OsRMC, a negative regulator of salt stress response in rice, is regulated by two AP2/ERF transcription factors

Salt stress is considered to be the most severe abiotic stress. High soil salinity leads to osmotic and ionic toxicity, resulting in reduced plant growth and crop production. The role of G-proteins during salt stresses is well established. AGB1, a G-protein subunit, not only plays an important role during regulation of Na+ fluxes in roots, but is also involved in the translocation of Na+ from roots to shoots. N-Myc Downregulated like 1 (NDL1) is an interacting partner of G protein βγ subunits and C-4 domain of RGS1 in Arabidopsis. Our recent in-planta expression analysis of NDL1 reported changes in patterns during salt stress. Based on these expression profiles, we have carried out functional characterization of the AGB1-NDL1 module during salinity stress. Using various available mutant and overexpression lines of NDL1 and AGB1, we found that NDL1 acts as a negative regulator during salt stress response at the seedling stage, an opposite response to that of AGB1. On the other hand, during the germination phase of the plant, this role is reversed, indicating developmental and tissue specific regulation. To elucidate the mechanism of the AGB1-NDL1 module, we investigated the possible role of the three NDL1 stress specific interactors, namely ANNAT1, SLT1, and IDH-V, using yeast as a model. The present study revealed that NDL1 acts as a modulator of salt stress response, wherein it can have both positive as well as negative functions during salinity stress. Our findings suggest that the NDL1 mediated stress response depends on its developmental stage-specific expression patterns as well as the differential presence and interaction of the stress-specific interactors.

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PpSARK Regulates Moss Senescence and Salt Tolerance through ABA Related Pathway

International Journal of Molecular Sciences ◽

10.3390/ijms19092609 ◽

2018 ◽

Vol 19 (9) ◽

pp. 2609 ◽

Cited By ~ 2

Author(s):

Ping Li ◽

Hong Yang ◽

Gaojing Liu ◽

Wenzhang Ma ◽

Chuanhong Li ◽

...

Keyword(s):

Salt Stress ◽

Stress Response ◽

Salt Tolerance ◽

Stress Responses ◽

Physcomitrella Patens ◽

Stress Conditions ◽

Negative Regulator ◽

Salt Stress Response ◽

Functional Roles ◽

Related Pathway

Senescence-associated receptor-like kinase (SARK) family members in Arabidopsis, soybean, and rice are known to be positive regulators of leaf senescence. In the meantime, SARKs are extensively involved in stress response. However, their function and underlying molecular mechanism in stress responses in moss are not well known. Here, we investigated functional roles of SARK isolated from Physcomitrella patens (PpSARK) in salt stress response and senescence. PpSARK transcripts significantly accumulated under NaCl and abscisic acid (ABA) treatments, with higher expression in the moss gametophyte stage. Insertional gain-of-function mutants of PpSARK (PpSARKg) were more tolerant to salt stress and ABA than wild type (WT), whereas senescence of mutants was delayed during the protonema stage. Expression of stress-responsive genes in the ABA related pathway, such as PpABI3, PpABI5, PpPP2C, and PpLEA were significantly higher in PpSARKg and WT under salt stress conditions, suggesting that PpSARK might positively regulate salt tolerance via an ABA-related pathway. Endogenous ABA contents also increased 3-fold under salt stress conditions. These results indicate that PpSARK functions as a positive regulator in salt stress responses, while possibly functioning as a negative regulator in senescence in moss.

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BpTCP3 Transcription Factor Improves Salt Tolerance of Betula platyphylla by Reducing Reactive Oxygen Species Damage

Forests ◽

10.3390/f12121633 ◽

2021 ◽

Vol 12 (12) ◽

pp. 1633

Author(s):

Li Ren ◽

Fangrui Li ◽

Jing Jiang ◽

Huiyu Li

Keyword(s):

Reactive Oxygen Species ◽

Transcription Factor ◽

Transcription Factors ◽

Salt Stress ◽

Stress Response ◽

Salt Tolerance ◽

Reactive Oxygen ◽

Betula Platyphylla ◽

Oxygen Species ◽

Salt Stress Response

The plant-specific transcription factors TEOSINTE BRANCHED1/CYCLO IDEA/PROLIFERATING CELL FACTOR1 (TCP) act as developmental regulators that have many roles in the growth and development processes throughout the entire life span of plants. TCP transcription factors are responsive to endogenous and environmental signals, such as salt stress. However, studies on the role of the TCP genes in salt stress response have rarely focused on woody plants, especially forest trees. In this study, the BpTCP3 gene, a CYC/TB1 subfamily member, isolated from Betula platyphylla Sukaczev, was significantly influenced by salt stress. The β-glucuronidase (GUS) staining analysis of transgenic B. platyphylla harboring the BpTCP3 promoter fused to the reporter gene GUS (pBpTCP3::GUS) further confirmed that the BpTCP3 gene acts a positive regulatory position in salt stress. Under salt stress, we found that the BpTCP3 overexpressed lines had increased relative/absolute high growth but decreased salt damage index, hydrogen peroxide (H2O2), and malondialdehyde (MDA) levels versus wild-type (WT) plants. Conversely, the BpTCP3 suppressed lines exhibited sensitivity to salt stress. These results indicate that the BpTCP3 transcription factor improves the salt tolerance of B. platyphylla by reducing reactive oxygen species damage, which provides useful clues for the functions of the CYC/TB1 subfamily gene in the salt stress response of B. platyphylla.

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Involvement of ethylene receptors in the salt tolerance response of Cucurbita pepo

Horticulture Research ◽

10.1038/s41438-021-00508-z ◽

2021 ◽

Vol 8 (1) ◽

Author(s):

Gustavo Cebrián ◽

Jessica Iglesias-Moya ◽

Alicia García ◽

Javier Martínez ◽

Jonathan Romero ◽

...

Keyword(s):

Salt Stress ◽

Stress Response ◽

Salt Tolerance ◽

Vegetative Growth ◽

Cucurbita Pepo ◽

Compatible Solutes ◽

Negative Regulator ◽

Ethylene Receptors ◽

Salt Stress Response ◽

Tolerance Response

AbstractAbiotic stresses have a negative effect on crop production, affecting both vegetative and reproductive development. Ethylene plays a relevant role in plant response to environmental stresses, but the specific contribution of ethylene biosynthesis and signalling components in the salt stress response differs between Arabidopsis and rice, the two most studied model plants. In this paper, we study the effect of three gain-of-function mutations affecting the ethylene receptors CpETR1B, CpETR1A, and CpETR2B of Cucurbita pepo on salt stress response during germination, seedling establishment, and subsequent vegetative growth of plants. The mutations all reduced ethylene sensitivity, but enhanced salt tolerance, during both germination and vegetative growth, demonstrating that the three ethylene receptors play a positive role in salt tolerance. Under salt stress, etr1b, etr1a, and etr2b germinate earlier than WT, and the root and shoot growth rates of both seedlings and plants were less affected in mutant than in WT. The enhanced salt tolerance response of the etr2b plants was associated with a reduced accumulation of Na+ in shoots and leaves, as well as with a higher accumulation of compatible solutes, including proline and total carbohydrates, and antioxidant compounds, such as anthocyanin. Many membrane monovalent cation transporters, including Na+/H+ and K+/H+ exchangers (NHXs), K+ efflux antiporters (KEAs), high-affinity K+ transporters (HKTs), and K+ uptake transporters (KUPs) were also highly upregulated by salt in etr2b in comparison with WT. In aggregate, these data indicate that the enhanced salt tolerance of the mutant is led by the induction of genes that exclude Na+ in photosynthetic organs, while maintaining K+/Na+ homoeostasis and osmotic adjustment. If the salt response of etr mutants occurs via the ethylene signalling pathway, our data show that ethylene is a negative regulator of salt tolerance during germination and vegetative growth. Nevertheless, the higher upregulation of genes involved in Ca2+ signalling (CpCRCK2A and CpCRCK2B) and ABA biosynthesis (CpNCED3A and CpNCED3B) in etr2b leaves under salt stress likely indicates that the function of ethylene receptors in salt stress response in C. pepo can be mediated by Ca2+ and ABA signalling pathways.

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Faculty Opinions recommendation of The structure of the Arabidopsis thaliana SOS3: molecular mechanism of sensing calcium for salt stress response.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1024162.286767 ◽

2005 ◽

Author(s):

Ramón Serrano

Keyword(s):

Arabidopsis Thaliana ◽

Salt Stress ◽

Stress Response ◽

Molecular Mechanism ◽

Salt Stress Response

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Progress in understanding salt stress response in plants using biotechnological tools

Journal of Biotechnology ◽

10.1016/j.jbiotec.2021.02.007 ◽

2021 ◽

Vol 329 ◽

pp. 180-191

Author(s):

Ulkar İbrahimova ◽

Pragati Kumari ◽

Saurabh Yadav ◽

Anshu Rastogi ◽

Michal Antala ◽

...

Keyword(s):

Salt Stress ◽

Stress Response ◽

Salt Stress Response

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Proteome dynamics and early salt stress response of the photosynthetic organism Chlamydomonas reinhardtii

BMC Genomics ◽

10.1186/1471-2164-13-215 ◽

2012 ◽

Vol 13 (1) ◽

pp. 215 ◽

Cited By ~ 51

Author(s):

Guido Mastrobuoni ◽

Susann Irgang ◽

Matthias Pietzke ◽

Heike E Aßmus ◽

Markus Wenzel ◽

...

Keyword(s):

Salt Stress ◽

Stress Response ◽

Chlamydomonas Reinhardtii ◽

Salt Stress Response ◽

Photosynthetic Organism

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Interaction of SOS2 with Nucleoside Diphosphate Kinase 2 and Catalases Reveals a Point of Connection between Salt Stress and H2O2 Signaling in Arabidopsis thaliana

Molecular and Cellular Biology ◽

10.1128/mcb.00429-07 ◽

2007 ◽

Vol 27 (22) ◽

pp. 7771-7780 ◽

Cited By ~ 122

Author(s):

Paul E. Verslues ◽

Giorgia Batelli ◽

Stefania Grillo ◽

Fernanda Agius ◽

Yong-Sig Kim ◽

...

Keyword(s):

Salt Stress ◽

Stress Response ◽

Stress Responses ◽

Double Mutant ◽

Nucleoside Diphosphate Kinase ◽

Salt Resistance ◽

Interacting Proteins ◽

Salt Stress Response ◽

Oxygen Signaling ◽

Nucleoside Diphosphate Kinase 2

ABSTRACT SOS2, a class 3 sucrose-nonfermenting 1-related kinase, has emerged as an important mediator of salt stress response and stress signaling through its interactions with proteins involved in membrane transport and in regulation of stress responses. We have identified additional SOS2-interacting proteins that suggest a connection between SOS2 and reactive oxygen signaling. SOS2 was found to interact with the H2O2 signaling protein nucleoside diphosphate kinase 2 (NDPK2) and to inhibit its autophosphorylation activity. A sos2-2 ndpk2 double mutant was more salt sensitive than a sos2-2 single mutant, suggesting that NDPK2 and H2O2 are involved in salt resistance. However, the double mutant did not hyperaccumulate H2O2 in response to salt stress, suggesting that it is altered signaling rather than H2O2 toxicity alone that is responsible for the increased salt sensitivity of the sos2-2 ndpk2 double mutant. SOS2 was also found to interact with catalase 2 (CAT2) and CAT3, further connecting SOS2 to H2O2 metabolism and signaling. The interaction of SOS2 with both NDPK2 and CATs reveals a point of cross talk between salt stress response and other signaling factors including H2O2.

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