scholarly journals β-Carotene extraction from Dunaliella salina by supercritical CO2

2021 ◽  
Author(s):  
Kristin Ludwig ◽  
Liisa Rihko-Struckmann ◽  
Gordon Brinitzer ◽  
Gerd Unkelbach ◽  
Kai Sundmacher
Keyword(s):  
Supercritical Co2 ◽  
Dunaliella Salina ◽  
Economic Assessment ◽  
Pilot Scale ◽  
Production Costs ◽  
Green Solvent ◽  
Solvent Concentration ◽  
Organic Solvent Extraction ◽  
Potential Alternative ◽  
Β Carotene

AbstractThis paper reports the results of supercritical carbon dioxide (scCO2) extraction of β-carotene from Dunaliella salina as potential alternative to conventional organic solvent extraction. In pilot-scale scCO2 experiments, the pressure, temperature, and co-solvent concentration were varied. The supercritical extraction at 500 bar, 70 °C, and 10 wt% ethanol as co-solvent yielded in the highly efficient pigment recovery of over 90%. Techno-economic assessment demonstrated higher energy consumption for the scCO2 extraction that was compensated by lower solvent costs. Thus, comparable pigment production costs to the reference extraction with n-hexane were estimated for the scCO2 process. Due to the green solvent properties of scCO2 and ethanol, this approach is highly promising for extraction of algal biomass in industrial scale.

scholarly journals Molecular and phylogenetic analysis reveals new diversity of Dunaliella salina from hypersaline environments

2021 ◽  
pp. 1-11
Author(s):  
Andrea Highfield ◽  
Angela Ward ◽  
Richard Pipe ◽  
Declan C. Schroeder
Keyword(s):  
Genetic Diversity ◽  
Phylogenetic Analysis ◽  
Dunaliella Salina ◽  
Phylogenetic Analyses ◽  
Ssu Rdna ◽  
Lsu Rdna ◽  
Hypersaline Environments ◽  
Rdna Its ◽  
Β Carotene ◽  
Selection Of

Abstract Twelve hyper-β carotene-producing strains of algae assigned to the genus Dunaliella salina have been isolated from various hypersaline environments in Israel, South Africa, Namibia and Spain. Intron-sizing of the SSU rDNA and phylogenetic analysis of these isolates were undertaken using four commonly employed markers for genotyping, LSU rDNA, ITS, rbcL and tufA and their application to the study of Dunaliella evaluated. Novel isolates have been identified and phylogenetic analyses have shown the need for clarification on the taxonomy of Dunaliella salina. We propose the division of D. salina into four sub-clades as defined by a robust phylogeny based on the concatenation of four genes. This study further demonstrates the considerable genetic diversity within D. salina and the potential of genetic analyses for aiding in the selection of prospective economically important strains.

Effects of various environmental factors on β-carotene production by Dunaliella salina. —note—

1992 ◽  
Vol 73 (2) ◽  
pp. 178
Author(s):  
Yukiho Yamaoka ◽  
Osamu Takimura ◽  
Hiroyuki Fuse ◽  
Kazuo Kamimura ◽  
Eichi Manabe ◽  
...  
Keyword(s):  
Environmental Factors ◽  
Dunaliella Salina ◽  
Β Carotene

Prospective environmental and economic assessment for biotreatment of micropollutants in drinking water resources in Denmark

2015 ◽  
Vol 15 (6) ◽  
pp. 1405-1413 ◽  
Author(s):  
Ivan Muñoz ◽  
Erik de Vries ◽  
Janneke Wittebol ◽  
Jens Aamand
Keyword(s):  
Drinking Water ◽  
Water Resources ◽  
Economic Assessment ◽  
Pilot Scale ◽  
Impact Categories ◽  
Financial Cost ◽  
Sand Filters ◽  
Immobilized Bacteria ◽  
Drinking Water Resources ◽  
Scale Data

A prospective environmental life cycle assessment (LCA) and financial cost assessment is performed to the application of bioaugmentation to sand filters in Danish waterworks, to remove 2,6-dichlorobenzamide (BAM) from drinking water resources. Based on pilot-scale and laboratory-scale data, we compare bioaugmentation to current alternative strategies, namely granular activated carbon (GAC) adsorption, and well re-location. Both assessments identified well re-location as the least preferred option, however, this result is very sensitive to the distance from the waterworks to the new well. When bioaugmentation is compared to GAC, the former has a lower impact in 13 impact categories, but if immobilized bacteria are used, the impacts are higher than for GAC in all impact categories. On the other hand, from a cost perspective bioaugmentation appears to be preferable to GAC only if immobilized bacteria are used.

Precipitation of β-carotene and PHBV and co-precipitation from SEDS technique using supercritical CO2

2008 ◽  
Vol 47 (2) ◽  
pp. 259-269 ◽  
Author(s):  
Elton Franceschi ◽  
Alana M. De Cesaro ◽  
Mirian Feiten ◽  
Sandra R.S. Ferreira ◽  
Cláudio Dariva ◽  
...  
Keyword(s):  
Supercritical Co2 ◽  
Co Precipitation ◽  
Β Carotene

scholarly journals A Microwave-Based Chemical Factory in the Lab: From Milligram to Multigram Preparations

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Laura Rinaldi ◽  
Diego Carnaroglio ◽  
Laura Rotolo ◽  
Giancarlo Cravotto
Keyword(s):  
Process Intensification ◽  
Pilot Scale ◽  
Production Costs ◽  
Microwave Chemistry ◽  
Reaction Conditions ◽  
Flow Reactors ◽  
Advantages And Disadvantages ◽  
Microwave Reactors ◽  
Flow Through ◽  
Parallel Tests

Microwave technology is changing the way we design and optimize synthetic protocols and their scaling up to multigram production levels. The latest generation of dedicated microwave reactors enables operators to quickly screen reaction conditions by means of parallel tests and select the best catalyst, solvent, and conditions. Pilot scale synthetic procedures require flow-through conditions in microwave flow reactors which can be obtained by adapting classic batch protocols. Microwave-assisted chemical processes play a pivotal role in the design of sustainable multigram preparations which address the double requirement of process intensification and competitive production costs. Although most researchers are likely to be acquainted with the great potential of dielectric heating, the advantages and disadvantages of a particular device or the conditions needed to maximize efficiency and functionality are often overlooked. The double aims of the present review are to provide a panoramic snapshot of commercially available lab microwave reactors and their features as well as highlighting a few selected applications of microwave chemistry of particular relevance.

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