A New C13-Norisoprenoid from the Fruits of Chaenomeles sinensis

A new megastigmane-type norsesquiterpenoid glycoside, chaemeloside (1), was isolated from the twigs of Chaenomeles sinensis together with 11 known phytochemicals through chromatographic methods. The chemical structure of the new isolate 1 was determined by conventional 1D and 2D NMR data analysis, ECD experiment, hydrolysis followed by a modified Mosher’s method, and LC–MS analysis. The characterized compounds’ biological effects including cytotoxicity against cancer cell lines, antineuroinflammatory activity, and potential neurotrophic effect were evaluated.

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Production of quince nursery trees by different grafting methods

Ciência Rural ◽

10.1590/s0103-84782010000500008 ◽

2010 ◽

Vol 40 (5) ◽

pp. 1049-1052 ◽

Cited By ~ 1

Author(s):

Rafael Pio ◽

Edvan Alves Chagas ◽

Wilson Barbosa ◽

Maria Luiza Sant'anna Tucci ◽

Francisco de Assis Alves Mourão Filho ◽

...

Keyword(s):

Survival Rate ◽

Plant Growth ◽

Graft Survival ◽

Graft Survival Rate ◽

Cydonia Oblonga ◽

Plant Vigor ◽

Plastic Bags ◽

Chaenomeles Sinensis ◽

Nursery Trees ◽

Grafting Methods

Cutting propagation was the system to produce quince nursery trees (Cydonia oblonga). Experiments have been carried out in order to identify news propagations methods. As a result of this research, the 'Japonês' quince (Chaenomeles sinensis) was selected with rootstock, due to its good plant vigor for grafting, rusticity, and adequate performance in the field, mainly in the first years after planting. However, the best grafting period and grafting method must be determined for this rootstock. This research evaluated three grafting methods and five quince scion cultivars on 'Japonês' rootstock. The quince cultivars 'Provence', 'Mendoza Inta-37', 'Portugal', 'Smyrna', and 'Japonês' were grafted by winter cleft grafting, winter budding, and summer budding on 90cm long plants of 'Japonês' quince rootstock seedlings, cultivated in 3-liter plastic bags. Plant growth evaluations started as early as 60 days, and were concluded 150 days after grafting. Cleft grafting resulted in the highest graft survival rate for 'Smyrna', 'Mendoza Inta-37' and 'Japonês' cultivars. Although the higher graft survival rate was recorded in the winter, the buds grafted in the summer had better development.

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Optimization of microwave-assisted extraction followed by RP-HPLC for the simultaneous determination of oleanolic acid and ursolic acid in the fruits of Chaenomeles sinensis

Journal of Separation Science ◽

10.1002/jssc.200900726 ◽

2010 ◽

Vol 33 (8) ◽

pp. 1147-1155 ◽

Cited By ~ 34

Author(s):

Xinsheng Fang ◽

Jianhua Wang ◽

Xiaoliang Yu ◽

Guohui Zhang ◽

Jinpeng Zhao

Keyword(s):

Simultaneous Determination ◽

Ursolic Acid ◽

Oleanolic Acid ◽

Microwave Assisted Extraction ◽

Microwave Assisted ◽

Rp Hplc ◽

Chaenomeles Sinensis ◽

Assisted Extraction

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WS-5 Extract of Curcuma longa, Chaenomeles sinensis, and Zingiber officinale Contains Anti-AChE Compounds and Improves β-Amyloid-Induced Memory Impairment in Mice

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2019/5160293 ◽

2019 ◽

Vol 2019 ◽

pp. 1-16 ◽

Cited By ~ 2

Author(s):

Ju Eun Kim ◽

Abinash Chandra Shrestha ◽

Hyo Shin Kim ◽

Ha Neul Ham ◽

Jun Hyeong Kim ◽

...

Keyword(s):

Memory Impairment ◽

Antioxidant Activities ◽

Curcuma Longa ◽

Senile Plaques ◽

Total Antioxidant Status ◽

Ethanol Extract ◽

Zingiber Officinale ◽

Chaenomeles Sinensis

Alzheimer's disease (AD) is linked to an extensive neuron loss via accumulation of amyloid-beta (Aβ) as senile plaques associated with reactive astrocytes and microglial activation in the brain. The objective of this study was to assess the therapeutic effect of WS-5 ethanol extract in vitro and in vivo against Aβ-induced AD in mice and to identify the extract’s active constituents. In the present study, WS-5 exerted a significant inhibitory effect on acetylcholinesterase (AChE). Analysis by transmission electron microscopy (TEM) revealed that WS-5 prevented Aβ oligomerization via inhibition of Aβ1-42 aggregation. Evaluation of antioxidant activities using 1, 1-diphenyl-2-picrylhydrazyl (DPPH) demonstrated that WS-5 possessed a high antioxidant activity, which was confirmed by measuring the total antioxidant status (TAS). Furthermore, the anti-inflammatory properties of WS-5 were examined using lipopolysaccharide-stimulated BV-2 microglial cells. WS-5 significantly inhibited the lipopolysaccharide–induced production of nitric oxide and two proinflammatory cytokines, TNF-α and IL-6. The memory impairment in mice with Aβ-induced AD was studied using the Morris water maze and passive avoidance test. Immunohistochemistry was performed to monitor pathological changes in the hippocampus and cortex region of the mouse brain. The animal study showed that WS-5 (250 mg/kg) treatment improved learning and suppressed memory impairment as well as reduced Aβ plaque accumulation in Aβ-induced AD. HPLC analysis identified the extract’s active compounds that exert anti-AChE activity. In summary, our findings suggest that WS-5 could be applied as a natural product therapy with a focus on neuroinflammation-related neurodegenerative disorders.

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