Study on the STAT5A/AvaI polymorphism in Jersey cows and association with milk production traits

The aim of this study was to investigate associations between PPARGC1A genotypes and haplotypes and milk production traits. The study included 181 Jersey cows. The genotypes were identified by the PCR-RFLP method. The frequencies of the most common alleles were as follows: T – 0.63 (c.1892T>C) and A – 0.88 (c.3359A>C). The frequency of the most common haplotype was TC/AA – 0.558. The results showed that there were no statistically significant associations between the individual genotypes of both SNPs and milk traits; however, individuals with the CC/AC haplotype produced significantly (P ≤ 0.05; P ≤ 0.001) less milk and had a significantly (P ≤ 0.001) higher protein content in milk.

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Association of genetic variants of bovine <i>prolactin</i> with milk production traits of Black-and-White and Jersey cattle

Archives Animal Breeding ◽

10.5194/aab-48-149-2005 ◽

2005 ◽

Vol 48 (2) ◽

pp. 149-156 ◽

Cited By ~ 5

Author(s):

A. Dybus ◽

W. Grzesiak ◽

H. Kamieniecki ◽

I. Szatkowska ◽

Z. Sobek ◽

...

Keyword(s):

Milk Production ◽

Production Traits ◽

Milk Production Traits ◽

Black And White ◽

Jersey Cattle ◽

Prolactin Gene ◽

Pcr Rflp ◽

Rsai Polymorphism ◽

White Cattle ◽

Jersey Cows

Abstract. Associations between polymorphism localised in the third exon of the prolactin gene (PRL-RsaI) and milk xproduction traits of Black-and-White and Jersey cattle were analysed. A total of 427 cows were included in the study. PCR-RFLP method was used. The frequencies of genotypes and alleles were as follows: 0.7107 – AA, 0.2851 – AB, 0.0042 – BB; 0.8533 – PRLA and 0.1467 – PRLB for Black-and-White cattle and 0.0919 – AA, 0.4324 – AB, 0.4757 – BB; 0.3081 – PRLA and 0.6919 – PRLB for Jersey cattle. Statistically significant differences between the breeds were observed in the frequencies of genotypes and alleles. Associations between PRL-RsaI polymorphism and milk production traits of Jersey cows and lack of associations with these traits in Black-and-White cows were observed.

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Amplification created restriction sites for genotyping SNPs in the bovine ABCG2 and its association with milk production traits (Brief Report)

Archives Animal Breeding ◽

10.5194/aab-52-647-2009 ◽

2009 ◽

Vol 52 (6) ◽

pp. 647-649

Author(s):

I. Kowalewska-Łuczak ◽

H. Kulig ◽

M. Kmieć

Keyword(s):

Milk Production ◽

Mammary Epithelial Cells ◽

Mammary Epithelial ◽

Production Traits ◽

Milk Production Traits ◽

Pcr Rflp ◽

Restriction Sites ◽

Mouse Milk ◽

Jersey Cows ◽

Abcg2 Gene

Abstract. ABCG2 (ATP-binding cassette, subfamily G, member 2) belongs to the superfamily of ATPbinding cassette (ABC) transporters. In ATP-dependent processes, ABCG2 is responsible for transporting xenobiotics and cytostatic drugs across various cellular membranes. The ABCG2 gene is expressed in the apical membrane of alveolar mammary epithelial cells and is responsible for the active secretion of substrates into mouse milk. Other members of the ABC subfamily G are sterol transporters. It therefore appears that ABCG2 might transport cholesterol into milk (COHEN-ZINDER et al. 2005). In the study by COHEN-ZINDER et al. (2005), several SNPs were detected in the ABCG2 gene but only two were genotyped – in exon 14 and in intron 3. In the case of SNP A/C in exon 14 resulting in an amino acid change Y581S, it was demonstrated that this substitution affects milk yield and composition. To detect theses polymorphisms in our study we used a new PCR-RFLP method based on an amplification created restriction site (ACRS). This method has been frequently used by various researchers in recent times (e.g. ZYCH et al. 2007). The aim of this study was to estimate the frequencies of genotypes and alleles and to investigate possible associations between ABCG2 polymorphisms and milk production traits in Jersey cows.

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Effect of <i>SCD</i> SNPs on milk production traits of Jersey cows (Brief Report)

Archives Animal Breeding ◽

10.5194/aab-53-116-2010 ◽

2010 ◽

Vol 53 (1) ◽

pp. 116-118 ◽

Cited By ~ 1

Author(s):

H. Kulig ◽

I. Kowalewska-Łuczak ◽

M. Kmieć

Keyword(s):

Fatty Acids ◽

Milk Production ◽

Large Scale ◽

Saturated Fatty Acids ◽

Monounsaturated Fatty Acids ◽

Production Traits ◽

Milk Production Traits ◽

Gene Encoding ◽

Stearoyl Coa Desaturase ◽

Jersey Cows

Abstract. Studies concerning associations between candidate gene polymorphisms and milk production traits in cattle are carried out on a large scale (CITEK et al. 2007, KULIG 2005). Stearoyl-CoA desaturase (SCD) is the enzyme involved in conversion of saturated fatty acids into monounsaturated fatty acids. The gene encoding SCD was mapped to bovine chromosome 26 (CAMPBELL et al. 2001), where some QTLs for fat yield and other milk production traits as well as for somatic cells count (SCC) were also identified. Some of the SNPs identified within the SCD were significantly associated with fatty acid composition in milk and in carcass fat tissue (MOIOLI et al. 2007, TANIGUCHI et al. 2004). The effect of genotypes on milk production traits and SCC in cattle has not been reported so far. The aim of this study was therefore to establish possible associations between two SNPs in the SCD and milk production traits and SCC in Jersey cows.

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Two single nucleotide polymorphisms within bovine butyrophilin gene (BTN/HaeIII and BTN/SchI) and their association with milk performance traits in Jersey cattle

Archives Animal Breeding ◽

10.5194/aab-53-501-2010 ◽

2010 ◽

Vol 53 (5) ◽

pp. 501-509

Author(s):

M. Muszyńska ◽

I. Szatkowska ◽

W. Grzesiak ◽

A. Dybus ◽

D. Zaborski

Keyword(s):

Milk Production ◽

Fat Content ◽

Lower Percentage ◽

Nucleotide Polymorphisms ◽

Production Traits ◽

Milk Production Traits ◽

Jersey Cattle ◽

Pcr Rflp ◽

Milk Performance ◽

Jersey Cows

Abstract. Associations between two polymorphisms localized in the seventh exon of the butyrophilin gene (BTN/HaeIII and BTN/SchI) and milk production traits of Jersey cattle were analysed. A total of 171 cows were included in the study. PCR-RFLP method was used. In both polymorphisms three genotypes were identified. Statistically significant differences between polymorphic variants and milk production traits of Jersey cows were observed. Those differences concerned the percentage fat content in milk in the case of both polymorphisms and additionally the yield of milk and protein in the case of BTN/HaeIII polymorphism. The influence of the combined genotypes (BTN/HaeIII/SchI) on the examined traits was also analysed. GGHaeIII/AGSchI combined genotype was characterized by a significantly lower percentage fat content in comparison to other combinations. Moreover, incomplete linkage between the studied polymorphic sites was observed, despite relatively small distance between them.

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Effect of Breed and Some Non - Genetic Factors on Milk Production and Some Proportions of Its Chemical Components in Two Breeds of Local Sheep

Al-Qadisiyah Journal For Agriculture Sciences (QJAS) (P-ISSN 2077-5822 E-ISSN 2617-1479) ◽

10.33794/qjas.vol10.iss1.102 ◽

2020 ◽

Vol 10 (1) ◽

pp. 227-231

Author(s):

Rahman Hussein AL-Qasimi ◽

Shatha Mohammed Abbas ◽

Allawi L.D. AL-Khauzai

Keyword(s):

Milk Production ◽

Genetic Factors ◽

Chemical Components ◽

Lactation Period ◽

Production Traits ◽

Milk Production Traits ◽

Fat Percentage ◽

Awassi Sheep ◽

Total Milk ◽

Milk Components

The study was carried out on 19 ewes of local Awassi sheep and 12ewes local Arabi sheep in the Al-kafeel sheep station Karbala, to determine the effect of breed and some non-genetic factors such as (sex of the lamb, type of birth, age and weight of ewes at birth) on daily and total milk production and lactation period and some of milk components (fat, protein and lactose). The results showed that a significant effect (P <0.05) of the breed on milk production traits where Awassi sheep recorded the highest mean (0.91 kg , 101.63 kg , 104.86 day) compared to the Arabi sheep she was means (0.77 kg , 88.15 kg , 99.15 day) respectively. As well as in proportions of milk components with mean( 5.1 , 4.90 , 5.51) % respectively compared to the Arabi sheep (4.70 . 4.20 . 4.89) ewes with male lambs also exceeded superior ewes with female lambs in daily and total milk production and the lactation period the sex of the lamb did not affect the proportions of milk components the weight of the ewes had a significant effect (P <0.05) in milk production attributes with superior weight of ewes on lower ewes and did not affect the proportions of milk ingredients except for lactose. The type of birth and the age of the ewes did not have a significant effect in all the studied traits except for the superiority (P<0.05) of young ewes on age ewes in the fat percentage of milk.

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An association analysis between PRL genotype and milk production traits in Italian Mediterranean river buffalo

Journal of Dairy Research ◽

10.1017/s0022029917000693 ◽

2017 ◽

Vol 84 (4) ◽

pp. 430-433 ◽

Cited By ~ 6

Author(s):

Jun Li ◽

Aixin Liang ◽

Zipeng Li ◽

Chao Du ◽

Guohua Hua ◽

...

Keyword(s):

Milk Production ◽

Milk Yield ◽

Milk Fat ◽

Fat Content ◽

Phenotypic Variance ◽

River Buffalo ◽

Production Traits ◽

Milk Production Traits ◽

Exon 2 ◽

Mediterranean River

This Research Communication describes the association between genetic variation within the prolactin (PRL) gene and the milk production traits of Italian Mediterranean river buffalo (Bufala mediterranea Italiana). High resolution melting (HRM) techniques were developed for genotyping 465 buffaloes. The association of genetic polymorphism with milk production traits was performed and subsequently the effects of parity and calving season were evaluated. Single nucleotide polymorphisms (SNPs) were identified at exons 2 and 5 and at introns 1 and 2. All the SNPs were in Hardy–Weinberg equilibrium, and statistical analysis showed that the polymorphism of intron1 was significantly (P < 0·05) associated with milk yield, milk protein content and peak milk yield. The average contribution of the intron1 genotype (r2intron1) to total phenotypic variance in milk production traits was 0·09, and the TT genotype showed lower values than CC and CT genotypes. A nonsynonymous SNP was identified in exon 2, which resulted in an amino acid change from arginine to cysteine. Moreover, the polymorphism of exon 2 was associated significantly with milk fat content (P < 0·05), and the buffaloes with TT genotype showed higher total fat content than the buffaloes with CT genotype. These findings provide evidence that polymorphisms of the buffalo PRL gene are associated with milk production traits and PRL can be used as a candidate gene for marker-assisted selection in Italian Mediterranean river buffalo breeding.

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Factors influencing milk osteopontin concentration based on measurements from Danish Holstein cows

Journal of Dairy Research ◽

10.1017/s0022029921000054 ◽

2021 ◽

pp. 1-6

Author(s):

Brian Christensen ◽

Elias D. Zachariae ◽

Nina A. Poulsen ◽

Albert J. Buitenhuis ◽

Lotte B. Larsen ◽

...

Keyword(s):

Milk Production ◽

Bovine Milk ◽

Farm Management ◽

Holstein Cows ◽

Enzyme Linked Immunosorbent Assay ◽

Production Traits ◽

Milk Production Traits ◽

Factors Influencing ◽

Significant Interest ◽

Variation Explained

Abstract Our objective was to determine the content of the bioactive protein osteopontin (OPN) in bovine milk and identify factors influencing its concentration. OPN is expressed in many tissues and body fluids, with by far the highest concentrations in milk. OPN plays a role in immunological and developmental processes and it has been associated with several milk production traits and lactation persistency in cows. In the present study, we report the development of an enzyme linked immunosorbent assay (ELISA) for measurement of OPN in bovine milk. The method was used to determine the concentration of OPN in milk from 661 individual Danish Holstein cows. The median OPN level was determined to 21.9 mg/l with a pronounced level of individual variation ranging from 0.4 mg/l to 67.8 mg/l. Breeding for increased OPN in cow's milk is of significant interest, however, the heritability of OPN in milk was found to be relatively low, with an estimated value of 0.19 in the current dataset. The variation explained by the herd was also found to be low suggesting that OPN levels are not affected by farm management or feeding. Interestingly, the concentration of OPN was found to increase with days in milk and to decrease with parity.

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