Modulation of key metabolic enzyme of Labeo rohita (Hamilton) juvenile: effect of dietary starch type, protein level and exogenous α-amylase in the diet

2008 ◽  
Vol 35 (2) ◽  
pp. 301-315 ◽  
Author(s):  
Shivendra Kumar ◽  
N. P. Sahu ◽  
A. K. Pal ◽  
Vidya Sagar ◽  
Amit Kumar Sinha ◽  
...  
Keyword(s):  
Protein Level ◽  
Labeo Rohita ◽  
Metabolic Enzyme ◽  
Type Protein ◽  
Dietary Starch

Dietary protein level, microbial phytase, citric acid and their interactions on bone mineralization of Labeo rohita (Hamilton) juveniles

2005 ◽  
Vol 36 (8) ◽  
pp. 803-812 ◽  
Author(s):  
Kartik Baruah ◽  
Asim K Pal ◽  
Narottam P Sahu ◽  
Kamal K Jain ◽  
Subhas C Mukherjee ◽  
...  
Keyword(s):  
Citric Acid ◽  
Dietary Protein ◽  
Protein Level ◽  
Labeo Rohita ◽  
Bone Mineralization ◽  
Dietary Protein Level ◽  
Microbial Phytase

scholarly journals Effect of Squillameal on the Growth and Nutritive Value of Labeo rohita (Ham)

2007 ◽  
Vol 19 (3) ◽  
Author(s):  
L.M. RAO ◽  
K. SATISH KUMAR
Keyword(s):  
Protein Level ◽  
Nutritive Value ◽  
Fish Meal ◽  
Labeo Rohita ◽  
Control Diet ◽  
Fine Powder ◽  
Animal Protein ◽  
Whole Body ◽  
Average Weight ◽  
Groundnut Oil

It has been well established that animal protein performs better than plant protein in the growth and nutritive value of cultivable fish. By far the most suitable animal protein for incorporation into fish diets is fish meal. Fish meal is almost always made from marine fish, and can be based on the by-catches or a product of a specific fishery (De Silva and Anderson, 1995). Squilla species, a marine stomatopod generally called as mantis shrimp, is found in abundance along the Indian coast. The fine powder obtained from grinding the whole body of sun dried squilla species is known as squillameal, which has an excellent nutritive value. Taking this into consideration, it has been proposed to evaluate the suitability of incorporation of squilla meal as animal protein, in the traditional feed (rice bran and groundnut oil cake mixture) of Labeo rohita. For this purpose experiments have been conducted on the fingerlings (of an average weight of 5.33±0.13g) of L. rohita, for 90 days in the laboratory with 35% protein level traditional feed as control diet and 35%and 40% protein level feeds, formulated with squilla meal, as test diets. All the ingredients of these diets have been sieved, steam cooked after through mixing with water, pelletized with the help of a pellet mill and then dried in a hot air oven with blower at about 50o C. The dry pellets of these diets have been used to feed the experimental fingerlings of L. rohita to study the Absolute growth, Specific growth rate (S.G.R), Food conversion ratio (F.C.R), Protein efficiency ratio (P.E.R), and Apparent protein digestibility (A.P.D). The observations revealed that the performance of both the test diets formulated with squilla meal, is far superior to that of the control diet. However, from among the two formulated diets, the one with 35% of animal protein performed better in every respect. Proximate analyses of all the three diets and fish flesh have been carried out to evaluate the relative performance of the two animal protein incorporated artificial diets. The details are dealt in the present paper.

Non-gelatinized corn supplemented with alpha-amylase at sub-optimum protein level enhances the growth of Labeo rohita (Hamilton) fingerlings

2006 ◽  
Vol 37 (3) ◽  
pp. 284-292 ◽  
Author(s):  
Shivendra Kumar ◽  
Narottam P Sahu ◽  
Asim K Pal ◽  
Dharitri Choudhury ◽  
Subhas C Mukherjee
Keyword(s):  
Protein Level ◽  
Labeo Rohita ◽  
Alpha Amylase ◽  
Optimum Protein Level

scholarly journals Inhibition of BUB3 shunts glucose to glycolytic pathway by inducing PFKFB3 accumulation

2019 ◽  
Author(s):  
Jiajin Li ◽  
Ruixue Zhang ◽  
Gang Huang ◽  
Jianjun Liu
Keyword(s):  
Protein Level ◽  
Metabolic Flux ◽  
Mrna Level ◽  
Mitotic Checkpoint ◽  
Glycolytic Enzyme ◽  
Glycolytic Pathway ◽  
Metabolic Reprogramming ◽  
Tumor Promoter ◽  
Biological Behavior ◽  
Metabolic Enzyme

Abstract Purpose: Metabolic reprogramming as a hallmark of cancer has countless connections with other biological behavior of tumor such as rapid mitosis. Mitotic checkpoint protein BUB3 as a key protein involved in the regulation of mitosis is modulated by PKM2, an important glycolytic enzyme. However the role of BUB3 in glucose metabolism remains unknown. Methods: We analyzed the TCGA data to evaluate BUB3 expression in certain tumors. The uptake of glucose and CO2 incorporation was tested by isotopic tracer methods. The lactate, NADPH, NADP and metabolic enzyme activities were tested by assay kits accordingly. Results: We show here that BUB3 is over expressed in cervical cancer and hepatocellular carcinoma. Interference of BUB3 increase the uptake of glucose and shunts the metabolic flux from pentose phosphate pathway to glycolytic pathway. The glycolysis metabolites lactate is increased by BUB3 interference whereas NADPH/NADP ratio is reduced. With regard to metabolic enzymes, interference of BUB3 increase PFKFB3 on protein level and enzyme activity, but not mRNA level. Moreover, the increasing of protein level is diminished when proteasome degradation pathway is blocked by MG132. Conclusions: BUB3 is a potential tumor promoter and plays certain roles in cancer cellular metabolic reprogramming.

scholarly journals Physio-immunological responses of Labeo rohita fingerlings to commonly used phytogenic feed additives: A comparative evaluation

2020 ◽  
Vol 41 (6) ◽  
pp. 1455-1463
Author(s):  
D.K. Chowdhury ◽  
◽  
N.P. Sahu ◽  
P. Sardar ◽  
A.D. Deo ◽  
...  
Keyword(s):  
Antioxidant Enzyme ◽  
Enzyme Activities ◽  
Labeo Rohita ◽  
Black Pepper ◽  
Feed Additives ◽  
Innate Immune ◽  
Metabolic Effects ◽  
Metabolic Enzyme ◽  
Average Weight ◽  
Antioxidant Enzyme Activities

Aim: The present experiment was undertaken to compare digestive, metabolic, antioxidant enzyme activities and immuno-biochemical responses in Labeo rohita fingerlings to some commonly used phytogenic feed additives. Methodology: Eleven experimental diets were prepared by supplementing fennel, coriander, cumin, fenugreek seed, turmeric, black pepper, peeled ginger, bay leaf, peeled onion bulb or peeled garlic clove meal at 1% inclusion level along with a control diet. Four hundred and ninety-five fingerlings (average weight 6.45±0.01 g) were distributed randomly in eleven experimental groups in triplicates with a stocking density of 15 fish per tank (400 l of water). Results: Turmeric, garlic or ginger meals appeared to be more effective than onion, fenugreek, cumin, coriander, fennel, black pepper and bay leaf meals for enhancing digestive, metabolic, antioxidant enzyme activities and innate immune functions. The physio-metabolic effects of phytogenic feed additives tested in Labeo rohita fingerlings were in the order of turmeric > garlic > ginger > onion > fenugreek > cumin > coriander > fennel > black pepper > bay leaf meal. Interpretation: The enhanced digestive and metabolic enzyme activities, antioxidant function, glucose homeostasis and improved innate immune function through modulation of the haemato-biochemical profile of Labeo rohita due to feeding of specific functional compounds present in turmeric, ginger and garlic meals compare to other phytogenic additives.

Dietary optimisation of black gram (Vigna mungo) leaf meal as substitute for deoiled rice bran in the diet of Labeo rohita (Hamilton, 1822) fingerlings

2020 ◽  
Vol 67 (4) ◽  
Author(s):  
Sraddhanjali Sahoo ◽  
Kamal K. Jain ◽  
Narottam P. Sahu ◽  
Ashutosh D. Deo ◽  
N. Shamna ◽  
...  
Keyword(s):  
Rice Bran ◽  
Labeo Rohita ◽  
Vigna Mungo ◽  
The Other ◽  
Metabolic Enzyme ◽  
Control Group ◽  
Black Gram ◽  
Leaf Meal ◽  
Gross Energy ◽  
Metabolic Performance

The study aimed to evaluate the potential of black gram (Vigna mungo) leaf meal (BGLM) as a substitutet for deoiled rice bran (DORB) in the diet of Labeo rohita (Hamilton, 1822) fingerlings. Four isonitrogenous (30% CP) and isocaloric (420 Kcal 100 g-1 gross energy) experimental diets were prepared to conduct the study with different inclusion levels of BGLM viz., control (0 BGLM; 30% DORB), LM10 (10% BGLM, 20% DORB), LM20 (20% BGLM, 10% DORB) and LM30 (30% BGLM, 0% DORB). One hundred forty-four fishes with an initial mean weight of 3.52±0.04 g were arbitrarily assigned to the indoor experimental tubs in triplicates for the 60 days experiment. Fish were fed with experimental diets to satiation twice daily. Percent weight gain and specific growth rate (SGR) were significantly (p<0.05) higher in LM20 group than the other treatment groups (LM10 and LM30) showing no significant (p>0.05) difference with the control group. Among the digestive and metabolic enzyme activities, lipase, hepatic aspartate amino transferase (AST) and alanine amino transferase (ALT) levels were found to be higher in LM20 group compared to the other groups. The study concluded that BGLM can completely replace DORB in the diet of L. rohita. However, the best growth and metabolic performance can be achieved with the inclusion of 20% leaf meal.

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