Biosynthesis of Zinc Oxide Nanoparticles Using Leaf Extract of Passiflora subpeltata: Characterization and Antibacterial Activity Against Escherichia coli Isolated from Poultry Faeces

Author(s):  
Udayashankar Arakere Chunchegowda ◽  
Ashwini Bagepalli Shivaram ◽  
Murali Mahadevamurthy ◽  
Lakshmeesha Thimappa Ramachndrappa ◽  
Sreelatha Gopalakrishna Lalitha ◽  
...  
2020 ◽  
Vol 2020 ◽  
pp. 1-9 ◽  
Author(s):  
Meron Girma Demissie ◽  
Fedlu Kedir Sabir ◽  
Gemechu Deressa Edossa ◽  
Bedasa Abdisa Gonfa

The synthesis of metal oxide nanoparticles with the use of medicinal plant extract is a promising alternative to the conventional chemical method. This work aimed to synthesize zinc oxide nanoparticles using a green approach from indigenous “Koseret” Lippia adoensis leaf extract which is an endemic medicinal plant and cultivated in home gardens of different regions of Ethiopia. The biosynthesized zinc oxide nanoparticles were characterized using thermogravimetric analysis, X-ray diffraction, scanning electron microscopy-energy dispersive spectroscopy, transmission electron microscopy, ultraviolet-visible spectroscopy, and Fourier transform infrared spectroscopy. Furthermore, this study also evaluated the antibacterial activity of the synthesized ZnO nanoparticles against clinical and standard strains of Escherichia coli, Klebsiella pneumonia, Staphylococcus aureus, and Enterococcus faecalis by the disc diffusion method. According to the result of this study, ZnO nanoparticles synthesized using Lippia adoensis leaf extract showed promising result against both Gram-positive and Gram-negative bacterial strains with a maximum inhibition zone of 14 mm and 12 mm, respectively, using uncalcinated form of the synthesized ZnO nanoparticles.


2017 ◽  
Vol 11 (1) ◽  
pp. 34-40
Author(s):  
Israa Ali Zaidan Al-Ogaidi

In the current study, synthesis and characterization of Zinc oxide nanoparticles (ZnONPs) and its application as anti-pathogenic bacteria were investigated.  ZnO which has been prepared by using aqueous of green tea leaves extract (Camellia sinensis) as a reducing agent. The wavelength range was measured by Ultraviolet–visible spectroscopy (UV-Vis) for monitoring the formation of the nanoparticles, which showed sharp peak at 360 nm. The average size and shape of the nanoparticles were detected by using Atomic Force Microscopy (AFM) which was 88 nm with spherical shape. Fourier transform–infrared (FTIR). FT-IR spectra was documented for the ZnO nanoparticles synthesized by green tea extract to detect the biomolecules involved in the synthesis process. The antibacterial activity of crystal Zinc Oxide (ZnO) nanoparticles was explored against pathogenic bacteria that included Escherichia coli, Staphylococcus aureus, and Acinetobacter baumannii. The antibacterial test was conducted in solid media using different concentrations of ZnO and disk diffusion method, 100 µg/ml presented the best antibacterial activity, and further studies on the damage of bacterial genomic DNA of Escherichia coli and Acinetobacter baumannii were carried out using gel electrophoresis exposed the DNA fragment bands, this activity may be caused by the interactions between the surface charge of cell and nanoparticles. Reactive oxygen species (ROS) properties of the particles might disturb cell wall and great antimicrobial action


2018 ◽  
Vol 8 (3) ◽  
pp. 293-299 ◽  
Author(s):  
G. Sharmila ◽  
C. Muthukumaran ◽  
K. Sandiya ◽  
S. Santhiya ◽  
R. Sakthi Pradeep ◽  
...  

2019 ◽  
Vol 17 ◽  
pp. 208-211 ◽  
Author(s):  
Agarwal Happy ◽  
Menon Soumya ◽  
S. Venkat Kumar ◽  
S. Rajeshkumar ◽  
R. David Sheba ◽  
...  

2020 ◽  
Vol 32 (10) ◽  
pp. 2589-2593
Author(s):  
Juhi Aggarwal ◽  
Tanveer Alam

Present paper deals with the synthesis of zinc oxide nanoparticles (ZnONPs) using leaf extract of Aristolochia elegans and study of antibacterial property for some human bacterial pathogens. The ZnONPs synthesized were characterized using UV-Vis, FT-IR, XRD, EDX, TEM and SEM techniques. The synthesized ZnONP having a crystallite size of 20.1 nm exhibited a distinct absorption peak maxima at 358 nm. The ZnONPs synthesized using the extract of A. elegans have shown antibacterial activity against M. luteus, S. aureus (Gram-positive), E. coli and P. aeruginosa (Gram-negative).


Author(s):  
Hams H. H. Alfattli ◽  
Ghufran Zuhair Jiber ◽  
Ghaidaa Gatea Abbass

This study which designed to evaluate the inhibitory effect of Ethanolic extract of (Quercusrobur) and Zinc oxide nanoparticles on the growth of one genus of enterobacteriacae (Salmonella). In vitro. For this purpose graduate concentrates for plant extract (50, 100, 200, 400 )mg/ml which prepared and compared with Zinc oxide nanoparticles of different concentration (2, 1, 0.5, 0.25) μg/ml,and examined. The result showed that the studied medicinal plant has antibacterial activity against this bacteria which used. The result showed that the plant has good activity in decrease the growth of this bacteria. The results of the study also showed that the nano-ZnO has very effective antibacterial action against the studied bacteria which was Salmonella,nanoparticles concentrations lead to increasing in the inhibition zones of tested bacterial growth. We also study the effect of three antibiotics Lomefloxacin (LOM), Ciprofloxacin (SIP) and Rifampin (RA) and the result showed,in a comparison within the tested bacteria,Salmonella had a significant inhibition increase in Lomefloxacin ; the ciprofloxacin showed effect on tested bacteria. However,Rifampin does not show any effect on tested bacteria.


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