Chitosan–bacterial nanocellulose nanofibrous structures for potential wound dressing applications

Abstract Bacterial nanocellulose (BNC) is a type of 3-dimensionally structured polymer gel produced by Acetobacter that has recently attracted increased interest in wound healing concerns. To produce an effective antibacterial wound dressing, researchers investigated the manufacturing and structural features of honey-infused BNC reinforced gelatin/aldehyde-modified Guar gum films (H/BNC/Ge/AD-GG). Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), mechanical characteristics, water solubility, and degradability were all used to assess the produced films. In addition, the influence of honey addition on the produced films' various properties has been examined. Antibacterial activity, better degradation capability, improved mechanical qualities, and excellent cell adhesion and proliferation by NIH-3T3 fibroblast cells were among the outcomes. The cytotoxicity assay in vitro revealed good cytocompatibility. As a result of the findings, the produced H/BNC/Ge/AD-GG films appear to have a high potential for antibacterial wound dressing applications.

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Controlled Release of the α-Tocopherol-Derived Metabolite α-13′-Carboxychromanol from Bacterial Nanocellulose Wound Cover Improves Wound Healing

Nanomaterials ◽

10.3390/nano11081939 ◽

2021 ◽

Vol 11 (8) ◽

pp. 1939

Author(s):

Jessica Hoff ◽

Berit Karl ◽

Jana Gerstmeier ◽

Uwe Beekmann ◽

Lisa Schmölz ◽

...

Keyword(s):

Wound Healing ◽

Controlled Release ◽

Wound Dressing ◽

Pharmaceutical Formulations ◽

Bioactive Molecules ◽

Bacterial Nanocellulose ◽

Cellular Effects ◽

Persistent Inflammation ◽

Advanced Therapy ◽

Anti Inflammatory

Inflammation is a hallmark of tissue remodeling during wound healing. The inflammatory response to wounds is tightly controlled and well-coordinated; dysregulation compromises wound healing and causes persistent inflammation. Topical application of natural anti-inflammatory products may improve wound healing, in particular under chronic pathological conditions. The long-chain metabolites of vitamin E (LCM) are bioactive molecules that mediate cellular effects via oxidative stress signaling as well as anti-inflammatory pathways. However, the effect of LCM on wound healing has not been investigated. We administered the α-tocopherol-derived LCMs α-13′-hydroxychromanol (α-13′-OH) and α-13′-carboxychromanol (α-13′-COOH) as well as the natural product garcinoic acid, a δ-tocotrienol derivative, in different pharmaceutical formulations directly to wounds using a splinted wound mouse model to investigate their effects on the wounds’ proinflammatory microenvironment and wound healing. Garcinoic acid and, in particular, α-13′-COOH accelerated wound healing and quality of the newly formed tissue. We next loaded bacterial nanocellulose (BNC), a valuable nanomaterial used as a wound dressing with high potential for drug delivery, with α-13′-COOH. The controlled release of α-13′-COOH using BNC promoted wound healing and wound closure, mainly when a diabetic condition was induced before the injury. This study highlights the potential of α-13′-COOH combined with BNC as a potential active wound dressing for the advanced therapy of skin injuries.

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Response to: Holzer JCJ, Tiffner K, Kainz S, Reisenegger P, Bernardelli de Mattos I, Funk M, et al. A novel human ex-vivo burn model and the local cooling effect of a bacterial nanocellulose-based wound dressing. Burns 2020;46:1924–32

Burns ◽

10.1016/j.burns.2021.06.012 ◽

2021 ◽

Author(s):

Edmund Hugh Wright

Keyword(s):

Wound Dressing ◽

Ex Vivo ◽

Cooling Effect ◽

Local Cooling ◽

Bacterial Nanocellulose

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Fabrication and evaluation of bacterial nanocellulose/poly(acrylic acid)/graphene oxide composite hydrogel: Characterizations and biocompatibility studies for wound dressing

Journal of Biomedical Materials Research Part B Applied Biomaterials ◽

10.1002/jbm.b.34309 ◽

2019 ◽

Vol 107 (6) ◽

pp. 2140-2151 ◽

Cited By ~ 7

Author(s):

Xiang Yi Chen ◽

Hao Ran Low ◽

Xin Yi Loi ◽

Laura Merel ◽

Mohd Amin Mohd Cairul Iqbal

Keyword(s):

Graphene Oxide ◽

Acrylic Acid ◽

Wound Dressing ◽

Composite Hydrogel ◽

Bacterial Nanocellulose ◽

Oxide Composite ◽

Poly Acrylic Acid

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A novel human ex-vivo burn model and the local cooling effect of a bacterial nanocellulose-based wound dressing

Burns ◽

10.1016/j.burns.2020.06.024 ◽

2020 ◽

Vol 46 (8) ◽

pp. 1924-1932 ◽

Cited By ~ 3

Author(s):

Judith C.J. Holzer ◽

Katrin Tiffner ◽

Sonja Kainz ◽

Peter Reisenegger ◽

Ives Bernardelli de Mattos ◽

...

Keyword(s):

Wound Dressing ◽

Ex Vivo ◽

Cooling Effect ◽

Local Cooling ◽

Bacterial Nanocellulose

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Bacterial Nanocellulose-Enhanced Alginate Double-Network Hydrogels Cross-Linked with Six Metal Cations for Antibacterial Wound Dressing

Polymers ◽

10.3390/polym12112683 ◽

2020 ◽

Vol 12 (11) ◽

pp. 2683

Author(s):

Mina Shahriari-Khalaji ◽

Siyi Hong ◽

Gaoquan Hu ◽

Ying Ji ◽

Feng F. Hong

Keyword(s):

Mechanical Properties ◽

Antibacterial Activity ◽

Wound Dressing ◽

Biomedical Applications ◽

Antibacterial Activities ◽

Ph Responsive ◽

Time Saving ◽

Bacterial Nanocellulose ◽

Vacuum Suction ◽

Copper Zinc

Alginate (Alg) and bacterial nanocellulose (BNC) have exhibited great potential in biomedical applications, especially wound dressing. Non-toxicity and a moisture-maintaining nature are common features making them favorable for functional dressing fabrication. BNC is a natural biopolymer that promotes major advances to the current and future biomedical materials, especially in a flat or tubular membrane form with excellent mechanical strength at hydrated state. The main drawback limiting wide applications of both BNC and Alg is the lack of antibacterial activity, furthermore, the inherent poor mechanical property of Alg leads to the requirement of a secondary dressing in clinical treatment. To fabricate composite dressings with antibacterial activity and better mechanical properties, sodium alginate was efficiently incorporated into the BNC matrix using a time-saving vacuum suction method followed by cross-linking through immersion in separate solutions of six cations (manganese, cobalt, copper, zinc, silver, and cerium). The results showed the fabricated composites had not only pH-responsive antibacterial activities but also improved mechanical properties, which are capable of acting as smart dressings. All composites showed non-toxicity toward fibroblast cells. Rat model evaluation showed the skin wounds covered by the dressings healed faster than by BNC.

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