scholarly journals Defatted Hydroethanolic Extract of Ammodaucus leucotrichus Cosson and Durieu Seeds: Antidiabetic and Anti-Inflammatory Activities

2020 ◽  
Vol 10 (24) ◽  
pp. 9147
Author(s):  
Imane Es-Safi ◽  
Hamza Mechchate ◽  
Amal Amaghnouje ◽  
Anna Calarco ◽  
Smahane Boukhira ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Fasting Blood Glucose ◽  
Diabetic Mice ◽  
North African ◽  
Glucose Levels ◽  
The North ◽  
Blood Glucose Levels ◽  
Anti Inflammatory ◽  
Hydroethanolic Extract ◽  
Inflammatory Effect

The seeds of Ammodaucus leucotrichus Cosson and Durieu have been used in the North African Sahara as a traditional medicine to treat diabetes. The present study investigates the antidiabetic, antihyperglycemic, and anti-inflammatory properties of the defatted hydroethanolic extract of Ammodaucus leucotrichus (DHEAM). The antidiabetic and the antihyperglycemic studies were assessed on alloxan-induced diabetic with orally administered doses of DHEAM (100 and 200 mg/kg). At the same time, its anti-inflammatory propriety was evaluated by measuring edema development in the Wistar rats paw induced with carrageenan. Treatment of diabetic mice with DHEAM for four weeks managed their high fasting blood glucose levels, improved their overall health, and also revealed an excellent antihyperglycemic activity. Following the anti-inflammatory results, DHEAM exhibited a perfect activity. HPLC results revealed the presence of seven molecules (chlorogenic acid, 3-p-coumaroylquinic acid, gallic acid, ferulic acid, myricetin, quercetin, luteolin). This work indicates that the DHEAM has an important antidiabetic, antihyperglycemic, and anti-inflammatory effect that can be well established as a phytomedicine to treat diabetes.

scholarly journals Antioxidant, Anti-Inflammatory and Antidiabetic Proprieties of LC-MS/MS Identified Polyphenols from Coriander Seeds

Molecules ◽  
2021 ◽  
Vol 26 (2) ◽  
pp. 487
Author(s):  
Hamza Mechchate ◽  
Imane Es-safi ◽  
Amal Amaghnouje ◽  
Smahane Boukhira ◽  
Amal A. Alotaibi ◽  
...  
Keyword(s):  
Fasting Blood Glucose ◽  
Wistar Rat ◽  
Coriandrum Sativum ◽  
Oral Glucose ◽  
Diabetic Mice ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Antihyperlipidemic Activity ◽  
Anti Inflammatory ◽  
Antioxidant Effects

Coriandrum sativum L. seeds are traditionally used to treat diabetes and its complications (inflammation and formation of reactive oxygen species) around the world. The present study investigates the antidiabetic, anti-inflammatory, and antioxidant effects of the polyphenol fraction of Coriandrum sativum seeds (PCS). Diabetic mice were orally administered with PCS (25 and 50 mg/kg b.w.) for 28 days. Oral glucose tolerance (OGTT) was also evaluated along with the anti-inflammatory effect, assessed by measuring paw edema development induced with carrageenan in Wistar rat and the antioxidant activity assessed using two tests (β-carotene discoloration and DPPH). Treatment of diabetic mice with PCS for four weeks managed their high fasting blood glucose levels, improved their overall health, also revealed an excellent antihyperlipidemic activity. The OGTT result showed a potent antihyperglycemic activity, and following the anti-inflammatory and antioxidant effects, the PCS exhibited a perfect activity. LC-MS/MS result revealed the presence of 9 polyphenols. This modest work indicates that the PCS have an important antidiabetic, antihyperglycemic, antihyperlipidemic, anti-inflammatory, and antioxidant effect that can be well established treatment of diabetes and its complications.

scholarly journals Effect of reduced dietary zinc intake on carbohydrate and Zn metabolism in the genetically diabetic mouse (C57BL/KsJ db+/db+)

1988 ◽  
Vol 60 (3) ◽  
pp. 499-507 ◽  
Author(s):  
Susan Southon ◽  
Z. Kechrid ◽  
A. J. A. Wright ◽  
Susan J. Fairweather-Tait
Keyword(s):  
Blood Glucose ◽  
Control Diet ◽  
Fasting Blood Glucose ◽  
Liver Glycogen ◽  
Diabetic Mouse ◽  
Whole Body ◽  
Diabetic Mice ◽  
Control Groups ◽  
Glucose Levels ◽  
Blood Glucose Levels

1. Male, 4–5-week-old, genetically diabetic mice (C57BL/KsJ db/db) and non-diabetic heterozygote litter-mates (C57BL/KsJ db/+)were fed on a diet containing 1 mg zinc/kg (low-Zn groups) or 54 mg Zn/kg (control groups) for 27 d. Food intakes and body-weight gain were recorded regularly. On day 28, after an overnight fast, animals were killed and blood glucose and insulin concentrations, liver glycogen, and femur and pancreatic Zn concentrations were determined.2. The consumption of the low-Zn diet had only a minimal effect on the Zn status of the mice as indicated by growth rate, food intake and femur and pancreatic Zn concentrations. In fact, diabetic mice fed on the low-Zn diet had a higher total food intake than those fed on the control diet. The low-Zn diabetic mice had higher fasting blood glucose and liver glycogen levels than their control counterparts. Fasting blood insulin concentration was unaffected by dietary regimen.3. A second experiment was performed in which the rate of loss of 65Zn, injected subcutaneously, was measured by whole-body counting in the two mouse genotypes over a 28 d period, from 4 to 5 weeks of age. The influence of feeding low-Zn or control diets was also examined. At the end of the study femur and pancreatic Zn and non-fasting blood glucose levels were determined.4. All mice fed on the low-Zn diet showed a marked reduction in whole-body 65Zn loss compared with those animals fed on the control diet. In the low-Zn groups, the loss of 65Zn from the diabetic mice was significantly greater than that from heterozygote mice. This difference was not observed in the control groups. Blood glucose levels were elevated in the low-Zn groups. Possible reasons for these observations are discussed.5. The present study demonstrates an adverse effect of reduced dietary Zn intake on glucose utilization in the genetically diabetic mouse, which occurred before any significant tissue Zn depletion became apparent.

scholarly journals HYPOGLYCEMIC EFFECT OF ANDROGRAPHIS ECHIOIDES ON STREPTOZOTOCIN INDUCED EXPERIMENTAL RATS

2015 ◽  
Vol 7 ◽  
pp. 55
Author(s):  
Mani Rupeshkumar ◽  
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Wistar Rats ◽  
Methanol Extract ◽  
Fasting Blood Glucose ◽  
Hypoglycemic Effect ◽  
Reference Drug ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Single Intraperitoneal Injection

The present study aims to study the hypoglycemic effect of methanol extract of Andrographisechioides (MEAE) in streptozotocin (STZ)-induced diabetic Wistar rats. Hyperglycemia was induced in rats by single intraperitoneal injection of STZ (55 mg/kg bodyweight). Three days after STZ induction, the hyperglycemic rats were treated with MEAE orally at the doses of 200, 500, and 800 mg/kg body weight daily for 21 days. Glibenclamide (1 mg/kg, orally) was used as reference drug. The fasting blood glucose levels were measured on each 7th day during the 21 days of treatment.

scholarly journals Red dragon fruit (Hylocereus spp.) peel marmalade effectively improve blood glucose and lipid profile of hypercholesterolemic wistar rats

2020 ◽  
Vol 9 (1) ◽  
pp. 61-67
Author(s):  
Natalia Desy Putriningtyas ◽  
Intan Permatasari ◽  
Dita Oktaviani ◽  
Anastasia Servia Raha ◽  
Siti Wahyuningsih
Keyword(s):  
Blood Glucose ◽  
Functional Food ◽  
Wistar Rats ◽  
Fasting Blood Glucose ◽  
Control Group ◽  
Fruit Peel ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Dragon Fruit ◽  
Significant Difference

Background: Polyphenols, antioxidants, dietary fiber, and vitamin contained in the red dragon fruit peel. Red dragon fruit peel can be processed into marmalade. Red dragon fruit peel marmalade has the potential to be a functional food. Functional food is food that has a physiological function based on scientific studies.Objectives: The objective of this study is to analyze the effect of red dragon fruit peel marmalade on fasting blood glucose levels, HDL, LDL, and triglycerides levels of hypercholesterolemic Wistar rats.Methods: This study used a pre-&post-test control group design. Hypercholesterolemic male Wistar rats were randomly assigned into five groups. Hypercholesterolemia was induced by 1% cholesterol powder and 0.5% cholic acid for two weeks. All groups received standard chow. Samples were grouped into five groups: K-; K+; K1 (0.94 g/kg b.wt/day); K2 (1.41 g/kg b.wt/day); K3 (1.88 g/kg b.wt/day). The intervention was carried out for 28 days. GDP level was measured using the GOD-PAP. HDL, LDL, and triglyceride were analyzed with spectrophotometry. GDP, HDL, LDL, and triglyceride levels were measured twice before fasting. A paired t-test and one-way ANOVA were used to analyze the data.Results: The result showed that K-; K1; K2; K3 had a significant difference between groups before and after the intervention (p<0.05). Red dragon fruit peel Marmalade was able to reduce the levels of GDP, LDL, triglycerides, and increase HDL (p <0.05).Conclusion: Red dragon fruit peel marmalade reduced fasting blood glucose levels, LDL, triglyceride levels, and increased HDL levels of hypercholesterolemic Wistar rats.

scholarly journals The Effect of Belimbing Wuluh (Averrhoa Bilimbi L.) and Red Guava (Psidium Guajava L.) Extract on Fasting Blood Glucose Levels of Diabetic Wistar Rats Model

2019 ◽  
Vol 5 (2) ◽  
pp. 57
Author(s):  
Yesi Warisman ◽  
Arinda Lironika Suryana ◽  
Zora Olivia
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Wistar Rats ◽  
Fasting Blood Glucose ◽  
Diabetic Rats ◽  
Control Group ◽  
Standard Diet ◽  
Glucose Levels ◽  
Blood Glucose Levels

Diabetes mellitus is a metabolic disease with special characteristic which increased blood glucose level (hiperglicemic), its happens because of the abnormality of insulin secretion, insulin mechanisme or both of them. Belimbing wuluh and red guava extract can become alternative functional drink that contain rich of vitamin C to help controlling blood glucose level. The aimed of this study was to determined the effect of belimbing wuluh and red guava extract on blood glucose levels of Rattus novergicus strain Wistar diabetic model. This research was an true experimental study with a randomized post-test control group design. Twentyfour (24) male wistar strain rats weighing 200-250 grams, aged 2-3 months, were divided into 4 groups. The control group (K-) non-diabetic rats, the control group (K+) diabetic rats given standard diet, and the treatment group diabetic rats given standard diet and belimbing wuluh and red guava extract intervetion doses 10,7 ml/day (P1) and 21ml/day (P2). Measurement of pretest posttest fasting blood glucose levels was carried out using biolyzer. The data were analyzed by One Way Anova test. The results showed that there were significant differences in delta pretest and posttest fasting blood glucose levels between all groups. Blood glucose levels decreased at 55.6%. In conclusion, belimbing wuluh and red guava extract 21ml/day most effectively decreased fasting blood glucose levels in diabetic wistar rats moldel.   Keywords: belimbing wuluh, blood glucose level, diabetes mellitus, red guava

scholarly journals Cadmium Exposure Decreases Fasting Blood Glucose Levels and Exacerbates Type-2 Diabetes in a Mouse Model

2021 ◽  
Author(s):  
Mengyang Li ◽  
Shuai Wang ◽  
Xiuxiu Liu ◽  
Zhijie Sheng ◽  
Bingyan Li ◽  
...  
Keyword(s):  
Type 2 Diabetes ◽  
Blood Glucose ◽  
Fasting Blood Glucose ◽  
Diabetic Group ◽  
Diabetic Mice ◽  
Hepatic Enzymes ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Cd Exposure

Abstract Purpose Although the effects of cadmium (Cd) on the development of diabetes have been extensively investigated, the relationship between Cd exposure and the severity of established diabetes is unclear. Herein, we investigate the effects of long-term exposure to Cd in a streptozotocin-induced mouse model of type 2 diabetes and the underlying mechanism. Methods C57BL/6 Mice were divided into the following four groups: 1) control group; 2) Cd-exposed group; 3) diabetic group; 4) Cd-exposed diabetic group. Cd exposure was established by the administration of 155 ppm CdCl2 in drinking water. After 25 weeks of treatment, serum fasting glucose and insulin were measured. Meanwhile, the liver and pancreas specimens were sectioned and stained with Hematoxylin and eosin. Gluconeogenesis, glycolysis, lactate concentration and fibrosis in liver were evaluated. Results Clinical signs attributable to diabetes were more apparent in Cd-exposed diabetic mice. Interestingly, Cd exposure significantly decreased fasting blood glucose levels in diabetic group. We further demonstrated that the glycolysis related hepatic enzymes, pyruvate kinase M2 (PKM-2) and lactic dehydrogenase A (LDHA) were both increased, while the gluconeogenesis related hepatic enzymes, phosphoenolpyruvate-1 (PCK-1) and glucose-6-phosphatase (G6Pase) were both decreased in Cd exposed diabetic mice, indicating that Cd increased glycolysis and inhibited gluconeogenesis in diabetic model. Moreover, lactate accumulation was noted accompanied by the increased inflammation and fibrosis in the livers of diabetic mice following Cd exposure. Conclusions Cd exposure disturbed glucose metabolism and exacerbated diabetes, providing a biological relevance that DM patients are at greater risk when exposed to Cd.

Caveolin gene transfer improves glucose metabolism in diabetic mice

2010 ◽  
Vol 298 (3) ◽  
pp. C450-C456 ◽  
Author(s):  
Koji Otsu ◽  
Yoshiyuki Toya ◽  
Jin Oshikawa ◽  
Reiko Kurotani ◽  
Takuya Yazawa ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Glucose Metabolism ◽  
Gene Transfer ◽  
Insulin Receptor ◽  
Glycogen Synthesis ◽  
Fasting Blood Glucose ◽  
Hepatic Glycogen ◽  
Diabetic Mice ◽  
Glucose Levels ◽  
Blood Glucose Levels

Caveolin, a member of the membrane-anchoring protein family, accumulates various growth receptors in caveolae and inhibits their function. Upregulation of caveolin attenuates cellular proliferation and growth. However, the role of caveolin in regulating insulin signals remains controversial. Here, we demonstrate that caveolin potently enhances insulin receptor (IR) signaling when overexpressed in the liver in vivo. Adenovirus-mediated gene transfer was used to overexpress caveolin specifically in the liver of diabetic obese mice, which were generated with a high-fat diet. Expression of molecules involved in IR signaling, such as IR or Akt, remained unchanged after gene transfer. However, hepatic glycogen synthesis was markedly increased with a decrease in phosphoenolpyruvate carboxykinase protein expression. Insulin sensitivity was increased after caveolin gene transfer as determined by decreased blood glucose levels in response to insulin injection and fasting blood glucose levels. Glucose tolerant test performance was also improved. Similar improvements were obtained in KKA y genetically diabetic mice. Adenovirus-mediated overexpression of caveolin-3 in hepatic cells also enhanced IR signaling, as shown by increased phosphorylation of IR in response to insulin stimulation and higher glycogen synthesis at baseline. These effects were attributed mostly to increased insulin receptor activity and caveolin-mediated, direct inhibition of protein tyrosine phosphatase 1B, which was increased in obese mouse livers. In conclusion, our results suggest that caveolin is an important regulator of glucose metabolism that can enhance insulin signals.

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