scholarly journals Plant tissue culture environment as a switch-key of (epi)genetic changes

2019 ◽  
Vol 140 (2) ◽  
pp. 245-257 ◽  
Author(s):  
Piotr Tomasz Bednarek ◽  
Renata Orłowska
Keyword(s):  
Tissue Culture ◽  
Plant Tissue ◽  
Basic Research ◽  
Current Status ◽  
Epigenetic Changes ◽  
Tissue Cultures ◽  
Genetic Changes ◽  
Culture Stage ◽  
Commercial Applications

Abstract The in vitro tissue cultures are, beyond all difficulties, an essential tool in basic research as well as in commercial applications. Numerous works devoted to plant tissue cultures proved how important this part of the plant science is. Despite half a century of research on the issue of obtaining plants in in vitro cultures, many aspects remain unknown. The path associated with the reprogramming of explants in the fully functioning regenerants includes a series of processes that may result in the appearance of morphological, physiological, biochemical or, finally, genetic and epigenetic changes. All these changes occurring at the tissue culture stage and appearing in regenerants as tissue culture-induced variation and then inherited by generative progeny as somaclonal variation may be the result of oxidative stress, which works at the step of explant preparation, and in tissue culture as a result of nutrient components and environmental factors. In this review, we describe the current status of understanding the genetic and epigenetic changes that occur during tissue culture.

Media derived from brown seaweeds Cystoseira myriophylloides and Fucus spiralis for in vitro plant tissue culture

2016 ◽  
Vol 128 (2) ◽  
pp. 437-446 ◽  
Author(s):  
Siham Esserti ◽  
Mohamed Faize ◽  
Lalla Aicha Rifai ◽  
Amal Smaili ◽  
Malika Belfaiza ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Brown Seaweeds ◽  
Fucus Spiralis ◽  
In Vitro Plant

In vitro tissue culture in breeding programs of leguminous pulses: use and current status

2016 ◽  
Vol 127 (3) ◽  
pp. 543-559 ◽  
Author(s):  
Ileana Gatti ◽  
Fernanda Guindón ◽  
Carolina Bermejo ◽  
Andrea Espósito ◽  
Enrique Cointry
Keyword(s):  
Tissue Culture ◽  
Current Status ◽  
Breeding Programs ◽  
In Vitro Tissue Culture

scholarly journals In vitro plant tissue culture: means for production of biological active compounds

Planta ◽  
2018 ◽  
Vol 248 (1) ◽  
pp. 1-18 ◽  
Author(s):  
Claudia A. Espinosa-Leal ◽  
César A. Puente-Garza ◽  
Silverio García-Lara
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Active Compounds ◽  
In Vitro Plant

scholarly journals Endogenous Bacterial Contamination of Plant Tissue Culture Materials: Identification and Control Strategy

2018 ◽  
Vol 28 (1) ◽  
pp. 99-108 ◽  
Author(s):  
Mohammad Ali ◽  
Shefali Boonerjee ◽  
Mohammad Nurul Islam ◽  
Mihir Lal Saha ◽  
M Imdadul Hoque ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Bacterial Contamination ◽  
Antibiotic Sensitivity ◽  
Sensitivity Test ◽  
Bacterial Isolates ◽  
Culture And Sensitivity ◽  
And Control

The endogenous bacterial contamination of plant tissue culture materials and their possible control was studied. Nine bacterial isolates were isolated from the contaminated tissue culture materials viz. potato and tea. On the basis of morphology and biochemical characters of nine isolates, seven were identified as Gram positive belonging to Bacillus alcalophilus, B. circulans, B. infantis, B. lentus, B. schlegelii, B. pumilus and B. subtilis. Remaining two were Gram negative and identified as Enterobacter cloacae sub. sp. dissolvens and Pantoea agglomerans. Molecular analysis was conducted on the basis of 16S rDNA sequence to confirm three isolates. Culture and sensitivity test was carried out to screen out the antibiotic sensitivity where streptomycin (S-10), polymyxin (PB-300) and gentamicin (CN-120) antibiotics were found to be effective against all bacterial isolates. The culture and sensitivity test reflected the feasibility to control or eliminate the contaminant bacteria during in vitro culture of plant which is very much required in the commercial tissue culture production.Plant Tissue Cult. & Biotech. 28(1): 99-108, 2018 (June)

Analysis of laser-induced fluorescence spectra of in vitro plant tissue cultures

2007 ◽  
Vol 46 (11) ◽  
pp. 2138 ◽  
Author(s):  
Ana Celia Muñoz-Muñoz ◽  
Humberto Gutiérrez-Pulido ◽  
José Manuel Rodríguez-Domínguez ◽  
Antonia Gutiérrez-Mora ◽  
Benjamín Rodríguez-Garay ◽  
...  
Keyword(s):  
Plant Tissue ◽  
Fluorescence Spectra ◽  
Laser Induced Fluorescence ◽  
Tissue Cultures ◽  
Plant Tissue Cultures ◽  
In Vitro Plant

Correlation of PCB Transformation by Plant Tissue Cultures with Their Morphology and Peroxidase Activity Changes

1999 ◽  
Vol 64 (9) ◽  
pp. 1497-1509 ◽  
Author(s):  
Petra Kučerová ◽  
Martina Macková ◽  
Ludmila Poláchová ◽  
Jiří Burkhard ◽  
Kateřina Demnerová ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Polychlorinated Biphenyls ◽  
Peroxidase Activity ◽  
Plant Tissue ◽  
Plant Cells ◽  
Tissue Cultures ◽  
Transforming Activity ◽  
Plant Tissue Cultures ◽  
Transformation Capacity

The ability of plant cells cultivated in vitro to metabolize polychlorinated biphenyls (PCBs) was correlated with the morphology of the cultures tested as models for phytoremediation studies. More differentiated cultures showed generally higher transformation capacity. The ability of plant cells to transform PCBs is connected to their viability in the presence of PCBs and their behaviour can be positively correlated with the production of intracellular and extracellular peroxidases. The cultures with high PCB-transforming activity proved to exhibit high peroxidase activity in the presence of PCBs while those with low ability to metabolize PCB showed a decrease of the enzyme activity in the presence of PCBs. Experiments with propylgallate were used to distinguish the ratio of involvement of peroxidases in PCB metabolism.

scholarly journals In vitro Degradation of 2,4,6-Trinitrotoluene Using Plant Tissue Cultures ofSolanum aviculare andRheum palmatum

2004 ◽  
Vol 4 (1) ◽  
pp. 46-49 ◽  
Author(s):  
A. Nepovím ◽  
M. Hubálek ◽  
R. Podlipná ◽  
S. Zeman ◽  
T. Vanek
Keyword(s):  
Plant Tissue ◽  
Tissue Cultures ◽  
In Vitro Degradation ◽  
Plant Tissue Cultures

Immune responses to hapten conjugates in vitro

1975 ◽  
Vol 8 (4) ◽  
pp. 507-522
Author(s):  
Sirkka Kontiainen ◽  
O. Mäkelä ◽  
M. Hurme
Keyword(s):  
Tissue Culture ◽  
Immune Responses ◽  
Cell Types ◽  
Antibody Responses ◽  
Tissue Cultures ◽  
Cell Type ◽  
Animal Body ◽  
Do So

Several functions of the animal body can take place in cell or tissue cultures with almost unreduced efficiency and precision. Functions, where only one cell type is involved, often do so, but also some differentiation steps where interactions between two or more cell types are clearly needed can take place in tissue culture (Saxén et al. 1968).Most immune responses require collaboration between two or more cell types (Claman, Chaperon & Triplett, 1966; Miller & Mitchell, 1968; Feldmann & Nossal 1972c). Some of them can be easily induced in vitro but others cannot. Even when antibody responses can be induced in vitro their intensity varies a great deal. With some antigens and under some circumstances a response in vitro can be nearly as strong as one in vivo. A crude comparison can be derived from responses in vitro and in vivo to the same antigen, conjugate of hapten NIP and pneumococcal polysaccharide type III (NIP-SIll, Nakamura, Ray & Mäkelä, 1973).

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