Evolution of the microbial community of the biofilm in a methane-based membrane biofilm reactor reducing multiple electron acceptors

The back-diffusion of inactive gases severely inhibits the hydrogen (H2) delivery rate of the close-end operated hydrogen-based membrane biofilm reactor (H2-based MBfR). Nevertheless, less is known about the response of microbial communities in H2-based MBfR to the impact of the gases’ back-diffusion. In this research, the denitrification performance and microbial dynamics were studied in a H2-based MBfR operated at close-end mode with a fixed H2 pressure of 0.04 MPa and fed with nitrate (NO3−) containing influent. Results of single-factor and microsensor measurement experiments indicate that the H2 availability was the decisive factor that limits NO3− removal at the influent NO3− concentration of 30 mg N/L. High-throughput sequencing results revealed that (1) the increase of NO3− loading from 10 to 20–30 mg N/L resulted in the shift of dominant functional bacteria from Dechloromonas to Hydrogenophaga in the biofilm; (2) excessive NO3− loading led to the declined relative abundance of Hydrogenophaga and basic metabolic pathways as well as counts of most denitrifying enzyme genes; and (3) in most cases, the decreased quantity of N metabolism-related functional bacteria and genes with increasing distance from the H2 supply end corroborates that the microbial community structure in H2-based MBfR was significantly impacted by the gases’ back-diffusion.

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Effect of UV on De-NOxperformance and microbial community of a hybrid catalytic membrane biofilm reactor

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/121/3/032024 ◽

2018 ◽

Vol 121 ◽

pp. 032024 ◽

Cited By ~ 1

Author(s):

Zhouyang Chen ◽

Zhensha Huang ◽

Yiming He ◽

Xiaoliang Xiao ◽

Zaishan Wei

Keyword(s):

Microbial Community ◽

Biofilm Reactor ◽

Catalytic Membrane ◽

Membrane Biofilm Reactor

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Insights into the removal of gaseous oxytetracycline by combined ozone and membrane biofilm reactor

Environmental Engineering Research ◽

10.4491/eer.2021.469 ◽

2021 ◽

Vol 27 (6) ◽

pp. 210469-0

Author(s):

Z.S. Wei ◽

X.L. Chen ◽

Z.S. Huang ◽

H.Y. Jiao ◽

X.L. Xiao

Keyword(s):

Molecular Structure ◽

High Temperature ◽

Microbial Community ◽

Cost Effective ◽

Biofilm Reactor ◽

Community Diversity ◽

Ecological Environment ◽

The Novel ◽

Waste Gas ◽

Membrane Biofilm Reactor

Gaseous emerging organic compounds (GEOCs) may harm human health and ecological environment. High temperature composting of livestock manure may produce oxytetracycline (OTC) waste gas. Here, we investigated treatment OTC in waste gas by combined ozone and membrane biofilm reactor (MBfR) with desulphurizing bacteria. The performance, the microbial community, gene function and the mechanism for OTC removal in the ozone-MBfR were evaluated. The ozone-MBfR system could achieve more degradation of OTC completely than MBfR. Desulfovibrio, Lentimicrobium, Aminivibrio, Thioalkalispira, Erysipelothrix, Mangroviflexus, Azoarcus, Thauera, Geobacter, Paracoccus, and Dethiosulfatibacter were the dominant genera. Pseudomonas, Escherichia, Bacteroides, Salmonella, Paracoccus, Stappia were contribution to OTC degradation. With the addition of ozone, the community diversity increased; some genera, such as Tenericutes- uncultured, and Desulfovibrio, increased in abundance, whereas others, such as Thauera, and Petrimonas, decreased. Ozone destroyed the enol structure in OTC molecular structure and produces biodegradable products, ozone oxidation was combined with biodegradation, to achieve thoroughly degrade OTC in waste gas. The novel hybrid ozone-MBfR is a cost-effective and robust alternative to GEOCs treatment.

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