Increase in transcript accumulation of Psy1 and e-Lcy genes in grain development is associated with differences in seed carotenoid content between durum wheat and tritordeum

2013 ◽  
Vol 84 (6) ◽  
pp. 659-673 ◽  
Author(s):  
Cristina Rodríguez-Suárez ◽  
Elena Mellado-Ortega ◽  
Dámaso Hornero-Méndez ◽  
Sergio G. Atienza
2020 ◽  
Vol 11 ◽  
Author(s):  
María Dolores Requena-Ramírez ◽  
Sergio G. Atienza ◽  
Dámaso Hornero-Méndez ◽  
Cristina Rodríguez-Suárez

Carotenoids are essential in human diet, so that the development of programs toward carotenoid enhancement has been promoted in several crops. The cereal tritordeum, the amphiploid derived from the cross between Hordeum chilense Roem. et Schulz. and durum wheat has a remarkable carotenoid content in the endosperm. Besides, a high proportion of these carotenoids are esterified with fatty acids. The identification of the gene(s) responsible for xanthophyll esterification would be useful for breeding as esterified carotenoids show an increased ability to accumulate within plant cells and have a higher stability during post-harvest storage. In this work, we analyzed five genes identified as candidates for coding the xanthophyll acyltransferase (XAT) enzyme responsible for lutein esterification in H. chilense genome. All these genes were expressed during grain development in tritordeum, but only HORCH7HG021460 was highly upregulated. Sequence analysis of HORCH7HG021460 revealed a G-to-T transversion, causing a Glycine to Cysteine substitution in the protein of H290 (the only accession not producing quantifiable amounts of lutein esters, hereinafter referred as zero-ester) of H. chilense compared to the esterifying genotypes. An allele-specific marker was designed for the SNP detection in the H. chilense diversity panel. From the 93 accessions, only H290 showed the T allele and the zero-ester phenotype. Furthermore, HORCH7HG021460 is the orthologue of XAT-7D, which encodes a XAT enzyme responsible for carotenoid esterification in wheat. Thus, HORCH7HG021460 (XAT-7Hch) is a strong candidate for lutein esterification in H. chilense and tritordeum, suggesting a common mechanism of carotenoid esterification in Triticeae species. The transference of XAT-7Hch to wheat may be useful for the enhancement of lutein esters in biofortification programs.


2020 ◽  
Vol 21 (13) ◽  
pp. 4669 ◽  
Author(s):  
Roberto Parada ◽  
Conxita Royo ◽  
Agata Gadaleta ◽  
Pasqualina Colasuonno ◽  
Ilaria Marcotuli ◽  
...  

Phytoene synthase 1 (Psy1) and lipoxygenase 1 (Lpx-1) are key genes involved in the synthesis and catalysis of carotenoid pigments in durum wheat, regulating the increase and decrease in these compounds, respectively, resulting in the distinct yellow color of semolina and pasta. Here, we reported new haplotype variants and/or allele combinations of these two genes significantly affecting yellow pigment content in grain and semolina through their effect on carotenoid pigments. To reach the purpose of this work, three complementary approaches were undertaken: the identification of QTLs associated to carotenoid content on a recombinant inbred line (RIL) population, the characterization of a Mediterranean panel of accessions for Psy1 and Lpx-1 genes, and monitoring the expression of Psy1 and Lpx-1 genes during grain filling on two genotypes with contrasting yellow pigments. Our data suggest that Psy1 plays a major role during grain development, contributing to semolina yellowness, and Lpx-1 appears to be more predominant at post-harvest stages and during pasta making.


2017 ◽  
Vol 65 (15) ◽  
pp. 3242-3250 ◽  
Author(s):  
Maria Fiorella Mazzeo ◽  
Luigia Di Stasio ◽  
Chiara D’Ambrosio ◽  
Simona Arena ◽  
Andrea Scaloni ◽  
...  

2020 ◽  
pp. 26-31
Author(s):  
N. S. Kravchenko ◽  
N. E. Samofalova ◽  
I. M. Oldyreva ◽  
T. S. Makarova

The current paper has presented the study results of grain quality and pasta properties of the new and promising winter durum wheat varieties developed in the FSBSI “Agricultural Research Center “Donskoy” in 2016–2018. The purpose of the study was to evaluate winter durum wheat varieties according to such traits as grain quality and pasta properties to identify the best genotypes and recommend them for use in the breeding process and wider introduction into production. There have been identified the varieties “Yubilyarka” (96%), “Kristella” (94%), “Lakomka” (92%), “Diona” and “Yantarina” (91%) with maximum values of general hardness. All studied genotypes have formed the nature weight of grain corresponding to a first quality class (from 786–814 g/l). There have been identified the varieties “Kristella” (13.9%), “Donchanka” (13.8%), “Diona” (14.0%), “Yakhont” (14.0%), “Yubilyarka” (13.6%), “Yantarina” (13.7%), “Uslada” (13.6%), “Zoloto Dona” (13.6%), “Solntsedar” (13.9%) and “Almaz Dona” (13.5%) with protein percentage in grain corresponding to a first quality class. As a result of the study, it was found that all the studied varieties had good rheological properties of gluten and formed a DCI from 80 units (the variety “Eyrena”) to 102 units (the variety “Donchanka”) and belonged to the 2-nd group. The varieties “Diona”, “Eyrena”, “Yakhont”, “Yantarina” and “Solntsedar” had high rheological properties of dough. There have been identified the genotypes of the varieties “Donchanka” (613 μg/%), “Solntsedar” (626 μg/%) and “Almaz Dona” (630 μg/%) with the maximum concentration of carotenoid pigments, which could be used as sources to improve the trait “carotenoid content”. Throughout the years of study, the varieties were characterized by high indices of dry pasta color (4–5 points). According to a brittle strength of dry pasta there have been identified the varieties “Kristella” (775 g), “Donchanka” (774 g), “Diona” (757 g), “Yantarina” (782 g), “Solntsedar” (766 g) and “Dinas” (769 g) with the best indices of the trait. The varieties identified both by a single and by a set of traits characterizing grain quality of winter durum wheat and its final products, can be used in the breeding process as an adaptive initial material. The introduction of the best varieties into production will partly solve the problem of deficiency of high-quality durum wheat in the south of Russia.


Food systems ◽  
2020 ◽  
Vol 3 (2) ◽  
pp. 24-28
Author(s):  
T. S. Steinberg ◽  
E. P. Meleshkina ◽  
O. G. Shvedova ◽  
O. V. Morozova ◽  
N. S. Zhiltsova

Using the rapid method of digital image analysis by the developed scanning flour analyzer the optical properties of flour (semolina) obtained in laboratory and production conditions from durum wheat of three years of harvest (2017–2019), namely the «yellowness» indicator and the color characteristic in the blue part of the spectrum, were determined. The semolina color was also evaluated by the Konica Minolta CR-410 colorimeter. It is established that the «yellowness» indicator and the color characteristic in the blue part of the spectrum did not change within the first 5 to 6 days after grinding. The change of these indicators for all the samples is observed in the period from 6 to 20 days after grinding, there with the «yellowness» indicator decreased by 25 to 40 relative units, the color characteristic in the blue part of the spectrum increased on average by 133,75 relative units. Over the next three months, there was no change of color (by both indicators). In the course of experimental work the optical properties change depending upon carotenoids content of flour (semolina) during its ripening was shown. The correlation dependence between the «yellowness» indicator of flour (semolina) and its carotenoids content is characterized by a high approximation coefficient. The dependence of the color characteristic of flour in the blue part of the spectrum on the content of carotenoids is characterized by an approximation coefficient equal to 0.9358, and is described as a polynomial equation. It shows, that with a low carotenoid content, the considered indicator is higher by an average of 1100 relative units compared to the color of samples with a carotenoid content from 0.70 to 1.21 mcg/g. At that during storage the optical properties of flour variety with the lowest carotenoids content remained practically the same. During 78 days of storage, there was no significant change of color characteristics of the industrial flour samples, studied from the eleventh day after grinding — 5–8 times higher than the average repeatability of the measurement results.


2021 ◽  
Vol 97 ◽  
pp. 103131
Author(s):  
Adele Cutignano ◽  
Gianfranco Mamone ◽  
Floriana Boscaino ◽  
Aldo Ceriotti ◽  
Marco Maccaferri ◽  
...  

Agronomy ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1516
Author(s):  
Ana María Méndez-Espinoza ◽  
Miguel Garriga ◽  
Sinda Ben Mariem ◽  
David Soba ◽  
Iker Aranjuelo ◽  
...  

Grain development in cereals depends on synthesis and remobilisation compounds such as water-soluble carbohydrates (WSCs), amino acids (AAs), minerals and environmental conditions during pre- and post-anthesis. This study analyses the impact of water stress on metabolite (WSCs, AAs and nitrogen) dynamics between the source (leaves and stems) and sink (grain) organs in triticale, bread wheat, durum wheat and barley. Plants were grown in glasshouse conditions under well-watered (WW) and water-limited (WL) regimes (from flag leaf fully expanded until maturity). The results showed that the stem WSC content and the apparent mobilisation of WSC to the grain were much higher in triticale and were associated with its larger grain size and grain number. In the four cereals, grain weight and the number of kernels per spike were positively associated with stem WSC mobilisation. After anthesis, the AA concentration in leaves was much lower than in the grain. In grain, the main AAs in terms of concentration were Asn, Pro and Gln in triticale, bread, and durum wheat, and Asn, Pro and Val in barley. The water-limited regime reduced grain weight per plant in the four cereal species, but it had no clear effects on WSC content and AAs in leaves and grain. In general, triticale was less affected by WL than the other cereals.


2007 ◽  
Vol 58 (8) ◽  
pp. 767 ◽  
Author(s):  
S. G. Atienza ◽  
C. M. Avila ◽  
A. Martín

Hexaploid tritordeums are the amphiploids derived from the cross between the wild barley Hordeum chilense and durum wheat. Tritordeums are characterised by higher yellow pigment content in their seeds than their durum wheat progenitors due to certain H. chilense genes located on the α arm of chromosome 7Hch. In this work a candidate gene approach based on the phytoene synthase gene (PSY) was followed to investigate whether PSY1 may be responsible for the high carotenoid content in tritordeum and to develop a diagnostic marker for H. chilense PSY. This gene codes for the first step in the carotenoid biosynthetic pathway. It was first demonstrated that PSY is duplicated in H. chilense, Triticum urartu, and durum wheat (PSY1 and PSY2), and subsequently a diagnostic cleaved amplified polymorphism (CAP) marker able to differentiate between H. chilense and durum wheat PSY1 was developed. Using this CAP marker and a set of H. chilense-common wheat addition lines it was found that PSY1 is located on the α arm of chromosome 7Hch, where the gene(s) for yellow pigment content are located. PSY1 is located on chromosomes 7A and 7B of durum wheat as demonstrated using Langdon substitution lines. Furthermore, synteny between rice and wheat indicates that PSY1 should be located on the long arms of chromosomes 7A and 7B, in agreement with QTL data for yellow pigment content. Together, these results suggest that PSY1 may be a good candidate gene for further work with yellow pigment content in both durum wheat and tritordeum. In addition, the diagnostic CAP marker developed will be used in our breeding program to transfer H. chilense genes to durum wheat, to evaluate their potential for durum wheat improvement.


2010 ◽  
Vol 52 (1) ◽  
pp. 30-38 ◽  
Author(s):  
Adithya Ramachandran ◽  
Curtis J. Pozniak ◽  
John M. Clarke ◽  
Asheesh K. Singh

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