Anti-inflammatory activities of Ophiopogonis Radix on hydrogen peroxide-induced cellular senescence of normal human dermal fibroblasts

2018 ◽  
Vol 72 (4) ◽  
pp. 905-914 ◽  
Author(s):  
Yumi Kitahiro ◽  
Atsushi Koike ◽  
Aska Sonoki ◽  
Mei Muto ◽  
Kazuo Ozaki ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Cellular Senescence ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts ◽  
Normal Human ◽  
Anti Inflammatory ◽  
Normal Human Dermal Fibroblasts

scholarly journals Anti-Inflammatory Effect of ETAS®50 by Inhibiting Nuclear Factor-κB p65 Nuclear Import in Ultraviolet-B-Irradiated Normal Human Dermal Fibroblasts

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Ken Shirato ◽  
Tomoko Koda ◽  
Jun Takanari ◽  
Takuya Sakurai ◽  
Junetsu Ogasawara ◽  
...  
Keyword(s):  
Uv Irradiation ◽  
Nuclear Import ◽  
Dermal Fibroblasts ◽  
Nuclear Factor ◽  
Human Dermal Fibroblasts ◽  
Protein Levels ◽  
Proinflammatory Responses ◽  
Normal Human ◽  
Anti Inflammatory ◽  
Normal Human Dermal Fibroblasts

Ultraviolet (UV) irradiation induces proinflammatory responses in skin cells, including dermal fibroblasts, accelerating premature skin aging (photoaging). ETAS 50, a standardized extract from theAsparagus officinalisstem, is a novel and unique functional food that suppresses proinflammatory responses of hydrogen peroxide-stimulated skin fibroblasts and interleukin- (IL-) 1β-stimulated hepatocytes. To elucidate its antiphotoaging potencies, we examined whether ETAS 50 treatment after UV-B irradiation attenuates proinflammatory responses of normal human dermal fibroblasts (NHDFs). UV-B-irradiated NHDFs showed reduced levels of the cytosolic inhibitor of nuclear factor-κBα(IκBα) protein and increased levels of nuclear p65 protein. The nuclear factor-κB nuclear translocation inhibitor JSH-23 abolished UV-B irradiation-induced IL-1βmRNA expression, indicating that p65 regulates transcriptional induction. ETAS 50 also markedly suppressed UV-B irradiation-induced increases in IL-1βmRNA levels. Immunofluorescence analysis revealed that ETAS 50 retained p65 in the cytosol after UV-B irradiation. Western blotting also showed that ETAS 50 suppressed the UV-B irradiation-induced increases in nuclear p65 protein. Moreover, ETAS 50 clearly suppressed UV-B irradiation-induced distribution of importin-αprotein levels in the nucleus without recovering cytosolic IκBαprotein levels. These results suggest that ETAS 50 exerts anti-inflammatory effects on UV-B-irradiated NHDFs by suppressing the nuclear import machinery of p65. Therefore, ETAS 50 may prevent photoaging by suppressing UV irradiation-induced proinflammatory responses of dermal fibroblasts.

scholarly journals ETAS®50 Attenuates Ultraviolet-B-Induced Interleukin-6 Expression by Suppressing Akt Phosphorylation in Normal Human Dermal Fibroblasts

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Ken Shirato ◽  
Tomoko Koda ◽  
Jun Takanari ◽  
Junetsu Ogasawara ◽  
Takuya Sakurai ◽  
...  
Keyword(s):  
P38 Mapk ◽  
Dermal Fibroblasts ◽  
Ultraviolet B ◽  
Asparagus Officinalis ◽  
Mrna Levels ◽  
Human Dermal Fibroblasts ◽  
Akt Phosphorylation ◽  
Normal Human ◽  
Anti Inflammatory ◽  
Normal Human Dermal Fibroblasts

We recently reported that ETAS 50, a standardized extract from theAsparagus officinalisstem, exerted anti-inflammatory effects on ultraviolet-B- (UV-B-) irradiated normal human dermal fibroblasts (NHDFs) by inhibiting nuclear factor-κB p65 nuclear import and the resulting interleukin-1β(IL-1β) expression. To further elucidate the antiphotoaging potency of ETAS 50, we examined the anti-inflammatory effects on UV-B-irradiated NHDFs by focusing on the stress-activated mitogen-activated protein kinase (MAPK) and Akt signaling pathways. NHDFs were treated with 1 mg/mL of ETAS 50 or dextrin (vehicle control) after UV-B irradiation (20 mJ/cm2) for different time periods. Phosphorylation levels of c-Jun N-terminal kinase (JNK), p38 MAPK, and Akt were analyzed by western blotting. IL-6 mRNA levels were analyzed by real-time polymerase chain reaction. UV-B-irradiated NHDFs showed increased phosphorylation levels of JNK, p38 MAPK, and Akt, as well as increased mRNA levels of IL-6. ETAS 50 treatment after UV-B irradiation suppressed the increased phosphorylation levels of Akt without affecting those of JNK and p38 MAPK. ETAS 50 as well as Akt inhibitor Perifosine repressed UV-B irradiation-induced IL-6 mRNA expression. These results suggest that ETAS 50 treatment represses UV-B irradiation-induced IL-6 expression by suppressing Akt phosphorylation. The present findings demonstrate the potential of ETAS 50 to prevent photoaging by attenuating UV-B irradiation-induced proinflammatory responses in skin fibroblasts.

scholarly journals Endoplasmic reticulum resident proteins of normal human dermal fibroblasts are the major targets for oxidative stress induced by hydrogen peroxide

2002 ◽  
Vol 366 (3) ◽  
pp. 825-830 ◽  
Author(s):  
Dennis van der VLIES ◽  
Eward H.W. PAP ◽  
Jan Andries POST ◽  
Julio E. CELIS ◽  
Karel W.A. WIRTZ
Keyword(s):  
Hydrogen Peroxide ◽  
Endoplasmic Reticulum ◽  
Laser Scanning ◽  
Dermal Fibroblasts ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Human Dermal Fibroblasts ◽  
Normal Human ◽  
Covalently Linked ◽  
Normal Human Dermal Fibroblasts

The membrane-permeable fluorescein-labelled tyramine conjugate (acetylTyrFluo) was used to identify the proteins of normal human dermal fibroblasts most susceptible to oxidation by hydrogen peroxide [Van der Vlies, Wirtz and Pap (2001) Biochemistry 40, 7783—7788]. By exposing the cells to H2O2 (0.1mM for 10min), TyrFluo was covalently linked to target proteins. TyrFluo-labelled and [35S]Met-labelled cell lysates were mixed and subjected to two-dimensional PAGE. After Western blotting the 35S-labelled proteins were visualized by autoradiography and the TyrFluo-labelled proteins by using anti-fluorescein antibody. The TyrFluo-labelled proteins were matched with the 35S-labelled proteins and identified by comparison with our mastermap of proteins. Protein disulphide isomerase (PDI), IgG-binding protein (BiP), calnexin, endoplasmin and glucose-regulated protein 58 (endoplasmic reticulum protein 57/GRP58) were identified as targets of oxidation. All these proteins reside in the endoplasmic reticulum and are part of the protein folding machinery. In agreement, confocal laser scanning microscopy showed co-localization of TyrFluo-labelled proteins and the KDEL receptor ERD-2, a marker for the endoplasmic reticulum.

scholarly journals Effects of GABA on the expression of type I collagen gene in normal human dermal fibroblasts

2016 ◽  
Vol 81 (2) ◽  
pp. 376-379 ◽  
Author(s):  
Eriko Uehara ◽  
Hideki Hokazono ◽  
Takako Sasaki ◽  
Hidekatsu Yoshioka ◽  
Noritaka Matsuo
Keyword(s):  
Type I Collagen ◽  
Dermal Fibroblasts ◽  
Type I ◽  
Collagen Gene ◽  
Human Dermal Fibroblasts ◽  
Normal Human ◽  
Normal Human Dermal Fibroblasts
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