Behavior of a cell line derived from normal human hepatocytes on non-physiological and physiological-type substrates: Evidence for enhancement of secretion of liver-specific proteins by a three-dimensional growth pattern

1999 ◽  
Vol 35 (1) ◽  
pp. 22-32 ◽  
Author(s):  
Matthew Smalley ◽  
Kenneth Leiper ◽  
David Floyd ◽  
Margaret Mobberley ◽  
Tim Ryder ◽  
...  
Keyword(s):  
Cell Line ◽  
Growth Pattern ◽  
Three Dimensional ◽  
Human Hepatocytes ◽  
A Cell ◽  
Specific Proteins ◽  
Normal Human ◽  
Dimensional Growth ◽  
Physiological Type

scholarly journals OCCURRENCE OF FOCAL THREE-DIMENSIONAL PROLIFERATION IN CULTURED HUMAN CELLS AFTER PROLONGED INFECTION WITH HERPES SIMPLEX VIRUS

1961 ◽  
Vol 113 (5) ◽  
pp. 885-898 ◽  
Author(s):  
Harry C. Hinze ◽  
Duard L. Walker
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Human Serum ◽  
Cell Line ◽  
Growth Pattern ◽  
Herpes Virus ◽  
Three Dimensional ◽  
Multiplication Rate ◽  
Herpès Virus ◽  
Simplex Virus

The occurrence of focal three-dimensional proliferation was observed in human conjunctiva cells in culture after prolonged infection with herpes simplex virus at 30°C. and in KB cells infected with herpes virus at 37°C. for several months in the presence of pooled human serum. This atypical growth pattern consisted of many discrete piles of cells 15 to 20 cells thick scattered over a monolayer of cells. Three-dimensional growth appeared only in the presence of 10 to 20 per cent of selected human sera. When horse serum was substituted for human serum growth was in an ordinary monolayer. The abnormal growth pattern of this cell line (C-4 line) has remained stable through continuous culture for over 2 years and was observed consistently in each of 25 clones isolated from the parent culture. Repeated tests for the presence of persisting infectious virus and for herpes virus antigen in the cells were negative. Comparison of the C-4 cell line with control conjunctiva cells revealed no significant difference in multiplication rate or in range of viral susceptibility, but the C-4 line was found to be more sensitive to ultraviolet irradiation.

Modulation of different stress pathways after styrene and styrene-7,8-oxide exposure in HepG2 cell line and normal human hepatocytes

2006 ◽  
Vol 26 (4) ◽  
pp. 317-325 ◽  
Author(s):  
Cristina Diodovich ◽  
Chiara Urani ◽  
Daniela Maurici ◽  
Ilaria Malerba ◽  
Pasquale Melchioretto ◽  
...  
Keyword(s):  
Cell Line ◽  
Hepg2 Cell ◽  
Human Hepatocytes ◽  
Hepg2 Cell Line ◽  
Normal Human

Three-dimensional growth pattern of the rat mandible revealed by periodic live micro-computed tomography

2018 ◽  
Vol 87 ◽  
pp. 94-101 ◽  
Author(s):  
Hak-Jin Kim ◽  
Kyeong-Mee Park ◽  
Hye-Jin Tak ◽  
Ji Wook Choi ◽  
Sang-Hoon Kang ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Growth Pattern ◽  
Three Dimensional ◽  
Micro Computed Tomography ◽  
Dimensional Growth ◽  
Rat Mandible

Aberrant Type C Virus Production in a Cell Line Derived from Balb/3T3

1972 ◽  
Vol 30 ◽  
pp. 286-287
Author(s):  
N. Savage ◽  
A. Hackett
Keyword(s):  
Electron Microscopy ◽  
Cell Line ◽  
Leukemia Virus ◽  
Morphological Characteristics ◽  
Virus Production ◽  
Oncogenic Viruses ◽  
Endogenous Virus ◽  
Virus Particles ◽  
Moloney Leukemia Virus ◽  
A Cell

A cell line, UC1-B, which was derived from Balb/3T3 cells, maintains the same morphological characteristics of the non-transformed parental culture, and shows no evidence of spontaneous virus production. Survey by electron microscopy shows that the cell line consists of spindle-shaped cells with no unusual features and no endogenous virus particles.UC1-B cells respond to Moloney leukemia virus (MLV) infection by a change in morphology and growth pattern which is typical of cells transformed by sarcoma virus. Electron microscopy shows that the cells are now variable in shape (rounded, rhomboid, and spindle), and each cell type has some microvilli. Virtually all (90%) of the cells show virus particles developing at the cell surface and within the cytoplasm. Maturing viruses, typical of the oncogenic viruses, are found along with atypical tubular forms in the same cell.

Hormonal modulation of ishikawa cells during three-dimensional growth in vitro☆

1998 ◽  
Vol 5 (4) ◽  
pp. 217-223 ◽  
Author(s):  
D PINELLI ◽  
J DRAKE ◽  
M WILLIAMS ◽  
D CAVANAGH ◽  
J BECKER
Keyword(s):  
Three Dimensional ◽  
Ishikawa Cells ◽  
Hormonal Modulation ◽  
Dimensional Growth
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