Effect of Glomus mosseae on chlorophyll content, chlorophyll fluorescence parameters, and chloroplast ultrastructure of beach plum (Prunus maritima) under NaCl stress

Vita 3 and Vita 5 are two Vigna unguiculata cultivars that differ in their capacities for survival in saline environments; Vita 3 is more tolerant and Vita 5 more sensitive. Both cultivars were submitted to salt stress with 0.1 M NaCl. After 8 days, root and shoot growth from both cultivars was reduced but reduction was more pronounced in Vita 5. Furthermore, leaf area was also reduced in this cultivar. Chlorophyll content and chlorophyll fluorescence parameters were not affected by salt stress, but the specific activities of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) decreased in Vita 3 and increased in Vita 5. The use of immunological techniques also revealed that the Rubisco content from Vita 3 decreased while that of Vita 5 increased. The discussion of these results is aimed at reaching a better understanding of the differences between these cultivars in relation to salt stress.

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Physiological and Transcriptomic Analysis Reveals the Responses and Difference to High Temperature and Humidity Stress in Two Melon Genotypes

International Journal of Molecular Sciences ◽

10.3390/ijms23020734 ◽

2022 ◽

Vol 23 (2) ◽

pp. 734

Author(s):

Jinyang Weng ◽

Asad Rehman ◽

Pengli Li ◽

Liying Chang ◽

Yidong Zhang ◽

...

Keyword(s):

Chlorophyll Fluorescence ◽

High Temperature ◽

Photosystem Ii ◽

Photosystem I ◽

Chloroplast Ultrastructure ◽

Future Research ◽

Leaf Water Content ◽

Chlorophyll Fluorescence Parameters ◽

Fluorescence Parameters ◽

Temperature And Humidity

Due to the frequent occurrence of continuous high temperatures and heavy rain in summer, extremely high-temperature and high-humidity environments occur, which seriously harms crop growth. High temperature and humidity (HTH) stress have become the main environmental factors of combined stress in summer. The responses of morphological indexes, physiological and biochemical indexes, gas exchange parameters, and chlorophyll fluorescence parameters were measured and combined with chloroplast ultrastructure and transcriptome sequencing to analyze the reasons for the difference in tolerance to HTH stress in HTH-sensitive ‘JIN TAI LANG’ and HTH-tolerant ‘JIN DI’ varieties. The results showed that with the extension of stress time, the superoxide dismutase (SOD), peroxidase (POD), and ascorbate peroxidase (APX) activities of the two melon varieties increased rapidly, the leaf water content increased, and the tolerant varieties showed stronger antioxidant capacity. Among the sensitive cultivars, Pn, Fv/Fm, photosystem II, and photosystem I chlorophyll fluorescence parameters were severely inhibited and decreased rapidly with the extension of stress time, while the HTH-tolerant cultivars slightly decreased. The cell membrane and chloroplast damage in sensitive cultivars were more severe, and Lhca1, Lhca3, and Lhca4 proteins in photosystem II and Lhcb1-Lhcb6 proteins in photosystem I were inhibited compared with those in the tolerant cultivar. These conclusions may be the main reason for the different tolerances of the two cultivars. These findings will provide new insights into the response of other crops to HTH stress and also provide a basis for future research on the mechanism of HTH resistance in melon.

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Effects of NtSPS1 Overexpression on Solanesol Content, Plant Growth, Photosynthesis, and Metabolome of Nicotiana tabacum

Plants ◽

10.3390/plants9040518 ◽

2020 ◽

Vol 9 (4) ◽

pp. 518

Author(s):

Ning Yan ◽

Xiaolei Gai ◽

Lin Xue ◽

Yongmei Du ◽

John Shi ◽

...

Keyword(s):

Chlorophyll Fluorescence ◽

Nicotiana Tabacum ◽

Plant Growth ◽

Chlorophyll Content ◽

Carbon Fixation ◽

Leaf Growth ◽

Pathway Enrichment Analysis ◽

Photosynthetic Gas Exchange ◽

Chlorophyll Fluorescence Parameters ◽

Fluorescence Parameters

Nicotiana tabacum solanesyl diphosphate synthase 1 (NtSPS1) is the key enzyme in solanesol biosynthesis. However, changes in the solanesol content, plant growth, photosynthesis, and metabolome of tobacco plants after NtSPS1 overexpression (OE) have not been previously reported. In the present study, these parameters, as well as photosynthetic gas exchange, chlorophyll content, and chlorophyll fluorescence parameters, were compared between NtSPS1 OE and wild type (WT) lines of tobacco. As expected, NtSPS1 OE significantly increased solanesol content in tobacco leaves. Although NtSPS1 OE significantly increased leaf growth, photosynthesis, and chlorophyll content, the chlorophyll fluorescence parameters in the leaves of the NtSPS1 OE lines were only slightly higher than those in the WT leaves. Furthermore, NtSPS1 OE resulted in 64 differential metabolites, including 30 up-regulated and 34 down-regulated metabolites, between the OE and WT leaves. Pathway enrichment analysis of these differential metabolites identified differentially enriched pathways between the OE and WT leaves, e.g., carbon fixation in photosynthetic organisms. The maximum carboxylation rate of RuBisCO and the maximum rate of RuBP regeneration were also elevated in the NtSPS1 OE line. To our knowledge, this is the first study to confirm the role of NtSPS1 in solanesol biosynthesis and its possible functional mechanisms in tobacco.

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Effect of Branched Broomrape (Orobanche ramosa) Infection on the Growth and Photosynthesis of Tomato

Weed Science ◽

10.1614/ws-07-147.1 ◽

2008 ◽

Vol 56 (4) ◽

pp. 574-581 ◽

Cited By ~ 29

Author(s):

Giovanni Mauromicale ◽

Antonino Lo Monaco ◽

Angela M. G. Longo

Keyword(s):

Chlorophyll Fluorescence ◽

Chlorophyll Content ◽

Photosynthetic Rate ◽

Carbon Assimilation ◽

Parasitic Plants ◽

Chlorophyll Fluorescence Parameters ◽

Leaf Chlorophyll Content ◽

Fluorescence Parameters ◽

Growing Seasons ◽

Leaf Chlorophyll

The influence of the holoparasite branched broomrape on the vegetative growth, leaf chlorophyll content, photosynthetic rate, and chlorophyll fluorescence of tomato was studied over two growing seasons on plants grown in a commercial greenhouse. The presence of the parasite strongly reduced the aerial biomass by acting as a competing sink for assimilate, but more importantly, by compromising the efficiency of carbon assimilation via a reduction in leaf chlorophyll content and photosynthetic rate. The chlorophyll fluorescence parameters F0, Fm, Fv, and Fv/Fm were all altered in parasitized plants, indicating that branched broomrape–infected plants are more susceptible to photoinhibition. The degree of damage to the host was not dependent on either the number or the biomass of parasitic plants per host plant. We suggest that the ability to maintain a high photosynthetic rate, leaf chlorophyll content, or both and the ability to minimize photoinhibition can be developed as indirect assays for improved tolerance to branched broomrape.

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Effects of Drought Stress on Chlorophyll Fluorescence Parameters, Chlorophyll Content and Grain Yield of Wheat Cultivars

Journal of Biological Sciences ◽

10.3923/jbs.2007.841.847 ◽

2007 ◽

Vol 7 (6) ◽

pp. 841-847 ◽

Cited By ~ 44

Author(s):

Farzad Paknejad ◽

Mohammad Nasri . ◽

Hamid Reza Tohidi Mo . ◽

Hossein Zahedi . ◽

Majid Jami Alahmadi .

Keyword(s):

Chlorophyll Fluorescence ◽

Drought Stress ◽

Grain Yield ◽

Chlorophyll Content ◽

Wheat Cultivars ◽

Chlorophyll Fluorescence Parameters ◽

Fluorescence Parameters ◽

Effects Of Drought

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EFFECTS OF PESTICIDE ON THE CHLOROPHYLL FLUORESCENCE PARAMETERS IN NO-HEADING CHINESE CABBAGE

Acta Horticulturae ◽

10.17660/actahortic.2010.856.28 ◽

2010 ◽

pp. 211-216

Author(s):

Z.K. Gao ◽

T. Zhao ◽

M. Wang ◽

W.L. Zhang ◽

J.P. He ◽

...

Keyword(s):

Chlorophyll Fluorescence ◽

Chinese Cabbage ◽

Chlorophyll Fluorescence Parameters ◽

Fluorescence Parameters ◽

Heading Chinese Cabbage

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Photoinduced toxicity of fluoranthene on primary processes of photosynthesis in lichens

The Lichenologist ◽

10.1017/s0024282907006111 ◽

2006 ◽

Vol 39 (1) ◽

pp. 91-100 ◽

Cited By ~ 11

Author(s):

Marie KUMMEROVÁ ◽

štěpán ZEZULKA ◽

Jana KRULOVÁ ◽

Jan TŘÍSKA

Keyword(s):

Chlorophyll Fluorescence ◽

Bioconcentration Factor ◽

Lichen Species ◽

Chlorophyll Fluorescence Parameters ◽

Fluorescence Parameters ◽

Photochemical Processes ◽

Symbiotic Algae ◽

Lasallia Pustulata ◽

Photoinduced Toxicity ◽

Umbilicaria Hirsuta

The effect of increasing concentrations (0·01, 0·1, 1 and 5 mg l−1) of intact (FLT) and photo-modified (phFLT) fluoranthene and the duration of exposure (1, 2, 3, 5 and 7 days) on the chlorophyll fluorescence parameters (F0, FV/FM, and ΦII) of symbiotic algae in the thalli of two foliose lichens Lasallia pustulata and Umbilicaria hirsuta was investigated. In addition the FLT concentration in thalli of both lichen species was determined and a bioconcentration factor (BCF) was calculated. The results obtained demonstrated that the concentrations of FLT and especially phFLT (1 and 5 mg l−1) applied affected primary photochemical processes of photosynthesis in the algae of both lichen species. The F0 value increased and the FV/FM and ΦII values decreased. The fluoranthene content in the thallus of both lichen species increased with increasing FLT concentration in the environment.

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Photosynthetic response to nitrogen source and different ratios of nitrogen and phosphorus in toxic cyanobacteria, Microcystis aeruginosa FACHB-905

Journal of Limnology ◽

10.4081/jlimnol.2016.1458 ◽

2016 ◽

Cited By ~ 3

Author(s):

Guotao Peng ◽

Zhengqiu Fan ◽

Xiangrong Wang ◽

Chen Chen

Keyword(s):

Chlorophyll Fluorescence ◽

Algal Blooms ◽

Freshwater Ecosystems ◽

Cyanobacterial Blooms ◽

Photosynthetic Response ◽

Nitrogen And Phosphorus ◽

Ammonium Toxicity ◽

Chlorophyll Fluorescence Parameters ◽

Fluorescence Parameters ◽

N Assimilation

The frequent outbreak of cyanobacterial blooms has become a worldwide phenomenon in freshwater ecosystems. Studies have elucidated the close relationship between harmful algal blooms and nutrient contents, including the loading of nitrogen and the ratios of nitrogen (N) and phosphorus (P). In this study, the effect of inorganic (nitrate and ammonium) and organic (urea) nitrogen at varied N/P ratios on the Microcystis aeruginosa FACHB-905 accumulation and photosynthesis was investigated. The optimal NO3/P in this study were 30~50 indicated by the cell abundance (4.1×106/mL), pigment concentration (chlorophyll a 3.1 mg/L, phycocyanin 8.3mg/L), and chlorophyll fluorescence parameters (rETR, Ek, α, φPSII and Fv/Fm values), while too high NO3-N (N/P=100:1) would cause an intracellular nitrate inhibition, leading to a decrease of photosynthetic activity. In addition, low concentration of NH4-N (N/P=4:1) would favor the M. aeruginosa growth and photosynthesis, and high NH4/P ratio (>16) would rise the ammonium toxicity of algal cells and affect the N assimilation. In urea treatments, M. aeruginosa responded similarly to the NH4-N treatments both in growth curves and pigment contents, and the favorable N/P ratio was between 16~30, suggested by the chlorophyll fluorescence parameters. The results demonstrated that the various chemical forms of N and N/P ratios have a significant impact on Microcystis abundance and photosynthesis. More work is needed to figure out the mechanism of nitrogen utilization by Microcystis and the photosynthetic response to nutrient stress at the molecular level.

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