Research on Responses of Digestive and Metabolic Enzymes to Different Feeding Strategies with Sub-optimal Protein Level in Labeo rohita Fingerlings

2021 ◽  
pp. 55-65
Author(s):  
Pankaj Kumar ◽  
K. K. Jain ◽  
S. Munilkumar
Keyword(s):  
Protein Level ◽  
Labeo Rohita ◽  
Metabolic Enzymes ◽  
Feeding Strategies

Dietary protein level, microbial phytase, citric acid and their interactions on bone mineralization of Labeo rohita (Hamilton) juveniles

2005 ◽  
Vol 36 (8) ◽  
pp. 803-812 ◽  
Author(s):  
Kartik Baruah ◽  
Asim K Pal ◽  
Narottam P Sahu ◽  
Kamal K Jain ◽  
Subhas C Mukherjee ◽  
...  
Keyword(s):  
Citric Acid ◽  
Dietary Protein ◽  
Protein Level ◽  
Labeo Rohita ◽  
Bone Mineralization ◽  
Dietary Protein Level ◽  
Microbial Phytase

scholarly journals Effect of Squillameal on the Growth and Nutritive Value of Labeo rohita (Ham)

2007 ◽  
Vol 19 (3) ◽  
Author(s):  
L.M. RAO ◽  
K. SATISH KUMAR
Keyword(s):  
Protein Level ◽  
Nutritive Value ◽  
Fish Meal ◽  
Labeo Rohita ◽  
Control Diet ◽  
Fine Powder ◽  
Animal Protein ◽  
Whole Body ◽  
Average Weight ◽  
Groundnut Oil

It has been well established that animal protein performs better than plant protein in the growth and nutritive value of cultivable fish. By far the most suitable animal protein for incorporation into fish diets is fish meal. Fish meal is almost always made from marine fish, and can be based on the by-catches or a product of a specific fishery (De Silva and Anderson, 1995). Squilla species, a marine stomatopod generally called as mantis shrimp, is found in abundance along the Indian coast. The fine powder obtained from grinding the whole body of sun dried squilla species is known as squillameal, which has an excellent nutritive value. Taking this into consideration, it has been proposed to evaluate the suitability of incorporation of squilla meal as animal protein, in the traditional feed (rice bran and groundnut oil cake mixture) of Labeo rohita. For this purpose experiments have been conducted on the fingerlings (of an average weight of 5.33±0.13g) of L. rohita, for 90 days in the laboratory with 35% protein level traditional feed as control diet and 35%and 40% protein level feeds, formulated with squilla meal, as test diets. All the ingredients of these diets have been sieved, steam cooked after through mixing with water, pelletized with the help of a pellet mill and then dried in a hot air oven with blower at about 50o C. The dry pellets of these diets have been used to feed the experimental fingerlings of L. rohita to study the Absolute growth, Specific growth rate (S.G.R), Food conversion ratio (F.C.R), Protein efficiency ratio (P.E.R), and Apparent protein digestibility (A.P.D). The observations revealed that the performance of both the test diets formulated with squilla meal, is far superior to that of the control diet. However, from among the two formulated diets, the one with 35% of animal protein performed better in every respect. Proximate analyses of all the three diets and fish flesh have been carried out to evaluate the relative performance of the two animal protein incorporated artificial diets. The details are dealt in the present paper.

scholarly journals Effect of Leaf Aqueous Extract of Manilkara hexandra on Digestive and Glucose Metabolic Enzymes, and Serum Glucose Level in Labeo rohita Fingerlings

2021 ◽  
Author(s):  
Sumana Dutta ◽  
Debnarayan Chowdhury ◽  
Ria Das ◽  
Jayashri Das ◽  
Poulomi Ghosh ◽  
...  
Keyword(s):  
Glucose Level ◽  
Aqueous Extract ◽  
Labeo Rohita ◽  
Metabolic Enzymes ◽  
Serum Glucose ◽  
Serum Glucose Level ◽  
Control Group ◽  
Phosphatase Activities ◽  
Group Iii ◽  
Leaf Aqueous Extract

AbstractManilkara hexandra (Roxb.) Dubard (common name Khirni, family: Sapotaceae) is an evergreen traditionally used medicinal plant. The present study aimed to see the effects of leaf aqueous extract of M. hexandra (LAEMH) on digestive and glucose metabolic enzyme action and serum glucose level in rohu, Labeo rohita, fingerlings. Experimental fish were fed a basal diet (Group I), and diets supplemented with LAEMH at 300 mg (Group II) and 600 mg (Group III) kg−1 body weight. A significant reduction in serum glucose was noticed in the treated groups when compared with the control group. The reduced amylase activity was noticed in the treated fingerlings in a dose-dependent manner. However, lipase and protease activities didn’t differ significantly among the experimental groups. Reduced serum glucose level might be correlated with the decline in the activity of digestive amylase in fish. Group II (3.35±0.19U) and Group III (3.49±0.13 U) were recorded with reduced glucose-6-phosphatase activities than the control group (4.4±0.39 U). Moreover, the study revealed a decline in fructose-1,6-phosphatase activities in the treated groups in comparison to the control group. The decline in the activities of the metabolic enzymes might be associated with the non-availability of glucose owing to reduced activity of the digestive amylase in the treated groups. In conclusion, the present study established the hypoglycaemic effect of the leaf aqueous extract of M. hexandra in a fish model.

Non-gelatinized corn supplemented with alpha-amylase at sub-optimum protein level enhances the growth of Labeo rohita (Hamilton) fingerlings

2006 ◽  
Vol 37 (3) ◽  
pp. 284-292 ◽  
Author(s):  
Shivendra Kumar ◽  
Narottam P Sahu ◽  
Asim K Pal ◽  
Dharitri Choudhury ◽  
Subhas C Mukherjee
Keyword(s):  
Protein Level ◽  
Labeo Rohita ◽  
Alpha Amylase ◽  
Optimum Protein Level

Modulation of key metabolic enzyme of Labeo rohita (Hamilton) juvenile: effect of dietary starch type, protein level and exogenous α-amylase in the diet

2008 ◽  
Vol 35 (2) ◽  
pp. 301-315 ◽  
Author(s):  
Shivendra Kumar ◽  
N. P. Sahu ◽  
A. K. Pal ◽  
Vidya Sagar ◽  
Amit Kumar Sinha ◽  
...  
Keyword(s):  
Protein Level ◽  
Labeo Rohita ◽  
Metabolic Enzyme ◽  
Type Protein ◽  
Dietary Starch

scholarly journals 197 Body temperature of pigs exposed to heat stress conditions: effect of dietary protein level

2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 111-112
Author(s):  
Adriana Morales ◽  
Miguel J Cervantes ◽  
Alan Valle-Fimbres ◽  
Duckens Antoine ◽  
Ernesto Avelar ◽  
...  
Keyword(s):  
Heat Stress ◽  
Body Temperature ◽  
Protein Content ◽  
Dietary Protein ◽  
Protein Level ◽  
Thermal Load ◽  
Feeding Strategies ◽  
Dietary Protein Level ◽  
Amino Acid Intake ◽  
Body Heat

Abstract Heat stress (HS) reduces feed intake and increases body temperature (BT) in pigs. Increased dietary protein content may correct HS-related reduced amino acid intake, but it may further increase BT. The effect of dietary protein level on BT of HS pigs was analyzed with 9-ileal cannulated pigs (61.7 ± 2.7 kg BW). A thermometer set to register BT at 5-min intervals was implanted into the ileum. There were two treatments: low-protein (10.8%) wheat-free Lys-Thr diet (LP); high-protein (21.6%), wheat-soybean meal diet. The study was conducted in two-10 d periods; d1-d7 for diet adaptation and d8 to d10 for BT data analysis. Pigs were fed at 0600 (morning), 1400 (midday), and 2200-h (evening), same amount each time. Ambient temperature (AT) and relative humidity ranged from 30.1 to 35.4 °C and from 50 to 84%, respectively. Both AT and BT followed a similar pattern; BT did not differ between LP and HP pigs (P > 0.05). Postprandial midday and evening BT was higher than postprandial morning BT (P < 0.05). The BT increment after the midday meal was larger and longer (P < 0.05) than that after the morning and evening meals. The postprandial morning and evening BT increments were longer in the HP pigs. Apparently, the capacity of pigs to dissipate postprandial body heat depends on the accumulated thermal load received before their meals; the thermal load before the morning meal was lower than that before the evening meal. In conclusion, these data show that the dietary protein level has a small effect on the BT of HS pigs, confirm that the effect of AT on BT of HS pigs is stronger than that of dietary protein content, and suggest that the capacity of pigs to dissipate body heat increases under HS conditions. This information may be useful to design better feeding strategies for pigs exposed to HS conditions.

scholarly journals Daytime Dependence of the Activity of the Rat Brain Pyruvate Dehydrogenase Corresponds to the Mitochondrial Sirtuin 3 Level and Acetylation of Brain Proteins, All Regulated by Thiamine Administration Decreasing Phosphorylation of PDHA Ser293

2021 ◽  
Vol 22 (15) ◽  
pp. 8006
Author(s):  
Vasily A. Aleshin ◽  
Artem V. Artiukhov ◽  
Thilo Kaehne ◽  
Anastasia V. Graf ◽  
Victoria I. Bunik
Keyword(s):  
Rat Brain ◽  
Pyruvate Dehydrogenase ◽  
Protein Level ◽  
Tricarboxylic Acid Cycle ◽  
Metabolic Enzymes ◽  
Strong Positive Correlation ◽  
Protein Acetylation ◽  
Redox Metabolism ◽  
Brain Proteins ◽  
The Brain

Coupling glycolysis and mitochondrial tricarboxylic acid cycle, pyruvate dehydrogenase (PDH) complex (PDHC) is highly responsive to cellular demands through multiple mechanisms, including PDH phosphorylation. PDHC also produces acetyl-CoA for protein acetylation involved in circadian regulation of metabolism. Thiamine (vitamin B1) diphosphate (ThDP) is known to activate PDH as both coenzyme and inhibitor of the PDH inactivating kinases. Molecular mechanisms integrating the function of thiamine-dependent PDHC into general redox metabolism, underlie physiological fitness of a cell or an organism. Here, we characterize the daytime- and thiamine-dependent changes in the rat brain PDHC function, expression and phosphorylation, assessing their impact on protein acetylation and metabolic regulation. Morning administration of thiamine significantly downregulates both the PDH phosphorylation at Ser293 and SIRT3 protein level, the effects not observed upon the evening administration. This action of thiamine nullifies the daytime-dependent changes in the brain PDHC activity and mitochondrial acetylation, inducing diurnal difference in the cytosolic acetylation and acetylation of total brain proteins. Screening the daytime dependence of central metabolic enzymes and proteins of thiol/disulfide metabolism reveals that thiamine also cancels daily changes in the malate dehydrogenase activity, opposite to those of the PDHC activity. Correlation analysis indicates that thiamine abrogates the strong positive correlation between the total acetylation of the brain proteins and PDHC function. Simultaneously, thiamine heightens interplay between the expression of PDHC components and total acetylation or SIRT2 protein level. These thiamine effects on the brain acetylation system change metabolic impact of acetylation. The changes are exemplified by the thiamine enhancement of the SIRT2 correlations with metabolic enzymes and proteins of thiol-disulfide metabolism. Thus, we show the daytime- and thiamine-dependent changes in the function and phosphorylation of brain PDHC, contributing to regulation of the brain acetylation system and redox metabolism. The daytime-dependent action of thiamine on PDHC and SIRT3 may be of therapeutic significance in correcting perturbed diurnal regulation.

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