scholarly journals Treatment of Port Harcourt Refinery Effluent by a Bacterial Consortium Immobilized on Agro-based Bio Carriers

2019 ◽  
pp. 1-10
Author(s):  
G. C. Iheanacho ◽  
A. A. Ibiene ◽  
P. O. Okerentugba
Keyword(s):  
Bacterial Biomass ◽  
Bacterial Consortium ◽  
Contaminated Site ◽  
Free Form ◽  
Polluted Water ◽  
Refinery Wastewater ◽  
Bacterial Isolates ◽  
Oil And Grease ◽  
Port Harcourt ◽  
Immobilized Consortium

Discharge of poorly treated refinery wastewater has always been a major environmental challenge. Bacterial immobilization is key to the maintenance of biomass on a contaminated site. In this study, a mixed culture of three bacterial isolates from oil-polluted water: Pseudomonas aeruginosa (MN294989), Bacillus tequilensis (MN294990) and Micrococcus sp. immobilized on Groundnut Shell (GS), Melon Husk (MH) and Sugarcane Bagasse (SB) were employed in the bioremediation of Port Harcourt refinery wastewater. Surface area and pore size distribution of the agro-based bio carriers were suitable for bacteria adhesion. The bacterial isolates were screened for phenol, naphthalene and hydrocarbon utilization. Scanning Electron Microscopy (SEM) was used to ascertain the immobilization of the consortium on the agro-base carriers. A 15-days laboratory-scale treatment of refinery raw wastewater was compared in the immobilised and immobilized consortium. The agro-based residue immobilized consortium enhanced the reduction in BOD5, COD, oil and grease, phenol by 7%, 9%, 30% and 5% respectively compared to the free form of the consortium. This study underscores the role of immobilization in maintaining high bacterial biomass on contaminated site and possible improvement in bioremediation of refinery wastewater.

Isolation and Characterization of Cellulose Degrading Bacteria of Termite Gut from North Eastern Region of India

2016 ◽  
Vol 5 (6) ◽  
pp. 283-290
Author(s):  
Sonika Sharma ◽  
Soumya Chatterjee ◽  
Sibnarayan Datta ◽  
Rajesh Kumar Prasad ◽  
Angkita Sharma ◽  
...  
Keyword(s):  
Agar Plate ◽  
Bacterial Consortium ◽  
Cellulase Production ◽  
Eastern Region ◽  
Bacterial Isolates ◽  
Carbohydrate Utilization ◽  
North East ◽  
Isolation And Characterization ◽  
Degrading Bacteria ◽  
Termite Gut

A study was conducted to screen and isolate cellulase producing bacteria of termite gut from North east region of India. A total of 27 culturable bacterial isolates were screened for cellulase production. Out of the 27 bacterial isolates 11 showed zone of clearance on CMC agar media on staining with 1% Congo red, suggesting potential cellulose degrading activity. The maximum hydrolysis capacities (HC value) on CMC agar plate was found within the range of 3.6 to 40mm. The morphological characterization and gram staining of the positive isolates indicated that 3 isolates were positively stained rods and others were negative cocci. All the cellulase positive isolates were also tested for carbohydrate utilization with maltose, dextrose and fructose, to which all the 11 isolates responded positively. Further, based on the amplification and sequencing of the 16S rRNA genetic region, isolates were identified as member of the genus Bacillus, Paenibacillus and Staphylococcus. The degrading potential of these bacteria were assessed by developing bacterial consortium and efficient degradation was reported after seven days of incubation with different cellulose source like rice, cotton and rice husk.

Degradation of phenol at high concentrations using immobilization of Pseudomonas putida P53 into sawdust entrapped in sodium-alginate beads

2019 ◽  
Vol 79 (7) ◽  
pp. 1387-1396 ◽  
Author(s):  
Moslem Abarian ◽  
Mehdi Hassanshahian ◽  
Akramsadat Esbah
Keyword(s):  
Sodium Alginate ◽  
Bacterial Biomass ◽  
Alginate Beads ◽  
Free Form ◽  
Sem Images ◽  
Phenol Biodegradation ◽  
Degrading Bacteria ◽  
The Matrix ◽  
High Concentrations ◽  
Positive Results

Abstract Phenols are distributed either as natural or artificial mono-aromatic compounds in various environmental sites as major pollutants. The objective of this study was the immobilization of the phenol degrading bacteria P. putida P53 and A. scleromae P69 in sodium-alginate beads and sawdust as carriers and evaluate the biodegradation ability. The biodegradation ability of strains in free form were evaluated and P. putida P53 was shown to biodegrade up to 1,800 mg/L phenol. Bacterial biomass was prepared and attached to carrier with entrapment and attachment methods. Prepared beads were added to Erlenmeyer flasks containing different concentrations of phenol in BH medium (1,800, 2,200, 2,600 and 3,000 mg/L). According to the results, phenol biodegradation efficiency of immobilized bacteria in sawdust was more than free form. Strain P53 had better biodegradation than P69 strain. Attachment and entrapments into carriers had positive results, Scanning electron micrograph (SEM) images indicated that alginate beads were globular shapes (10 nm), and strains aggregated between the large cavities of the matrix. Comparison of sawdust and alginate as carriers for degradation of phenol at high concentrations demonstrated that sawdust improved biodegradation better, and immobilized P53 into sawdust entrapped in sodium-alginate beads can be used for biodegradation purposes.

Biodegradation of low and high molecular weight hydrocarbons in petroleum refinery wastewater by a thermophilic bacterial consortium

2016 ◽  
Vol 38 (19) ◽  
pp. 2381-2391 ◽  
Author(s):  
Arulazhagan Pugazhendi ◽  
Hadeel Abbad Wazin ◽  
Huda Qari ◽  
Jalal Mohammad Al-Badry Basahi ◽  
Jean Jacques Godon ◽  
...  
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Petroleum Refinery ◽  
Bacterial Consortium ◽  
Refinery Wastewater ◽  
Petroleum Refinery Wastewater

scholarly journals Biodegradation of Extractable Petroleum Hydrocarbons by Consortia Bacillus cereus and Pseudomonas putida in Petroleum Contaminated-Soil

2019 ◽  
Vol 19 (2) ◽  
pp. 347 ◽  
Author(s):  
Abubakar Tuhuloula ◽  
Suprapto Suprapto ◽  
Ali Altway ◽  
Sri Rachmania Juliastuti
Keyword(s):  
Bacillus Cereus ◽  
Pseudomonas Putida ◽  
Contaminated Soil ◽  
Petroleum Hydrocarbons ◽  
Bacterial Consortium ◽  
Contaminated Site ◽  
Oil Concentration ◽  
Petroleum Contaminated Soil ◽  
And Control ◽  
Water Ratio

Contamination of soil by the activities of exploration, production, and disposal of oil waste into the environment causes serious damage to the environmental ecosystem, the target of processing by the bacteria as a model for remediation of oil contaminated site. Thus, the study was focused on determining the biodegradation percentage of extractable petroleum hydrocarbons as a function of the oil concentration. This research was conducted in a slurry bioreactor with mixed contaminated soil to water ratio of 20:80 (wt.%). A consortium of Bacillus cereus and Pseudomonas putida bacteria 10% (v/v) and 15% (v/v) with the ratio of 2:3, 1:1, and 3:2 was inserted into the slurry bioreactor and a single reactor was used as a control. The result of identification with an initial concentration of extractable petroleum hydrocarbons of 299.53 ng/µL, after 49 days of incubation for bacterial consortium 10% (v/v), the concentration was reduced to 85.31; 32.43; 59.74; and 112.22 ng/µL respectively and the biodegradation percentage was 71.5; 89.17; 80.05; and 62.54%. As for the bacterial consortium concentration of 15% (v/v) with the same ratio and control, the effluent concentration was 12.48; 7.72; 18.93 ng/µL, respectively or the biodegradation percentage was 95.83; 97.42; 93.68%.

scholarly journals Antimicrobial Activity of Honey on Some Bacterial Isolates from Selected Brands of Sachet Water Sold within Port Harcourt, Nigeria

2020 ◽  
pp. 40-46
Author(s):  
V. N. Agi ◽  
C. P. Aleru ◽  
C. A. Azike ◽  
O. A. Ollor ◽  
D. U. Alonyenu
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Enterococcus Faecalis ◽  
Water Samples ◽  
Staphylococcus Epidermidis ◽  
Bacillus Species ◽  
Bacterial Isolates ◽  
Zone Of Inhibition ◽  
Port Harcourt ◽  
Sachet Water

Background: The present study was carried out to assess the antimicrobial effect of honey on bacterial isolates from sachet water sold within Eligbolo Community in Port Harcourt, Nigeria. Methodology: Five brands of sachet water commonly consumed by the people living in Eligbolo Community of Port Harcourt, Nigeria were purchased from different Vendors in the community. Nutrient and MacConkey agar plates were used for culturing of water samples using spread plate method. Ten-fold serial dilution and Most Probable Number (MPN) were among the methods used and the samples analyzed were according to standard procedures. Natural honey purchased from Ogbokolo in Benue State, Nigeria was used for susceptibility testing. Quality control, ant inhibition and water test methods were performed using the honey to confirm its originality before use. Antimicrobial sensitivity testing was done using the agar well diffusion method. Results: Results obtained showed the bacterial isolated from the 5 sachet brands of water. These include Bacillus species 5 (62.5%), Enterococcus faecalis 1 (12.5%), Staphylococcus epidermidis 1 (12.5%), and Escherichia coli 1 (12.5%). All of the 5 sachet water samples analyzed failed to meet the WHO drinking water standard of zero coliform per 100 ml making them unsuitable for human consumption. Faecal coliform was isolated from sample C indicating faecal contamination of the drinking water. The sensitivity of the isolates to the honey sample showed higher zone of inhibition compared to the standard antibiotic used as control. Staphylococcus epidermidis showed the highest zone of inhibition (39 mm), followed by Escherichia coli (37 mm), Bacillus species (35 mm) and Enterococcus faecalis (32 mm) respectively. Conclusion: The results revealed that honey has a broad antimicrobial spectrum against Gram positive and Gram negative bacteria and could provide alternative agent to overcome the problem of increasingly bacteria resistance to synthetic antimicrobial agents. It is therefore, recommended that further work should be encouraged for the extraction of the crude components of honey and their use for antibiotic production.

scholarly journals Methyl Orange Biodegradation by Immobilized Consortium Microspheres: Experimental Design Approach, Toxicity Study and Bioaugmentation Potential

Biology ◽  
2022 ◽  
Vol 11 (1) ◽  
pp. 76
Author(s):  
Amany Ibrahim ◽  
Esmail M. El-Fakharany ◽  
Marwa M. Abu-Serie ◽  
Marwa F. ElKady ◽  
Marwa Eltarahony
Keyword(s):  
Methyl Orange ◽  
Degradation Products ◽  
Industrial Effluents ◽  
Bacterial Consortium ◽  
Alginate Beads ◽  
Photosynthetic Performance ◽  
Normal Lung ◽  
Vis Spectroscopy ◽  
Augmented Systems ◽  
Immobilized Consortium

Methyl orange (MO) is categorized among the recalcitrant and refractory xenobiotics, representing a significant burden in the ecosystem. To clean-up the surrounding environment, advances in microbial degradation have been made. The main objective of this study was to investigate the extent to which an autochthonous consortium immobilized in alginate beads can promote an efficient biodegradation of MO. By employing response surface methodology (RSM), a parametric model explained the interaction of immobilized consortium (Raoultella planticola, Ochrobactrum thiophenivorans, Bacillus flexus and Staphylococcus xylosus) to assimilate 200 mg/L of MO in the presence of 40 g/L of NaCl within 120 h. Physicochemical analysis, including UV-Vis spectroscopy and FTIR, and monitoring of the degrading enzymes (azoreductase, DCIP reductase, NADH reductase, laccase, LiP, MnP, nitrate reductase and tyrosinase) were used to evaluate MO degradation. In addition, the toxicity of MO-degradation products was investigated by means of phytotoxicity and cytotoxicity. Chlorella vulgaris retained its photosynthetic performance (>78%), as shown by the contents of chlorophyll-a, chlorophyll-b and carotenoids. The viability of normal lung and kidney cell lines was recorded to be 90.63% and 99.23%, respectively, upon exposure to MO-metabolic outcomes. These results reflect the non-toxicity of treated samples, implying their utilization in ferti-irrigation applications and industrial cooling systems. Moreover, the immobilized consortium was employed in the bioremediation of MO from artificially contaminated agricultural and industrial effluents, in augmented and non-augmented systems. Bacterial consortium remediated MO by 155 and 128.5 mg/L in augmented systems of agricultural and industrial effluents, respectively, within 144 h, revealing its mutual synergistic interaction with both indigenous microbiotas despite differences in their chemical, physical and microbial contents. These promising results encourage the application of immobilized consortium in bioaugmentation studies using different resources.

scholarly journals Antibiogram of Bacteria Isolated in the Air of Some Public Toilets in Port Harcourt Metropolis, Rivers State, Nigeria

2019 ◽  
pp. 1-6
Author(s):  
Williams, Janet Olufunmilayo ◽  
Lumati, Jessica
Keyword(s):  
Indoor Air ◽  
Antimicrobial Susceptibility ◽  
Health Sector ◽  
Bacterial Isolates ◽  
Susceptibility Pattern ◽  
Klebsiella Species ◽  
Antimicrobial Susceptibility Pattern ◽  
Port Harcourt ◽  
Study Sites ◽  
Public Toilets

Antimicrobial resistance is one of the major challenges facing the health sector. This study was aimed at investigating the antimicrobial susceptibility pattern of bacterial isolates from indoor air of public toilets in motor parks with a view of developing an antibiogram. The study sites included the public toilets in Mile 3 Motor Park, Rivers Transport Company (RTC) park and a General Motor Park in Waterlines, Port Harcourt, Nigeria. The indoor air was sampled using the plate exposure and disc diffusion techniques in determining the antimicrobial susceptibility pattern of the bacterial isolates. The bacterial isolates in the genera, Staphylococcus, Bacillus, Providencia, Pseudomonas, Escherichia, Enterobacter and Klebsiella species were identified.  The antibiogram of the bacteria isolated showed that the bacterial isolates exhibited multi-drug resistant species as the isolates were resistant to more than two antibiotics. Ciprofloxacin and Tarivid are the drug of choice and recommended for treatment of infections from these study sites.

scholarly journals Biodeterioration of Classroom Wall Surfaces in the University of Port Harcourt, Nigeria

2019 ◽  
pp. 1-8
Author(s):  
B. C. Anele ◽  
P. O. Okerentugba ◽  
H. O. Stanley ◽  
C. J. Ugboma
Keyword(s):  
Physicochemical Parameters ◽  
Biochemical Characterization ◽  
Diffusion Method ◽  
Frequency Of Occurrence ◽  
Bacterial Isolates ◽  
Port Harcourt ◽  
Aspergillus Fumigates ◽  
Bacillus Spp ◽  
Antibiotics Susceptibility ◽  
The University

Aim: This study investigated the biodeterioration of classroom wall surfaces in the University of Port Harcourt, Nigeria. Study Design: Scrapings from selected classroom wall surfaces were analyzed for their microbiological and physicochemical parameters. Isolated bacteria were screened for their antibiotics susceptibility. Place and Duration of Study: This study was carried out at the University of Port Harcourt between March - June 2018. Methods: The population of culturable bacterial and fungal biodeteriogens was determined by plating. Physicochemical parameters were determined using standard methods. Antibiotic susceptibility pattern of the bacterial isolates was determined using the disc diffusion method. Results: The total culturable heterotrophic bacterial counts ranged from 6.48 to 8.23 log CFU/g while the total fungal counts ranged from 5.00 to 7.28 log CFU/g. The bacterial isolates identified by biochemical characterization and their frequency of occurrence are Micrococcus spp. (7.3%), Citrobacter spp. (3.2%), Bacillus spp. (39.1%), Serratia spp. (3.2%), Corynebacterium spp. (10.9%), Staphylococcus aureus (20.1%), Proteus spp. (9.2%) and Shigella spp (7.0%). The fungal isolates and their frequency of occurrence are Aspergillus flavus (39.1%), Penicillium spp. (20.1%), Microsporium canis (14.3%), Coccidioides spp. (10.9%), Aspergillus fumigates (3.2%) and Tricophyton spp (3.2%). All antibiotics used showed activity against all bacterial isolates except Proteus spp. From the results of the physicochemical parameters, pH values ranged from 6.15 to 9.01, nitrate ranged from 5.30 to 14.83 mg/kg, phosphate ranged 2.19 to 5.94 mg/kg, sulphate ranged from 12.97 to 19.07 mg/kg and Total Organic Carbon ranged from 74.89 to 119.43 mg/kg. Conclusions: This study has shown the potential public health risk associated with classroom building deterioration owing to the presence of pathogenic microorganisms. Therefore, measures towards prevention and mitigation of classroom building biodeterioration should be in place.

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