Bioethanol production from defatted biomass of Nannochloropsis oculata microalgae grown under mixotrophic conditions

Abstract Microalgal biomass is one of the most promising third-generation feedstocks for bioethanol production because it contains significantly reduced sugar amounts which, by separate hydrolysis and fermentation, can be used as a source for ethanol production. In this study, the defatted microalgal biomass of Nannochloropsis oculata (NNO-1 UTEX Culture LB 2164) was subjected to bioethanol production through acid digestion and enzymatic treatment before being fermented by Saccharomyces cerevisiae (NRRLY-2034). For acid hydrolysis (AH), the highest carbohydrate yield 252.84 mg/g DW was obtained with 5.0% (v/v) H2SO4 at 121°C for 15 min for defatted biomass cultivated mixotrophically on SBAE with respect to 207.41 mg/g DW for defatted biomass cultivated autotrophically (control treatment), Whereas, the highest levels of reducing sugars was obtained With 4.0%(v/v) H2SO4 157.47 ± 1.60 mg/g DW for defatted biomass cultivated mixotrophically in compared with 135.30 mg/g DW for the defatted control treatment. The combination of acid hydrolysis 2.0% (v/v) H2SO4 followed by enzymatic treatment (AEH) increased the carbohydrate yields to 268.53 mg/g DW for defatted biomass cultivated mixotrophically on SBAE with respect to 177.73 mg/g DW for the defatted control treatment. However, the highest levels of reducing sugars were obtained with 3.0% (v/v) H2SO4 followed by enzyme treatment gave 232.39 ± 1.77 for defatted biomass cultivated mixotrophically on SBAE and 150.75 mg/g DW for the defatted control treatment. The sugar composition of the polysaccharides showed that glucose was the principal polysaccharide sugar (60.7%-62.49%) of N. oculata defatted biomass. Fermentation of the hydrolysates by Saccharomyces cerevisiae for the acid pretreated defatted biomass samples gave ethanol yield of 0.86 g/l (0.062 g/g sugar consumed) for control and 1.17 g/l (0.069 g/g sugar consumed) for SBAE mixotrophic. Whereas, the maximum ethanol yield of 6.17 ± 0.47 g/l (0.26 ± 0.11 g/g sugar consumed) was obtained with samples from defatted biomass grown mixotrophically (SBAE mixotrophic) pretreated with acid coupled enzyme hydrolysis.

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Batch cultivation of marine microalgae Nannochloropsis oculata and Tetraselmis suecica in treated municipal wastewater toward bioethanol production

Journal of Cleaner Production ◽

10.1016/j.jclepro.2017.02.189 ◽

2017 ◽

Vol 150 ◽

pp. 40-46 ◽

Cited By ~ 42

Author(s):

Zubaidai Reyimu ◽

Didem Özçimen

Keyword(s):

Municipal Wastewater ◽

Batch Cultivation ◽

Nannochloropsis Oculata ◽

Marine Microalgae ◽

Bioethanol Production ◽

Tetraselmis Suecica ◽

Treated Municipal Wastewater

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Construction Cost of KP Mill Construction for Bioethanol Production

JAPAN TAPPI JOURNAL ◽

10.2524/jtappij.69.518 ◽

2015 ◽

Vol 69 (5) ◽

pp. 518-531

Author(s):

Tokiya Yaguchi ◽

Makoto Iwasaki ◽

Youichiro Isono

Keyword(s):

Construction Cost ◽

Bioethanol Production

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Efektifitas Ekstrak Nannochloropsis oculata Terhadap Peningkatan Kepadatan Kolagen pada Proses Penyembuhan Alveolar Osteitis

DENTA ◽

10.30649/denta.v10i1.16 ◽

2016 ◽

Vol 10 (1) ◽

pp. 9

Author(s):

Ajeng Saghita Enggardini ◽

Syamsulina Revianti ◽

Noengki Prameswari

Keyword(s):

Nannochloropsis Oculata ◽

Control Group ◽

Control Group Design ◽

Group Design ◽

Post Test ◽

Alveolar Osteitis

Latar Belakang: Alveolar osteitis merupakan salah satu komplikasi pasca pencabutan gigi. Kondisi ini biasanya menjadi masalah pasca pencabutan yang menyebabkan rasa nyeri dan menghambat penyembuhan soket. Ekstrak Nannochloropsis oculata mengandung alkaloid, carotenoid, flavonoid, terpenoid dan tocopherol yang dapat mempercepat proses penyembuhan luka. Tujuan: Mengetahui efektivitas ekstrak Nannochloropsis oculata terhadap peningkatan kepadatan kolagen pada proses penyembuhan alveolar osteitis. Bahan dan Metode: Rancangan penelitian ini adalah post test only control group design, dengan menggunakan 64 tikus Wistar jantan dibagi menjadi 2 kelompok besar, yaitu pemberian irigasi dan dressing. Pada masing-masing kelompok terdapat kelompok kontrol negatif (irigasi PBS atau dressing PBS), kelompok kontrol positif (irigasi Saline 0,9% atau dressing Alvogyl) dan kelompok perlakuan (irigasi atau dressing ekstrak Nannochloropsis oculata 2,5% dan 5%). Kemudian kepadatan kolagen diukur pada hari ke 3 dan ke 7 setelah perlakuan. Kepadatan kolagen yang diukur pada soket pasca pencabutan gigi. Setelah dilakukan treatment pada hari ke 3 dan ke 7, semua tikus dikorbankan lalu kepadatan kolagen diukur melalui preparat histopatologi anatomi dengan pengecatan Masson’s Trichom dan pembesaran 100x, kemudian dilakukan perhitungan kepadatan kolagen. Data yang diperoleh dianalisis Kruskal-Wallis dan Mann-Whitney. Hasil: Terdapat perbedaan kepadatan kolagen yang signifikan antara kelompok perlakuan irigasi (0,045), tetapi tidak terdapat perbedaan yang signifikan antara kelompok perlakuan dressing (0,243). Metode dressing lebih efektif dalam meningkatkan kepadatan kolagen jika dibandingkan dengan kelompok irigasi (0,026). Simpulan: Pemberian dressing ekstrak Nannochloropsis oculata konsentrasi 5% paling efektif dalam meningkatkan kepadatan kolagen pada proses penyembuhan alveolar osteitis pada hari ke 7.

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Bioethanol production from lignocellulosic feedstock using aqueous ammonia pretreatment and simultaneous saccharification and fermentation (SSF): process development and optimization

10.31274/etd-180810-551 ◽

2010 ◽

Author(s):

Xuan Li

Keyword(s):

Simultaneous Saccharification And Fermentation ◽

Process Development ◽

Aqueous Ammonia ◽

Bioethanol Production ◽

Lignocellulosic Feedstock ◽

Ammonia Pretreatment ◽

Ssf Process ◽

Simultaneous Saccharification

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STUDY AND CHARACTERIZATION GROWTH OF FOUR MICROALGAE SPECIES AND TEST ANTIMICROBIAL ACTIVITY

Jurnal Zarah ◽

10.31629/zarah.v6i2.625 ◽

2018 ◽

Vol 6 (2) ◽

pp. 53-58

Author(s):

Marniati Salim

Keyword(s):

Dunaliella Salina ◽

Methanol Extract ◽

Sea Water ◽

Basal Medium ◽

Nannochloropsis Oculata ◽

Inhibitory Zone ◽

Antimicrobial Sensitivity ◽

Sensitivity Tests ◽

Microalgae Biomass ◽

Chaetoceros Calcitrans

Abstract In this study to the growth characteristics of microalgae (Dunaliella salina, Nannochloropsis oculata, Tetrasel mis chuii & Chaetoceros calcitrans), in different mediums, namely Bold’s Basal Medium (BBM) and BBM modification of sea water. The results obtained from microalgae (N.oculata, T.chuii, C.calcitrans) are better grown on BBM media while microlaga D.salina grows well on BBM modification of sea water. Microalgae biomass is extracted by maceration method in hexane and methanol solvents. Test bacteria used gram positive (Staphylococcus aureus) and gram negative (Escherichia coli). Inhibitory zone diameter results were compared with antimicrobial sensitivity tests according to Clinical and Laboratory Standards Institute (CLSI). From the results of microalgae hexane and methanol extract antibacterial activity tests (D.salina, N.oculata, T.chuii, C.calcitrans) showed that the extract was not too sensitive to inhibit bacterial growth. Keywords: microalgae, medium, antimicrobial

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Using Biogas From Vinasse To Enable Sugarcane Bagasse Availability For Bioethanol Production

10.31988/scitrends.16199 ◽

2018 ◽

Author(s):

Caio L. Joppert

Keyword(s):

Sugarcane Bagasse ◽

Bioethanol Production

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Isolation, morphometry, and culture of Colurella sp. (Rotifera: Ploimida)

AQUATIC SCIENCE & MANAGEMENT ◽

10.35800/jasm.1.2.2013.7276 ◽

2013 ◽

Vol 1 (2) ◽

pp. 143

Author(s):

Petrus P Letsoin ◽

Henneke Pangkey ◽

Julius Sampekalo ◽

Inneke F.M Rumengan ◽

Stenly Wullur ◽

...

Keyword(s):

Body Size ◽

Marine Fish ◽

Fish Larvae ◽

Nannochloropsis Oculata ◽

Tropical Fish ◽

Sampling Location ◽

Larval Rearing ◽

Brachionus Rotundiformis ◽

Marine Fish Larvae ◽

Significant Difference

The rotifer Brachionus rotundiformis (total body length 240.59±10.24 μm, lorica length 175.28±9.18 μm, and lorica width 124.28±7.76μm) is commonly used as starter food in the larval rearing of marine fish. But, larvae of some marine tropical fish species required starter food with body size smaller than B. rotundiformis. The present study was aimed to isolate minute rotifers from nature and to assess the possibility of culturing these rotifers. Sampling of rotifers was conducted in an estuary of Mangket (Kema-Minut), using plankton net (mesh size 40 µm). A trial of culturing the rotifers was conducted at salinities of 10, 20 and 30 ppt by using a microalga, Nannochloropsis oculata. A species of rotifer identified as Colurella sp. (family Lepadellidae) was successfully isolated from the sampling location. Body size of Colurella sp. was extremely small (Total length 123.22±5.45 μm, lorica length 95.96±3.81 μm, and lorica width 53.57±3.11 μm), which were smaller than Brachionus rotundiformis SS-type as a conventional starter food for marine fish larvae. Results of culturing the minute rotifer Colurella sp. showed that the species grew well at salinities of 10, 20 and 30 ppt with no significant difference among treatments (ANOVA, p>0.05), indicating a potential use of minute rotifer Colurellasp. as starter food for marine fish larvae. Rotifera Branchionus rotundiformis (ukuran tubuh: panjang total 240,59±10,24 μm, panjang lorika 175,28±9,18 μm, dan lebar lorika 124,28±7,76μm) sering digunakan sebagai pakan awal pemeliharaan larva ikan laut. Namun, larva beberapa spesis ikan laut tropis membutuhkan pakan awal berukuran tubuh lebih kecil dari Branchionus rotundiformis. Penelitian ini bertujuan untuk mendapatkan minute rotifer dari alam (berukuran tubuh lebih kecil dari B. rotundiformis) dan menguji kemungkinan pemeliharaannya. Sampling rotifer dilakukan di perairan estuari Desa Mangket (Kema-Minut), menggunakan plankton net (ukuran mata jaring 40 µm). Uji coba pemeliharaan dilakukan pada salinitas (10, 20, dan 30 ppt) dengan menggunakan Nannochloropsis oculata. Satu spesies minute rotifer yang teridentifikasi sebagai Colurella sp. (family Lepadellidae) berhasil diisolasi dari lokasi sampling. Colurella sp. memiliki ukuran tubuh sangat kecil (panjang total [PT] 123,22±5,45 µm, panjang lorika [PL] 95,96±3,81 µm, dan lebar lorik [LL] 53,57±3,11 µm) yang mana lebih kecil dari Branchionus rotundiformis tipe-SS sebagai pakan awal larva ikan laut. Hasil uji coba pemeliharaan minute rotifer Colurella sp. menunjukkan bahwa spesis ini dapat tumbuh pada salinitas 10, 20, dan 30 ppt dengan perbedaan kepadatan populasi yang tidak signifikan antar perlakuan (Uji ANOVA, p > 0.05) mengindikasikan potensi pemanfaatan minute rotifer Colurella sp. sebagai pakan awal larva ikan laut.

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