Maternal Selenium Supplementation Enhanced Skeletal Muscle Development Through Increasing Protein Synthesis and SelW mRNA Levels of their Offspring

N6-methyladenosine (m6A) is the most prevalent methylation modification of eukaryotic mRNA, and it plays an important role in regulating gene expression. Previous studies have found that m6A methylation plays a role in mammalian skeletal muscle development. However, the effect of m6A on bovine skeletal myogenesis are still unclear. Here, we selected proliferating myoblasts (GM) and differentiated myotubes (on the 4th day of differentiation, DM) for m6A-seq and RNA-seq to explore the m6A methylation modification pattern during bovine skeletal myogenesis. m6A-seq analysis revealed that m6A methylation was an abundant modification of the mRNA in bovine myoblasts and myotubes. We scanned 5,691–8,094 m6A-modified transcripts, including 1,437 differentially methylated genes (DMGs). GO and KEGG analyses revealed that DMGs were primarily involved in transcriptional regulation and RNA metabolism, as well as insulin resistance and metabolic pathways related to muscle development. The combined analysis further identified 268 genes that had significant changes at both m6A and mRNA levels, suggesting that m6A modification may regulate myoblast differentiation by mediating the expression of these genes. Furthermore, we experimentally confirmed four genes related to myogenesis, including MYOZ2, TWIST1, KLF5 and MYOD1, with differential changes in both m6A and mRNA levels during bovine myoblast differentiation, indicating that they can be potential candidate targets for m6A regulation of skeletal myogenesis. Our results may provide new insight into molecular genetics and breeding of beef cattle, and provide a reference for investigating the mechanism of m6A regulating skeletal muscle development.

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Maternal Zinc Supplementation Enhanced Skeletal Muscle Development Through Increasing Protein Synthesis and Inhibiting Protein Degradation of Their Offspring

Biological Trace Element Research ◽

10.1007/s12011-014-0122-5 ◽

2014 ◽

Vol 162 (1-3) ◽

pp. 309-316 ◽

Cited By ~ 8

Author(s):

Jing Gao ◽

Zengpeng Lv ◽

Changwu Li ◽

Yunshuang Yue ◽

Xu Zhao ◽

...

Keyword(s):

Skeletal Muscle ◽

Protein Synthesis ◽

Protein Degradation ◽

Muscle Development ◽

Zinc Supplementation ◽

Skeletal Muscle Development ◽

Inhibiting Protein

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Effects of Birth Weight and Postnatal Nutritional Restriction on Skeletal Muscle Development, Myofiber Maturation, and Metabolic Status of Early-Weaned Piglets

Animals ◽

10.3390/ani10010156 ◽

2020 ◽

Vol 10 (1) ◽

pp. 156 ◽

Cited By ~ 2

Author(s):

Liang Hu ◽

Xie Peng ◽

Fei Han ◽

Fali Wu ◽

Daiwen Chen ◽

...

Keyword(s):

Skeletal Muscle ◽

Birth Weight ◽

Nutrient Intake ◽

Muscle Development ◽

Metabolic Status ◽

Nutrient Restriction ◽

Mrna Levels ◽

Skeletal Muscle Development ◽

Weaned Piglets ◽

Nutritional Restriction

Piglets with light weaning weight commonly have a slow post-weaning growth rate due to impaired skeletal muscle development. Therefore, the present study aimed to investigate the impact of birth weight and nutrient intake on skeletal muscle development, myofiber maturation, and metabolic status of early-weaned piglets. Twelve pairs of normal birth weight and intrauterine growth-retarded (IUGR) piglets (seven days old) were randomly assigned to receive adequate nutrient intake or restricted nutrient intake for 21 days. Serum and muscle samples were collected for further analysis. The results indicated that muscle weight, cross-sectional areas, and muscular glycogen were lower (p < 0.05) in both IUGR and restricted fed piglets. Nutrient restriction decreased the contents of RNA, the RNA to DNA ratio, and the percentages of myosin heavy chain (MyHC) IIx (p < 0.05), whereas increased the activity of β-hydroxy-acyl-CoA-dehydrogenase (HAD), the ratio of HAD to citrate synthase, as well as the percentages of MyHC I (p < 0.05). In addition, nutrient restriction significantly decreased muscular glycogen, mRNA levels of fatty acid transport protein 1, cationic amino acid transporter 1, and glucose transporter 4 in IUGR piglets compared with the other groups (p < 0.05). The results of the present study showed that IUGR impaired skeletal muscle growth and disturbed the hormone and mRNA expression of genes related to energy metabolism, which led to a more severe energy deficit when receiving postnatal nutritional restriction. Postnatal nutritional restriction resulted in delayed myofiber maturation of the piglets, which may be associated with the transformation of MyHC isoform and the change of metabolic status.

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Effect of protein nutrition and amino acid balance early in life on porcine skeletal muscle development

10.31274/rtd-180813-3788 ◽

1976 ◽

Author(s):

Maynard Gordon Hogberg

Keyword(s):

Skeletal Muscle ◽

Amino Acid ◽

Muscle Development ◽

Skeletal Muscle Development ◽

Protein Nutrition ◽

Amino Acid Balance

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Effect of protein nutrition and amino acid balance early in life on porcine skeletal muscle development

10.31274/rtd-180817-3788 ◽

1976 ◽

Author(s):

Maynard Gordon Hogberg

Keyword(s):

Skeletal Muscle ◽

Amino Acid ◽

Muscle Development ◽

Skeletal Muscle Development ◽

Protein Nutrition ◽

Amino Acid Balance

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Key Genes Regulating Skeletal Muscle Development and Growth in Farm Animals

Animals ◽

10.3390/ani11030835 ◽

2021 ◽

Vol 11 (3) ◽

pp. 835

Author(s):

Mohammadreza Mohammadabadi ◽

Farhad Bordbar ◽

Just Jensen ◽

Min Du ◽

Wei Guo

Keyword(s):

Skeletal Muscle ◽

Candidate Genes ◽

Meat Quality ◽

Muscle Development ◽

Muscle Growth ◽

Farm Animals ◽

Meat Production ◽

Skeletal Muscle Development ◽

Extrinsic Factors ◽

Myogenic Regulatory Factor

Farm-animal species play crucial roles in satisfying demands for meat on a global scale, and they are genetically being developed to enhance the efficiency of meat production. In particular, one of the important breeders’ aims is to increase skeletal muscle growth in farm animals. The enhancement of muscle development and growth is crucial to meet consumers’ demands regarding meat quality. Fetal skeletal muscle development involves myogenesis (with myoblast proliferation, differentiation, and fusion), fibrogenesis, and adipogenesis. Typically, myogenesis is regulated by a convoluted network of intrinsic and extrinsic factors monitored by myogenic regulatory factor genes in two or three phases, as well as genes that code for kinases. Marker-assisted selection relies on candidate genes related positively or negatively to muscle development and can be a strong supplement to classical selection strategies in farm animals. This comprehensive review covers important (candidate) genes that regulate muscle development and growth in farm animals (cattle, sheep, chicken, and pig). The identification of these genes is an important step toward the goal of increasing meat yields and improves meat quality.

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