Altered gene expression in lymphoblastoid cell lines after subculture

2018 ◽  
Vol 54 (7) ◽  
pp. 523-527 ◽  
Author(s):  
Michihiro Toritsuka ◽  
Manabu Makinodan ◽  
Takahira Yamauchi ◽  
Yasunori Yamashita ◽  
Daisuke Ikawa ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Lines ◽  
Lymphoblastoid Cell Lines ◽  
Altered Gene Expression ◽  
Altered Gene

scholarly journals Modeling human prostate cancer progression in vitro

2018 ◽  
Vol 40 (7) ◽  
pp. 893-902 ◽  
Author(s):  
Teresa T Liu ◽  
Jonathan A Ewald ◽  
Emily A Ricke ◽  
Robert Bell ◽  
Colin Collins ◽  
...  
Keyword(s):  
Gene Expression ◽  
Prostate Cancer ◽  
Cell Lines ◽  
Cancer Progression ◽  
Human Prostate ◽  
Molecular Characteristics ◽  
Prostate Cancer Progression ◽  
Altered Gene Expression ◽  
Unique Model ◽  
Altered Gene

Abstract Detailed mechanisms involved in prostate cancer (CaP) development and progression are not well understood. Current experimental models used to study CaP are not well suited to address this issue. Previously, we have described the hormonal progression of non-tumorigenic human prostate epithelial cells (BPH1) into malignant cells via tissue recombination. Here, we describe a method to derive human cell lines from distinct stages of CaP that parallel cellular, genetic and epigenetic changes found in patients with cancers. This BPH1-derived Cancer Progression (BCaP) model represents different stages of cancer. Using diverse analytical strategies, we show that the BCaP model reproduces molecular characteristics of CaP in human patients. Furthermore, we demonstrate that BCaP cells have altered gene expression of shared pathways with human and transgenic mouse CaP data, as well as, increasing genomic instability with TMPRSS2–ERG fusion in advanced tumor cells. Together, these cell lines represent a unique model of human CaP progression providing a novel tool that will allow the discovery and experimental validation of mechanisms regulating human CaP development and progression. This BPH1-derived Cancer Progression (BCaP) model represents different stages of cancer. The BCaP model reproduces molecular characteristics of prostate cancer. The cells have altered gene expression with TMPRSS2-ERG fusion representing a unique model for prostate cancer progression.

Epigenetic drug combination induces genome-wide demethylation and altered gene expression in neuro-ectodermal tumor-derived cell lines

2013 ◽  
Vol 36 (5) ◽  
pp. 351-362 ◽  
Author(s):  
Floor A.M. Duijkers ◽  
Renee X. de Menezes ◽  
Inès J. Goossens-Beumer ◽  
Dominique J.P.M. Stumpel ◽  
Pieter Admiraal ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Lines ◽  
Drug Combination ◽  
Altered Gene Expression ◽  
Genome Wide ◽  
Epigenetic Drug ◽  
Altered Gene

Abstract A19: Epigenetic drug combination induces genome-wide demethylation and altered gene expression in neuro-ectodermal tumor-derived cell lines

2014 ◽  
Author(s):  
Max M. van Noesel ◽  
Floor A M Duijkers ◽  
Renee X. de Menezes ◽  
Dominique J. P. M. Stumpel ◽  
Pieter Admiraal ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Lines ◽  
Drug Combination ◽  
Altered Gene Expression ◽  
Genome Wide ◽  
Epigenetic Drug ◽  
Altered Gene

Molecular and Microscopic Analysis of Altered Gene Expression in Transgenic and Gene Targeted Mice

1996 ◽  
Vol 54 ◽  
pp. 12-13
Author(s):  
W. K. Jones ◽  
J. Robbins
Keyword(s):  
Gene Expression ◽  
Pulmonary Veins ◽  
Microscopic Analysis ◽  
Mouse Tissue ◽  
Adult Heart ◽  
Heavy Chains ◽  
Altered Gene Expression ◽  
Altered Gene ◽  
Pulmonary Myocardium

Two myosin heavy chains (MyHC) are expressed in the mammalian heart and are differentially regulated during development. In the mouse, the α-MyHC is expressed constitutively in the atrium. At birth, the β-MyHC is downregulated and replaced by the α-MyHC, which is the sole cardiac MyHC isoform in the adult heart. We have employed transgenic and gene-targeting methodologies to study the regulation of cardiac MyHC gene expression and the functional and developmental consequences of altered α-MyHC expression in the mouse.We previously characterized an α-MyHC promoter capable of driving tissue-specific and developmentally correct expression of a CAT (chloramphenicol acetyltransferase) marker in the mouse. Tissue surveys detected a small amount of CAT activity in the lung (Fig. 1a). The results of in situ hybridization analyses indicated that the pattern of CAT transcript in the adult heart (Fig. 1b, top panel) is the same as that of α-MyHC (Fig. 1b, lower panel). The α-MyHC gene is expressed in a layer of cardiac muscle (pulmonary myocardium) associated with the pulmonary veins (Fig. 1c). These studies extend our understanding of α-MyHC expression and delimit a third cardiac compartment.

Polymorphism of the MTHFR Gene is Associated with Altered Gene Expression in Colorectal Cancer

Endoscopy ◽  
2004 ◽  
Vol 36 (05) ◽  
Author(s):  
K Collins ◽  
GA Doherty ◽  
MR Sweeney ◽  
SM Byrne ◽  
AA Aftab ◽  
...  
Keyword(s):  
Gene Expression ◽  
Colorectal Cancer ◽  
Mthfr Gene ◽  
Altered Gene Expression ◽  
Altered Gene
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