Microbial reduction of Cr(VI) in the presence of Ni, Cu and Zn by bacterial consortium enriched from an electroplating contaminated site

2021 ◽  
Author(s):  
Wen-Jing Gong ◽  
Xing-Run Wang ◽  
He-Ping Zhao
Keyword(s):  
Bacterial Consortium ◽  
Microbial Reduction ◽  
Contaminated Site ◽  
Cu And Zn

scholarly journals Biodegradation of Extractable Petroleum Hydrocarbons by Consortia Bacillus cereus and Pseudomonas putida in Petroleum Contaminated-Soil

2019 ◽  
Vol 19 (2) ◽  
pp. 347 ◽  
Author(s):  
Abubakar Tuhuloula ◽  
Suprapto Suprapto ◽  
Ali Altway ◽  
Sri Rachmania Juliastuti
Keyword(s):  
Bacillus Cereus ◽  
Pseudomonas Putida ◽  
Contaminated Soil ◽  
Petroleum Hydrocarbons ◽  
Bacterial Consortium ◽  
Contaminated Site ◽  
Oil Concentration ◽  
Petroleum Contaminated Soil ◽  
And Control ◽  
Water Ratio

Contamination of soil by the activities of exploration, production, and disposal of oil waste into the environment causes serious damage to the environmental ecosystem, the target of processing by the bacteria as a model for remediation of oil contaminated site. Thus, the study was focused on determining the biodegradation percentage of extractable petroleum hydrocarbons as a function of the oil concentration. This research was conducted in a slurry bioreactor with mixed contaminated soil to water ratio of 20:80 (wt.%). A consortium of Bacillus cereus and Pseudomonas putida bacteria 10% (v/v) and 15% (v/v) with the ratio of 2:3, 1:1, and 3:2 was inserted into the slurry bioreactor and a single reactor was used as a control. The result of identification with an initial concentration of extractable petroleum hydrocarbons of 299.53 ng/µL, after 49 days of incubation for bacterial consortium 10% (v/v), the concentration was reduced to 85.31; 32.43; 59.74; and 112.22 ng/µL respectively and the biodegradation percentage was 71.5; 89.17; 80.05; and 62.54%. As for the bacterial consortium concentration of 15% (v/v) with the same ratio and control, the effluent concentration was 12.48; 7.72; 18.93 ng/µL, respectively or the biodegradation percentage was 95.83; 97.42; 93.68%.

scholarly journals Accumulation of Pb, Fe, Mn, Cu and Zn in plants and choice of hyperaccumulator plant in the industrial town of Vian, Iran

2011 ◽  
Vol 63 (3) ◽  
pp. 739-745 ◽  
Author(s):  
B. Lorestani ◽  
M. Cheraghi ◽  
N. Yousefi
Keyword(s):  
Heavy Metal ◽  
Contaminated Soils ◽  
Industrial Area ◽  
Contaminated Site ◽  
Metal Hyperaccumulation ◽  
Major Step ◽  
Industrial Town ◽  
High Concentrations ◽  
Hyperaccumulator Plant ◽  
Cu And Zn

Various industrial activities contribute heavy metals to the soil environment directly or indirectly through the release of solid wastes, waste gases, and wastewater. Phytoremediation can be potentially used to remedy metal-contaminated sites. A major step towards the development of phytoremediation of heavy metal-impacted soils is the discovery of the heavy metal hyperaccumulation in plants. This study evaluated the potential of 7 species growing on a contaminated site in an industrial area. Several established criteria to define a hyperaccumulator plant were applied. The case study was represented by an industrial town in the Hamedan province in the central-western part of Iran. This study showed that most of the sampled species were able to grow in heavily metal-contaminated soils and were also able to accumulate extraordinarily high concentrations of some metals such as Pb, Fe, Mn, Cu and Zn. Based on the obtained results and using the most common criteria, Camphorosma monospeliacum for Pb and Fe, and Salsola soda and Circium arvense for Pb can be classified as hyperaccumulators and, therefore, they have suitable potential for the phytoremediation of contaminated soils.

scholarly journals How the Soil Microbial Communities and Activities Respond to Long-Term Heavy Metal Contamination in Electroplating Contaminated Site

2021 ◽  
Vol 9 (2) ◽  
pp. 362 ◽  
Author(s):  
Wen-Jing Gong ◽  
Zi-Fan Niu ◽  
Xing-Run Wang ◽  
He-Ping Zhao
Keyword(s):  
Heavy Metal ◽  
Microbial Communities ◽  
Metal Contamination ◽  
Heavy Metal Contamination ◽  
Soil Microbial Communities ◽  
Contaminated Site ◽  
Rrna Gene ◽  
Soil Microbial ◽  
Cu And Zn

The effects of long-term heavy metal contamination on the soil biological processes and soil microbial communities were investigated in a typical electroplating site in Zhangjiakou, China. It was found that the soil of the electroplating plant at Zhangjiakou were heavily polluted by Cr, Cr (VI), Ni, Cu, and Zn, with concentrations ranged from 112.8 to 9727.2, 0 to 1083.3, 15.6 to 58.4, 10.8 to 510.0 and 69.6 to 631.6 mg/kg, respectively. Soil urease and phosphatase activities were significantly inhibited by the heavy metal contamination, while the microbial biomass carbon content and the bacterial community richness were much lower compared to noncontaminated samples, suggesting that the long-term heavy metal contamination had a severe negative effect on soil microorganisms. Differently, soil dehydrogenase was promoted in the presence of Chromate compared to noncontaminated samples. This might be due to the enrichment of Sphingomonadaceae, which have been proven to be able to secrete dehydrogenase. The high-throughput sequencing of the 16S rRNA gene documented that Proteobacteria, Actinobacteria, and Chloroflexi were the dominant bacterial phyla in the contaminated soil. The Spearman correlation analysis showed the Methylobacillus, Muribaculaceae, and Sphingomonadaceae were able to tolerate high concentrations of Cr, Cr (VI), Cu, and Zn, indicating their potential in soil remediation.

Acid Leaching of Cu and Zn from a Smelter Slag with a Bacterial Consortium

2015 ◽  
Vol 1130 ◽  
pp. 660-663 ◽  
Author(s):  
Olli H. Tuovinen ◽  
Silja Särkijärvi ◽  
Esa Peuraniemi ◽  
Saku Junnikkala ◽  
Jaakko A. Puhakka ◽  
...  
Keyword(s):  
Slag Sample ◽  
Acid Leaching ◽  
Bacterial Consortium ◽  
Growth Media ◽  
Dissolved Metals ◽  
Test Culture ◽  
Shake Flasks ◽  
Leaching Experiments ◽  
Cu And Zn ◽  
Smelter Slag

This study was undertaken to determine the leaching of Cu and Zn from a smelter slag in acidic, sulfate-rich solutions with, and without, mesophilic Fe-and S-oxidizing bacteria. Cu in the slag was mostly distributed in fayalite, Fe-deficient Cu-sulfides and metallic Cu, and Zn was associated with fayalite, magnetite and glassy silicates. The test culture was enriched from acid mine water using mineral salt growth media supplemented with 4.5 g Fe2+ L-1 and 10 g S0 L-1. The bioleaching experiments were carried out in shake flasks at 27 °C and chemical controls were included. The slag sample was pre-leached for 24 h to partially satisfy the acid demand before the leaching experiments at 10% pulp density. In the bioleaching experiments, 69-83% Cu and 4.1-14% Zn were dissolved in 25 days at pH 2.0-2.2. The highest extent of bioleaching was with S0 as the substrate. The efficiency of the bioleaching depended on H2SO4 generation from the added S0. The activities of the bacteria were not adversely affected by the dissolved metals. The maximum yields of chemical leaching were 68% Cu and 3.7% Zn.

scholarly journals Metagenomic insight into the plant growth-promoting potential of a diesel-degrading bacterial consortium for enhanced rhizoremediation application

2021 ◽  
Author(s):  
Michael O Eze ◽  
Volker Thiel ◽  
Grant C Hose ◽  
Simon C George ◽  
Rolf Daniel
Keyword(s):  
Plant Growth ◽  
Organic Pollutants ◽  
Dna Sequences ◽  
Bacterial Consortium ◽  
Contaminated Site ◽  
Metagenome Analysis ◽  
Remediation Strategies ◽  
Plant Growth Promoting ◽  
Enhanced Bioremediation ◽  
Growth Promoting

The slow rate of natural attenuation of organic pollutants, together with unwanted environmental impacts of traditional remediation strategies, has necessitated the exploration of plant-microbe systems for enhanced bioremediation applications. The identification of microorganisms capable of promoting both plant growth and hydrocarbon degradation is crucial to the success of plant-based remediation techniques. Through successive enrichments of a soil sample from a historic oil-contaminated site in Wietze, Germany, we isolated a plant growth-promoting and hydrocarbon-degrading bacterial consortium. Metagenome analysis of the consortium led to the identification of genes and taxa putatively associated with these processes. The majority of the coding DNA sequences involved in these reactions were affiliated to Acidocella aminolytica and Acidobacterium capsulatum. In microcosm experiments performed in association with Medicago sativa L., the consortium achieved 91% rhizodegradation of diesel fuel hydrocarbons within 60 days, indicating its potential for biotechnological applications in the remediation of sites contaminated by organic pollutants.

scholarly journals Treatment of Port Harcourt Refinery Effluent by a Bacterial Consortium Immobilized on Agro-based Bio Carriers

2019 ◽  
pp. 1-10
Author(s):  
G. C. Iheanacho ◽  
A. A. Ibiene ◽  
P. O. Okerentugba
Keyword(s):  
Bacterial Biomass ◽  
Bacterial Consortium ◽  
Contaminated Site ◽  
Free Form ◽  
Polluted Water ◽  
Refinery Wastewater ◽  
Bacterial Isolates ◽  
Oil And Grease ◽  
Port Harcourt ◽  
Immobilized Consortium

Discharge of poorly treated refinery wastewater has always been a major environmental challenge. Bacterial immobilization is key to the maintenance of biomass on a contaminated site. In this study, a mixed culture of three bacterial isolates from oil-polluted water: Pseudomonas aeruginosa (MN294989), Bacillus tequilensis (MN294990) and Micrococcus sp. immobilized on Groundnut Shell (GS), Melon Husk (MH) and Sugarcane Bagasse (SB) were employed in the bioremediation of Port Harcourt refinery wastewater. Surface area and pore size distribution of the agro-based bio carriers were suitable for bacteria adhesion. The bacterial isolates were screened for phenol, naphthalene and hydrocarbon utilization. Scanning Electron Microscopy (SEM) was used to ascertain the immobilization of the consortium on the agro-base carriers. A 15-days laboratory-scale treatment of refinery raw wastewater was compared in the immobilised and immobilized consortium. The agro-based residue immobilized consortium enhanced the reduction in BOD5, COD, oil and grease, phenol by 7%, 9%, 30% and 5% respectively compared to the free form of the consortium. This study underscores the role of immobilization in maintaining high bacterial biomass on contaminated site and possible improvement in bioremediation of refinery wastewater.

scholarly journals Diversity and metagenome analysis of a hydrocarbon-degrading bacterial consortium from asphalt lakes located in Wietze, Germany

AMB Express ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Michael O. Eze ◽  
Grant C. Hose ◽  
Simon C. George ◽  
Rolf Daniel
Keyword(s):  
Diesel Fuel ◽  
Organic Contaminants ◽  
Cost Effective ◽  
Bacterial Consortium ◽  
Contaminated Site ◽  
Rrna Gene ◽  
16S Rrna Gene Analysis ◽  
Metagenome Analysis ◽  
Environmental Threat ◽  
Environmentally Sensitive

AbstractThe pollution of terrestrial and aquatic environments by petroleum contaminants, especially diesel fuel, is a persistent environmental threat requiring cost-effective and environmentally sensitive remediation approaches. Bioremediation is one such approach, but is dependent on the availability of microorganisms with the necessary metabolic abilities and environmental adaptability. The aim of this study was to examine the microbial community in a petroleum contaminated site, and isolate organisms potentially able to degrade hydrocarbons. Through successive enrichment of soil microorganisms from samples of an historic petroleum contaminated site in Wietze, Germany, we isolated a bacterial consortium using diesel fuel hydrocarbons as sole carbon and energy source. The 16S rRNA gene analysis revealed the dominance of Alphaproteobacteria. We further reconstructed a total of 18 genomes from both the original soil sample and the isolated consortium. The analysis of both the metagenome of the consortium and the reconstructed metagenome-assembled genomes show that the most abundant bacterial genus in the consortium, Acidocella, possess many of the genes required for the degradation of diesel fuel aromatic hydrocarbons, which are often the most toxic component. This can explain why this genus proliferated in all the enrichment cultures. Therefore, this study reveals that the microbial consortium isolated in this study and its dominant genus, Acidocella, could potentially serve as an effective inoculum for the bioremediation of sites polluted with diesel fuel or other organic contaminants.

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