Bi-layer Gelatin Film: Activating Film by Incorporation of “Pitanga” Leaf Hydroethanolic Extract and/or Nisin in the Second Layer

The seeds of Ammodaucus leucotrichus Cosson and Durieu have been used in the North African Sahara as a traditional medicine to treat diabetes. The present study investigates the antidiabetic, antihyperglycemic, and anti-inflammatory properties of the defatted hydroethanolic extract of Ammodaucus leucotrichus (DHEAM). The antidiabetic and the antihyperglycemic studies were assessed on alloxan-induced diabetic with orally administered doses of DHEAM (100 and 200 mg/kg). At the same time, its anti-inflammatory propriety was evaluated by measuring edema development in the Wistar rats paw induced with carrageenan. Treatment of diabetic mice with DHEAM for four weeks managed their high fasting blood glucose levels, improved their overall health, and also revealed an excellent antihyperglycemic activity. Following the anti-inflammatory results, DHEAM exhibited a perfect activity. HPLC results revealed the presence of seven molecules (chlorogenic acid, 3-p-coumaroylquinic acid, gallic acid, ferulic acid, myricetin, quercetin, luteolin). This work indicates that the DHEAM has an important antidiabetic, antihyperglycemic, and anti-inflammatory effect that can be well established as a phytomedicine to treat diabetes.

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Characterization, antioxidant and antibacterial activities of gelatin film incorporated with protocatechuic acid and its application on beef preservation

LWT ◽

10.1016/j.lwt.2021.112154 ◽

2021 ◽

pp. 112154

Author(s):

Chan Zhong ◽

Peng-Fei Hou ◽

Ya-Xing Li ◽

Wen-Yu Yang ◽

Mei Shu ◽

...

Keyword(s):

Protocatechuic Acid ◽

Antibacterial Activities ◽

Gelatin Film

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Effect of Vernonia cognata on oxidative damage induced by ethanol in rats

Human & Experimental Toxicology ◽

10.1177/0960327110377646 ◽

2010 ◽

Vol 30 (7) ◽

pp. 675-684 ◽

Cited By ~ 10

Author(s):

CS Mota ◽

RB Freitas ◽

ML Athayde ◽

AA Boligon ◽

PR Augusti ◽

...

Keyword(s):

Oxidative Stress ◽

High Performance ◽

Tissue Injury ◽

Antioxidant Effect ◽

Gastric Tissue ◽

Thiol Groups ◽

Protein Thiol ◽

Stress Markers ◽

Hydroethanolic Extract ◽

And Oxidative Stress

Free radicals production and oxidative stress play a central role in injuries caused by ethanol (EtOH) on gastric mucosal. Thus, strategies to counteract EtOH toxicity are highly desirable. This study was aimed at evaluating whether Vernonia cognata extract would reduce EtOH effects in rats. Rats received Vernonia cognata extract (0, 1 and 2 g/kg bw, by gavage) 1 hour after EtOH had been administered (0 or 70%, 0.5 mL/100 g bw, by gavage) and were killed 1 hour after Vernonia cognata extract administration. The stomach was removed for macroscopic and histopathological evaluation, as well as, oxidative stress markers such as lipoperoxidation (LPO) and non-protein thiol groups (NPSH) levels and catalase (CAT) activity. EtOH acute exposure increased LPO and decreased NPSH levels and CAT activity along with macroscopic and microscopic lesions in gastric tissue, confirming the involvement of oxidative stress in EtOH toxicity. Vernonia cognata extract attenuated oxidative and histopathological features induced by EtOH at all evaluated doses. Moreover, both studied doses of Vernonia cognata extract caused an increase in NPSH levels per se. However, only the dose of 2 g/kg reverted all macroscopic changes caused by EtOH toxicity. The protective effect of the extract could be attributed to antioxidant molecules present in the extract, such as flavonoids and phenolic acids, which were quantified by high performance liquid chromatography (HPLC). Thus, an antioxidant effect of the extract leads to a protection on gastric tissue. Our results indicate that Vernonia cognata hydroethanolic extract could have a beneficial role against EtOH toxicity by preventing oxidative stress and gastric tissue injury.

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Safety Assessment of a Hydroethanolic Extract of Caralluma fimbriata

International Journal of Toxicology ◽

10.1177/1091581813492827 ◽

2013 ◽

Vol 32 (5) ◽

pp. 385-394 ◽

Cited By ~ 3

Author(s):

Antoinette Y. Odendaal ◽

Narendra S. Deshmukh ◽

Tennille K. Marx ◽

Alexander G. Schauss ◽

John R. Endres ◽

...

Keyword(s):

Developmental Toxicity ◽

Toxicity Study ◽

Developmental Study ◽

Sprague Dawley ◽

Oral Toxicity ◽

Toxicological Assessment ◽

Sprague Dawley Rats ◽

Chromosomal Aberration Test ◽

Hydroethanolic Extract

This toxicological assessment evaluated the safety of a hydroethanolic extract prepared from Caralluma fimbriata (CFE), a dietary supplement marketed worldwide as an appetite suppressant. Studies included 2 in vitro genotoxicity assays, a repeated dose oral toxicity study, and a developmental study in rats. No evidence of in vitro mutagenicity or clastogenicity surfaced in the in vitro studies at concentrations up to 5000 μg of extract/plate (Ames test) or 5000 μg of extract/mL (chromosomal aberration test). No deaths or treatment-related toxicity were seen in the 6-month chronic oral toxicity study in Sprague-Dawley rats conducted at 3 doses (100, 300, and 1000 mg/kg body weight (bw)/d). The no observed effect level for CFE in this study was considered to be 1000 mg/kg bw/d. A prenatal developmental toxicity study conducted at 3 doses (250, 500, and 1000 mg/kg bw/d) in female Sprague-Dawley rats resulted in no treatment-related external, visceral, or skeletal fetal abnormalities, and no treatment-related maternal or pregnancy alterations were seen at and up to the maximum dose tested. CFE was not associated with any toxicity or adverse events.

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