Analysis of Antioxidant Activity, Chlorogenic Acid, and Rutin Content of Camellia sinensis Infusions Using Response Surface Methodology Optimization

The amount of spent coffee grounds (SCGs) created, represents an environmental challenge worldwide. In this context, the aim of the present study was to exploit the potential of SCGs as a source of bioactive compounds that can be utilized in high value-added products. Thus, a cost-effective and environmentally friendly extraction technique was developed to ensure extracts with high total phenolic content and antioxidant activity, as well as significant amounts of caffeine and chlorogenic acid. Response surface methodology was implemented to evaluate the effects of the main extraction parameters (i.e., time, temperature, and ethanol-to-water ratio) and their interactions on the defined responses. The ethanol ratio was found to be the most significant variable. Then, a set of optimum values was determined (i.e., 7 min, 75 °C, and ethanol:water ratio 5:95), where the predicted values for responses were found to be 5.65% for the yield (Y1), 152.68 mg gallic acid equivalents per L for total phenolic content (Y2), 0.797 μmol Trolox equivalent per mL for the antioxidant activity (Y3), 30.5 ppm for caffeine concentration (Y4), and 17.4 ppm for chlorogenic acid concentration (Y5). Furthermore, the corresponding high experimental values from the validation experiment fitted well to these predictions, clearly clarifying the high potential of SCG extracts for use in high value-added applications.

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EXTRACTION OPTIMIZATION AND ANTIOXIDANT ACTIVITY OF PHENOLIC COMPOUNDS FROM AVOCADO PEEL

Journal of Medicine and Pharmacy ◽

10.34071/mp.2019.3.7 ◽

2019 ◽

pp. 49-59

Author(s):

Nu Linh Giang Ton ◽

Thi Hoai Nguyen ◽

Quoc Hung Vo

Keyword(s):

Antioxidant Activity ◽

Response Surface Methodology ◽

Antioxidant Capacity ◽

Response Surface ◽

Total Antioxidant Capacity ◽

Total Phenolic Content ◽

Phenolic Content ◽

Quadratic Model ◽

Total Phenolic ◽

Total Antioxidant

Avocado peel has been considered as a potential source of natural antioxidants in which phenolics are among the most important compounds. Therefore, this study aims to optimize the extraction process of phenolics using response surface methodology and evaluate the corresponding antioxidant activity. From the quadratic model, the optimal condition was determined including the ethanol concentration 54.55% (v/v), the solvent/solute ratio 71.82/1 (mL/g), temperature 53.03 oC and extraction time 99.09 min. The total phenolic content and the total antioxidant capacity at this condition with minor modifications were 26,74 ± 0,04 (mg GAE/g DW) and 188.06 ± 1.41 (mg AAE/g DW), respectively. The significant correlation between total phenolic content and total antioxidant capacity was also confirmed. Key words: response surface methodology, central composite rotatable design, total phenolic content, total antioxidant capacity, avocado peel

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Optimization of enzymatic hydrolysis of immature citrus (Citrus unshiu Marcov.) for flavonoid content and antioxidant activity using a response surface methodology

Food Science and Biotechnology ◽

10.1007/s10068-021-00897-w ◽

2021 ◽

Author(s):

Kwang-Seup Shin ◽

Jeung-Hee Lee

Keyword(s):

Antioxidant Activity ◽

Response Surface Methodology ◽

Enzymatic Hydrolysis ◽

Response Surface ◽

Citrus Unshiu ◽

Flavonoid Content ◽

Hydrolysis Of

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Optimizing the Extraction of Catechin from Peanut Red Skin Using Response Surface Methodology and its Antioxidant Activity

IERI Procedia ◽

10.1016/j.ieri.2013.11.109 ◽

2013 ◽

Vol 5 ◽

pp. 312-320 ◽

Cited By ~ 6

Author(s):

Haiyue Zhang ◽

Man Liu ◽

Shengting Han ◽

Ying Wei

Keyword(s):

Antioxidant Activity ◽

Response Surface Methodology ◽

Response Surface

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Optimization of Synthesis of Seleno-Sargassum fusiforme(Harv.) Setch. Polysaccharide by Response Surface Methodology, Its Characterization, and Antioxidant Activity

Journal of Chemistry ◽

10.1155/2013/493524 ◽

2013 ◽

Vol 2013 ◽

pp. 1-9 ◽

Cited By ~ 4

Author(s):

Yu-Bin Ji ◽

Fang Dong ◽

Miao Yu ◽

Long Qin ◽

Dan Liu

Keyword(s):

Antioxidant Activity ◽

Response Surface Methodology ◽

Reaction Time ◽

Response Surface ◽

Reaction Temperature ◽

Sargassum Fusiforme ◽

Synthesis Conditions ◽

Optimum Reaction ◽

Series Of Experiments

The response surface methodology was employed to optimize the synthesis conditions of seleno-Sargassum fusiforme(Harv.) Setch. polysaccharide. Three independent variables (reaction time, reaction temperature, and ratio of Na2SeO3to SFPSI) were tested. Furthermore, the characterization and antioxidant activity of Se-SFPSIin vivowere investigated. The result showed that the actual experimental Se content of Se-SFPSI was 3.352 mg/g at the optimum reaction conditions of reaction time 8 h, reaction temperature 71°C, and ratio of Na2SeO3to SFPSIB 1.0 g/g. A series of experiments showed that the characterization of Se-SFPSIB was significantly different from that of SFPSIB. Additionally, antioxidant activity assay indicated that the Se-SFPSIB could increase catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) activity of mice bearing tumor S180in blood, heart, and liver while decreasing malondialdehyde (MDA) levels. It can be concluded that selenylation is a feasible approach to obtain seleno-polysaccharide which was utilized as highly biological medicine or functional food.

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