Toxic effects of antimony on the seed germination and seedlings accumulation in Raphanus sativus L. radish and Brassica napus L.

2018 ◽  
Vol 45 (6) ◽  
pp. 2609-2614 ◽  
Author(s):  
Shu-xuan Liang ◽  
Ning Gao ◽  
Xiliang Li ◽  
Xiaocan Xi
Keyword(s):  
Brassica Napus ◽  
Seed Germination ◽  
Raphanus Sativus ◽  
Toxic Effects ◽  
Brassica Napus L ◽  
Raphanus Sativus L

scholarly journals Activation of rape (Brassica napus L.) embryo during seed germination. V. The first zones of ultrastructural changes and their expansion

2014 ◽  
Vol 56 (1) ◽  
pp. 77-91 ◽  
Author(s):  
Mieczysław Karaś
Keyword(s):  
Brassica Napus ◽  
Seed Germination ◽  
Seed Coat ◽  
Basal Part ◽  
Ultrastructural Changes ◽  
Embryo Axis ◽  
Brassica Napus L ◽  
Root Cells ◽  
Basal Zone

In the germinating rape embryo the columella and basal part of hypocotyl undergo earliest activation. Its first ultrastructural symptom is the appearance of numerous ER vesicles after 3-6 h of seed swelling. Their number is the highest in the external layers of the columella and decreases in basipetal direction. Dermatogen cells in the basal zone of the hypocotyl contain the greatest amount of ER structures, whereas decreasing amounts are found in both directions along the embryo axis and centripetally. Further changes in the ER spread in a similar order. The vesicles merge and form a tubular and plate-like ER. Then, they disappear and are replaced by tubular and vesicular forms. The changes in the ER are gradually followed by ultrastructural symptoms of activation of mitochondria, plastids and dictyosomes. The highest number of ER structures and other organelles accumulate in root cells shortly before piercing of the seed coat. After germination their amount decreases and remains almost stable.

scholarly journals Cloning, Sequence Analysis and Expression Patterns during Seed Germination of a Rapeseed (Brassica napus L.) G-x-S-x-G-motif Lipase Gene

2016 ◽  
Vol 44 (2) ◽  
pp. 435-444
Author(s):  
Imen GLAIED GHRAM ◽  
Hatem BELGUITH ◽  
Maha BEN MUSTAPHA ◽  
Issam HIMILA ◽  
Balkiss BOUHAOUALA ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Seed Germination ◽  
Expression Patterns ◽  
Pcr Amplification ◽  
Gene Families ◽  
Lipase Gene ◽  
Homologous Proteins ◽  
Brassica Napus L ◽  
Calculated Molecular Mass ◽  
Hydrolysis Of

Lipases catalyze the hydrolysis of ester bonds in triacylglycerides, generating glycerol and free fatty acids. These enzymes are encoded by extremely complex gene families, and appear to fulfil many different biological functions. Although they are present in all types of organisms, available information on plant lipases is still very limited, as compared to their bacterial and animal counterparts. A full-length clone, BnLIP, encoding a putative lipase, has been isolated by PCR amplification of Brassica napus genomic DNA, with oligonucleotide primers derived from the sequence of an Arabidopsis thaliana homologue. The clone included an open reading frame of 1581 bp encoding a polypeptide of 526 amino acids, with a calculated molecular mass of 59.5 kDa. Analysis of the deduced protein sequence, sequence alignment with homologous proteins from related plant species, and a phylogenetic analysis revealed that the BnLIP protein belongs to the ‘classical’ GxSxG-motif lipase family. RT-PCR assays indicated that the BnLIP gene is expressed specifically, but only transiently, during seed germination: the lipase mRNA was not present at detectable levels in ungerminated seeds, was detected only three days after seed imbibition, but its levels decreased rapidly afterwards. No expression was observed in roots, stems or leaves of adult plants. This expression pattern suggests that BnLIP is one of the lipases involved in the hydrolysis of triacylglycerides stored in rapeseed seeds, ultimately providing nutrients and energy to sustain seedling growth until photosynthesis is activated.

scholarly journals Heat stress during seed filling interferes with sulfur restriction on grain composition and seed germination in oilseed rape (Brassica napus L.)

2015 ◽  
Vol 6 ◽  
Author(s):  
Sophie Brunel-Muguet ◽  
Philippe D'Hooghe ◽  
Marie-Paule Bataillé ◽  
Colette Larré ◽  
Tae-Hwan Kim ◽  
...  
Keyword(s):  
Heat Stress ◽  
Brassica Napus ◽  
Seed Germination ◽  
Oilseed Rape ◽  
Grain Composition ◽  
Brassica Napus L ◽  
Seed Filling

scholarly journals From Dimness to Glossiness—Characteristics of the Spring Rapeseed Mutant Form without Glaucous Bloom (Brassica napus L.)

Agronomy ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1563
Author(s):  
Anna V. Shirokova ◽  
Valentina T. Volovik ◽  
Natalia V. Zagoskina ◽  
Georgiy P. Zaitsev ◽  
Hatima K. Khudyakova ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Seed Germination ◽  
Shoot Growth ◽  
Lignin Content ◽  
Seedling Development ◽  
Mutant Form ◽  
Brassica Napus L ◽  
Spring Canola ◽  
Wax Coating ◽  
Dry Out

As a result of the treatment of “Vikros” spring canola with the chemical mutagen ethyl methanesulfonate (EMS), a high-protein mutant form without glaucous bloom (wax bloom) on leaves, shoots, and siliques was isolated. Segregation into glossy and glaucous forms was always observed in the progeny of glossy plants from self-pollination, and the proportion of glaucous plants could reach up to 25%. The progeny of glaucous plants were homogeneous and did not segregate. If during the period of seed germination and seedling development the soil did not dry out and remained moist, and the average daily temperature did not exceed 16 °C, then the amount of glossy plants could reach 99%. Glossy plants possessed qualities valuable for breeding forage varieties, such as the increased content of protein in seeds (more than 30%), and change phenol metabolism, чтo прoявляется a reduced amount of lignin and sinapine in comparison with the original cultivar. In addition, plants without wax coating showed weakened shoot growth, decreased pollen fertility and seed production, and reduced lignin content in the shoots. Glossy mutants are of interest for the obtaining of fodder low-sinapine and low-lignin varieties of spring rapeseed.

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