary ◽  
Wafaaa Soliman ◽  
Yehia Badr
Keyword(s):  
Anaerobic Digestion ◽  
Biogas Production ◽  
Cow Dung
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