Centella asiatica L. callus mediated biosynthesis of silver nanoparticles, optimization using central composite design, and study on their antioxidant activity

Aptamer conjugated silver nanoparticles were synthesized for the colorimetric detection of As(iii) ions in solution. A central composite design combined with response surface methodology was employed to maximize the efficiency of sensing.

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Optimization The Effect of Roasting Conditions by Central Composite Design (CCD) Method on The Antioxidant Compounds of Opuntia Ficus Indica Seeds From Morocco

10.21203/rs.3.rs-31029/v1 ◽

2020 ◽

Author(s):

Chakir El Guezzane ◽

Hamza El Moudden ◽

Hicham Harhar ◽

Abdelkader Zarrouk ◽

Mohamed Tabyaoui

Keyword(s):

Antioxidant Activity ◽

Central Composite Design ◽

Principal Component ◽

Total Phenolic ◽

Antioxidant Compounds ◽

Opuntia Ficus Indica ◽

Optimal Value ◽

Total Flavonoids Content ◽

The One ◽

Central Composite

Abstract The effect of roasting conditions on antioxidant activity Opuntia Ficus Indica seeds from Morocco and their phenolic compounds were optimized by the Central Composite Design (CDD) method. The CCD was used to optimize the roasting conditions: temperature (X1: 60–200 °C) and roasting time (X2: 10–50 min). The best roasting conditions were used in order to optimize the optimal value of the response : TPC (Total Phenolic Content): X1: 200°C, X2: 50min with 104.86±1.94GAE/g extract predicted response, TFC (Total Flavonoids Content) : X1: 200 °C, X2: 50 min with 81.23±0.90 mg QE/g extract predicted responses, TTC (Total Condensed Tannins Content): X1: 128.9°C, X2: 34.92min with 6.12±0.95 mg QAE/g extract) predicted response. Moreover, the optimal potential antioxidant activity by ABTS assay and DPPH assay was found in extracts of Opuntia Ficus Indica seeds roasted: at a high temperature of the roasting. Furthermore, the positive significant correlations were determined by the principal component analysis (PCA) on the one hand, between the antioxidant capacity of the extracts and their antioxidants compounds (TPC and TFC), and on the other hand, between the two assays ABTS and DPPH. Consequently, the results of this work indicated that roasted Opuntia Ficus Indica seeds from Morocco can be considered as an essential ingredient for human foods.

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Optimization of supercritical fluid extraction of asiaticoside from Centella asiatica using Central Composite Design (CCD)

Separation Science and Technology ◽

10.1080/01496395.2020.1845208 ◽

2020 ◽

pp. 1-9

Author(s):

Fatin Shazwani Ruslan ◽

Deny Susanti ◽

Muhammad Taher ◽

Nurul Farahain Mohammad

Keyword(s):

Central Composite Design ◽

Supercritical Fluid Extraction ◽

Supercritical Fluid ◽

Centella Asiatica ◽

Fluid Extraction ◽

Central Composite

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Ultrasound-Assisted Phytochemical Extraction Condition Optimization Using Response Surface Methodology from Perlette Grapes (Vitis vinifera)

Processes ◽

10.3390/pr7100749 ◽

2019 ◽

Vol 7 (10) ◽

pp. 749 ◽

Cited By ~ 3

Author(s):

Muhammad Kaleem ◽

Asif Ahmad ◽

Rai Muhammad Amir ◽

Ghazala Kaukab Raja

Keyword(s):

Antioxidant Activity ◽

Response Surface Methodology ◽

Acetic Acid ◽

Vitis Vinifera ◽

Central Composite Design ◽

Flavonoid Content ◽

Ultrasound Assisted Extraction ◽

Assisted Extraction ◽

Ultrasound Assisted ◽

Central Composite

In the current study, bioactive compounds of Vitis vinifera (Perlette) were extracted using an ultrasound-assisted extraction technique. The central composite design of response surface methodology (RSM) was used to determine the effect of time, temperature, and concentration of acetic acid on response variables that include extract yield, total phenolic content (TPC), total flavonoid content (TFC), and antioxidant activity of Vitis vinifera extracts. The results of the central composite design of RSM revealed that the quadratic polynomial model is best fitted to experimental results, with all the responses having a regression coefficient greater than 0.9. Optimized extraction levels include 26.5 min, an extraction temperature of 59 °C, and an acetic acid concentration of 62.9% with good extraction yield results of 34.95 g/100 g dry weight (DW) of grapes, TPC 34.38 mg gallic acid equivalent per gram (GAE/g) DW, flavonoid content 10.21 mg quercetin equivalents per gram (QEQ/g) DW, and antioxidant activity of 9.11 mg/100 mL ascorbic acid equivalent. The present study showed that acetic acid can be effectively used for the ultrasound-assisted extraction of polyphenols, flavonoids, and antioxidants of Vitis vinifera. These optimized conditions can be used for the extraction of bioactive compounds that can be for the development of nutraceutical products.

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