On-line monitoring of Aspergillus niger GH1 growth in a bioprocess for the production of ellagic acid and ellagitannase by solid-state fermentation

This work was performed to study Castilla Rose (Purshia plicata) as a potential source of polyphenols obtained by solid-state fermentation (SSF)-assisted extraction using the microorganism Aspergillus niger GH1 and to evaluate the antioxidant activity of the extracted compounds. First, water absorption capacity (WAC) of the plant material, radial growth of the microorganism, determination of best fermentation conditions, and maximum accumulation time of polyphenols were tested. Then, a larger-scale fermentation, polyphenols isolation by column liquid chromatography (Amberlite XAD-16) and recovered compounds identification by HPLC-MS were made. Finally, the antioxidant activity of the recovered compounds was tested by ABTS, DPPH, and lipid oxidation inhibition assays. The best fermentation conditions were temperature 25 °C and inoculum 2 × 106 spores/g, while the maximum extraction time of polyphenols was 24 h (173.95 mg/g). The HPLC/MS analysis allowed the identification of 25 different polyphenolic compounds, and the antioxidant activity of the obtained polyphenols was demonstrated, showing ABTS assay the most effective with inhibition of 94.34%.

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BIOCONVERSION OF ELLAGITANNINS TO ELLAGIC ACID FROM POMEGRANATE PEELS BY SOLID-STATE FERMENTATION USING ASPERGILLUS NIGER AND RHIZOPUS ORYZAE

10.36106/paripex/1701247 ◽

2020 ◽

pp. 1-4

Author(s):

R. Sundaralingam ◽

S. Premina ◽

S. Niren Andrew

Keyword(s):

Solid State ◽

Aspergillus Niger ◽

Solid State Fermentation ◽

Ellagic Acid ◽

Fruits And Vegetables ◽

Rhizopus Oryzae ◽

Fermentation Broth ◽

Antioxidant Properties ◽

Mixed Cultures ◽

Test Organisms

Ellagic acid an effective polyphenol antioxidant found in many fruits and vegetables. The antioxidant properties of ellagic acid have incited preliminary research into the impending health benefits of ellagic acid consumption. In the present research, an attempt is made to extract ellagic acid from pomegranate peels using solid state cultures of Aspergillus niger, Rhizopus oryzae and mixed cultures of both. The fungus has the potential to convert ellagitannins in pomegranate to ellagic acid as an intermediary metabolite. Test organisms were isolated from soil, identified and screened for production of tannase enzyme. Solid state fermentation of pomegranate husk (10% w/v) using A. niger, R. oryzae and mixed cultures was performed for the production of ellagic acid. Samples were collected daily from the fermentation broth and physico-chemical analyses like changes in the total tannin content and tannase enzyme assay were performed. After fermentation the production of ellagic acid in the medium was determined using HPLC. The amount of ellagic acid produced was 9.1mg (for A.niger), 79.1mg (for R. oryzae), 69.6mg (for mixed culture of both A. niger and R.oryzae).

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Ellagic acid production by solid-state fermentation influenced by the inert solid supports

Emirates Journal of Food and Agriculture ◽

10.9755/ejfa.2018.v30.i9.1796 ◽

2018 ◽

pp. 750

Author(s):

Juan Buenrostro-Figueroa, Alberto Ascacio-Valdés, Leonardo Sepúlveda ◽

Arely Prado- Barragán ◽

Miguel Ángel Aguilar- González ◽

Cristóbal Noé Aguilar

Keyword(s):

Solid State ◽

Polyurethane Foam ◽

Solid State Fermentation ◽

Ellagic Acid ◽

Acid Production ◽

Biological Properties ◽

Dietary Polyphenols ◽

Aspergillus Niger Gh1 ◽

High Yields ◽

Short Time

Ellagic acid and ellagitannins are defense phytochemicals and dietary polyphenols found in fruits and nuts and implicated with strong antioxidant, anticancer and antiatherosclerotic biological properties. Unfortunately, there are few reports on the bioconversion studies from ellagitannins to ellagic acid. The objective of the present work was to use of partially purified ellagitannins from pomegranate peels as inducers for production of ellagic acid by Aspergillus niger GH1 in solid-state fermentation on inert supports (perlite, polyurethane foam and nylon fiber). For total pomegranate polyphenols (TPP), two methodologies were compared. Higher recovery of TTP (70 g kg-1 peel) in a short time (2 h) were obtained by filtering through a muslin cloth followed by centrifugation at 3000 rpm for 15 min. Production of ellagic acid and ellagitannase enzyme were evaluated. Maximum ellagic acid production (231.22 mg g-1 TPP) at 24 h cultivation with an ellagitannase activity of 3213 U L-1 was obtained when using polyurethane foam as inert support. Polyurethane foam was the best solid support to produce high yields of ellagic acid production.

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Utilization of molasses and sugar cane bagasse for production of fungal invertase in solid state fermentation using Aspergillus niger GH1

Brazilian Journal of Microbiology ◽

10.1590/s1517-83822014000200002 ◽

2014 ◽

Vol 45 (2) ◽

pp. 373-377 ◽

Cited By ~ 25

Author(s):

F. Veana ◽

J.L. Martínez-Hernández ◽

C.N. Aguilar ◽

R. Rodríguez-Herrera ◽

G. Michelena

Keyword(s):

Solid State ◽

Aspergillus Niger ◽

Solid State Fermentation ◽

Sugar Cane ◽

Sugar Cane Bagasse ◽

Aspergillus Niger Gh1

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Food Waste-Assisted Metal Extraction from Printed Circuit Boards: The Aspergillus niger Route

Microorganisms ◽

10.3390/microorganisms9050895 ◽

2021 ◽

Vol 9 (5) ◽

pp. 895

Author(s):

Carlotta Alias ◽

Daniela Bulgari ◽

Fabjola Bilo ◽

Laura Borgese ◽

Alessandra Gianoncelli ◽

...

Keyword(s):

Solid State ◽

Aspergillus Niger ◽

Food Waste ◽

Solid State Fermentation ◽

Printed Circuit Boards ◽

Electronic Waste ◽

Metal Extraction ◽

Circuit Boards ◽

Printed Circuit ◽

Waste Printed Circuit Boards

A low-energy paradigm was adopted for sustainable, affordable, and effective urban waste valorization. Here a new, eco-designed, solid-state fermentation process is presented to obtain some useful bio-products by recycling of different wastes. Urban food waste and scraps from trimmings were used as a substrate for the production of citric acid (CA) by solid state fermentation of Aspergillus niger NRRL 334, with a yield of 20.50 mg of CA per gram of substrate. The acid solution was used to extract metals from waste printed circuit boards (WPCBs), one of the most common electronic waste. The leaching activity of the biological solution is comparable to a commercial CA one. Sn and Fe were the most leached metals (404.09 and 67.99 mg/L, respectively), followed by Ni and Zn (4.55 and 1.92 mg/L) without any pre-treatments as usually performed. Commercial CA extracted Fe more efficiently than the organic one (123.46 vs. 67.99 mg/L); vice versa, biological organic CA recovered Ni better than commercial CA (4.55 vs. 1.54 mg/L). This is the first approach that allows the extraction of metals from WPCBs through CA produced by A. niger directly grown on waste material without any sugar supplement. This “green” process could be an alternative for the recovery of valuable metals such as Fe, Pb, and Ni from electronic waste.

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