Antibiotic resistance patterns of Escherichia coli urinary isolates and comparison with antibiotic consumption data over 10 years, 2005–2014

2017 ◽  
Vol 186 (3) ◽  
pp. 733-741 ◽  
Author(s):  
P. J. Stapleton ◽  
D. J. Lundon ◽  
R. McWade ◽  
N. Scanlon ◽  
M. M. Hannan ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Antibiotic Consumption ◽  
Consumption Data ◽  
Resistance Patterns ◽  
Antibiotic Resistance Patterns ◽  
Urinary Isolates

Occurrence of Enteropathogenic Escherichia coli Serotypes in Butter

1987 ◽  
Vol 50 (10) ◽  
pp. 829-831 ◽  
Author(s):  
F. M. ABBAR ◽  
M. TAHIR MOHAMED
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Fecal Coliforms ◽  
Enteropathogenic Escherichia Coli ◽  
Heat Stable ◽  
Resistance Patterns ◽  
Antibiotic Resistance Patterns

A total of 30 samples of butter analysed during the course of the investigation showed that fecal coliforms were absent from only 13.3% of samples. One hundred forty colonies of fecal coliforms were biochemically characterized with the following types obtained (Escherichia sp. 41.4%, Enterobacter sp. 25.7%, Citrobacter sp. 20%, Klebsiella sp. 10%). Five different serotypes, namely 0 125 K70(2), 0 142K86(1), 0 127K63(1), 0 114 K90(2), 0 111 K58(1) were detected in 7 of 58 Escherichia coli isolates and 51 strains were untypable. Three strains produced heat stable (ST) enterotoxin and belonged to the enteropathogenic serotype. The antibiotic resistance patterns of coliform strains are presented.

scholarly journals Molecular Detection of Avian Pathogenic Escherichia coli (APEC) for the First Time in Layer Farms in Bangladesh and Their Antibiotic Resistance Patterns

2020 ◽  
Vol 8 (7) ◽  
pp. 1021 ◽  
Author(s):  
Samina Ievy ◽  
Md. Saiful Islam ◽  
Md. Abdus Sobur ◽  
Mithun Talukder ◽  
Md. Bahanur Rahman ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Virulence Genes ◽  
Molecular Detection ◽  
Avian Pathogenic Escherichia Coli ◽  
Internal Organs ◽  
Pathogenic Escherichia Coli ◽  
Resistance Patterns ◽  
Antibiotic Resistance Patterns ◽  
First Time

Avian pathogenic Escherichia coli (APEC) causes significant economic losses in poultry industries. Here, we determined for the first time in Bangladesh, the prevalence of APEC-associated virulence genes in E. coli isolated from layer farms and their antibiotic resistance patterns. A total of 99 samples comprising internal organs, feces, and air were collected from 32 layer farms. Isolation was performed by culturing samples on eosin–methylene blue agar plates, while the molecular detection of APEC was performed by PCR, and antibiograms were performed by disk diffusion. Among the samples, 36 were positive for the APEC-associated virulence genes fimC, iucD, and papC. Out of 36 isolates, 7, 18, and 11 were positive, respectively, for three virulence genes (papC, fimC, and iucD), two virulence genes, and a single virulence gene. Although the detection of virulence genes was significantly higher in the internal organs, the air and feces were also positive. The antibiograms revealed that all the isolates (100%) were resistant to ampicillin and tetracycline; 97.2%, to chloramphenicol and erythromycin; 55.5%, to enrofloxacin; 50.0%, to norfloxacin and ciprofloxacin; 19.4%, to streptomycin; 11.1%, to colistin; and 8.33%, to gentamicin. Interestingly, all the isolates were multidrug-resistant (MDR). Spearman’s rank correlation coefficient analysis revealed the strongest significant correlation between norfloxacin and ciprofloxacin resistance. This is the first study in Bangladesh describing the molecular detection of APEC in layer farms. Isolated APEC can now be used for detailed genetic characterization and assessing the impact on public health.

scholarly journals 1602. Antibiotic Resistance Patterns of Clinical Escherichia coli Urinary Isolates by Outpatient Practice Type

2019 ◽  
Vol 6 (Supplement_2) ◽  
pp. S584-S584
Author(s):  
Lauren Frisbie ◽  
Scott Weissman ◽  
Hema Kapoor ◽  
Marisa A D’Angeli ◽  
Ann Salm ◽  
...  
Keyword(s):  
Logistic Regression ◽  
Antibiotic Resistance ◽  
Antibiotic Stewardship ◽  
Prescribing Practices ◽  
Patient Morbidity ◽  
Resistance Patterns ◽  
Outpatient Practice ◽  
Outpatient Settings ◽  
Antibiotic Resistance Patterns ◽  
Urinary Isolates

Abstract Background Antibiotic-resistant E. coli (EC) infections represent a major cause of morbidity and mortality, and pose a challenge to antibiotic stewardship. At present, clinicians in outpatient facilities may not have access to local antibiogram data to guide stewardship. Additionally, antibiotic resistance may vary between types of outpatient practices. Methods Using the database of a major clinical reference lab, this study analyzed several years of antibiotic susceptibility results for outpatient urinary EC isolates from Washington State. We compared rates of resistance to antibiotics between different types of outpatient practices, categorized using a modification of published ambulatory practice categories. Logistic regression was used to examine the association of outpatient practice type with antibiotic resistance, controlling year, sex, and age. Results After adjusting for year, sex, and age, logistic regression found significantly higher odds of resistance in urology compared with the reference groups of general family practice for ampicillin (OR 1.35), ciprofloxacin (OR 2.27), trimethoprim-sulfa (OR 1.51) and gentamicin (OR 1.73). We also saw increased odds of resistance to ciprofloxacin in patients from an oncology clinic (OR 1.56) as well as patients from “All other specialties” (OR 1.37). A lower odds of resistance was found in OBGYN clinics for ampicillin (OR 0.86), trimethoprim-sulfa (0.81) while a greater odds or resistance in OBGYN clinics was found for nitrofurantoin (OR 1.36). Conclusion Antibiotic resistance in EC urinary isolates can vary across types of outpatient practices according to clinical practice type. This may reflect differences in patient morbidity and/or differences in antibiotic stewardship practices and deserves further investigation. Patients with recurrent cases of resistant UTIs are generally referred to a urologist, and this was reflected in our data as there a higher odds of resistance was found in urology clinics. Similarly, we found higher odds of resistance into nitrofurantoin, a commonly prescribed antibiotic for UTIs in pregnant women, in OBGYN clinics that may reflect prescribing practices. Use of clinical data to create facility and specialty-specific antibiograms in outpatient settings may enable improved and “precise” antibiotic stewardship. Disclosures All authors: No reported disclosures.

scholarly journals Antibiotic Resistance Patterns of Diverse Escherichia coli Phylogenetic Groups Isolated from the Al-Hillah River in Babylon Province, Iraq

2019 ◽  
Vol 2019 ◽  
pp. 1-8 ◽  
Author(s):  
Mourouge Saadi Alwash ◽  
Hawraa Mohammed Al-Rafyai
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Anthropogenic Activities ◽  
Health Concern ◽  
Resistant Bacteria ◽  
Anthropogenic Pressures ◽  
Phylogenetic Groups ◽  
E Coli ◽  
Resistance Patterns ◽  
Antibiotic Resistance Patterns

Surface water contamination remains a major worldwide public health concern and may contribute to the dissemination of antibiotic-resistant bacteria. The Al-Hillah River in the city of Babylon Province, Iraq, diverts flows from the Euphrates River. Because of its importance in irrigation and population density, it faces several forced and unforced changes due to anthropogenic activities. To evaluate water quality, water samples were collected from three sites with different anthropogenic pressures along the Al-Hillah River. These samples were subjected to bacteriological analyses, i.e., total coliforms, Escherichia coli, and faecal enterococci. The phylogenetic groups of the E. coli isolates (n = 61) were typed by rapid PCR-based analyses. Representatives of each isolate were tested phenotypically for resistance to six classes of antibiotics and characterized according to their phylogenetic groups. The results demonstrated the highest resistance levels were to β-lactam antibiotics, followed by fosfomycin and aminoglycosides. Escherichia coli isolates belonging to phylogenetic groups A and B2 were the most common and were characterized by a higher prevalence of antibiotic resistance. This study is important for understanding the current conditions of the Al-Hillah River, as the data reveal a high prevalence of multiresistance among E. coli isolates circulating at the three sampling sites.

Sign in / Sign up

Export Citation Format

Share Document