Carbon and nitrogen recycling during cyanoHABs in dreissenid-invaded and non-invaded US midwestern lakes and reservoirs

AbstractLakes and reservoirs play key roles in global carbon cycling, especially as a carbon sink. Enrichment of nutrients in lakes and reservoirs (eutrophication) and rising global temperatures favors the proliferation of bloom-forming cyanobacteria. Harmful blooms of cyanobacteria (cyanoHABs) alter carbon and nutrient cycling in freshwater ecosystems. Some evidence suggests the introduction or establishment of invasive mussel species (i.e., Dreissena spp.) also favor cyanoHAB formation through selective filter feeding, a process through which they may also impact biogeochemical processes including carbon cycling and sequestration. However, few studies have considered the combined effects of invasive mussels and cyanoHABs on carbon and nitrogen cycling in freshwater ecosystems. Here, we examined microbial community composition and biogeochemical attributes (including carbon and nitrogen stable isotopes) in eutrophic lakes, reservoirs, and rivers in western Ohio, eastern Indiana, and northern Kentucky during the cyanobacterial bloom period of the summer of 2015. Our samples include both sites impacted by invasive mussels and those where invasive mussels have not yet been observed. Based on 16S and 18S rRNA gene sequence analysis, we found that cyanobacterial and algal communities varied across sites and were most closely related to habitat (sediment or water column sample) and site, regardless of the presence of invasive mussels or other environmental factors. However, we did find evidence that invasive mussels may influence both carbon and nitrogen cycling. While the results are based on a single time point sampling, they highlight the interactions of multiple environmental stressors in aquatic ecosystems and the critical need for more temporally intensive studies of carbon and nutrient cycling in bloom- and mussel-impacted waters.

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Soil fertilization affects the abundance and distribution of carbon and nitrogen cycling genes in the maize rhizosphere

AMB Express ◽

10.1186/s13568-021-01182-z ◽

2021 ◽

Vol 11 (1) ◽

Cited By ~ 1

Author(s):

Matthew Chekwube Enebe ◽

Olubukola Oluranti Babalola

Keyword(s):

Nitrogen Cycling ◽

Carbon Cycling ◽

Inorganic Fertilizer ◽

High Dose ◽

Soil Fertilization ◽

Carbon And Nitrogen ◽

Carbon And Nitrogen Cycling ◽

Fertilized Soil ◽

Catabolic Genes ◽

Maize Plants

AbstractSoil microbes perform important functions in nitrogen and carbon cycling in the biosphere. Microbial communities in the rhizosphere enhance plants’ health and promote nutrient turnover and cycling in the soil. In this study, we evaluated the effects of soil fertilization with organic and inorganic fertilizers on the abundances and distribution of carbon and nitrogen cycling genes within the rhizosphere of maize plants. Our result showed that maize plants through rhizosphere effects selected and enriched the same functional genes glnA, gltB, gudB involved in nitrogen cycle as do high compost and low inorganic fertilizer treatments. This observation was significantly different from those of high doses of inorganic fertilizer and low compost manure treated soil. Only alpha amylase encoding genes were selectively enriched by low compost and high inorganic fertilized soil. The other treatments only selected xynB (in Cp8), lacZ (Cp4), bglA, pldB, trpA (N2), uidA (N1) and glgC, vanA (Cn0) carbon cycling genes in the rhizosphere of maize. Also Actinomycetales are selected by high compost, low inorganic fertilizer and control. The control was without any fertilization and the soil was planted with maize. Bacillales are also promoted by low compost and high inorganic fertilizer. This indicated that only microbes capable of tolerating the stress of high dose of inorganic fertilizer will thrive under such condition. Therefore, soil fertilization lowers nitrogen gas emission as seen with the high abundance of nitrogen assimilation genes or microbial anabolic genes, but increases carbon dioxide evolution in the agricultural soil by promoting the abundance of catabolic genes involve in carbon cycling.

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Phototrophic co-cultures from extreme environments: community structure and potential value for fundamental and applied research

10.1101/427211 ◽

2018 ◽

Cited By ~ 1

Author(s):

Claire Shaw ◽

Charles Brooke ◽

Erik Hawley ◽

Morgan P. Connolly ◽

Javier A. Garcia ◽

...

Keyword(s):

Nitrogen Cycling ◽

Pacific Northwest ◽

Applied Research ◽

National Laboratory ◽

Extreme Environments ◽

Pacific Northwest National Laboratory ◽

16S Rrna Gene Sequencing ◽

Rrna Gene ◽

Carbon And Nitrogen ◽

Carbon And Nitrogen Cycling

ABSTRACTCyanobacteria are found in most illuminated environments and are key players in global carbon and nitrogen cycling. Although significant efforts have been made to advance our understanding of this important phylum, still little is known about how members of the cyanobacteria affect and respond to changes in complex biological systems. This lack of knowledge is in part due to our dependence on pure cultures when determining the metabolism and function of a microorganism. In the work presented here we took advantage of the Culture Collection of Microorganisms from Extreme Environments (CCMEE), a collection of more than 1,000 publicly available photosynthetic co-cultures now maintained at the Pacific Northwest National Laboratory. To highlight some of their scientific potential, we selected 26 of these photosynthetic co-cultures from the CCMEE for 16S rRNA gene sequencing. We assessed if samples readily available from the CCMEE could be used to generate new insights into the role of microbial communities in global and local carbon and nitrogen cycling. Results from this work support the existing notion that culture depositories in general hold the potential to advance fundamental and applied research. If collections of co-cultures can be used to infer roles of the individual organisms remains to be seen and requires further investigation.

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Soil fertilization affects the abundance and distribution of carbon and nitrogen cycling genes in the maize rhizosphere

10.21203/rs.3.rs-26191/v1 ◽

2020 ◽

Author(s):

Matthew Chekwube Enebe ◽

Olubukola Oluranti Babalola

Keyword(s):

Nitrogen Cycling ◽

Carbon Cycling ◽

Inorganic Fertilizer ◽

Soil Fertilization ◽

Carbon And Nitrogen ◽

Carbon And Nitrogen Cycling ◽

Fertilized Soil ◽

Maize Plants ◽

And Control ◽

Compost Manure

Abstract Soil microbes performs important functions in nitrogen and carbon cycling in the biosphere. Microbial communities in the rhizosphere enhance plants’ health and promote nutrient turnover and cycling in the soil. In this experimental study, we evaluated the fundamental effects of soil fertilization with organic (compost manure) and inorganic fertilizer on the abundances and distribution of carbon and nitrogen cycling genes within the rhizospheric regions of maize plants. Our result showed that maize plants through rhizosphere effects selected and enriched the same functional genes glnA, gltB, gudB involved in nitrogen cycle as do higher compost and lower inorganic fertilizer treatments. This observation was significantly different from those of higher doses of inorganic fertilizer and lower compost manure treated soil. Only alpha amylase encoding genes were selectively enriched by lower compost and higher inorganic fertilized soil. The other treatments only selected peculiar carbon cycling genes in the rhizosphere of maize. Also Actinomycetales are selected by high compost, low inorganic fertilizer and control while Bacillales are promoted by low compost and higher inorganic fertilizer and this indicated that only microbes capable of tolerating the stress of higher dose of inorganic fertilizer will thrive under such condition. Therefore, soil fertilization lower nitrogen gas emission but increases carbon dioxide evolution in the agricultural soil.

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Soil fertilization affects the abundance and distribution of carbon and nitrogen cycling genes in the maize rhizosphere

10.21203/rs.3.rs-26191/v2 ◽

2021 ◽

Author(s):

Matthew Chekwube Enebe ◽

Olubukola Oluranti Babalola

Keyword(s):

Nitrogen Cycling ◽

Carbon Cycling ◽

Inorganic Fertilizer ◽

High Dose ◽

Soil Fertilization ◽

Carbon And Nitrogen ◽

Carbon And Nitrogen Cycling ◽

Fertilized Soil ◽

Catabolic Genes ◽

Maize Plants

Abstract Soil microbes perform important functions in nitrogen and carbon cycling in the biosphere. Microbial communities in the rhizosphere enhance plants’ health and promote nutrient turnover and cycling in the soil. In this study, we evaluated the effects of soil fertilization with organic and inorganic fertilizers on the abundances and distribution of carbon and nitrogen cycling genes within the rhizosphere of maize plants. Our result showed that maize plants through rhizosphere effects selected and enriched the same functional genes glnA, gltB, gudB involved in nitrogen cycle as do high compost and low inorganic fertilizer treatments. This observation was significantly different from those of high doses of inorganic fertilizer and low compost manure treated soil. Only alpha amylase encoding genes were selectively enriched by low compost and high inorganic fertilized soil. The other treatments only selected xynB (in Cp8), lacZ (Cp4), bglA, pldB, trpA (N2), uidA (N1) and glgC, vanA (Cn0) carbon cycling genes in the rhizosphere of maize. Also Actinomycetales are selected by high compost, low inorganic fertilizer and control. The control was without any fertilization and the soil was planted with maize. Bacillales are also promoted by low compost and high inorganic fertilizer. This indicated that only microbes capable of tolerating the stress of high dose of inorganic fertilizer will thrive under such condition. Therefore, soil fertilization lowers nitrogen gas emission as seen with the high abundance of nitrogen assimilation genes or microbial anabolic genes, but increases carbon dioxide evolution in the agricultural soil by promoting the abundance of catabolic genes involve in carbon cycling.

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