Evaluation of genetic structure of Korean wild soybean (Glycine soja) based on saponin allele polymorphism

Saponin chemical composition was phenotyped and genotyped, and saponin composition-based geographical genetic diversity and differentiation were evaluated in Chinese wild soybean (Glycine soja Sieb. & Zucc.). Thirty-two phenotypes and 34 genotypes were confirmed from 3805 wild soybean accessions. Eleven phenotypes (AaαK, AaαIK, AaαIJK, AaBcEαJ, AaBcαK, AbEαIJ, AbαK, AbαIK, AbαIJK, AbβHAb and Aβ0) were newly detected. Four genes had frequencies: Sg-1a 78.8% and Sg-1b 21.0% at the Sg-1 locus; Sg-4 30.7% and Sg-6e 13.7% at their respective loci. The north-eastern and southern populations showed high genetic diversity; the Northeast region contained more novel variants (AuAe, A0, A0Bc, αH, αI αJ, αK, and AbβHAb), and the southern populations contained high frequencies of the Sg-4 gene. Gene differentiation (Fst) analysis suggested that Sg-4 and four group-α saponin alleles or genes (Sg-6e, Sg-6h, Sg-6i, Sg-6j) were important factors influencing the genetic structure and differentiation in Chinese wild soybeans. Geographical differentiation was characterised mainly by latitudinal differences, with two primary groups (north and south) based on saponin genes. Chinese wild soybean accessions differed from Japanese and South Korean ones in genetic structure based on saponin composition, the latter two being likely to have spread from southern China in the glacial stages during the last Ice Age.

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Sampling strategy for wild soybean (Glycine soja) populations based on their genetic diversity and fine-scale spatial genetic structure

Frontiers of Biology in China ◽

10.1007/s11515-007-0060-0 ◽

2007 ◽

Vol 2 (4) ◽

pp. 397-402 ◽

Cited By ~ 5

Author(s):

Weiyue Zhu ◽

Taoying Zhou ◽

Ming Zhong ◽

Baorong Lu

Keyword(s):

Genetic Diversity ◽

Genetic Structure ◽

Glycine Soja ◽

Spatial Genetic Structure ◽

Wild Soybean ◽

Sampling Strategy ◽

Fine Scale

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Population genetic structure of Japanese wild soybean (Glycine soja) based on microsatellite variation

Molecular Ecology ◽

10.1111/j.1365-294x.2006.02854.x ◽

2006 ◽

Vol 15 (4) ◽

pp. 959-974 ◽

Cited By ~ 72

Author(s):

Y. KURODA ◽

A. KAGA ◽

N. TOMOOKA ◽

D. A. VAUGHAN

Keyword(s):

Genetic Structure ◽

Population Genetic Structure ◽

Population Genetic ◽

Glycine Soja ◽

Wild Soybean ◽

Microsatellite Variation

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Fine scale genetic structure in a wild soybean (Glycine soja) population and the implications for conservation

New Phytologist ◽

10.1046/j.1469-8137.2003.00824.x ◽

2003 ◽

Vol 159 (2) ◽

pp. 513-519 ◽

Cited By ~ 36

Author(s):

Yan Jin ◽

Tianhua He ◽

Bao-Rong Lu

Keyword(s):

Genetic Structure ◽

Glycine Soja ◽

Wild Soybean ◽

Fine Scale

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A Novel Pinkish-White Flower Color Variant Is Caused by a New Allele of Flower Color Gene W1 in Wild Soybean (Glycine soja)

Agronomy ◽

10.3390/agronomy11051001 ◽

2021 ◽

Vol 11 (5) ◽

pp. 1001

Author(s):

Jagadeesh Sundaramoorthy ◽

Gyu Tae Park ◽

Hyun Jo ◽

Jeong-Dong Lee ◽

Hak Soo Seo ◽

...

Keyword(s):

Amino Acid ◽

Amino Acid Substitution ◽

Functional Activity ◽

Glycine Soja ◽

Wild Soybean ◽

Flower Color ◽

Loss Of Function ◽

Protein Variation ◽

Color Variant ◽

Color Gene

The enzyme flavonoid 3′,5′-hydroxylase (F3′5′H) plays an important role in producing anthocyanin pigments in soybean. Loss of function of the W1 locus encoding F3′5′H always produces white flowers. However, few color variations have been reported in wild soybean. In the present study, we isolated a new color variant of wild soybean accession (IT261811) with pinkish-white flowers. We found that the flower’s pinkish-white color is caused by w1-s3, a single recessive allele of W1. The SNP detected in the mutant caused amino acid substitution (A304S) in a highly conserved SRS4 domain of F3′5′H proteins. On the basis of the results of the protein variation effect analyzer (PROVEAN) tool, we suggest that this mutation may lead to hypofunctional F3′5′H activity rather than non-functional activity, which thereby results in its pinkish-white color.

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Evaluation of seed amino acid content and its correlation network analysis in wild soybean (Glycine soja) germplasm in Japan

Plant Genetic Resources ◽

10.1017/s1479262121000071 ◽

2021 ◽

Vol 19 (1) ◽

pp. 35-43

Author(s):

Awatsaya Chotekajorn ◽

Takuyu Hashiguchi ◽

Masatsugu Hashiguchi ◽

Hidenori Tanaka ◽

Ryo Akashi

Keyword(s):

Amino Acids ◽

Amino Acid ◽

Free Amino Acid ◽

Acid Content ◽

Free Amino ◽

Glycine Soja ◽

Wild Soybean ◽

Amino Acid Content ◽

Soybean Seeds ◽

Free Amino Acid Content

AbstractWild soybean (Glycine soja) is a valuable genetic resource for soybean improvement. Seed composition profiles provide beneficial information for the effective conservation and utilization of wild soybeans. Therefore, this study aimed to assess the variation in free amino acid abundance in the seeds of wild soybean germplasm collected in Japan. The free amino acid content in the seeds from 316 accessions of wild soybean ranged from 0.965 to 5.987 mg/g seed dry weight (DW), representing a 6.2-fold difference. Three amino acids had the highest coefficient of variation (CV): asparagine (1.15), histidine (0.95) and glutamine (0.94). Arginine (0.775 mg/g DW) was the predominant amino acid in wild soybean seeds, whereas the least abundant seed amino acid was glutamine (0.008 mg/g DW). A correlation network revealed significant positive relationships among most amino acids. Wild soybean seeds from different regions of origin had significantly different levels of several amino acids. In addition, a significant correlation between latitude and longitude of the collection sites and the total free amino acid content of seeds was observed. Our study reports diverse phenotypic data on the free amino acid content in seeds of wild soybean resources collected from throughout Japan. This information will be useful in conservation programmes for Japanese wild soybean and for the selection of accessions with favourable characteristics in future legume crop improvement efforts.

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