Identification, characterization of selenoprotein W and its mRNA expression patterns in response to somatostatin 14, cysteamine hydrochloride, 17β-estradiol and a binary mixture of 17β-estradiol and cysteamine hydrochloride in topmouth culter (Erythroculter ilishaeformis)

Inhibitors of viral disassembly or RNA and protein synthesis, viral disassembly intermediates (infectious subviral particles, ISVP), binary ethylenimine-inactivated virions, and viral particles lacking genomic double-stranded (ds) RNA (empty particles) were used to assess the expression of interleukin-1β (IL-1β) mRNA in chicken (chIL-1β) macrophages in response to avian reovirus. The results demonstrate that two distinct expression patterns of chIL-1β mRNA mediated by different steps in viral replication were found. Viral disassembly was required for the induction of a rapid, transient expression pattern of chIL-1β mRNA that was rapidly induced at 30 min, with maximal levels reached by 2 h, and fell to a low level within 6 h post-inoculation, while viral RNA synthesis rather than protein translation, which was subsequent to membrane penetration, was required to induce a stable, sustained expression pattern of chIL-1β mRNA that occurred at and after 6 h post-inoculation. In addition, the induction of chIL-1β mRNA expression by the empty particles and ISVP was extremely weak, compared with the active dsRNA+ virions or binary ethylenimine-inactivated virions, suggesting that the presence of dsRNA, even if transcriptionally inactive, may be an important factor in this response.

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Molecular cloning and sequence analysis of selenoprotein W gene and its mRNA expression patterns in response to metabolic status and cadmium exposure in goldfish, Carassius auratus

Comparative Biochemistry and Physiology Part B Biochemistry and Molecular Biology ◽

10.1016/j.cbpb.2015.01.005 ◽

2015 ◽

Vol 184 ◽

pp. 1-9 ◽

Cited By ~ 6

Author(s):

Wenbo Chen ◽

Zhen Zhang ◽

Haiyan Dong ◽

Xiaoxue Jiang

Keyword(s):

Sequence Analysis ◽

Mrna Expression ◽

Molecular Cloning ◽

Carassius Auratus ◽

Expression Patterns ◽

Cadmium Exposure ◽

Metabolic Status ◽

Selenoprotein W ◽

Goldfish Carassius Auratus

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Characterization of Ovarian Responses to Equine Chorionic Gonadotropin of Aromatase-Deficient Mice With or Without 17β-Estradiol Supplementation

Endocrinology ◽

10.1210/en.2015-1701 ◽

2016 ◽

Vol 157 (5) ◽

pp. 2093-2103 ◽

Cited By ~ 1

Author(s):

Katsumi Toda ◽

Yoshihiro Hayashi ◽

Masafumi Ono ◽

Toshiji Saibara

Keyword(s):

Mrna Expression ◽

Chorionic Gonadotropin ◽

Reproductive Function ◽

Pcr Analysis ◽

Detailed Knowledge ◽

17Β Estradiol ◽

Equine Chorionic Gonadotropin ◽

Underlying Mechanisms

Abstract Aromatase is an enzyme catalyzing the final step of 17β-estradiol (E2) biosynthesis. Aromatase-deficient (ArKO) mice displayed vital roles of E2 at various tissue sites, including ovary. Here, we report attenuated responses of ArKO ovary to equine chorionic gonadotropin (eCG), an alternative to FSH. Ovarian contents of cAMP and anti-Müllerian hormone (AMH), putative factors reducing sensitivity to gonadotropins, were significantly elevated in ArKO mice compared with those in wild type (WT) mice in the basal state. Accordingly, eCG-induced ovarian alterations in cAMP contents, phosphorylation levels of signaling molecules, and mRNA expression of eCG-targeted genes were blunted in ArKO mice compared with those in WT mice. Treatment of ArKO mice with E2 decreased ovarian cAMP and AMH contents to the WT levels but did not restore the sensitivity. Microarray analysis coupled with quantitative RT-PCR analysis identified 7 genes of which the mRNA expression levels in ArKO ovaries were significantly different from those in the WT ovaries in the basal state and were not normalized by E2 supplementation, indicating possible involvement of these gene products in the determination of ovarian sensitivity to eCG. Thus, present analyses revealed that estrogen deficiency attenuates sensitivity of the ovary to gonadotropin, which might be associated with alterations in the ovarian contents of multiple molecules including cAMP and AMH. Given the importance of the ovarian responses to gonadotropins in reproductive function, detailed knowledge about the underlying mechanisms of abnormalities in the ArKO ovary might help to develop potential targets for infertility treatments.

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Molecular cloning, mRNA expression, and characterization of calm×odulin genes in hybrids of Acanthopagrus schlegeli (♀)×Pagrosomus major (♂)

Journal of Fishery Sciences of China ◽

10.3724/sp.j.1118.2017.17027 ◽

2017 ◽

Vol 24 (6) ◽

pp. 1193 ◽

Cited By ~ 1

Author(s):

Shuyin CHEN ◽

Peng LI ◽

Zhiyong ZHANG ◽

Jin XU ◽

Fei ZHU ◽

...

Keyword(s):

Mrna Expression ◽

Molecular Cloning ◽

Acanthopagrus Schlegeli

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Characterization of the SIRT genes and their distinct expression patterns in Manila clams (Ruditapes philippinarum) exposed to air

Meta Gene ◽

10.1016/j.mgene.2021.100892 ◽

2021 ◽

Vol 28 ◽

pp. 100892

Author(s):

Jingtian Wang ◽

He Zhang ◽

Shuang Xu ◽

Ze Liu ◽

Lu Yang ◽

...

Keyword(s):

Expression Patterns ◽

Ruditapes Philippinarum

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Identification and Characterization of Abiotic Stress Responsive CBL-CIPK Family Genes in Medicago

International Journal of Molecular Sciences ◽

10.3390/ijms22094634 ◽

2021 ◽

Vol 22 (9) ◽

pp. 4634

Author(s):

Wenxuan Du ◽

Junfeng Yang ◽

Lin Ma ◽

Qian Su ◽

Yongzhen Pang

Keyword(s):

Abiotic Stress ◽

Abiotic Stresses ◽

Expression Patterns ◽

Interacting Protein ◽

Forage Crop ◽

Cis Acting ◽

Protein Motifs ◽

Plant Signal Transduction ◽

Identification And Characterization

The calcineurin B-like protein (CBL) and CBL-interacting protein kinase (CIPK) play important roles in plant signal transduction and response to abiotic stress. Plants of Medicago genus contain many important forages, and their growth is often affected by a variety of abiotic stresses. However, studies on the CBL and CIPK family member and their function are rare in Medicago. In this study, a total of 23 CBL and 58 CIPK genes were identified from the genome of Medicago sativa as an important forage crop, and Medicaog truncatula as the model plant. Phylogenetic analysis suggested that these CBL and CIPK genes could be classified into five and seven groups, respectively. Moreover, these genes/proteins showed diverse exon-intron organizations, architectures of conserved protein motifs. Many stress-related cis-acting elements were found in their promoter region. In addition, transcriptional analyses showed that these CBL and CIPK genes exhibited distinct expression patterns in various tissues, and in response to drought, salt, and abscisic acid treatments. In particular, the expression levels of MtCIPK2 (MsCIPK3), MtCIPK17 (MsCIPK11), and MtCIPK18 (MsCIPK12) were significantly increased under PEG, NaCl, and ABA treatments. Collectively, our study suggested that CBL and CIPK genes play crucial roles in response to various abiotic stresses in Medicago.

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Ex vivo mRNA expression of toll-like receptors during latent tuberculosis infection

BMC Immunology ◽

10.1186/s12865-021-00400-4 ◽

2021 ◽

Vol 22 (1) ◽

Author(s):

Birhan Alemnew ◽

Soren T. Hoff ◽

Tamrat Abebe ◽

Markos Abebe ◽

Abraham Aseffa ◽

...

Keyword(s):

Mrna Expression ◽

Expression Patterns ◽

Fold Change ◽

Mrna Levels ◽

Healthy Children ◽

Toll Like Receptors ◽

Relative Expression ◽

Latently Infected ◽

Latent Tb ◽

Latent Tb Infection

Abstract Background Understanding immune mechanisms, particularly the role of innate immune markers during latent TB infection remains elusive. The main objective of this study was to evaluate mRNA gene expression patterns of toll-like receptors (TLRs) as correlates of immunity during latent TB infection and further infer their roles as potential diagnostic biomarkers. Methods Messenger RNA (mRNA) levels were analysed in a total of 64 samples collected from apparently healthy children and adolescents latently infected with tuberculosis (n = 32) or non-infected (n = 32). Relative expression in peripheral blood of selected genes encoding TLRs (TLR-1, TLR-2, TLR-4, TLR-6 and TLR-9) was determined with a quantitative real-time polymerase chain reaction (qRT-PCR) using specific primers and florescent labelled probes and a comparative threshold cycle method to define fold change. Data were analysed using Graph-Pad Prism 7.01 for Windows and a p-value less than 0.05 was considered statistically significant. Results An increased mean fold change in the relative expression of TLR-2 and TLR-6 mRNA was observed in LTBI groups relative to non-LTBI groups (p < 0.05), whereas a slight fold decrease was observed for TLR-1 gene. Conclusions An increased mRNA expression of TLR-2 and TLR-6 was observed in latently infected individuals relative to those non-infected, possibly indicating the roles these biomarkers play in sustenance of the steady state interaction between the dormant TB bacilli and host immunity.

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Galectin-3 is modulated in pancreatic cancer cells under hypoxia and nutrient deprivation

Biological Chemistry ◽

10.1515/hsz-2019-0413 ◽

2020 ◽

Vol 401 (10) ◽

pp. 1153-1165 ◽

Cited By ~ 2

Author(s):

Antônio F. da Silva Filho ◽

Lucas B. Tavares ◽

Maira G. R. Pitta ◽

Eduardo I. C. Beltrão ◽

Moacyr J. B. M. Rêgo

Keyword(s):

Pancreatic Cancer ◽

Cell Death ◽

Mrna Expression ◽

Cancer Cells ◽

Ductal Adenocarcinoma ◽

Reverse Transcription Pcr ◽

Pancreatic Cancer Cells ◽

Through Flow ◽

Galectin 3

AbstractPancreatic ductal adenocarcinoma is one of the most aggressive tumors with a microenvironment marked by hypoxia and starvation. Galectin-3 has been evaluated in solid tumors and seems to present both pro/anti-tumor effects. So, this study aims to characterize the expression of Galectin-3 from pancreatic tumor cells and analyze its influence for cell survive and motility in mimetic microenvironment. For this, cell cycle and cell death were accessed through flow cytometry. Characterization of inside and outside Galectin-3 was performed through Real-Time Quantitative Reverse Transcription PCR (qRT-PCR), immunofluorescence, Western blot, and ELISA. Consequences of Galectin-3 extracellular inhibition were investigated using cell death and scratch assays. PANC-1 showed increased Galectin-3 mRNA expression when cultivated in hypoxia for 24 and 48 h. After 24 h in simultaneously hypoxic/deprived incubation, PANC-1 shows increased Galectin-3 protein and secreted levels. For Mia PaCa-2, cultivation in deprivation was determinant for the increasing in Galectin-3 mRNA expression. When cultivated in simultaneously hypoxic/deprived condition, Mia PaCa-2 also presented increasing for the Galectin-3 secreted levels. Treatment of PANC-1 cells with lactose increased the death rate when cells were incubated simultaneously hypoxic/deprived condition. Therefore, it is possible to conclude that the microenvironmental conditions modulate the Galectin-3 expression on the transcriptional and translational levels for pancreatic cancer cells.

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