Correction to: Effects of chemical sterilization of the culture media, porous membranes and luminosity on in vitro culture of Eucalyptus grandis × Eucalyptus urophylla

In vitro culture is an important biotechnological tool in plant research and an appropriate culture media is a key for a successful plant development under in vitro conditions. The use of natural compounds to improve culture media has been growing and biopolymers are interesting alternatives to synthetic compounds due to their low toxicity, biodegradability, renewability, and availability. In the present study, different culture media containing one biopolymer (chitosan, gum arabic) or a biopolymer derivative [hydroxyethyl cellulose (HEC), carboxymethyl cellulose (CMC)], at 100 or 1000 mg L−1, were tested regarding their influence on the growth and physiological responses of Thymus lotocephalus in vitro culture. Cellulose-based biopolymers (HEC and CMC) and gum arabic were used for the first time in plant culture media. The results showed that CMC at 100 mg L−1 significantly improved shoot elongation while chitosan, at the highest concentration, was detrimental to T. lotocephalus. Concerning only the evaluated physiological parameters, all tested biopolymers and biopolymer derivatives are safe to plants as there was no evidence of stress-induced changes on T. lotocephalus. The rheological and microstructural features of the culture media were assessed to understand how the biopolymers and biopolymer derivatives added to the culture medium could influence shoot growth. As expected, all media presented a gel-like behaviour with minor differences in the complex viscosity at the beginning of the culture period. Most media showed increased viscosity overtime. The surface area increased with the addition of biopolymers and biopolymer derivatives to the culture media and the average pore size was considerably lower for CMC at 100 mg L−1. The smaller pores of this medium might be related to a more efficient nutrients and water uptake by T. lotocephalus shoots, leading to a significant improvement in shoot elongation. In short, this study demonstrated that the different types of biopolymers and biopolymer derivatives added to culture medium can modify their microstructure and at the right concentrations, are harmless to T. lotocephalus shoots growing in vitro, and that CMC improves shoot length.

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In vitro culture as an aid to conservation of indigenous ferns: Diplazium proliferum

International Journal of Plant Biology ◽

10.4081/pb.2015.6020 ◽

2015 ◽

Vol 6 (1) ◽

Author(s):

Zubeir M. Golamaully ◽

Vishwakalyan Bhoyroo ◽

Nadeem Nazurally ◽

Vineshwar Gopal

Keyword(s):

Growth Rate ◽

In Vitro Culture ◽

Mercuric Chloride ◽

Rare Species ◽

Vascular Plants ◽

Culture Media ◽

Fungal Contamination ◽

Increase Growth Rate ◽

Growing Population

With the ever growing population and economic needs of Mauritius, the flora of Mauritius has never been in more danger and one group of vascular plants is even more in peril; ferns. Diplazium proliferum is indigenous to the Mascarene region and is considered as a rare species in Mauritius. The need to develop a tested in vitro propagation protocol is a must to protect the biodiversity of Mauritius. This experiment was geared towards the establishment of a proper sterilization technique and the effect of 6-benzylaminopurine (BAP) and light on in vitro culture of this fern. Sterilization with 0.05% Mercuric chloride was effective to eliminate fungal contamination and allow germination of spores. Culture media supplemented with BAP did not significantly increase growth rate of both gametophytes and sporophytes of D. proliferum. Present results suggest efficient sterilization methods to be a crucial stage for successful in vitro regeneration of ferns. The established protocol will be used as an optimized baseline protocol for the propagation of other indigenous ferns.

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IBA AND MICROCUTTING COLLECTIONS IN THE MICROPROPAGATION OF Eucalyptus spp HYBRID CLONES.1

Revista Árvore ◽

10.1590/1806-90882017000600005 ◽

2017 ◽

Vol 41 (6) ◽

Author(s):

Ricardo Gallo ◽

Aloisio Xavier ◽

Luciana Coelho de Moura ◽

Brener de Almeida Oliveira ◽

Heloisa Rocha do Nascimento ◽

...

Keyword(s):

Eucalyptus Grandis ◽

Stem Diameter ◽

Residual Effects ◽

Seedling Height ◽

Seedling Vigor ◽

Eucalyptus Urophylla ◽

Ex Vitro ◽

Semisolid Medium ◽

Hybrid Clones

ABSTRACT This study aims to evaluate the effect of IBA concentrations and microcuttings successive collections in the micropropagation of Eucalyptus grandis x E. urophylla and Eucalyptus urophylla x E. globulus clones. Clumps containing six to eight buds of clones established in vitro were transferred to a 250 mL glass flask in JADS semisolid medium. Successive collections were performed every 20 days for Eucalyptus grandis x E. urophylla clone and every 30 days for Eucalyptus urophylla x E. globulus clone. The following variables were evaluated under in vitro conditions: number of shoots > 0.5 cm, number of microcuttings > 2 cm, length of the longest microcutting, and shoots vigor. Under ex vitro conditions, in the greenhouse and shade house, the following variables were evaluated: seedling height, percentage of survival, stem diameter, percentage of root observed at the lower end of the tube, and seedling vigor. In full sun (ex vitro), the following variables were analyzed: seedling height, stem diameter, survival, number of roots, root volume, seedling vigor, and shoot and root dry matter. Good in vitro microcuttings productivity was observed over the successive collections. IBA levels were adjusted for each clone, ranging from 0.25 to 0.50 mg L-1 for Eucalyptus grandis x E. urophylla clone, and from 0.75 to 1.0 mg L-1 for Eucalyptus urophylla x E. globulus clone. IBA concentrations led to residual effects under ex vitro conditions, providing good rooting and survival for Eucalyptus grandis x E. urophylla and Eucalyptus urophylla x E. globulus clones at IBA concentrations between 0.25 and 0.50 mg L-1 and between 0.50 and 1.0 mg L-1, respectively.

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Combining Medicinal Plant In Vitro Culture with Machine Learning Technologies for Maximizing the Production of Phenolic Compounds

Antioxidants ◽

10.3390/antiox9030210 ◽

2020 ◽

Vol 9 (3) ◽

pp. 210 ◽

Cited By ~ 9

Author(s):

Pascual García-Pérez ◽

Eva Lozano-Milo ◽

Mariana Landín ◽

Pedro Pablo Gallego

Keyword(s):

Machine Learning ◽

Phenolic Compounds ◽

In Vitro Culture ◽

Culture Media ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Total Phenolic ◽

Starting Point ◽

Plant In Vitro Culture

We combined machine learning and plant in vitro culture methodologies as a novel approach for unraveling the phytochemical potential of unexploited medicinal plants. In order to induce phenolic compound biosynthesis, the in vitro culture of three different species of Bryophyllum under nutritional stress was established. To optimize phenolic extraction, four solvents with different MeOH proportions were used, and total phenolic content (TPC), flavonoid content (FC) and radical-scavenging activity (RSA) were determined. All results were subjected to data modeling with the application of artificial neural networks to provide insight into the significant factors that influence such multifactorial processes. Our findings suggest that aerial parts accumulate a higher proportion of phenolic compounds and flavonoids in comparison to roots. TPC was increased under ammonium concentrations below 15 mM, and their extraction was maximum when using solvents with intermediate methanol proportions (55–85%). The same behavior was reported for RSA, and, conversely, FC was independent of culture media composition, and their extraction was enhanced using solvents with high methanol proportions (>85%). These findings confer a wide perspective about the relationship between abiotic stress and secondary metabolism and could serve as the starting point for the optimization of bioactive compound production at a biotechnological scale.

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474 Effect of four assisted hatching techniques and two in vitro culture media on embryo hatching rate

Journal of Animal Science ◽

10.2527/asasann.2017.474 ◽

2017 ◽

Vol 95 (suppl_4) ◽

pp. 231-231

Author(s):

N. C. Negota ◽

L. P. Nethenzheni ◽

N. R. Serota

Keyword(s):

In Vitro Culture ◽

Culture Media ◽

Assisted Hatching ◽

Hatching Rate

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160 COMPARATIVE STUDY OF IN VITRO CULTURE MEDIA AND ASSISTED HATCHING TECHNIQUES ON MOUSE EMBRYOS

Reproduction Fertility and Development ◽

10.1071/rdv29n1ab160 ◽

2017 ◽

Vol 29 (1) ◽

pp. 188

Author(s):

N. C. Negota ◽

L. P. Nethenzheni ◽

M. L. Mphaphathi ◽

D. M. Barry ◽

T. L. Nedambale

Keyword(s):

In Vitro Culture ◽

Chorionic Gonadotropin ◽

Culture Medium ◽

Culture Media ◽

Assisted Hatching ◽

Hatching Rate ◽

Significant Difference ◽

Equine Chorionic Gonadotropin ◽

F1 Generation

The in vitro culture media and assisted hatching techniques remain challenging obstacles to be utilised widely. Mechanical, chemical, enzymatic thinning, and laser-assisted techniques have been used previously but information is still lacking on its application in livestock. The aim of this study was to compare the effect of 2 in vitro culture media (Hamster F10 and TMC-199) and 4 (mechanical, chemical, enzymatic, and laser) assisted hatching techniques on blastocyst formation and hatching rate using murine embryos as a model. The C57/b and Balb/c breeds were raised until they reached maturity and bred naturally to produce F1 generation. The light in the breeding house was controlled at 14 h light and 10 h dark. Feed and water were provided ad libitum for the mice. Superovulation of females were stimulated using equine chorionic gonadotropin and human chorionic gonadotropin. The F1 generation was used for the collection of the 400 blastocysts and randomly allocated into 4 assisted hatching techniques. Blastocysts were paired into a group of 10 and replicated 4 times for each assisted hatching technique. The general linear model of SAS version 9.4 (SAS Institute Inc., Cary, NC, USA) was used to analyse the data. Assisted hatching techniques of laser, mechanical, enzymatic, and chemical yielded 46.9 ± 37.1, 51.1 ± 40.2, 39.1 ± 35.8, and 33.3 ± 4.5%, respectively, under in vitro culture of Hamster F10. The TCM-199, laser, mechanical, enzymatic, and chemical assisted hatching techniques yielded 56.3 ± 43.3, 52.6 ± 35.5, 49.2 ± 37.5, and 33.9 ± 35.5%, respectively, with a significant difference. There was no significant difference observed in assisted hatching techniques and Hamster F10 culture medium. However, the hatching rate of embryos for all techniques was higher when in vitro cultured in TCM than cultured in Hamster F10. Hatching rate of blastocysts increased from chemical, enzymatic, mechanical, and laser with response to Hamster F10 and TCM; thus, laser is a suitable assisted hatching technique with TCM-199.

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In vitro culture of pointed gourd (Trichosanthes dioica Roxb.)

Bangladesh Journal of Agricultural Research ◽

10.3329/bjar.v35i1.5874 ◽

1970 ◽

Vol 35 (1) ◽

pp. 135-142 ◽

Cited By ~ 2

Author(s):

MA Malek ◽

D Khanam ◽

M Khatun ◽

MH Molla ◽

MA Mannan

Keyword(s):

Plant Regeneration ◽

In Vitro Culture ◽

Culture Media ◽

Culture Conditions ◽

Root Induction ◽

Ms Medium ◽

Trichosanthes Dioica ◽

Pointed Gourd ◽

Regenerated Plantlets

An experiment was conducted to study the in vitro culture of pointed gourd. Cotyledon rescued from physiologically matured seeds (PMS) and immatured seeds (IMS) of pointed gourd were used as explants. Cotyledon excised from PMS responded very well in all culture conditions. Plant regenerated from cotyledon of PMS ranged from 38 to 96% in different hormonal formulations of culture media. Highest percentage of shoot regeneration was observed in MS + 1.0 mg/l BAP and lowest in MS + 2.5 mg/l BAP. No plant regeneration was observed in cotyledon from immatured seeds. The highest percentage of root induction (99%) was achieved in half MS medium supplemented with 0.5 mg/l NAA. The regenerated plantlets were successfully established in earthen pot. Keywords: Cotyledon; in vitro; pointed gourd. DOI: 10.3329/bjar.v35i1.5874Bangladesh J. Agril. Res. 35(1) : 135-142, March 2010

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