Exploring the Degradation of Gallotannins Catalyzed by Tannase Produced by Aspergillus niger GH1 for Ellagic Acid Production in Submerged and Solid-State Fermentation

2017 ◽  
Vol 185 (2) ◽  
pp. 476-483 ◽  
Author(s):  
Mónica L. Chávez-González ◽  
Sylvain Guyot ◽  
Raul Rodríguez-Herrera ◽  
Arely Prado-Barragán ◽  
Cristóbal N. Aguilar
2008 ◽  
Vol 35 (6) ◽  
pp. 507-513 ◽  
Author(s):  
Armando Robledo ◽  
Antonio Aguilera-Carbó ◽  
Raúl Rodriguez ◽  
José Luis Martinez ◽  
Yolanda Garza ◽  
...  

2018 ◽  
Vol 247 ◽  
pp. 412-418 ◽  
Author(s):  
Pedro Aguilar-Zárate ◽  
Jorge E. Wong-Paz ◽  
Luis V. Rodríguez-Duran ◽  
Juan Buenrostro-Figueroa ◽  
Mariela Michel ◽  
...  

Author(s):  
Juan Buenrostro-Figueroa, Alberto Ascacio-Valdés, Leonardo Sepúlveda ◽  
Arely Prado- Barragán ◽  
Miguel Ángel Aguilar- González ◽  
Cristóbal Noé Aguilar

Ellagic acid and ellagitannins are defense phytochemicals and dietary polyphenols found in fruits and nuts and implicated with strong antioxidant, anticancer and antiatherosclerotic biological properties. Unfortunately, there are few reports on the bioconversion studies from ellagitannins to ellagic acid. The objective of the present work was to use of partially purified ellagitannins from pomegranate peels as inducers for production of ellagic acid by Aspergillus niger GH1 in solid-state fermentation on inert supports (perlite, polyurethane foam and nylon fiber). For total pomegranate polyphenols (TPP), two methodologies were compared. Higher recovery of TTP (70 g kg-1 peel) in a short time (2 h) were obtained by filtering through a muslin cloth followed by centrifugation at 3000 rpm for 15 min. Production of ellagic acid and ellagitannase enzyme were evaluated. Maximum ellagic acid production (231.22 mg g-1 TPP) at 24 h cultivation with an ellagitannase activity of 3213 U L-1 was obtained when using polyurethane foam as inert support. Polyurethane foam was the best solid support to produce high yields of ellagic acid production.


2005 ◽  
Vol 48 (spe) ◽  
pp. 29-36 ◽  
Author(s):  
Flávera Camargo Prado ◽  
Luciana Porto de Souza Vandenberghe ◽  
Carlos Ricardo Soccol

The aim of this work was to study the relation between citric acid production and respiration of Aspergillus niger LPB 21 in solid-state fermentation of cassava bagasse. The experiments were carried out in horizontal drum bioreactor coupled with a gas chromatography system. Fermentation was conduced for 144 h with initial substrate moisture of 60% using heat-treated cassava bagasse as sole carbon source. The exhausted air from the bioreactor was analyzed for the monitoring of CO2 produced and O2 consumed in order to estimate the biomass biosynthesis by the fungal culture. The metabolic activity of A. niger growth was associated to citric acid production. The system using FERSOL software determined 4.372 g of biomass/g of consumed O2. Estimated and analytically determined biomass values followed the same pattern showing that the applied mathematical model was adapted.


2012 ◽  
Vol 47 (12) ◽  
pp. 2199-2203 ◽  
Author(s):  
L. Sepúlveda ◽  
A. Aguilera-Carbó ◽  
J.A. Ascacio-Valdés ◽  
R. Rodríguez-Herrera ◽  
J.L. Martínez-Hernández ◽  
...  

Plants ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 1518
Author(s):  
De León-Medina José Carlos ◽  
Sepúlveda Leonardo ◽  
Morlett-Chávez Jesús ◽  
Meléndez-Renteria Paola ◽  
Zugasti-Cruz Alejandro ◽  
...  

This work was performed to study Castilla Rose (Purshia plicata) as a potential source of polyphenols obtained by solid-state fermentation (SSF)-assisted extraction using the microorganism Aspergillus niger GH1 and to evaluate the antioxidant activity of the extracted compounds. First, water absorption capacity (WAC) of the plant material, radial growth of the microorganism, determination of best fermentation conditions, and maximum accumulation time of polyphenols were tested. Then, a larger-scale fermentation, polyphenols isolation by column liquid chromatography (Amberlite XAD-16) and recovered compounds identification by HPLC-MS were made. Finally, the antioxidant activity of the recovered compounds was tested by ABTS, DPPH, and lipid oxidation inhibition assays. The best fermentation conditions were temperature 25 °C and inoculum 2 × 106 spores/g, while the maximum extraction time of polyphenols was 24 h (173.95 mg/g). The HPLC/MS analysis allowed the identification of 25 different polyphenolic compounds, and the antioxidant activity of the obtained polyphenols was demonstrated, showing ABTS assay the most effective with inhibition of 94.34%.


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