High-density genetic map construction and QTL mapping for fiber strength on Chr24 across multiple environments in a CCRI70 recombinant inbred lines population

Euphytica ◽  
2018 ◽  
Vol 214 (6) ◽  
Author(s):  
Xianyan Zou ◽  
Juwu Gong ◽  
Li Duan ◽  
Xiao Jiang ◽  
Zhang Zhen ◽  
...  
BMC Genomics ◽  
2010 ◽  
Vol 11 (1) ◽  
pp. 174 ◽  
Author(s):  
Bernardo Ordas ◽  
Rosa A Malvar ◽  
Rogelio Santiago ◽  
Ana Butron

2019 ◽  
Vol 10 ◽  
Author(s):  
Zhongtang Wang ◽  
Zhong Zhang ◽  
Haixia Tang ◽  
Qiong Zhang ◽  
Guangfang Zhou ◽  
...  

2015 ◽  
Vol 135 (1) ◽  
pp. 124-129 ◽  
Author(s):  
Min Wang ◽  
Shulin Liu ◽  
Shengping Zhang ◽  
Han Miao ◽  
Guili Tian ◽  
...  

2021 ◽  
Author(s):  
Wanli Han ◽  
Jieyin Zhao ◽  
Xiaojuan Deng ◽  
Aixing Gu ◽  
Duolu Li ◽  
...  

Abstract Background: Resistance to Fusarium wilt (FW) is of great significance for increasing the yield of Gossypium barbadense. Most published genetic studies on G. barbadense focus on yield and fiber quality traits, while there are few reports on resistance to FW. Results: To understand the genetic basis of cotton resistance to FW, this study used 110 recombinant inbred lines (RILs) of G. barbadense obtained from the parental materials Xinhai 14 and 06-146, and Nannong was used to construct a high-density genetic linkage map. The high-density genetic map was based on the resequencing of 933,845 single-nucleotide polymorphism (SNP) markers, and 3627 bins covering 2483.17 cM were finally obtained. The collinearity matched the physical map. A total of 9 QTLs for FW resistance were identified, each QTL explained 4.27-14.92% of the observed phenotypic variation, and qFW-Dt3-1 was identified in at least two environments. According to gene annotation information from multiple databases, promoter homeopathic elements and transcriptome data, 10 candidate genes were screened in a stable QTL interval. qRT-PCR analysis showed that the GOBAR_DD06292 gene was differentially expressed in the roots of the two parents under FW stress and exhibited the same expression trend in the G. barbadense resource materials.Conclusions: These results indicate the importance of the GOBAR_DD06292 gene in FW resistance in G. barbadense and lay a molecular foundation for the analysis of the molecular mechanism of FW in G. barbadense.


2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Yuka Miki ◽  
Kentaro Yoshida ◽  
Hiroyuki Enoki ◽  
Shoya Komura ◽  
Kazuyo Suzuki ◽  
...  

AbstractDue to large and complex genomes of Triticeae species, skim sequencing approaches have cost and analytical advantages for detecting genetic markers and building linkage maps. Here, we develop a high-density linkage map and identify quantitative trait loci (QTLs) for recombinant inbred lines of Aegilops tauschii, a D-genome donor of bread wheat, using the recently developed genotyping by Random Amplicon Sequencing-Direct (GRAS-Di) system, which facilitates skimming of the large and complicated genome and generates a large number of genetic markers. The deduced linkage groups based on the GRAS-Di genetic markers corresponded to the chromosome number of Ae. tauschii. We successfully identified stable QTLs for flowering time and spikelet shape-related traits. Genotype differences of RILs at the QTL-linked markers were significantly associated with the trait variations. In particular, one of the QTL-linked markers for flowering time was mapped close to VRN3 (also known as FLOWERING LOCUS T), which controls flowering. The GRAS-Di system is, therefore, an efficient and useful application for genotyping and linkage mapping in species with large and complex genomes, such as Triticeae species.


DNA Research ◽  
2012 ◽  
Vol 19 (4) ◽  
pp. 317-323 ◽  
Author(s):  
N. Sandal ◽  
H. Jin ◽  
D. N. Rodriguez-Navarro ◽  
F. Temprano ◽  
C. Cvitanich ◽  
...  

2019 ◽  
Vol 157 (9-10) ◽  
pp. 659-675 ◽  
Author(s):  
Xiyu Li ◽  
Hong Xue ◽  
Kaixin Zhang ◽  
Wenbin Li ◽  
Yanlong Fang ◽  
...  

AbstractProtein content (PC) and oil content (OC) are important breeding traits of soybean [Glycine max (L.) Merr.]. Quantitative trait locus (QTL) mapping for PC and OC is important for molecular breeding in soybean; however, the negative correlation between PC and OC influences the accuracy of QTL mapping. In the current study, a four-way recombinant inbred lines (FW-RILs) population comprising 160 lines derived from the cross (Kenfeng14 × Kenfeng15) × (Heinong48 × Kenfeng19) was planted in eight different environments and PC and OC measured. Conditional and unconditional QTL analyses were carried out by interval mapping (IM) and inclusive complete IM based on linkage maps of 275 simple sequences repeat markers in a FW-RILs population. This analysis revealed 59 unconditional QTLs and 52 conditional QTLs among the FW-RILs. An analysis of additive effects indicated that the effects of 13 protein QTLs were not related to OC, whereas OC affected the expression of 13 and eight QTLs either partially or completely, respectively. Eight QTLs affecting OC were not influenced by PC, whereas six and 26 QTLs were partially and fully affected by PC, respectively. Among the QTLs detected in the current study, two protein QTLs and five oil QTLs had not been previously reported. These findings will facilitate marker-assisted selection and molecular breeding of soybean.


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