The Incidence and Genetic Diversity of Potato virus S in Serbian Seed Potato Crops

2018 ◽  
Vol 62 (1) ◽  
pp. 31-46
Author(s):  
Danijela Ristić ◽  
Ivan Vučurović ◽  
Slobodan Kuzmanović ◽  
Erika Pfaf-Dolovac ◽  
Goran Aleksić ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Seed Potato ◽  
Potato Virus ◽  
Potato Virus S ◽  
Potato Crops

Spatiotemporal Spread of Potato virus S and Potato virus X in Seed Potato in Tasmania, Australia

2007 ◽  
Vol 8 (1) ◽  
pp. 70 ◽  
Author(s):  
Susan J. Lambert ◽  
Frank S. Hay ◽  
Sarah J. Pethybridge ◽  
Calum R. Wilson
Keyword(s):  
Seed Potato ◽  
Potato Virus ◽  
Temporal Distribution ◽  
Potato Virus X ◽  
Russet Burbank ◽  
Mechanical Transmission ◽  
Spatial And Temporal Distribution ◽  
Plant Spacing ◽  
Potato Virus S ◽  
The Mean

The spatial and temporal distribution of Potato virus S (PVS) and Potato virus X (PVX) was studied in two trials within each of four commercial fields of seed potato var. Russet Burbank in Tasmania, Australia. In the first trial (plots) 20 leaflets were collected from each of 49 plots (each approximately 8 m wide by 10 m long), with plots arranged in a 7-×-7 lattice. In the second trial (transects), leaflets were collected at 1-m intervals along seven adjacent, 50-m long rows. The mean incidence of PVS increased during the season by 5.2% in one of four plot trials and 25.5% in one of four transect trials. The mean incidence of PVX increased during the season by 10.1%, in one of two transect trials. Spatial Analysis by Distance IndicEs and ordinary runs analysis detected aggregation of PVS infected plants early in the season in one and two fields respectively, suggesting transmission during seed-cutting or during planting. An increase in PVS incidence mid- to late season in one field was associated with aggregation of PVS along, but not across rows, which may be related to the closer plant spacing within rows and hence increased potential for mechanical transmission along rows. Results suggested limited spread of PVS and PVX occurred within crops during the season. Accepted for publication 9 April 2007. Published 26 July 2007.

Comparison of Straw Mulch, Insecticides, Mineral Oil, and Birch Extract for Control of Transmission of Potato virus Y in Seed Potato Crops

2014 ◽  
Vol 57 (1) ◽  
pp. 59-75 ◽  
Author(s):  
S. M. Kirchner ◽  
L. H. Hiltunen ◽  
J. Santala ◽  
T. F. Döring ◽  
J. Ketola ◽  
...  
Keyword(s):  
Seed Potato ◽  
Potato Virus ◽  
Potato Virus Y ◽  
Mineral Oil ◽  
Straw Mulch ◽  
Potato Crops

scholarly journals One-step real-time multiplex reverse transcription-polymerase chain reaction assay with melt curve analysis for detection of potato leafroll virus, potato virus S, potato virus X, and potato virus Y

2021 ◽  
Vol 18 (1) ◽  
Author(s):  
Nobuya Onozuka ◽  
Takehiro Ohki ◽  
Norikuni Oka ◽  
Tetsuo Maoka
Keyword(s):  
Real Time ◽  
Seed Potato ◽  
Potato Virus ◽  
Potato Virus X ◽  
Virus Species ◽  
Potato Virus S ◽  
Melt Curve Analysis ◽  
One Step ◽  
High Throughput Detection ◽  
Leafroll Virus

Abstract Background Certification of seed potato as free of viruses is essential for stable potato production. Among more than 30 virus species infecting potato, potato leafroll virus (PLRV), potato virus S (PVS), potato virus X (PVX), and potato virus Y (PVY) predominate worldwide and should be the targets of a high-throughput detection protocol for seed potato quarantine. Results We developed an assay based on one-step real-time multiplex reverse transcription-polymerase chain reaction (mRT-PCR) with melt curve analysis for the four viruses and one internal control, potato elongation factor 1 alpha gene (EF1α). Virus-specific primers were derived from conserved regions among randomly selected representatives considering viral genomic diversity. Our assay simultaneously detected representative Japanese isolates of PLRV, O lineage of PVS, PVX, and NTN strain of PVY. The variability of melting temperature (Tm) values for each virus was confirmed using Japanese isolates, and virus species could be identified by the values of 87.6 for PLRV, 85.9 for PVX, 82.2 (Ordinary lineage) to 83.1 (Andean lineage) for PVS, and 79.4 (NA-N strain) to 80.5 (O strain and NTN strain) for PVY on average. The reliability of calculation was validated by comparing the calculated Tm values and measured Tm values and the values had a strong linear correlation (correlation of determination: R2 = 0.9875). Based on the calculated Tm values, representative non-Japanese isolates could also be identified by our assay. For removing false positives, two criteria were set for the evaluation of result; successful amplification was considered as 30.0 ≥ threshold cycle value, and the virus-specific peak higher than the EF1α-specific peak was considered as positive. According to these criteria, our assay could detect PLRV and PVS from 100-fold dilution of potato leaf homogenate and PVX and PVY from 1000-fold in a model assay. Conclusion This new high-throughput detection protocol using one-step real-time mRT-PCR was sensitive enough to detect viruses in a 100-fold dilution of singly-virus contaminated homogenate in a model assay. This protocol can detect the four viruses in one assay and yield faster results for a vast number of samples, and greatly save the labor for seed potato quarantine and field surveys.

scholarly journals Incidence of Potato Viruses in Different Districts of Khyber Pakhtunkhawa, Pakistan

2013 ◽  
Vol 2 (1) ◽  
pp. 32-36 ◽  
Author(s):  
Zishan Gul ◽  
Aftab A. Khan ◽  
Asif U. R. Khan ◽  
Zaheer U. Khan
Keyword(s):  
Potato Virus ◽  
Leaf Roll ◽  
Potato Virus X ◽  
Potato Leaf ◽  
Potato Leave ◽  
Potato Virus S ◽  
Immunosorbant Assay ◽  
Potato Virus A ◽  
Potato Virus M ◽  
Potato Crops

Three consecutive potato crops are grown annually in Pakistan and Khyber Pakhtunkhawa (KPK) province is the main source for seed tubers. During the year 2010, 240 symptomatic and viral susceptible potato leave samples were collected from Swat, Dir, Abbottabad and Mansehra and serologically confirmed through Double Antibody Sandwich (DAS) Enzyme Linked Immunosorbant Assay (ELISA) against Potato virus X (PVX), Potato virus Y (PVY), Potato leaf roll virus (PLRV), Potato virus M (PVM), Potato virus S (PVS) and Potato virus A (PVA). The presence or absence of viruses was confirmed by observing the yellow and white color in ELISA plates respectively. According to ELISA results, overall percentage incidence of viral diseases was found highest in Swat (65%) followed by Mansehra (61%), Dir (53%) and Abbottabad (43.33%) while PVY and PVS were dominant in Swat (26.66%), Dir (20%), Mansehra (15%) and Abbottabad (21.66%) respectively. PVX was not detected in Abbottabad while Swat samples seemed to be free from PVM and PVA.

scholarly journals In silico Characterization and Homology Modelling of Potato Leaf Roll Virus (PLRV) Coat Protein

2019 ◽  
pp. 1-8
Author(s):  
Jitesh Kumar ◽  
Tushar Ranjan ◽  
Ravi Ranjan Kumar ◽  
Mohammad Ansar ◽  
Kumari Rajani ◽  
...  
Keyword(s):  
Coat Protein ◽  
Potato Virus ◽  
Homology Modelling ◽  
Leaf Roll ◽  
Potato Leaf Roll Virus ◽  
Z Score ◽  
Ramachandran Plot ◽  
Potato Leaf ◽  
Potato Virus S ◽  
Potato Crops

Polerovirus (Family-Luteoviridae) are one of the most destructive viruses causing detrimental diseases in vegetable crops in tropical regions of the world including India. Four species viz. potato leaf roll virus (PLRV), potato virus Y(PVY), potato virus X(PVX) and potato virus S(PVS) are known to cause different diseases in potato crops. Of the various viral diseases inflicting potato crops, potato leaf roll disease is the most destructive and widely distributed. They cause huge agro-economical losses (90%) worldwide and thus are the subjects of immense concern. PLRV is a phloem-limited spherical virus transmitted by several aphid species in a persistent manner. A study was performed in order to detect the infection of potato leaf roll virus from different regions of Bihar. These infected samples were diagnosed first using DAS-ELISA for the PLRV infection and later, coat protein was amplified and sequenced from PLRV positive sample. Phylogenetic tree deduced based on the nucleotide sequence of the coat protein gene gene showed a distinct divergence of PLRV isolates in two major clades. The molecular weight of the predicted protein sequence of 203 amino acids was found 22617.06 daltons while theoretical pI was 5.22. The extinction coefficient of predicted coat protein was 0.836. An attempt was taken in order to illustrate the 3D model of the coat protein which was further verified using Ramachandran plot. The model structure obtained using Swiss-Model had 92.9% residues in the most favourable region of the Ramachandran plot (Fig. 3c) and showed Z-score for bond angles, chi-1/chi-2 correlation and Ramachandran Z-score were 1.457, 1.773 and -2.633 respectively which exhibited  considerably good model quality.

scholarly journals Tomato severe rugose virus: Another Begomovirus Causing Leaf Deformation and Mosaic Symptoms on Potato in Brazil

Plant Disease ◽  
2008 ◽  
Vol 92 (3) ◽  
pp. 487-487 ◽  
Author(s):  
J. A. C. Souza-Dias ◽  
H. E. Sawazaki ◽  
P. C. A. Pernambuco-Fo ◽  
L. M. Elias ◽  
H. Maluf
Keyword(s):  
Solanum Tuberosum ◽  
Seed Potato ◽  
Potato Virus ◽  
Mechanical Transmission ◽  
Streak Virus ◽  
Potato Leaf ◽  
Potato Virus S ◽  
Potato Plants ◽  
Datura Metel ◽  
Test Plants

Over the past 10 years, Tomato yellow vein streak virus (ToYVSV) has been a major begomovirus in the main solanaceous crop region of Campinas, São Paulo, which includes counties of Sumaré, Monte Mor, Elias Fausto, and Indaiatuba. The top leaves of potato plants (Solanum tuberosum) having deforming mosaic symptoms (dms), which includes a yellow mosaic or mottling on distorted and deformed leaflets, were associated with this geminivirus (4). Recently, a table potato crop (cv. Agata) from Sumaré, with a record of a few or no white flies (Bemisia tabaci), during the winter season of June to September 2006 had 5 to 7% dms, suggestive of seed potato tuber borne virus infection. Double-antibody sandwich (DAS)-ELISA for Potato virus Y (PVY), Potato leaf roll virus (PLRV), Potato virus X (PVX), and Potato virus S (PVS) (SASA kits and protocols, Edinburgh, Scotland) gave negative results for four field collected potato plants showing dms. Bioassays (mechanical transmission from potato leaf extracts in phosphate buffered saline, 1:5 w/v) with test plants of Nicotiana tabaccum cvs. Turkish and TNN, Gomphrena globosa, Chenopodium quinoa, Datura metel, Solanum tuberosum, and a Physalis sp. were all negative. Inoculated D. stramonium developed symptoms resembling ToYVSV infection including vein clearing and mild mottling on new leaves 2 to 3 weeks postinoculation. Using primers PAC1v1978/PAV1c715 for begomovirus detection (3), the predicted PCR amplified fragment of 1,320 bp was obtained from leaf DNA extracted from all four of the dms field potato plants, as well as the inoculated and symptomatic D. stramoniium test plants. Sequence analysis indicated 100% nt identity among the 1.3-kb PCR fragments obtained from potato and D. stramonium infected plants. Sequences of 96 cloned amplicons (pGEM-T Easy Kit; Promega, Madison, WI) from symptomatic plants in the Sumaré potato field were 98 to 99% identical to Tomato severe rugose virus (ToSRV). BLAST analysis of a consensus sequence (Sequencher 3.1; Gene Codes Corporation, Ann Arbor, MI) revealed more than 95 and 99% identity with ToSRV isolates from Uberlandia (Accession No. AY029750) and Goias (Accession No. DQ207749), respectively. The DNA-based phylogenetic dendrogram confirmed the highest similarity with ToSRV and the lowest similarity with ToYVSV (72%), which was located in another cluster. These results indicate that ToSRV was the causal agent producing dms in potato plants from Sumaré. Therefore, similarly to ToYVSV (4), potato dms can be caused by ToSRV. Preliminary tests revealed that ToSRV was transmitted via seed tubers. Thus, it is of concern for seed potato certification in Brazil, especially in the major seed-potato-producing state of Santa Catarina where an outbreak of ToSRV was recently reported in tomato crops (1). Although ToSRV has been identified in other solanaceous crops in Brazil, especially tomato (Lycopersicon esculentum) and sweet pepper (Capsicum annum) (2), to our knowledge, this is the first report of ToSRV in potato in Brazil. Reference: (1) A. T. M. Lima et al. Fitopatol. Bras. 31:224, 2006. (2) D. N. Nozaki et al. Summa Phytopathol. 33:93, 2007. (3) M. R. Rojas et al. Plant Dis. 77:340, 1993. (4) J. A. C. Souza-Dias et al. CultivarHF 5(26):22, 2004.

scholarly journals Genome characterization of a Potato virus S (PVS) variant from tuber sprouts of Solanum phureja Juz. et Buk

2016 ◽  
Vol 34 (1) ◽  
pp. 51-60 ◽  
Author(s):  
Daniela Vallejo C. ◽  
Pablo Andrés Gutiérrez S. ◽  
Mauricio Marín M.
Keyword(s):  
Potato Virus ◽  
High Throughput Sequencing ◽  
Solanum Phureja ◽  
High Sequence Identity ◽  
Illumina Hiseq ◽  
Potato Virus S ◽  
The World ◽  
Prevalent Virus ◽  
Potato Crops ◽  
Genome Information

Potato virus S (PVS) is a prevalent virus in potato fields in Colombia and the rest of the world. PVS has been classified into two separate lineages, PVSO (Ordinary) and PVSA (An- dean), which are genetically distinct. In this study, the com- plete genome sequence of a new PVS isolate (PVS_Antioquia) was obtained using High-throughput sequencing (Illumina HiSeq-2000) from tuber sprouts of Solanum phureja (var. Criolla Colombia). The PVS_Antioquia genome comprises 8,483 nt that code for six ORFs: RdRp (223 kDa), TGBp1-3 (25kDa, 12kDa, 7kDa) CP (32.3 kDa) and NABP (11 kDa) and share a high sequence identity with respect to the PVS_RVC (>95%) from Colombia, in contrast to 81 to 82% identity with respect to the PVSA and PVSO isolates from around the world. This genome information was used to design RT-qPCR primers that are specific for the Colombian PVS strains (RVC and Antioquia) which were validated in S. phureja leaf and tuber samples. These primers detected PVS in 80 and 60% of a set of fifteen leaf and tuber samples, respectively, suggesting a high incidence of this virus in the potato crops of Antioquia.

scholarly journals Distribution and incidence of viruses in Irish seed potato crops

2015 ◽  
Vol 54 (2) ◽  
pp. 98-106 ◽  
Author(s):  
F. Hutton ◽  
J.H. Spink ◽  
D. Griffin ◽  
S. Kildea ◽  
D. Bonner ◽  
...  
Keyword(s):  
Potato Virus ◽  
Significant Proportion ◽  
Potato Production ◽  
Weather Data ◽  
Management Approach ◽  
Potato Seed ◽  
Virus Diseases ◽  
Aphid Control ◽  
Daily Weather Data ◽  
Potato Crops

Abstract Virus diseases are of key importance in potato production and in particular for the production of disease-free potato seed. However, there is little known about the frequency and distribution of potato virus diseases in Ireland. Despite a large number of samples being tested each year, the data has never been collated either within or across years. Information from all known potato virus testing carried out in the years 2006–2012 by the Department of Agriculture Food and Marine was collated to give an indication of the distribution and incidence of potato virus in Ireland. It was found that there was significant variation between regions, varieties, years and seed classes. A definition of daily weather data suitable for aphid flight was developed, which accounted for a significant proportion of the variation in virus incidence between years. This use of weather data to predict virus risk could be developed to form the basis of an integrated pest management approach for aphid control in Irish potato crops.

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