Effect of Chemical Binding of Doxorubicin Hydrochloride to Gold Nanoparticles, Versus Electrostatic Adsorption, on the In Vitro Drug Release and Cytotoxicity to Breast Cancer Cells

2018 ◽  
Vol 35 (6) ◽  
Author(s):  
Gamal M. Zayed ◽  
Islam Kamal ◽  
Wael A. Abdelhafez ◽  
Fahd M. Alsharif ◽  
Mohamed A. Amin ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Gold Nanoparticles ◽  
Drug Release ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Doxorubicin Hydrochloride ◽  
In Vitro Drug Release ◽  
Electrostatic Adsorption ◽  
Chemical Binding

scholarly journals Development and Characterization of Nanoliposomal Hydroxyurea Against BT-474 Breast Cancer Cells

2019 ◽  
Vol 10 (1) ◽  
pp. 39-45 ◽  
Author(s):  
Azam Akbari ◽  
Azim Akbarzadeh ◽  
Morteza Rafiee Tehrani ◽  
Reza Ahangari Cohan ◽  
Mohsen Chiani ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Drug Release ◽  
Zeta Potential ◽  
Cancer Cells ◽  
Cellular Uptake ◽  
Breast Cancer Cells ◽  
Drug Carriers ◽  
Diffusion Method ◽  
Diphenyl Tetrazolium Bromide

Purpose: Hydroxyurea (HU) is a well-known chemotherapy drug with several side effects which limit its clinical application. This study was conducted to improve its therapeutic efficiency against breast cancer using liposomes as FDA-approved drug carriers. Methods: PEGylated nanoliposomes-containing HU (NL-HU) were made via a thin-film hydration method, and assessed in terms of zeta potential, size, morphology, release, stability, cellular uptake, and cytotoxicity. The particle size and zeta potential of NL-HU were specified by zeta-sizer. The drug release from liposomes was assessed by dialysis diffusion method. Cellular uptake was evaluated by flow cytometry. The cytotoxicity was designated by methyl thiazolyl diphenyl-tetrazolium bromide (MTT) test. Results: The size and zeta value of NL-HU were gotten as 85 nm and -27 mV, respectively. NL-HU were spherical.NL-HU vesicles were detected to be stable for two months. The slow drug release and Weibull kinetic model were obtained. Liposomes considerably enhanced the uptake of HU into BT-474 human breast cancer cells. The cytotoxicity of NL-HU on BT-474 cells was found to be significantly more than that of free HU. Conclusion: The results confirmed these PEGylated nanoliposomes containing drug are potentially suitable against in vitro model of breast cancer.

Preparation and In-vitro Evaluation of Levan Micelles: A Polyfructan Based Nano-carrier for Breast Cancer Targeted Delivery

2019 ◽  
Vol 9 (2) ◽  
pp. 97-107 ◽  
Author(s):  
Parth Patel ◽  
Yadvendrakumar Agrawal
Keyword(s):  
Breast Cancer ◽  
Kinetic Model ◽  
Drug Release ◽  
Critical Micelle Concentration ◽  
Cancer Cells ◽  
Cell Line ◽  
Targeted Delivery ◽  
Breast Cancer Cells ◽  
Ic50 Value

Background: Levans are biopolymers of fructose, produced by different microorganisms. Fructose present in the levan micelles binds with the Glucose Transporter 5 (GLUT 5) which is overexpressed in the breast cancer cells. Objective: Increased solubility of paclitaxel by loading in the GLUT 5 transporter targeted levan-based micelles may enhance its bioavailability and facilitate a targeted delivery to the breast cancer cells. Methods and Results: Critical micelle concentration of levan with an average molecular weight of 800,000 Dalton was found to be 0.125µM corresponding to 0.1mg/mL using pyrene I3/I1 method. At critical micelle concentration (CMC), levan formed very mono-disperse (PDI-0.082) micellar particles with a particle size of 153.1 ± 2.31nm and -14.6 ± 2mV zeta potential. In-vitro drug release study was performed to identify the fit kinetic model along with Fourier transform infrared analysis and Differential scanning calorimetry studies. In-vitro kinetic model fitting revealed first-order drug release from the prepared micellar composition. The drug-loaded micellar composition was studied for its anticancer activity in breast cancer cell line. The IC50 value obtained was 1.525 ± 0.11nM on MCF7 cell line. Conclusion: Paclitaxel micelles showed a nineteen-fold improvement in the IC50 value compared to free paclitaxel. Hemocompatibility study was performed with a view to parenteral administration. This solution containing drug was found to be hemocompatible when added to bovine blood in 1:4 ration. Micelles are proven fairly compatible on the basis of hemolysis test results.

scholarly journals Histidine-Tagged Folate-Targeted Gold Nanoparticles for Enhanced Transgene Expression in Breast Cancer Cells In Vitro

Pharmaceutics ◽  
2021 ◽  
Vol 14 (1) ◽  
pp. 53
Author(s):  
Calrin Joseph ◽  
Aliscia Daniels ◽  
Sooboo Singh ◽  
Moganavelli Singh
Keyword(s):  
Breast Cancer ◽  
Gold Nanoparticles ◽  
Gene Delivery ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Folate Receptor ◽  
Endosomal Escape ◽  
Delivery Vehicles ◽  
Gene Delivery Vehicles

Nanotechnology has emerged as a promising treatment strategy in gene therapy, especially against diseases such as cancer. Gold nanoparticles (AuNPs) are regarded as favorable gene delivery vehicles due to their low toxicity, ease of synthesis and ability to be functionalized. This study aimed to prepare functionalized AuNPs (FAuNPs) and evaluate their folate-targeted and nontargeted pCMV-Luc-DNA delivery in breast cancer cells in vitro. CS was added to induce stability and positive charges to the AuNPs (Au-CS), histidine (Au-CS-His) to enhance endosomal escape and folic acid for folate-receptor targeting (Au-CS-FA-His). The FAuNP:pDNA nanocomplexes possessed favorable sizes (<135 nm) and zeta potentials (<−20 mV), strong compaction efficiency and were capable of pDNA protection against nuclease degradation. These nanocomplexes showed minimal cytotoxicity (>73% cell viability) and enhanced transgene activity. The influence of His was notable in the HER2 overexpressing SKBR3 cells, which produced higher gene expression. Furthermore, the FA-targeted nanocomplexes enhanced receptor-mediated endocytosis, especially in MCF-7 cells, as confirmed by the receptor competition assay. While the role of His may need further optimization, the results achieved suggest that these FAuNPs may be suitable gene delivery vehicles for breast cancer therapeutics.

scholarly journals Spectral Photon-Counting Molecular Imaging for Quantification of Monoclonal Antibody-Conjugated Gold Nanoparticles Targeted to Lymphoma and Breast Cancer: An In Vitro Study

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Mahdieh Moghiseh ◽  
Chiara Lowe ◽  
John G. Lewis ◽  
Dhiraj Kumar ◽  
Anthony Butler ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Monoclonal Antibody ◽  
Gold Nanoparticles ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Photon Counting ◽  
Spectral Ct ◽  
Her2 Positive ◽  
Raji Cells

The purpose of the present study was to demonstrate an in vitro proof of principle that spectral photon-counting CT can measure gold-labelled specific antibodies targeted to specific cancer cells. A crossover study was performed with Raji lymphoma cancer cells and HER2-positive SKBR3 breast cancer cells using a MARS spectral CT scanner. Raji cells were incubated with monoclonal antibody-labelled gold, rituximab (specific antibody to Raji cells), and trastuzumab (as a control); HER2-positive SKBR3 breast cancer cells were incubated with monoclonal antibody-labelled gold, trastuzumab (specific antibody to HER2-positive cancer cells), and rituximab (as a control). The calibration vials with multiple concentrations of nonfunctionalised gold nanoparticles were used to calibrate spectral CT. Spectral imaging results showed that the Raji cells-rituximab-gold and HER2-positive cells-trastuzumab-gold had a quantifiable amount of gold, 5.97 mg and 0.78 mg, respectively. In contrast, both cell lines incubated with control antibody-labelled gold nanoparticles had less gold attached (1.22 mg and 0.15 mg, respectively). These results demonstrate the proof of principle that spectral molecular CT imaging can identify and quantify specific monoclonal antibody-labelled gold nanoparticles taken up by Raji cells and HER2-positive SKBR3 breast cancer cells. The present study reports the future potential of spectral molecular imaging in detecting tumour heterogeneity so that treatment can be tuned accordingly, leading to more effective personalised medicine.

scholarly journals ID:2024 Synthesis and Characterization of PLGA-PEG Thymoquinone Nanoparticle and its Cytotoxicity Effects in Tamoxifen-resistant Breast Cancer Cells

2017 ◽  
Vol 4 (S) ◽  
pp. 55 ◽  
Author(s):  
Rozaina Ahmad ◽  
Noor Haida Mohd Kaus ◽  
Shahrul Hamid
Keyword(s):  
Breast Cancer ◽  
Drug Release ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Spherical Shape ◽  
Entrapment Efficiency ◽  
Volatile Oil ◽  
Pluronic F68

Background: Drug resistance has been a continuous challenge in cancer treatment. The use of nanotechnology in the development of new cancer drugs has potential. One of the extensively studied compound is thymoquinone (TQ) and this work aims to compare two types of TQ-nanoformulation and its cytotoxicity towards resistant cancer cells. Methods: TQ-nanoparticles were prepared and optimized by using 2 different formulation with different drug to PLGA-PEG ratio (1:20 and 1:7) and different PLGA-PEG to pluronic F68 ratio (10:1 and 2:1). The morphology and size were determined using TEM and DLS. Characterization of particles were done using UV-VIS, ATR-IR, entrapment efficiency and drug release. The effects of drug, polymer and surfactants were compared between the two formulations. Cytotoxicity assay was performed using MTS assay. Results: TEM finding showed 96% of particles produced with 1:7 drug to PLGA-PEG were less than 90 nm in size and spherical in shape. This was confirmed with DLS which showed smaller particle size than those formed with 1:20 drug to PLGA-PEG ration. Further analysis showed zeta potential was negatively charge which could facilitate cellular uptake as reported previously. In addition, PDI value was less than 0.1 in both formulations indicating monodispersed and less broad in size distribution. The absorption peak of PLGA-PEG-TQ-Nps were at 255 nm. The 1:7 drug to polymer formulation was selected for further analysis where the entrapment efficiency was 79.9% and in vitro drug release showed a maximum release of TQ at 64% respectively. Cytotoxicity result showed IC50 of TQ-nanoparticle at 20.05 µM and free TQ was 8.25 µM. Conclusion: This study showed that nanoparticle synthesized with 1:7 drug to PLGA-PEG ratio and 2:1 PLGA-PEG to pluronic F68 were less than 100 nm and had spherical shape as confirmed with DLS. This could facilitate its transportation and absorption to reach its target. There was conserved TQ stability as exhibited by slow release of this volatile oil. Cytotoxicity effect was noted when resistant breast cancer cells were treated with these particles.

scholarly journals Effect of Modification Protocols on the Effectiveness of Gold Nanoparticles as Drug Delivery Vehicles for Killing of Breast Cancer Cells

2016 ◽  
Vol 69 (12) ◽  
pp. 1402 ◽  
Author(s):  
Zahrah Alhalili ◽  
Daniela Figueroa ◽  
Martin R. Johnston ◽  
Joe Shapter ◽  
Barbara Sanderson
Keyword(s):  
Breast Cancer ◽  
Cell Culture ◽  
Gold Nanoparticles ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Culture Media ◽  
High Dose ◽  
Dependent Manner ◽  
T47d Cells

The current study evaluated the potential of gold nanoparticles (AuNPs) for the delivery of Taxol to breast cancer cells (T47D) using an in vitro cell culture model. For this study, new loading approaches and novel chemical attachments were investigated. Five different gold nanoparticle-based complexes were used to determine their cytotoxicity towards T47D cells using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) viability assay. There was no significant decrease (P > 0.05) in cell viability when T47D cells were treated with AuNPs that did not contain Taxol. However, cells were significantly killed by gold nanoparticles chemically conjugated to Taxol using three different approaches and one novel hybrid AuNP-Taxol nanoparticle, wherein no chemical bonds were involved. These Taxol-loaded AuNPs were more effective at inducing cell death in vitro than a solution of free Taxol used to treat cells. This result demonstrated that Taxol could be released from the particles in the cell culture media for subsequent therapeutic action. Additionally, the experiments proved that the Taxol-loaded AuNPs were more toxic in a dose dependent manner than Taxol as a formulation for the treatment of breast cancer cells. The results of this study suggest that gold nanoparticles have potential for the efficient delivery of Taxol to breast cancer cells. This could provide a future solution as an alternative application method to overcome adverse side effects resulting from current high-dose treatment regimes.

Chitosan-gold nanoparticles trigger apoptosis in human breast cancer cells in vitro

The Nucleus ◽  
2020 ◽  
Author(s):  
Arindam Bandyopadhyay ◽  
Bishnupada Roy ◽  
Pallab Shaw ◽  
Paritosh Mondal ◽  
Maloy Kr. Mondal ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Gold Nanoparticles ◽  
Cancer Cells ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Human Breast Cancer Cells ◽  
Human Breast

scholarly journals A Therapeutic System of 177Lu-labeled Gold Nanoparticles-RGD Internalized in Breast Cancer Cells

2017 ◽  
Vol 57 (3) ◽  
Author(s):  
Myrna Luna-Gutiérrez ◽  
Guillermina Ferro-Flores ◽  
Blanca E. Ocampo-García ◽  
Clara L. Santos- Cuevas ◽  
Nallely Jiménez-Mancilla ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Gold Nanoparticles ◽  
Cancer Cells ◽  
Photothermal Therapy ◽  
Breast Cancer Cells ◽  
Mcf7 Cell ◽  
Thiol Group ◽  
20 Nm ◽  
Spontaneous Reaction

The aim of this research was to evaluate the <em>in vitro</em> potential of <sup>177</sup>Lu-labeled gold nanoparticles conjugated to cyclo-[RGDfK(C)] peptides (<sup>177</sup>Lu-AuNP-c[RGDfK(C)]) as a plasmonic photothermal therapy and targeted radiotherapy system in MCF7 breast cancer cells. Peptides were conjugated to AuNPs (20 nm) by spontaneous reaction with the thiol group of cysteine (C). After laser irradiation, the presence of c[RGDfK(C)]-AuNP in cells caused a significant increase in the temperature of the medium (50.5 °C, compared to 40.3 °C without AuNPs) resulting in a significant decrease in MCF7 cell viability down to 9 %. After treatment with <sup>177</sup>-AuNP-c[RGDfK(C)], the MCF7 cell proliferation was significantly inhibited.

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