Effects of Mn2+ on neutral lipid content, C4 pathway, and related gene expression in Phaeodactylum tricornutum

AbstractQualitative and quantitative changes in the neutral lipid content of hemolymph of gypsy moths, Lymantria dispar L., were assayed in larval, pupal, and adult stages. The major neutral lipid constituents of the hemolymph were 1,2-diacylglycerols and ranged in nominal concentration from 1.6–3.4 (larval), 3.1–4.9(pupal),toamaximumof 19.3 μg lipid per microlitre hemolymph in the adult male. When detected, triacylglycerols and monoacylglycerols never exceed diacylglycerol concentrations. The fatty acid composition of 1,2-diacylglycerols from adult male moths (0–12 h after emergence) was determined as fatty acid methyl esters using gas chromatography/mass spectrometry analysis. Nine fatty acid structures have been assigned. Of these, five are saturated, unbranched, aliphatic fatty acids (C14:0 – C18:0) which comprise 80.5% of the total fatty acid abundance. The remaining four fatty acids consist of two saturated, methyl-branched, aliphatic compounds, a mono-unsaturated aliphatic acid, and a tri-unsaturated, tricyclic, diterpenoid acid.

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Enhancing neutral lipid content in Skeletonema marinoi through multiple phase growth in a bench photobioreactor

Algal Research ◽

10.1016/j.algal.2014.05.004 ◽

2014 ◽

Vol 5 ◽

pp. 32-36 ◽

Cited By ~ 2

Author(s):

Elena Bertozzini ◽

Luca Galluzzi ◽

Antonella Penna ◽

Mauro Magnani

Keyword(s):

Neutral Lipid ◽

Lipid Content ◽

Phase Growth ◽

Multiple Phase ◽

Neutral Lipid Content ◽

Skeletonema Marinoi

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Starvation-induced lipid reserve depletion in Pratylenchus brachyurus leads to decreased infectivity in maize roots

Nematology ◽

10.1163/15685411-bja10032 ◽

2020 ◽

Vol 23 (1) ◽

pp. 103-111

Author(s):

Paula S. Alves ◽

Willian C. Terra ◽

Giselle B. Pinto ◽

Paulo V.M. Pacheco ◽

Bárbhara J.R. Fatobene ◽

...

Keyword(s):

Neutral Lipid ◽

Lipid Content ◽

Consumption Rate ◽

Neutral Lipids ◽

Control Strategies ◽

Maize Roots ◽

Neutral Lipid Content ◽

Pratylenchus Brachyurus ◽

Waiting Periods ◽

Oil Red O

Summary Nematode body neutral lipid (triacylglycerol) content has been related to infectivity and has direct implications in control strategies. In this study, Pratylenchus brachyurus populations were split into two groups: i) freshly hatched second-stage juveniles (J2) containing lipids stored during embryogenesis; ii) third- and fourth-stage juveniles (J3/J4) plus females that replenished their lipid reserves by feeding on maize (Zea mays) roots. These groups were subjected to starvation to study their lipid consumption dynamics by staining with Oil Red O, which binds specifically to neutral lipids. Before starvation, freshly hatched J2 had 27% of their body area stained, whereas J3/J4 and females had 75%. Freshly hatched J2 starved for 28 days at 25°C in water lost 63.8% of the original neutral lipid content, which caused a reduction of 91% of infectivity in maize roots. By contrast, J3/J4 and females exposed to the same conditions lost 56.7% of the original neutral lipid content, which resulted in less than 50% reduction in infectivity. During the period of food deprivation, J2 had a mean daily neutral lipid consumption rate of 0.63% and the other infectious stages (J3/J4 and females) had a mean daily neutral lipid consumption rate of 1.46% per day. This study adds information on the dynamics of lipid utilisation that supports the use of longer waiting periods for planting crops after fallow in soils infested with P. brachyurus as compared to Meloidogyne spp.-infested soils.

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Abstract B69: Cellular neutral lipid content is a novel biomarker of cancer preventive activity

10.1158/1940-6207.prev-11-b69 ◽

2011 ◽

Author(s):

Ivan P. Uray ◽

Reid Bissonnette ◽

Powel H. Brown

Keyword(s):

Neutral Lipid ◽

Lipid Content ◽

Preventive Activity ◽

Neutral Lipid Content

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Age-related changes in Heligmosomoides polygyrus (Nematoda): neutral lipid content in developing oocytes

Canadian Journal of Zoology ◽

10.1139/z88-407 ◽

1988 ◽

Vol 66 (12) ◽

pp. 2791-2796

Author(s):

Barbara M. MacKinnon ◽

D. L. Lee

Keyword(s):

Neutral Lipid ◽

Lipid Content ◽

Egg Production ◽

Reproductive Tract ◽

Developmental Stages ◽

Significant Loss ◽

Female Reproductive Tract ◽

Heligmosomoides Polygyrus ◽

Age Related ◽

Neutral Lipid Content

Changes in neutral lipid content of developing female gametes in Heligmosomoides polygyrus at 8, 12, 20, 40, 80, and 140 days postinfection (p.i.) were investigated and correlated with egg production by the worms over the same period. Egg production increased to day 20 p.i. when the average egg output for one female reached approximately 700 eggs/day. A decline in egg production occurred from 80 days p.i. until the end of the experiment (140 days p.i.). Neutral lipid content was low in oogonia from worms of all ages. Developing oocytes contained the highest levels of neutral lipid. There was a significant loss of lipid just before fertilization of the oocytes. An increase in lipid occurred in all developmental stages of gametes from day 8 to day 40 p.i., and a significant decline occurred thereafter to day 140 p.i. Although egg production and lipid content of the female reproductive tract showed similar trends, there was not a precise correlation. It is felt that nuclear and cytoplasmic processes other than lipid anabolism or catabolism within the developing gametes play a more important role in influencing egg output.

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Fluorometric determination of the neutral lipid content of microalgal cells using Nile Red

Journal of Microbiological Methods ◽

10.1016/0167-7012(87)90019-4 ◽

1987 ◽

Vol 6 (6) ◽

pp. 333-345 ◽

Cited By ~ 242

Author(s):

Keith E. Cooksey ◽

James B. Guckert ◽

Scott A. Williams ◽

Patrik R. Callis

Keyword(s):

Neutral Lipid ◽

Lipid Content ◽

Nile Red ◽

Fluorometric Determination ◽

Neutral Lipid Content

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The neutral lipid composition and size of recombinant high density lipoproteins regulates lecithin:cholesterol acyltransferase activity

Biochemistry and Cell Biology ◽

10.1139/o89-056 ◽

1989 ◽

Vol 67 (7) ◽

pp. 358-364 ◽

Cited By ~ 11

Author(s):

D. L. Sparks ◽

P. H. Pritchard

Keyword(s):

Cholesteryl Ester ◽

Neutral Lipid ◽

Lipid Content ◽

High Density ◽

High Density Lipoproteins ◽

Surface Lipid ◽

Lecithin:Cholesterol Acyltransferase ◽

Catalytic Potential ◽

Neutral Lipid Content ◽

Triacylglycerol Content

Recombinant high density lipoprotein (rHDL) particles were prepared from purified lipids and human apoproteins, and the ability of these complexes to act as substrates for purified lecithin:cholesterol acyltransferase (LCAT) was determined. Increasing the triacylglycerol content relative to cholesteryl ester in rHDL markedly decreased the maximum catalytic potential of LCAT. Kinetic analysis showed that the Vmax of the LCAT reaction was significantly and negatively correlated to the triacylglycerol content. The apparent Km was not directly affected by relative neutral lipid content, but was significantly related to protein and surface lipid content as well as to particle size. These results suggest that while particulate size may regulate the interaction between LCAT and HDL, the relative neutral lipid content of the particle may play a major role in regulating the catalytic potential of the enzyme, particularly with HDL from hypertriglyceridemic patients.Key words: high density lipoproteins, lecithin:cholesterol acyltransferase, model lipoproteins, triacylglycerol, cholesteryl ester.

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