Scorzonera calyculata Aerial Part Extract Mediated Synthesis of Silver Nanoparticles: Evaluation of Their Antibacterial, Antioxidant and Anticancer Activities

2019 ◽  
Vol 30 (4) ◽  
pp. 1037-1050 ◽  
Author(s):  
Anahita Ayromlou ◽  
Shiva Masoudi ◽  
Amir Mirzaie
Keyword(s):  
Silver Nanoparticles ◽  
Aerial Part ◽  
Anticancer Activities

scholarly journals Biofabricated Fatty Acids-Capped Silver Nanoparticles as Potential Antibacterial, Antifungal, Antibiofilm and Anticancer Agents

2021 ◽  
Vol 14 (2) ◽  
pp. 139
Author(s):  
Mohammad Azam Ansari ◽  
Sarah Mousa Maadi Asiri ◽  
Mohammad A. Alzohairy ◽  
Mohammad N. Alomary ◽  
Ahmad Almatroudi ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Silver Nanoparticles ◽  
Anticancer Agents ◽  
Sem Analysis ◽  
Dependent Manner ◽  
Anticancer Activities ◽  
Anticancer Efficacy ◽  
Hct 116 ◽  
Hct 116 Cells ◽  
Dose Dependent

The current study demonstrates the synthesis of fatty acids (FAs) capped silver nanoparticles (AgNPs) using aqueous poly-herbal drug Liv52 extract (PLE) as a reducing, dispersing and stabilizing agent. The NPs were characterized by various techniques and used to investigate their potent antibacterial, antibiofilm, antifungal and anticancer activities. GC-MS analysis of PLE shows a total of 37 peaks for a variety of bio-actives compounds. Amongst them, n-hexadecanoic acid (21.95%), linoleic acid (20.45%), oleic acid (18.01%) and stearic acid (13.99%) were found predominately and most likely acted as reducing, stabilizing and encapsulation FAs in LIV-AgNPs formation. FTIR analysis of LIV-AgNPs shows some other functional bio-actives like proteins, sugars and alkenes in the soft PLE corona. The zone of inhibition was 10.0 ± 2.2–18.5 ± 1.0 mm, 10.5 ± 2.5–22.5 ± 1.5 mm and 13.7 ± 1.0–16.5 ± 1.2 against P. aeruginosa, S. aureus and C. albicans, respectively. LIV-AgNPs inhibit biofilm formation in a dose-dependent manner i.e., 54.4 ± 3.1%—10.12 ± 2.3% (S. aureus), 72.7 ± 2.2%–23.3 ± 5.2% (P. aeruginosa) and 85.4 ± 3.3%–25.6 ± 2.2% (C. albicans), and SEM analysis of treated planktonic cells and their biofilm biomass validated the fitness of LIV-AgNPs in future nanoantibiotics. In addition, as prepared FAs rich PLE capped AgNPs have also exhibited significant (p < 0.05 *) antiproliferative activity against cultured HCT-116 cells. Overall, this is a very first demonstration on employment of FAs rich PLE for the synthesis of highly dispersible, stable and uniform sized AgNPs and their antibacterial, antifungal, antibiofilm and anticancer efficacy.

Annona muricata silver nanoparticles exhibit strong anticancer activities against cervical and prostate adenocarcinomas through regulation of CASP9 and the CXCL1/CXCR2 genes axis

Tumor Biology ◽  
2021 ◽  
Vol 43 (1) ◽  
pp. 37-55
Author(s):  
Yahaya Gavamukulya ◽  
Esther N. Maina ◽  
Hany A. El-Shemy ◽  
Amos M. Meroka ◽  
Geoffrey K. Kangogo ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Anticancer Drugs ◽  
Hela Cells ◽  
Selectivity Index ◽  
Annona Muricata ◽  
Anticancer Activities ◽  
Apoptosis Pathway ◽  
Migration Assay ◽  
Intrinsic Apoptosis Pathway ◽  
In Cells

BACKGROUND: Green synthesized nanoparticles have been earmarked for use in nanomedicine including for the development of better anticancer drugs. OBJECTIVE: The aim of this study was to undertake biochemical evaluation of anticancer activities of green synthesized silver nanoparticles (AgNPs) from ethanolic extracts of fruits (AgNPs-F) and leaves (AgNPs-L) of Annona muricata. METHODS: Previously synthesized silver nanoparticles were used for the study. The effects of the AgNPs and 5-Fluorouracil were studied on PC3, HeLa and PNT1A cells. The resazurin, migration and colonogenic assays as well as qRT-PCR were employed. RESULTS: The AgNPs-F displayed significant antiproliferative effects against HeLa cells with an IC50 of 38.58μg/ml and PC3 cells with an IC50 of 48.17μg/ml but selectively spared normal PNT1A cells (selectivity index of 7.8), in comparison with first line drug 5FU and AgNPs-L whose selectivity index were 3.56 and 2.26 respectively. The migration assay revealed potential inhibition of the metastatic activity of the cells by the AgNPs-F while the colonogenic assay indicated the permanent effect of the AgNPs-F on the cancer cells yet being reversible on the normal cells in contrast with 5FU and AgNPs-L. CASP9 was significantly over expressed in all HeLa cells treated with the AgNPs-F (1.53-fold), AgNPs-L (1.52-fold) and 5FU (4.30-fold). CXCL1 was under expressed in HeLa cells treated with AgNPs-F (0.69-fold) and AgNPs-L (0.58-fold) and over expressed in cells treated with 5FU (4.95-fold), but the difference was not statistically significant. CXCR2 was significantly over expressed in HeLa cells treated with 5FU (8.66-fold) and AgNPs-F (1.12-fold) but under expressed in cells treated with AgNPs-L (0.76-fold). CONCLUSIONS: Here we show that biosynthesized AgNPs especially AgNPs-F can be used in the development of novel and better anticancer drugs. The mechanism of action of the AgNPs involves activation of the intrinsic apoptosis pathway through upregulation of CASP9 and concerted down regulation of the CXCL1/ CXCR2 gene axis.

scholarly journals Phytosynthesis of silver nanoparticles using Artemisia marschalliana Sprengel aerial part extract and assessment of their antioxidant, anticancer, and antibacterial properties

2016 ◽  
pp. 1835 ◽  
Author(s):  
Mehdi Shafiee Ardestani ◽  
Seyed Ataollah Sadat Shandiz ◽  
Soheil Salehi ◽  
Farinaz Ghanbar ◽  
Mohammad Raouf Darvish ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Aerial Part ◽  
Antibacterial Properties

scholarly journals Green Biosynthesis, Antioxidant, Antibacterial, and Anticancer Activities of Silver Nanoparticles of Luffa acutangula Leaf Extract

2021 ◽  
Vol 2021 ◽  
pp. 1-28
Author(s):  
Devi Nallappan ◽  
Agustine Nengsih Fauzi ◽  
Balam Satheesh Krishna ◽  
Basivi Praveen Kumar ◽  
Avula Vijaya Kumar Reddy ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Aspartic Acid ◽  
Attenuated Total Reflection ◽  
Engineered Nanoparticles ◽  
Face Centered Cubic ◽  
Research Strategies ◽  
Anticancer Activities ◽  
Drug Candidates ◽  
Luffa Acutangula ◽  
Mcf 7

Studies on green biosynthesis of newly engineered nanoparticles for their prominent medicinal applications are being the torch-bearing concerns of the state-of-the-art research strategies. In this concern, we have engineered the biosynthesized Luffa acutangula silver nanoparticles of flavonoid O-glycosides in the anisotropic form isolated from aqueous leave extracts of Luffa acutangula, a popular traditional and ayurvedic plant in south-east Asian countries. These were structurally confirmed by Ultraviolet-visible (UV-Vis), Fourier transform infrared spectroscopy accessed with attenuated total reflection (FTIR-ATR) spectral analyses followed by the scanning electron microscopic (SEM) and the X-ray diffraction (XRD) crystallographic studies and found them with the face-centered cubic (fcc) structure. Medicinally, we have explored their significant antioxidant (DPPH and ABTS assays), antibacterial (disc diffusion assay on E. coli, S. aureus, B. subtilis, S. fecilis, and S. boydii), and anticancer (MTT assay on MCF-7, MDA-MB-231, U87, and DBTRG cell lines) potentialities which augmented the present investigation. The molecular docking analysis of title compounds against 3NM8 (DPPH) and 1DNU (ABTS) proteins for antioxidant activity; 5FGK (Gram-Positive Bacteria) and 1AB4 (Gram-Negative Bacteria) proteins for antibacterial activity; and 4GBD (MCF-7), 5FI2 (MDA-MB-231), 1D5R (U87), and 5TIJ (DBTRG) proteins for anticancer activity has affirmed the promising ligand-protein binding interactions among the hydroxy groups of the title compounds and aspartic acid of the concerned enzymatic proteins. The binding energy varying from -9.1645 to -7.7955 for Cosmosioside (1, Apigenin-7-glucoside) and from -9.2690 to -7.8306 for Cynaroside (2, Luteolin-7-glucoside) implies the isolated compounds as potential bioactive compounds. In addition, the performed studies like QSAR, ADMET, bioactivity properties, drug scores, and toxicity risks confirmed them as potential drug candidates and aspartic acid receptor antagonists. This research auxiliary augmented the existing array of phytological nanomedicines with new drug candidates that are credible with multiple bioactivities.

scholarly journals Saponin Composition Analysis of the Aerial Part of Panax Bipinnatifidus Seem. Collected in Sa Pa, Lao Cai

2019 ◽  
Vol 35 (1) ◽  
Author(s):  
Dang Thi Ngan ◽  
Bui Thi Thanh Van ◽  
Tran Thi Ngoc Ha ◽  
Nong My Hoa ◽  
Cao Thi Phuong Thao ◽  
...  
Keyword(s):  
Spectroscopic Data ◽  
Aerial Part ◽  
Chemical Diversity ◽  
Composition Analysis ◽  
Viet Nam ◽  
Anticancer Activities ◽  
Pentacyclic Triterpenoids ◽  
Chromatographic Techniques ◽  
Lao Cai ◽  
Stem Leaf

Panax bipinnatifidus Seem. is a precious medicinal plant belonging to the Araliaceae family. This study qualitatively analyzed saponins of the stem, leaf and rhizome of P. bipinnatifidus by HPLC. Subsequently, by using chromatographic techniques, a major saponin from the leaf of P.bipinnatifidius Seem. was isolated. On the basis of NMR and MS spectroscopic data as well as comparison with those reported in the literature, the isolated saponin’s structure was identified as stipuleanoside R2. To the best our knowledge, this is the first report of saponin from the aerial part of P. bipinnatifidius Seem. Keywords Panax bipinnatifidus, Araliaceae, Stipuleanosid R2, HPLC.. [1] Nguyễn Văn Tập, Các loài thuộc chi Panax L. ở Việt Nam, Tạp chí Dược liệu. 10(3) (2005) 71-76. [2] Nguyễn Văn Tập, Phạm Thanh Huyền, Kết quả nghiên cứu về phân bố, sinh thái sâm vũ diệp và Tam thất hoang ở Việt Nam, Tạp chí Dược liệu. 11(5) (2006) 177-180. [3] Nguyen Huu Tung, Tran Hong Quang, Nguyen Thị Thanh Ngan, Chau Van Minh, Bui Kim Anh, Pham Quoc Long, Nguyen Manh Cuong, Young Ho Kim, Oleanolic triterpenesaponins from the roots of Panax bipinnatifidus, Chem Pharm Bull (Tokyo). 59(11) (2011) 1417-1420. [4] Đỗ Văn Hào, Nguyễn Thị Huệ, Nguyễn Thị Thu Thủy, Đặng Thị Ngần, Đào Thị Hồng Bích, Nguyễn Thị Hoàng Anh, Dương Thị Ly Hương, Nguyễn Hữu Tùng, Thành phần hóa học của phân đoạn ethyl acetat từ rễ cây sâm vũ diệp (Panax bipinnatifidus Seem.) thu hái ở Sa Pa, Lào Cai, Tạp chí Khoa học ĐHQGHN - Khoa học Y Dược. 33(2) (2017) 50-55. [5] Nguyễn Thị Thu Thủy, Nguyễn Thị Huệ, Đặng Thị Thùy, Nguyễn Thị Hoàng Anh, Dương Thị Phượng, Phạm Thị Tuyết Nhung, Hà Vân Oanh, Dương Thị Ly Hương, Nguyễn Hữu Tùng, Thành phần saponin của thân rễ sâm vũ diệp thu hái ở Sa Pa, Lào Cai, Tạp chí Dược liệu. 23(2) (2018) 82-88.[6] Wen-zhi Yang, Ying Hu, Wan-ying Wu, Min Ye, De-an Guo, Saponins in the genus Panax L. (Araliaceae): A systematic review of their chemical diversity, Phytochemistry. 106 (2014) 7-14. [7] Shashi B. Mahato, Asish P. Kundu, 13C NMR spectra of pentacyclic triterpenoids - a complication and some salient features, Phytochemistry. 37 (1994) 1517-1575. [8] Pawan K. Agrawal, NMR spectroscopy in the structural elucidation of oligossacharides and glycosides, Phytochemistry. 31 (1992) 1307-1330. [9] Chun Liang, Yan Ding, Huu Tung Nguyen, Jeong-Ah Kim, Hye-Jin Boo, Hee-Kyoung Kang, Mahn Cuong Nguyen, Young Ho Kim, Oleanane –type triterpenoids from Panax stipuleannatus and their anticancer activities, Bioorg Med Chem Lett. 20 (2010) 7110-7115.  

scholarly journals Biosynthesis and Characterization of Extracellular Silver Nanoparticles from Streptomyces aizuneusis: Antimicrobial, Anti Larval, and Anticancer Activities

Molecules ◽  
2021 ◽  
Vol 27 (1) ◽  
pp. 212
Author(s):  
Hemmat M. Abd-Elhady ◽  
Mona A. Ashor ◽  
Abdelkader Hazem ◽  
Fayez M. Saleh ◽  
Samy Selim ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Human Lung ◽  
Spherical Shape ◽  
Antimicrobial Activities ◽  
Anticancer Activities ◽  
X Ray ◽  
Transmission Electron ◽  
Vis Spectroscopy ◽  
Human Lung Carcinoma

The ability of microorganisms to reduce inorganic metals has launched an exciting eco-friendly approach towards developing green nanotechnology. Thus, the synthesis of metal nanoparticles through a biological approach is an important aspect of current nanotechnology. In this study, Streptomyces aizuneusis ATCC 14921 gave the small particle of silver nanoparticles (AgNPs) a size of 38.45 nm, with 1.342 optical density. AgNPs produced by Streptomyces aizuneusis were characterized by means of UV-VIS spectroscopy and transmission electron microscopy (TEM). The UV-Vis spectrum of the aqueous solution containing silver ion showed a peak between 410 to 430. Moreover, the majority of nanoparticles were found to be a spherical shape with variables between 11 to 42 nm, as seen under TEM. The purity of extracted AgNPs was investigated by energy dispersive X-ray analysis (EDXA), and the identification of the possible biomolecules responsible for the reduction of Ag+ ions by the cell filtrate was carried out by Fourier Transform Infrared spectrum (FTIR). High antimicrobial activities were observed by AgNPs at a low concentration of 0.01 ppm, however, no deleterious effect of AgNPs was observed on the development and occurrence of Drosophila melanogaster phenotype. The highest reduction in the viability of the human lung carcinoma and normal cells was attained at 0.2 AgNPs ppm.

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