Medicinal plantsused in treatment of diseases earlier times, are potential sources of new drugs. In this study, chemical compositions of Echium khuzestanicum, Marrubium anisodan and Echinops cephalotes were identified by Gas Chromatography-Mass Spectrometry(GC/MS).M.anisodanand E.cephalotes aerial organs and E.khuzistanicum flowers were extracted by maceration method. According to the results, 46 kinds of chemical compounds found in methanol extract of E.khuzestanicum. There are mucilage, fatty acids, flavonoid and diterpenes in the flower of E.khuzestanicum. Seventy sixkinds of chemical compounds were found in methanol extract of E.cephalotesincludingaldehydes (7.9%), phenols (7.5%), fatty acids (5.8%) and furfural (5.4%)and86 kinds of chemical compounds found in M.anisodan extract. Furfural, steroids, vitamin B and flavonoids are the main compounds of M.anisodan.