Role of EGFL7/EGFR-signaling pathway in migration and invasion of growth hormone-producing pituitary adenomas

ABSTRACTOBJECTIVETo investigate the effect of the miR-195/EGFR signaling pathway on trophoblasts in patients with recurrent spontaneous abortion, thus providing a clinical basis for the diagnosis and treatment of recurrent spontaneous abortion.METHODSRT-qPCR, western blot, flow cytometry, CCK8, cell scratch assay, transwell, and a dual Luciferase reporter assay were used to detect changes in the miR-195/EGFR signaling pathways in clinical samples and in vitro cultured cells and to explore how these changes affect trophoblasts in affected patients.RESULTSExpression of miR-195 was elevated in villus tissues of patients with recurrent spontaneous abortion, while the expression levels of EGFR and its downstream genes p38 and AKT phosphorylation were down-regulated. In vitro cultured cell experiments showed that miR-195 inhibited the proliferation, migration, and invasion of trophoblast cells. EGFR is a target gene of miR-195, and miR-195 suppresses the expression of EGFR.ConclusionThe miR-195/EGFR signaling pathway regulates the proliferation, migration, and invasion of trophoblast cells, thus playing an important role in recurrent spontaneous abortion.

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Combinatorial role of the EGFR signaling pathway and a RUNX family transcription factor Lozenge in the subtype specification of the Drosophila olfactory sensory neurons

Neuroscience Research ◽

10.1016/j.neures.2009.09.384 ◽

2009 ◽

Vol 65 ◽

pp. S92

Author(s):

Keita Endo ◽

Shohei Kuwa ◽

Kei Ito

Keyword(s):

Transcription Factor ◽

Signaling Pathway ◽

Sensory Neurons ◽

Olfactory Sensory Neurons ◽

Egfr Signaling ◽

Egfr Signaling Pathway

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Triptolide inhibits pituitary adenoma cell viability, migration and invasion via ADAM12/EGFR signaling pathway

Life Sciences ◽

10.1016/j.lfs.2017.12.037 ◽

2018 ◽

Vol 194 ◽

pp. 150-156 ◽

Cited By ~ 4

Author(s):

Junwen Wang ◽

Zhuo Zhang ◽

Ran Li ◽

Wei Sun ◽

Juan Chen ◽

...

Keyword(s):

Pituitary Adenoma ◽

Cell Viability ◽

Signaling Pathway ◽

Migration And Invasion ◽

Egfr Signaling ◽

Adenoma Cell ◽

Egfr Signaling Pathway

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MD2 blockage prevents the migration and invasion of hepatocellular carcinoma cells via inhibition of the EGFR signaling pathway

Journal of Gastrointestinal Oncology ◽

10.21037/jgo-21-362 ◽

2021 ◽

Vol 0 (0) ◽

pp. 0-0

Author(s):

Yajun Qi ◽

Qilu Fang ◽

Qinglin Li ◽

Haiying Ding ◽

Qi Shu ◽

...

Keyword(s):

Hepatocellular Carcinoma ◽

Signaling Pathway ◽

Carcinoma Cells ◽

Migration And Invasion ◽

Egfr Signaling ◽

Hepatocellular Carcinoma Cells ◽

Egfr Signaling Pathway

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Ergosta-7,22-diene-2β,3α,9α-triol (EGDT) from Ganoderma lucidum inhibits nasopharyngeal carcinoma cells by blocking EGFR signaling pathway

Chinese Herbal Medicines ◽

10.1016/j.chmed.2017.12.003 ◽

2018 ◽

Vol 10 (1) ◽

pp. 27-33

Author(s):

Wen-Wei Wu ◽

Zi-Jing Ye ◽

Yi-Mou Xu ◽

Jin Zhang ◽

Jin-Sen Tang

Keyword(s):

Nasopharyngeal Carcinoma ◽

Signaling Pathway ◽

Ganoderma Lucidum ◽

Carcinoma Cells ◽

Egfr Signaling ◽

Egfr Signaling Pathway

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Association of different pathologic subtypes of growth hormone producing pituitary adenoma and remission in acromegaly patients: a retrospective cohort study

BMC Endocrine Disorders ◽

10.1186/s12902-021-00850-2 ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Maryam Dehghani ◽

Zahra Davoodi ◽

Farahnaz Bidari ◽

Amin Momeni Moghaddam ◽

Davood Khalili ◽

...

Keyword(s):

Growth Hormone ◽

Cohort Study ◽

Cavernous Sinus ◽

Retrospective Cohort Study ◽

Pituitary Adenomas ◽

Retrospective Cohort ◽

Remission Rate ◽

Cavernous Sinus Invasion ◽

Dual Staining

Abstract Background Regarding the inconclusive results of previous investigations, this study aimed to determine the association between pathology, as a possible predictor, with remission outcomes, to know the role of pathology in the personalized decision making in acromegaly patients. Methods A retrospective cohort study was performed on the consecutive surgeries for growth hormone (GH) producing pituitary adenomas from February 2015 to January 2021. Seventy-one patients were assessed for granulation patterns and prolactin co-expression as dual staining adenomas. The role of pathology and some other predictors on surgical remission was evaluated using logistic regression models. Results Among 71 included patients, 34 (47.9%) patients had densely granulated (DG), 14 (19.7%) had sparsely granulated (SG), 23 (32.4%) had dual staining pituitary adenomas. The remission rate was about 62.5% in the patients with SG and DG adenomas named single staining and 52.2% in dual staining groups. Postoperative remission was 1.53-folds higher in the single staining adenomas than dual staining-one (non-significant). The remission rate was doubled in DG group compared to two other groups (non-significant). By adjusting different predictors, cavernous sinus invasion and one-day postoperative GH levels decreased remission rate by 91% (95% CI: 0.01–0.67; p = 0.015) and 64% (95% CI: 0.19–0.69; p < 0.001), respectively. Responses to the medications were not significantly different among three groups. Conclusion Various pathological subtypes of pituitary adenomas do not appear to have a predictive role in estimating remission outcomes. Cavernous sinus invasion followed by one-day postoperative GH is the strongest parameter to predict biochemical remission.

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Has-miR-875-5p Inhibits Tumorigenesis by Targeting USF2 via TGF-β Signaling Pathway in Gastric Cancer

10.21203/rs.3.rs-885737/v1 ◽

2021 ◽

Author(s):

Shenshuo Gao ◽

Zhikai Zhang ◽

Xubin Wang ◽

Yan Ma ◽

Chensheng Li ◽

...

Keyword(s):

Gastric Cancer ◽

Signaling Pathway ◽

Research Direction ◽

Luciferase Reporter ◽

Migration And Invasion ◽

Gastric Cancer Cells ◽

New Research

Abstract Background: Gastric cancer (GC) is one of the most common malignancies, and more and more evdiences show that the pathogenesis is regulated by various miRNAs.In this study, we investigated the role of miR-875 in GC. Methods:The expression of miR-875-5p was detected in human GC specimens and cell lines by miRNA RT-PCR. The effect of miR-875-5p on GC proliferation was determined by CCK-8 proliferation assay and EDU assay. Migration and invasion were examined by transwell migration and invasion assay and wound healing assay. The interaction between miR-875-5p and its target gene USF2 was verified by a dual luciferase reporter assay. The effects of miR-875-5p in vivo were studied in xenograft nude mice models.Related proteins were detected by Western blot.Results:The results showed that miR-875-5p inhibited the proliferation, migration and invasion of gastric cancer cells in vitro, and inhibited tumorigenesis in vivo. USF2 proved to be a direct target of miR-875-5p. Knockdown of USF2 partially counteracts the effects of miR-875-5p inhibitors.Overexpression of miR-875-5p can inhibit proliferation, migration, and invasion through the TGF-β signaling pathway by down-regulation of USF2 in GC, providing a new research direction for the diagnosis and targeted therapy of GC.Conclusions: MiR-875-5pcan inhibited the progression of GC by directly targeting USF2 and negatively regulating TGF-β signaling pathway.In the future, miR-875-5p is expected to be used as a potential therapeutic target for GC therapy.

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