scholarly journals Introduction of Arabidopsis’s heat shock factor HsfA1d mitigates adverse effects of heat stress on potato (Solanum tuberosum L.) plant

2020 ◽  
Vol 25 (1) ◽  
pp. 57-63
Author(s):  
Zamarud Shah ◽  
Safdar Hussain Shah ◽  
Gul Shad Ali ◽  
Iqbal Munir ◽  
Raham Sher Khan ◽  
...  
Keyword(s):  
Heat Stress ◽  
Solanum Tuberosum ◽  
Heat Shock ◽  
Adverse Effects ◽  
Solanum Tuberosum L ◽  
Heat Shock Factor

scholarly journals Expression of small heat shock proteins and heat tolerance in potato (Solanum tuberosum L.)

2012 ◽  
Vol 64 (1) ◽  
pp. 135-144 ◽  
Author(s):  
Jelena Savic ◽  
Ivana Dragicevic ◽  
D. Pantelic ◽  
Jasmina Oljaca ◽  
Ivana Momcilovic
Keyword(s):  
Heat Stress ◽  
Solanum Tuberosum ◽  
Heat Shock ◽  
Heat Tolerance ◽  
Solanum Tuberosum L ◽  
Immunoblot Analysis ◽  
Control Treatment ◽  
Ex Vitro ◽  
Heat Tolerant

We have examined the correlation between heat tolerance and small heat shock protein (sHSP) expression under heat stress conditions in potato (Solanum tuberosum L.). The relative heat tolerance of nine potato cultivars grown under greenhouse conditions was determined using the electrolyte leakage assay (ELA), a standard quantitative assay for heat tolerance. Three cultivars differing in heat tolerance were selected and designated as heat-tolerant (?Laura?), moderately sensitive (?Liseta?) and heat-sensitive (?Agria?) genotypes. The expression of cytosolic HSP18 and chloroplast HSP21 was analyzed at the protein level in the leaves of selected cultivars, both ex vitro- and in vitro-grown, after heat stress or control treatment. Immunoblot analysis revealed heat-induced HSP18 and HSP21 expression in all examined genotypes. A similar pattern of examined sHSP expression was observed ex vitro and in vitro: heat-tolerant ?Laura? accumulated higher levels of both HSP18 and HSP21 compared to heat-sensitive ?Liseta? and ?Agria?. Our results indicate that ELA combined with immunoblot analysis of sHSP accumulation under HS conditions, might be considered as a reliable procedure in screening potato genotypes for heat tolerance. To our knowledge, this is the first study where sHSP expression between ex vitro- and in vitro-grown potato plants was compared.

scholarly journals Seleksi Kultivar Kentang (Solanum tuberosum L.) Berdasarkan Penampilan Karakter Agronomis di Dataran Medium Kabupaten Garut

2018 ◽  
Vol 3 (1) ◽  
pp. 1
Author(s):  
Jajang Supriatna ◽  
Resti Fajarfika ◽  
Asep Bagja ◽  
Juniarti P Sahat
Keyword(s):  
Heat Stress ◽  
Solanum Tuberosum ◽  
Solanum Tuberosum L

Penanaman kentang pada kawasan dataran medium akan dihadapkan dengan masalah cekaman lingkungan terutama suhu tinggi (heat stress) yang menyebabkan adanya perubahan penampilan sebagai respons terhadap cekaman. Adanya perbedaan respons diantara kultivar menunjukkan tingkat adaptasi yang beragam. Sebuah penelitian yang bertujuan untuk menyeleksi kultivar kentang berdasarkan penampilan karakter agronomis di dataran medium Kabupaten Garut sebagai langkah awal seleksi dan evaluasi materi genetik potensial dalam pengembangan kultivar kentang adaptif dataran medium Kabupaten Garut. Percobaan telah dilaksanakan pada bulan Juli sampai Oktober 2018 di Tarogong Kaler Kabupaten Garut dengan ketinggian 732 meter di atas permukaan laut (m dpl). Percobaan disusun dalam Rancangan Acak Kelompok dengan menggunakan 15 kultivar kentang sebagai perlakuan dan diulang sebanyak tiga kali. Kultivar-kultivar yang digunakan terdiri dari 14 kultivar uji yaitu 1) Andina, 2) Sangkuriang, 3) Cipanas, 4) AR-08, 5) Amabile, 6) Atlantik Malang, 7) Dayang Sumbi, 8) GM-05, 9) Merbabu-17, 10) Vernei, 11) Tedzo MZ, 12) Median, 13) Erika, dan 14) Granola, serta 1 kultivar pembanding yaitu Olimpus. Hasil penelitian menunjukkan terseleksi sejumlah kultivar kentang yang memiliki penampilan agronomis lebih baik dibandingkan dengan kultivar pembanding diantaranya 2 kultivar berdasarkan tinggi tanaman, 2 kultivar berdasarkan luas daun, 5 kultivar berdasarkan jumlah umbi per tanaman dan 10 kultivar berdasarkan diameter umbi. Kultivar GM-05 dan Vernei memiliki kemampuan adaptasi yang baik pada kawasan dataran medium Kabupaten Garut berdasarkan karakter tinggi tanaman, luas daun dan jumlah umbi per tanaman. Kata Kunci: Solanum tuberosum L.; karakter agronomis; heat stress; dataran medium

In-season heat stress compromises postharvest quality and low-temperature sweetening resistance in potato (Solanum tuberosum L.)

Planta ◽  
2014 ◽  
Vol 239 (6) ◽  
pp. 1243-1263 ◽  
Author(s):  
Daniel H. Zommick ◽  
Lisa O. Knowles ◽  
Mark J. Pavek ◽  
N. Richard Knowles
Keyword(s):  
Heat Stress ◽  
Solanum Tuberosum ◽  
Low Temperature ◽  
Solanum Tuberosum L ◽  
Postharvest Quality ◽  
Low Temperature Sweetening

Measurement of heat injury in plant tissue by using electrical impedance analysis

1993 ◽  
Vol 71 (12) ◽  
pp. 1605-1611 ◽  
Author(s):  
M. I. N. Zhang ◽  
J. H. M. Willison ◽  
M. A. Cox ◽  
S. A. Hall
Keyword(s):  
Heat Stress ◽  
Solanum Tuberosum ◽  
Electrical Impedance ◽  
Solanum Tuberosum L ◽  
Impedance Analysis ◽  
Membrane Capacitance ◽  
Temperature Changes ◽  
Heat Injury ◽  
Membrane Thermostability ◽  
Extracellular Resistance

Electrical impedance spectra (100 Hz to 800 kHz) were measured in pieces of potato (Solanum tuberosum L.) tuber tissue that had been heat stressed. Analysis of the impedance data based on equivalent circuits showed that tissue injury was revealed as decreases in membrane capacitance and extracellular resistance. By using a single piece of tissue, membrane thermostability (in relation to heat stress temperature or time of heat stress) was measured. Nonstressful temperature changes produced changes in tissue parameters that were fully reversible when the direction of temperature change was reversed. Stressful temperature changes produced irreversible changes in parameters. During heat injury, decrease in extracellular resistance always preceded a decrease in tonoplast capacitance. It is suggested that two stages may be involved in heat injury to membranes: functional injury leading to electrolyte leakage to extracellular space, and structural damage leading to membrane disintegration. It is concluded that electrical impedance analysis is useful in plant heat stress physiology. Key words: potato (Solanum tuberosum L.) tuber, electrical impedance, membrane capacitance, heat injury, membrane thermostability.

Glutamine's protection against cellular injury is dependent on heat shock factor-1

2006 ◽  
Vol 290 (6) ◽  
pp. C1625-C1632 ◽  
Author(s):  
Angela L. Morrison ◽  
Martin Dinges ◽  
Kristen D. Singleton ◽  
Kelli Odoms ◽  
Hector R. Wong ◽  
...  
Keyword(s):  
Heat Stress ◽  
Heat Shock ◽  
Heat Shock Factor ◽  
Tetrazolium Salt ◽  
Heat Shock Factor 1 ◽  
Dependent Manner ◽  
Hsp 70 ◽  
Stress Injury ◽  
Cellular Protection ◽  
Dose Dependent

Glutamine (GLN) has been shown to protect cells, tissues, and whole organisms from stress and injury. Enhanced expression of heat shock protein (HSP) has been hypothesized to be responsible for this protection. To date, there are no clear mechanistic data confirming this relationship. This study tested the hypothesis that GLN-mediated activation of the HSP pathway via heat shock factor-1 (HSF-1) is responsible for cellular protection. Wild-type HSF-1 (HSF-1+/+) and knockout (HSF-1−/−) mouse fibroblasts were used in all experiments. Cells were treated with GLN concentrations ranging from 0 to 16 mM and exposed to heat stress injury in a concurrent treatment model. Cell viability was assayed with phenazine methosulfate plus tetrazolium salt, HSP-70, HSP-25, and nuclear HSF-1 expression via Western blot analysis, and HSF-1/heat shock element (HSE) binding via EMSA. GLN significantly attenuated heat-stress induced cell death in HSF-1+/+ cells in a dose-dependent manner; however, the survival benefit of GLN was lost in HSF-1−/− cells. GLN led to a dose-dependent increase in HSP-70 and HSP-25 expression after heat stress. No inducible HSP expression was observed in HSF-1−/− cells. GLN increased unphosphorylated HSF-1 in the nucleus before heat stress. This was accompanied by a GLN-mediated increase in HSF-1/HSE binding and nuclear content of phosphorylated HSF-1 after heat stress. This is the first demonstration that GLN-mediated cellular protection after heat-stress injury is related to HSF-1 expression and cellular capacity to activate an HSP response. Furthermore, the mechanism of GLN-mediated protection against injury appears to involve an increase in nuclear HSF-1 content before stress and increased HSF-1 promoter binding and phosphorylation.

Human heat shock factor 1 is predominantly a nuclear protein before and after heat stress

1999 ◽  
Vol 112 (16) ◽  
pp. 2765-2774 ◽  
Author(s):  
P.A. Mercier ◽  
N.A. Winegarden ◽  
J.T. Westwood
Keyword(s):  
Heat Stress ◽  
Heat Shock ◽  
Nuclear Protein ◽  
Heat Shock Factor ◽  
Nuclear Fraction ◽  
Heat Shock Factor 1 ◽  
Heat Shock Genes ◽  
Before And After ◽  
Biochemical Fractionation ◽  
Multistep Process

The induction of the heat shock genes in eukaryotes by heat and other forms of stress is mediated by a transcription factor known as heat shock factor 1 (HSF1). HSF1 is present in unstressed metazoan cells as a monomer with low affinity for DNA, and upon exposure to stress it is converted to an ‘active’ homotrimer that binds the promoters of heat shock genes with high affinity and induces their transcription. The conversion of HSF1 to its active form is hypothesized to be a multistep process involving physical changes in the HSF1 molecule and the possible translocation of HSF1 from the cytoplasm to the nucleus. While all studies to date have found active HSF1 to be a nuclear protein, there have been conflicting reports on whether the inactive form of HSF is predominantly a cytoplasmic or nuclear protein. In this study, we have made antibodies against human HSF1 and have reexamined its localization in unstressed and heat-shocked human HeLa and A549 cells, and in green monkey Vero cells. Biochemical fractionation of heat-shocked HeLa cells followed by western blot analysis showed that HSF1 was mostly found in the nuclear fraction. In extracts made from unshocked cells, HSF1 was predominantly found in the cytoplasmic fraction using one fractionation procedure, but was distributed approximately equally between the cytoplasmic and nuclear fractions when a different procedure was used. Immunofluorescence microscopy revealed that HSF1 was predominantly a nuclear protein in both heat shocked and unstressed cells. Quantification of HSF1 staining showed that approximately 80% of HSF1 was present in the nucleus both before and after heat stress. These results suggest that HSF1 is predominantly a nuclear protein prior to being exposed to stress, but has low affinity for the nucleus and is easily extracted using most biochemical fractionation procedures. These results also imply that HSF1 translocation is probably not part of the multistep process in HSF1 activation for many cell types.

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