scholarly journals Serum N-glycan profiles differ for various breast cancer subtypes

2021 ◽  
Author(s):  
Gerda C. M. Vreeker ◽  
Kiki M. H. Vangangelt ◽  
Marco R. Bladergroen ◽  
Simone Nicolardi ◽  
Wilma E. Mesker ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Early Detection ◽  
Cancer Patients ◽  
High Resolution Mass Spectrometry ◽  
Case Control ◽  
Total Serum ◽  
Screening Methods ◽  
Breast Cancer Patients ◽  
Cancer Subtypes ◽  
Control Comparison

AbstractBreast cancer is the most prevalent cancer in women. Early detection of this disease improves survival and therefore population screenings, based on mammography, are performed. However, the sensitivity of this screening modality is not optimal and new screening methods, such as blood tests, are being explored. Most of the analyses that aim for early detection focus on proteins in the bloodstream. In this study, the biomarker potential of total serum N-glycosylation analysis was explored with regard to detection of breast cancer. In an age-matched case-control setup serum protein N-glycan profiles from 145 breast cancer patients were compared to those from 171 healthy individuals. N-glycans were enzymatically released, chemically derivatized to preserve linkage-specificity of sialic acids and characterized by high resolution mass spectrometry. Logistic regression analysis was used to evaluate associations of specific N-glycan structures as well as N-glycosylation traits with breast cancer. In a case-control comparison three associations were found, namely a lower level of a two triantennary glycans and a higher level of one tetraantennary glycan in cancer patients. Of note, various other N-glycomic signatures that had previously been reported were not replicated in the current cohort. It was further evaluated whether the lack of replication of breast cancer N-glycomic signatures could be partly explained by the heterogenous character of the disease since the studies performed so far were based on cohorts that included diverging subtypes in different numbers. It was found that serum N-glycan profiles differed for the various cancer subtypes that were analyzed in this study.

scholarly journals Clinical Significance of Annexin A2 Expression in Breast Cancer Patients

Cancers ◽  
2020 ◽  
Vol 13 (1) ◽  
pp. 2
Author(s):  
Lee D. Gibbs ◽  
Kelsey Mansheim ◽  
Sayantan Maji ◽  
Rajesh Nandy ◽  
Cheryl M. Lewis ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Patients ◽  
Clinical Significance ◽  
Cell Lines ◽  
Breast Cancer Subtypes ◽  
Enzyme Linked Immunosorbent Assay ◽  
Breast Cancer Patients ◽  
Serum Samples ◽  
Cancer Subtypes ◽  
Tumor Tissues

Increasing evidence suggests that AnxA2 contributes to invasion and metastasis of breast cancer. However, the clinical significance of AnxA2 expression in breast cancer has not been reported. The expression of AnxA2 in cell lines, tumor tissues, and serum samples of breast cancer patients were analyzed by immunoblotting, immunohistochemistry, and enzyme-linked immunosorbent assay, respectively. We found that AnxA2 was significantly upregulated in tumor tissues and serum samples of breast cancer patients compared with normal controls. The high expression of serum AnxA2 was significantly associated with tumor grades and poor survival of the breast cancer patients. Based on molecular subtypes, AnxA2 expression was significantly elevated in tumor tissues and serum samples of triple-negative breast cancer (TNBC) patients compared with other breast cancer subtypes. Our analyses on breast cancer cell lines demonstrated that secretion of AnxA2 is associated with its tyrosine 23 (Tyr23) phosphorylation in cells. The expression of non-phosphomimetic mutant of AnxA2 in HCC1395 cells inhibits its secretion from cells compared to wild-type AnxA2, which further suggest that Tyr23 phosphorylation is a critical step for AnxA2 secretion from TNBC cells. Our analysis of AnxA2 phosphorylation in clinical samples further confirmed that the phosphorylation of AnxA2 at Tyr23 was high in tumor tissues of TNBC patients compared to matched adjacent non-tumorigenic breast tissues. Furthermore, we observed that the diagnostic value of serum AnxA2 was significantly high in TNBC compared with other breast cancer subtypes. These findings suggest that serum AnxA2 concentration could be a potential diagnostic biomarker for TNBC patients.

scholarly journals Self-perceived Risk of Breast Cancer and Screening Behaviors of First-degree Female Relatives of Breast Cancer Patients at the Uganda Cancer Institute, Uganda

2021 ◽  
Author(s):  
Provia Ainembabazi ◽  
Derrick Bary Abila ◽  
Grace Manyangwa ◽  
Godwin Anguzu ◽  
Innocent Mutyaba ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Screening ◽  
Early Detection ◽  
Cancer Patients ◽  
Breast Cancer Screening ◽  
Perceived Risk ◽  
Breast Cancer Patients ◽  
Perceived Risks ◽  
First Degree Relatives ◽  
Cancer Breast

Abstract Introduction In Uganda, Breast cancer is the most common cancer in females globally. The majority of the patients present with advanced-stage disease at diagnoses and experience high mortality. This underscores the importance of early detection approaches based on awareness of risk factors and self-perceived risks, and symptoms of the disease to promote adoption of risk reduction behaviors and prompt health-seeking respectively. This study assessed the self-perceived risk of breast cancer, and breast cancer screening behaviours among first-degree female relatives of breast cancer patients in Uganda.Methods This was a cross-sectional study employing quantitative approaches for data collection and analyses. First-degree female relatives of patients attending care at Uganda Cancer Institute were recruited consecutively in the study. A pre-tested coded questionnaire was used to collect data on self-perceived risks, breast cancer risks, and breast cancer screening behaviours. Data were collected between March to October 2019. A modified Poisson regression model was used to evaluate factors associated with self-perceived risk of breast cancer and breast cancer risk awareness.ResultsWe enrolled 296 first-degree female relatives from 197 female breast cancer patients. The median age (IQR) was 33 (26-43) years. A majority (60.1%, 178/296) of the participants had a low self-perceived risk of breast cancer. Breast self-examination (55.7%, 165/296) was the most practiced screening method followed by clinical breast examination (n= 64/296, 21.6%), ultrasound scan of the breast (7.8%, 23/296,), and mammogram (3.7%, 11/296). Women aged 35-44 years had a higher self-perceived risk of breast cancer (adjusted Incident Rate Ratio [aIRR]: 1.75, 95%CI: 1.10-2.80), compared to women aged 18-25 years. ConclusionFirst-degree relatives reported a low self-perceived risk of breast cancer. Breast cancer health education especially targeting younger women should emphasize the increased risk of breast cancer in first-degree relatives of patients with breast cancer. There is a need to increase awareness of breast cancer screening methods and their usefulness in the early detection of breast cancer among all women in Uganda.

scholarly journals PIWI-Like 1 and PIWI-Like 2 Expression in Breast Cancer

Cancers ◽  
2020 ◽  
Vol 12 (10) ◽  
pp. 2742
Author(s):  
Ramona Erber ◽  
Julia Meyer ◽  
Helge Taubert ◽  
Peter A. Fasching ◽  
Sven Wach ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Patients ◽  
Disease Free Survival ◽  
Breast Cancer Subtypes ◽  
Breast Cancer Patients ◽  
Free Survival ◽  
Cancer Subtypes ◽  
Prognostic Effect ◽  
Enhanced Expression ◽  
Disease Free

PIWI-like 1 and PIWI-like 2 play a role in stem cell self-renewal, and enhanced expression has been reported for several tumor entities. However, few studies have investigated PIWI-like 1 and PIWI-like 2 expressions in breast cancer subtypes regarding prognosis. Therefore, we examined protein expression in a large consecutive cohort of breast cancer patients and correlated it to breast cancer subtypes and survival outcome. PIWI-like 1 and PIWI-like 2 expressions were evaluated using immunohistochemistry in a cohort of 894 breast cancer patients, of whom 363 were eligible for further analysis. Percentage and intensity of stained tumor cells were analyzed and an immunoreactive score (IRS) was calculated. The interaction of PIWI-like 1 and PIWI-like 2 showed a prognostic effect on survival. For the combination of high PIWI-like 1 and low PIWI-like 2 expressions, adjusted hazard ratios (HRs) were significantly higher with regard to overall survival (OS) (HR 2.92; 95% confidence interval (CI) 1.24, 6.90), disease-free survival (DFS) (HR 3.27; 95% CI 1.48, 7.20), and distant disease-free survival (DDFS) (HR 7.64; 95% CI 2.35, 24.82). Both proteins were significantly associated with molecular-like and PAM50 subgroups. Combining high PIWI-like 1 and low PIWI-like 2 expressions predicted poorer prognosis and both markers were associated with aggressive molecular subtypes.

scholarly journals Early detection of circulating tumor DNA for the assessment of resistance or relapse to neoadjuvant chemotherapy in breast cancer patients

2017 ◽  
Vol 28 ◽  
pp. vii5
Author(s):  
E. Isnaldi ◽  
L. Ferrando ◽  
A. Garuti ◽  
G. Cirmena ◽  
P. Franceschelli ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Neoadjuvant Chemotherapy ◽  
Early Detection ◽  
Cancer Patients ◽  
Circulating Tumor Dna ◽  
Breast Cancer Patients ◽  
Tumor Dna
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