Characterization and in vitro antibacterial activity of saponin-conjugated silver nanoparticles against bacteria that cause burn wound infection

2017 ◽  
Vol 33 (7) ◽  
Author(s):  
Anbarasan Muniyan ◽  
Kalaiselvi Ravi ◽  
Udayamathi Mohan ◽  
Rajasekar Panchamoorthy
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Wound Infection ◽  
Burn Wound ◽  
In Vitro Antibacterial Activity

scholarly journals The Design, Characterization and Antibacterial Activity of Heat and Silver Crosslinked Poly(Vinyl Alcohol) Hydrogel Forming Dressings Containing Silver Nanoparticles

Nanomaterials ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 96
Author(s):  
John Jackson ◽  
Helen Burt ◽  
Dirk Lange ◽  
In Whang ◽  
Robin Evans ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Silver Nitrate ◽  
Vinyl Alcohol ◽  
Unmet Need ◽  
Chemical Characterization ◽  
Wound Dressings ◽  
Poly Vinyl Alcohol ◽  
Burn Wound

The prompt treatment of burn wounds is essential but can be challenging in remote parts of Africa, where burns from open fires are a constant hazard for children and suitable medical care may be far away. Consequently, there is an unmet need for an economical burn wound dressing with a sustained antimicrobial activity that might be manufactured locally at low cost. This study describes and characterizes the novel preparation of a silver nitrate-loaded/poly(vinyl alcohol) (PVA) film. Using controlled heating cycles, films may be crosslinked with in situ silver nanoparticle production using only a low heat oven and little technical expertise. Our research demonstrated that heat-curing of PVA/silver nitrate films converted the silver to nanoparticles. These films swelled in water to form a robust, wound-compatible hydrogel which exhibited controlled release of the antibacterial silver nanoparticles. An optimal formulation was obtained using 5% (w/w) silver nitrate in PVA membrane films that had been heated at 140 °C for 90 min. Physical and chemical characterization of such films was complemented by in vitro studies that confirmed the effective antibacterial activity of the released silver nanoparticles against both gram positive and negative bacteria. Overall, these findings provide economical and simple methods to manufacture stable, hydrogel forming wound dressings that release antibiotic silver over prolonged periods suitable for emergency use in remote locations.

Surface Modification of Electrospun Polyethylenimine/Polyvinyl Alcohol Nanofibers Immobilized with Silver Nanoparticles for Potential Antibacterial Applications

2020 ◽  
Vol 16 ◽  
Author(s):  
Xiangyang Shi ◽  
Pengchao Liu ◽  
Yunpeng Huang ◽  
Xu Fang ◽  
Hui Ma ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Polyvinyl Alcohol ◽  
Electrospun Nanofibers ◽  
Cell Viability Assay ◽  
High Antibacterial Activity ◽  
Viability Assay ◽  
In Vitro Antibacterial Activity ◽  
Biological Protection

Objective: In order to investigate the potential biomedical applications of silver nanoparticle (Ag NP)-immobilized electrospun nanofibers with different surface functionalities. <P> Methods: silver nanoparticles were immobilized within water-stable electrospun polyethylenimine (PEI)/polyvinyl alcohol (PVA) nanofibers by an in-situ reduction method after complexing Ag+ ions with the free PEI amine groups. The obtained Ag NP-incorporated PEI/PVA nanofibers were then hydroxylated, carboxylated, and acetylated to generate different surface functionalities. Different techniques were employed to characterize the Ag NP-containing nanofibers with different surface functionalities. <P> Results: In vitro antibacterial activity tests show that Ag NP-containing nanofibrous mats have a high antibacterial activity and are capable of inhibiting the growth of both S. aureus and E. coli bacteria. Cell viability assay data show that the Ag NP-containing nanofibers are cytocompatible, and those treated by hydroxylation and acetylation display better cytocompatibility than those treated by carboxylation and the pristine non-modified fibers to promote cell adhesion and proliferation. <P> Conclusion: Therefore, the hydroxylated or acetylated Ag NP-containing PEI/PVA nanofibers have a great potential for wound dressing, biological protection and tissue engineering applications

scholarly journals Synthesis of Chitosan Stabilized Silver Nanoparticles and Evaluation of the in vitro Antibacterial Activity Against Xanthomonas oryzae pv. oryzae Causing Blight Disease of Rice

2021 ◽  
Author(s):  
Le Thi Hien ◽  
Nguyen Thi Phuong Hue ◽  
Le Trong Duc ◽  
Vu Thi Huyen ◽  
Le Thi Van ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Silver Nitrate ◽  
Bacterial Blight ◽  
Xanthomonas Oryzae ◽  
Diffusion Method ◽  
Colloidal Systems ◽  
Potential Measurement ◽  
In Vitro Antibacterial Activity

Bacterial blight is one of the most devastating rice diseases that cause huge economic loss worldwide. The cause of rice blight is Gram negative bacteria Xanthomonas oryzae pv. oryzae (X. oryzae pv. Oryzae). Since both silver nanoparticles and chitosan have antibacterial, antifungal and growth-stimulating effect, this work has focused on synthesizing chitosan stabilized silver nanoparticles (AgCSs) with small sizes and in vitro evaluating antibacterial activity against X. oryzae pv. oryzae bacteria. AgCSs were chemically synthesized by reducing silver nitrate by borohydride sodium in the presence of chitosan with optimization of the concentration of the reactants. AgCSs were characterized by UV/vis absorption spectra, field emission scanning electronic microscopy (FESEM), ImageJ software, zeta potential measurement, Fourier-transform infrared spectroscopy (FTIR) and X-ray diffraction. AgCSs have spherical configuration and narrow size distributions with different average sizes from 15 nm to 25 nm depending on the initial concentration of silver nitrate. All AgCSs colloidal systems were stable and exhibited no tendency for coagulation more than 5 months. It was the first time that chitosan-stabilized silver nanoparticles were assessed the in vitro antibacterial activity against bacterial blight VXO_281 strain. The disc diffusion method demonstrated that the smallest size silver nanoparticles (AgCS1) showed high antibacterial effect against the X. oryzae pv. oryzae VXO_281 strain with a concentration of more than 5 µg/mL and the inhibition zone was dose-dependent. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of AgCS1 against X. oryzae pv. oryzae VXO_281 were 2.5 µg/mL and 20 µg/mL, respectively.

scholarly journals Antibacterial Activity of Honey against Methicillin-Resistant Staphylococcus aureus: A Laboratory-Based Experimental Study

2019 ◽  
Vol 2019 ◽  
pp. 1-9 ◽  
Author(s):  
Mohammedaman Mama ◽  
Teklu Teshome ◽  
Jafer Detamo
Keyword(s):  
Staphylococcus Aureus ◽  
Experimental Study ◽  
Antibacterial Activity ◽  
Wound Infection ◽  
Antimicrobial Agents ◽  
Methicillin Resistance ◽  
Methicillin Resistant ◽  
Antimicrobial Drug Resistance ◽  
In Vitro Antibacterial Activity

Background. Antimicrobial drug resistance is one of the serious issues this world is facing nowadays, and increased cost of searching for effective antimicrobial agents and the decreased rate of new drug discovery have made the situation increasingly worrisome. Objective. The aim of this study is to determine in vitro antibacterial activity of honey against methicillin-resistant Staphylococcus aureus isolates from wound infection. Methods. An experimental study was conducted from May to November 2017. Methicillin resistance was detected using cefoxitin (30 μg) and oxacillin (1 μg) antibiotic discs. Different concentrations of honey (25–100% v/v) were tested against each type of clinical isolates obtained from wound infection. A preliminary sensitivity test was done to all types of honey by using disk diffusion while minimum inhibitory concentration and minimum bactericidal concentration were determined for the most potent honey by the broth dilution technique. All statistical analysis was performed by using Statistical Package for the Social Sciences version 20. Results. In this study, 36 bacterial isolates were recovered from 50 specimens, showing an isolation rate of 72%. The predominant bacteria isolated from the infected wounds were Staphylococcus aureus (15, 41.7%). Among identified Staphylococcus aureus, methicillin resistance accounts for 10 isolates (27.8%). All isolates showed a high frequency of resistance to tetracycline. Four collected honey varieties exhibited antibacterial activity, while the strongest inhibitory activity was demonstrated by honey-2 at 75% v/v. The mean MIC and MBC of honey-2 ranged from 9.38 to 37.5% v/v. Conclusions. Tested honey has both a bacteriostatic and bactericidal activity. Among the tested honey, “honey-2” had high antibacterial potency than others.

scholarly journals BIO-FABRICATION OF SILVER NANOPARTICLES USING CHRYSANTHEMUM CORONARIUM FLOWER EXTRACT AND IT’S IN VITRO ANTIBACTERIAL ACTIVITY

2018 ◽  
Vol 10 (5) ◽  
pp. 209
Author(s):  
Shyla M. Haqq ◽  
Himanshu Pandey ◽  
Manju Gerard ◽  
Amit Chattree
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Gram Negative Bacteria ◽  
Zone Of Inhibition ◽  
Gram Negative ◽  
E Coli ◽  
Flower Extract ◽  
Chrysanthemum Coronarium ◽  
In Vitro Antibacterial Activity

Objective: In the present research work silver nanoparticles were synthesized using the flower extract of Chrysanthemum coronarium and their in-vitro antibacterial activity was evaluated against both the gram-positive S. aureus and gram-negative bacteria E. coli. The flower extract acted both as a reducing as well as a capping agent.Methods: Silver nanoparticles were verified using various spectroanalytical techniques such as visible ultraviolet spectroscopy, zeta potential, fourier transform infrared spectroscopy and particle size analyser. The antibacterial activity was evaluated against both the gram-positive bacteria S. aureus and gram-negative bacteria E. coli using the agar well diffusion method.Results: The silver nanoparticles synthesized were confirmed by the visual colour change. The ultraviolet, visible spectroscopy showed a surface plasmon resonance at 430 nm. Zetapotential was found to be around-15.6mV where the negative value indicated that the synthesized silver nanoparticles are stable. Fourier transform infrared spectroscopy showed the functional groups responsible for the stabilization of the nanoparticles. Particle size analyser showed that the size of the nanoparticles ranged from 5-50 nm. The antibacterial activity of the silver nanoparticles which was performed against S. aureus and E. coli showed good inhibition against both the bacteria. Better antibacterial activity was found for E. coli in comparison to S. aureus as the zone of inhibition for E. coli was found to be at 12 mm at 50ug/ml whereas the zone of inhibition against S. aureus was found to be at 10 mm.Conclusion: The silver nanoparticles were successfully synthesized using a green approach and can be used as a potential resource for therapeutic purpose.

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