Exploring the effect of acupuncture plus mild hypothermia on miRNA-204 and its target gene expressions in CIRI rat brain tissues based on MAPK signal pathway

2021 ◽  
Vol 19 (5) ◽  
pp. 338-344
Author(s):  
Tang Yu-lan ◽  
Liu Mai-lan ◽  
Luo Jian ◽  
Li Nan ◽  
Zhang Guo-shan ◽  
...  
Keyword(s):  
Rat Brain ◽  
Target Gene ◽  
Mild Hypothermia ◽  
Signal Pathway ◽  
Gene Expressions ◽  
Mapk Signal Pathway ◽  
Brain Tissues

scholarly journals SPAK-p38 MAPK signal pathway modulates claudin-18 and barrier function of alveolar epithelium after hyperoxic exposure

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Chih-Hao Shen ◽  
Jr-Yu Lin ◽  
Cheng-Yo Lu ◽  
Sung-Sen Yang ◽  
Chung-Kan Peng ◽  
...  
Keyword(s):  
P38 Mapk ◽  
Knockout Mice ◽  
Barrier Function ◽  
Alveolar Epithelium ◽  
Signal Pathway ◽  
Control Group ◽  
Barrier Dysfunction ◽  
Hyperoxic Exposure ◽  
Monolayer Permeability ◽  
Mapk Signal Pathway

Abstract Background Hyperoxia downregulates the tight junction (TJ) proteins of the alveolar epithelium and leads to barrier dysfunction. Previous study has showed that STE20/SPS1-related proline/alanine-rich kinase (SPAK) interferes with the intestinal barrier function in mice. The aim of the present study is to explore the association between SPAK and barrier function in the alveolar epithelium after hyperoxic exposure. Methods Hyperoxic acute lung injury (HALI) was induced by exposing mice to > 99% oxygen for 64 h. The mice were randomly allotted into four groups comprising two control groups and two hyperoxic groups with and without SPAK knockout. Mouse alveolar MLE-12 cells were cultured in control and hyperoxic conditions with or without SPAK knockdown. Transepithelial electric resistance and transwell monolayer permeability were measured for each group. In-cell western assay was used to screen the possible mechanism of p-SPAK being induced by hyperoxia. Results Compared with the control group, SPAK knockout mice had a lower protein level in the bronchoalveolar lavage fluid in HALI, which was correlated with a lower extent of TJ disruption according to transmission electron microscopy. Hyperoxia down-regulated claudin-18 in the alveolar epithelium, which was alleviated in SPAK knockout mice. In MLE-12 cells, hyperoxia up-regulated phosphorylated-SPAK by reactive oxygen species (ROS), which was inhibited by indomethacin. Compared with the control group, SPAK knockdown MLE-12 cells had higher transepithelial electrical resistance and lower transwell monolayer permeability after hyperoxic exposure. The expression of claudin-18 was suppressed by hyperoxia, and down-regulation of SPAK restored the expression of claudin-18. The process of SPAK suppressing the expression of claudin-18 and impairing the barrier function was mediated by p38 mitogen-activated protein kinase (MAPK). Conclusions Hyperoxia up-regulates the SPAK-p38 MAPK signal pathway by ROS, which disrupts the TJ of the alveolar epithelium by suppressing the expression of claudin-18. The down-regulation of SPAK attenuates this process and protects the alveolar epithelium against the barrier dysfunction induced by hyperoxia.

Inhibition of VEGF mRNA by 2′-O,4′−C−Ethylene-Bridgednucleicacids (ENA®) Antisense Oligonucleotides and Their Influence on Off-Target Gene Expressions

2006 ◽  
Vol 25 (4-6) ◽  
pp. 503-521 ◽  
Author(s):  
Koji Morita ◽  
Koji Yamate ◽  
Shin-ichi Kurakata ◽  
Koji Abe ◽  
Kenji Watanabe ◽  
...  
Keyword(s):  
Antisense Oligonucleotides ◽  
Target Gene ◽  
Gene Expressions ◽  
Vegf Mrna

Nerve growth factor (NGF) content in adult rat brain tissues is several-fold higher than generally reported and is largely associated with sedimentable fractions

1996 ◽  
Vol 728 (1) ◽  
pp. 47-56 ◽  
Author(s):  
Marius C. Hoener ◽  
Eleanore Hewitt ◽  
James M. Conner ◽  
James W. Costello ◽  
Silvio Varon
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Rat Brain ◽  
Nerve Growth ◽  
Adult Rat ◽  
Adult Rat Brain ◽  
Brain Tissues

Determination of Protopine in Rat Brain Tissues by RRLC-ESI/Q-TOF-MS Method

2014 ◽  
Vol 6 (2) ◽  
pp. 125-130 ◽  
Author(s):  
Pei-xiang Wang ◽  
Yong-hui Li ◽  
Yan-jing Li ◽  
Ting Geng ◽  
Ming-li Li ◽  
...  
Keyword(s):  
Rat Brain ◽  
Tof Ms ◽  
Brain Tissues

Effect of silencing NEK2 on biological behaviors of HepG2 in human hepatoma cells and MAPK signal pathway

Tumor Biology ◽  
2015 ◽  
Vol 37 (2) ◽  
pp. 2023-2035 ◽  
Author(s):  
Mei-Xia Zhang ◽  
Xi-Ming Xu ◽  
Peng Zhang ◽  
Na-Na Han ◽  
Jun-Jian Deng ◽  
...  
Keyword(s):  
Hepatoma Cells ◽  
Signal Pathway ◽  
Human Hepatoma ◽  
Human Hepatoma Cells ◽  
Mapk Signal Pathway

scholarly journals Decrease in stathmin expression by arsenic trioxide inhibits the proliferation and invasion of osteosarcoma cells via the MAPK signal pathway

2017 ◽  
Vol 14 (2) ◽  
pp. 1333-1340 ◽  
Author(s):  
Tao Feng ◽  
Jun Xu ◽  
Ping He ◽  
Yuanyuan Chen ◽  
Ruiying Fang ◽  
...  
Keyword(s):  
Arsenic Trioxide ◽  
Signal Pathway ◽  
Osteosarcoma Cells ◽  
Mapk Signal Pathway ◽  
Proliferation And Invasion

Gene expressions of ubiquitin and hsp70 following focal ischaemia in rat brain

Neuroreport ◽  
1997 ◽  
Vol 8 (5) ◽  
pp. 1239-1241 ◽  
Author(s):  
Masashi Noga ◽  
Takashi Hayashi ◽  
Juni Tanaka
Keyword(s):  
Rat Brain ◽  
Gene Expressions ◽  
Focal Ischaemia
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