Responses of Arabica coffee (Coffea arabica L. var. Catuaí) cell suspensions to chemically induced mutagenesis and salinity stress under in vitro culture conditions

2018 ◽  
Vol 54 (6) ◽  
pp. 576-589 ◽  
Author(s):  
Alejandro Bolívar-González ◽  
Marta Valdez-Melara ◽  
Andrés Gatica-Arias
Keyword(s):  
In Vitro Culture ◽  
Salinity Stress ◽  
Coffea Arabica ◽  
Culture Conditions ◽  
Cell Suspensions ◽  
Arabica Coffee ◽  
Chemically Induced ◽  
Induced Mutagenesis ◽  
Coffea Arabica L

scholarly journals Effect of Salinity on Morphological Characteristics, Biochemical Accumulation, and Essential Oil Yield and Composition in Black Horehound (Ballota nigra L.)

2021 ◽  
Author(s):  
Mehdi Younessi Mehdikhanlou ◽  
Zahra Dibazarnia ◽  
Shahin Oustan ◽  
Vinson Teniyah ◽  
Ramesh Katam ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Essential Oil ◽  
In Vitro Culture ◽  
Salinity Stress ◽  
Salt Concentration ◽  
Total Phenolics ◽  
Morphological Characteristics ◽  
Culture Conditions ◽  
Total Flavonoids

Black horehound (Ballota nigra L.) is one of the important medicinal plants, which is a rich source of health-promoting essential oils. Salinity stress affects plant development and alters the quality and quantity of plants extracts and their composition. This study was aimed to investigate the effect of salinity on morphological, physiological characteristics, and secondary metabolites of B. nigra under greenhouse, and in vitro culture conditions. The plants were treated with different concentrations of NaCl (25, 50, 75, 100 mM) and fresh and dry weight of leaf and stem were measured as well as morphological characteristics of the plant. Plant growth was reduced with the increased salinity concentrations. The results showed that all growth-related traits and SPAD were decreased both in vivo and in vitro. Additionally, increased salt concentration affected the cell membrane integrity. Total phenolics content of plants growing in the greenhouse, increased by 21% at 50 mM NaCl, but at higher stress levels (100 mM NaCl), the amounts were decreased significantly. Total flavonoids contents followed similar patterns, with a slight difference. In addition, the maximum and minimum total phenolics contents of plants growing under in vitro condition were observed at 50 mM NaCl and control treatments, respectively. Increasing the salt concentration significantly affected the total flavonoids content, and as a result, the highest amount was observed in 50 and 75 mM NaCl treatments. Antioxidant activity was also measured. Among the NaCl treatments, the highest DPPH scavenging activities (IC50) under greenhouse and in vitro conditions were detected at 50 mM and 25 mM concentrations, respectively. In general, based on the results, with increasing the salinity level to 75 mM, the activities of CAT and APX were significantly upregulated in both greenhouse and in vitro culture conditions. A correlation between total phenolics and flavonoids contents as well as antioxidant activity were obtained. With shifting salinity stress, the type and the amount of the identified essential oil compounds changed. Compounds such as styrene, tridecanol, germacrene-D, beta-Ionone, beta-bisabolene, and caryophyllene oxide increased compared to the controlled treatment.

Responses of grass pea seedlings to salinity stress in in vitro culture conditions

2015 ◽  
Vol 124 (2) ◽  
pp. 227-240 ◽  
Author(s):  
Barbara Piwowarczyk ◽  
Krzysztof Tokarz ◽  
Iwona Kamińska
Keyword(s):  
In Vitro Culture ◽  
Salinity Stress ◽  
Culture Conditions ◽  
Grass Pea ◽  
Pea Seedlings

scholarly journals Mild Salinity Stimulates Biochemical Activities and Metabolites Associated with Anticancer Activities in Black Horehound (Ballota nigra L.)

Agronomy ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 2538
Author(s):  
Mehdi Younessi-Hamzekhanlu ◽  
Zahra Dibazarnia ◽  
Shahin Oustan ◽  
Teniyah Vinson ◽  
Ramesh Katam ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Salt Stress ◽  
In Vitro Culture ◽  
Salinity Stress ◽  
Membrane Integrity ◽  
Morphological Characteristics ◽  
Culture Conditions ◽  
Salinity Level ◽  
Total Phenolic

Black horehound (Ballota nigra L.) is one of the most important medicinal plants, as a rich source of health-promoting essential oils and metabolites. Salinity stress affects plant development and alters antioxidant activity and plant metabolite composition. The present research aimed to study the effect of salinity on physiological and biochemical changes and metabolites of B. nigra under greenhouse and in vitro culture conditions. The plants were treated with different concentrations of NaCl (25, 50, 75, 100 mM), and morphological characteristics of the plant were measured. The growth-related traits and soil plant analysis development (SPAD) were decreased both in vivo and in vitro. Additionally, increased salt concentration negatively affected the cell membrane integrity. The total phenolic content and flavonoids of plants growing in the greenhouse increased by 21% at 50 mM of NaCl, but the amounts decreased significantly at higher stress levels (100 mM of NaCl). Antioxidant activity was also measured. Among the NaCl treatments, the most increased DPPH scavenging activities (IC50) under greenhouse and in vitro conditions were detected at mild salinity stress, but the activity significantly decreased in higher salinity treatments (i.e., 75 and 100 mM). In general, with increasing the salinity level to 75 mM, the activities of CAT and APX were significantly upregulated in both greenhouse and in vitro culture conditions. A correlation between total phenolics and flavonoids contents as well as antioxidant activity was obtained. Salinity level caused a shift in the metabolite expression. Mild salinity stress elevated the metabolites involved in anticancer and anti-inflammatory activities, such as β-ionone and caryophyllene oxide. However, the higher salt stress resulted in a significant reduction in their expression. Differential expression of metabolites to various levels of salt stress can be further exploited for the in vitro biosynthesis of metabolites.

scholarly journals Evaluation of in Vitro Culture Conditions to Demonstrate Pregnancy-Dependent Changes in Luteal Function in the Pig1

1994 ◽  
Vol 51 (2) ◽  
pp. 254-261 ◽  
Author(s):  
Teresa Wiesak ◽  
Robert T. Hardin ◽  
George R. Foxcroft
Keyword(s):  
In Vitro Culture ◽  
Culture Conditions ◽  
Luteal Function

Ploidy instability in long-term in vitro cultures of Coffea arabica L. monitored by flow cytometry

2012 ◽  
Vol 68 (3) ◽  
pp. 533-538 ◽  
Author(s):  
Wellington Ronildo Clarindo ◽  
Carlos Roberto Carvalho ◽  
Maria Andréia Corrêa Mendonça
Keyword(s):  
Flow Cytometry ◽  
Coffea Arabica ◽  
In Vitro Cultures ◽  
Coffea Arabica L

scholarly journals The effect of auxin 2,4-D and cytokinin 2-ip on direct somatic embryogenesis formation of Coffea arabica L. leaf explant

2011 ◽  
Vol 27 (2) ◽  
Author(s):  
Rina Arimarsetiowati
Keyword(s):  
Tissue Culture ◽  
Somatic Embryogenesis ◽  
Coffea Arabica ◽  
Callus Formation ◽  
Culture Technique ◽  
Plant Production ◽  
Direct Somatic Embryogenesis ◽  
Arabica Coffee ◽  
Randomized Design ◽  
Coffea Arabica L

One of the propagation technique for coffee plant production is tissue culture. Tissue culture technique for Coffea arabica L. faces some problems, mainly in the planlet formation regenerated from explants. The objective of this experiment was to examine the effect 2,4-D and 2-ip combination on the formation of direct somatic embryogenesis of Coffea arabica L. in leaves explant. Auxin (2,4-D) and cytokinin (2-ip) concentrations of, respectively, 1; 5 µM and 5; 10; 15; 20 were used as treatments. This research was conducted using completely randomized design with 10 replications. Observation to induce somatic embryos was done by quantitatively on number of callus from explant and number of embryogenic callus. Beside that, observation by qualitative descriptive was also done on deve lopment of embryogenesis. The results showed that Arabica coffee leaves explant of AS 2K clones could be induced in all medium combination except 5µM 2,4-D and 20µM 2-ip combination. Arabica coffee leaves explant of S 795, Sigararutang and AS 1 varieties could be induced in all medium combination. The highest frequency of callus formation was found in AS 2K, Sigararutang and AS 1 varieties on medium containing 1µM 2,4-D in combination with 10µM 2-ip, whereas for the S 795 variety on medium containing 5µM 2,4-D in combination with 10µM 2-ip. The highest frequency of embriogenic callus in all Arabica coffee variety could be reached on medium containing 5µM 2,4-D in combination with 15µM 2-ip. Key words : Coffea arabica L., somatic embryogenesis, 2,4-D, 2-ip, tissue culture, leaves, callus embryogenic.

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