Green tea polyphenols function as prooxidants to inhibit Pseudomonas aeruginosa and induce the expression of oxidative stress-related genes

Green tea, prepared from the leaves ofCamellia sinensisL., is a beverage that is popular worldwide. Polyphenols in green tea have been receiving much attention as potential compounds for the maintenance of human health due to their varied biological activity and low toxicity. In particular, the contribution of antioxidant activity to the prevention of diseases caused by oxidative stress has been focused upon. Therefore, in this study, we investigated the effects of (−)-epigallocatechin 3-O-gallate and (−)-epigallocatechin 3-O-gallate, which account for a large fraction of the components of green tea polyphenol, on oxidative stress-related renal disease. Our observations suggest that green tea polyphenols have a beneficial effect on pathological states related to oxidative stress of the kidney.

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Green Tea Polyphenols Function as Prooxidants To Activate Oxidative-Stress-Responsive Transcription Factors in Yeasts

Applied and Environmental Microbiology ◽

10.1128/aem.01963-06 ◽

2006 ◽

Vol 73 (2) ◽

pp. 572-580 ◽

Cited By ~ 60

Author(s):

Kazuhiro Maeta ◽

Wataru Nomura ◽

Yoshifumi Takatsume ◽

Shingo Izawa ◽

Yoshiharu Inoue

Keyword(s):

Oxidative Stress ◽

Transcription Factors ◽

Green Tea ◽

Nuclear Localization ◽

Disulfide Bonds ◽

Epigallocatechin Gallate ◽

Tea Polyphenols ◽

Green Tea Polyphenols ◽

Alkaline Conditions ◽

Tea Extract

ABSTRACT Epigallocatechin gallate (EGCG) is the most abundant polyphenolic flavonoid in green tea. Catechin and its derivatives, including EGCG, are widely believed to function as antioxidants. Here we demonstrate that both EGCG and green tea extract (GTE) cause oxidative stress-related responses in the budding yeast Saccharomyces cerevisiae and the fission yeast Schizosaccharomyces pombe under weak alkaline conditions in terms of the activation of oxidative-stress-responsive transcription factors. GTE as well as EGCG induced the nuclear localization of Yap1 in S. cerevisiae, which was repressed by the addition of catalase but not by the addition of superoxide dismutase. The same phenomena were observed for the nucleocytoplasmic localization of Msn2 in S. cerevisiae and Pap1, a Yap1 homologue, in S. pombe. The formation of intramolecular disulfide bonds has been proposed to be crucial for the H2O2-induced nuclear localization of Yap1, and we verified the importance of cysteine residues of Yap1 in response to EGCG and GTE. Additionally, we show that EGCG and GTE produce H2O2 in a weak alkaline medium. Finally, we conclude that tea polyphenols are able to act as prooxidants to cause a response to oxidative stress in yeasts under certain conditions.

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Green Tea Polyphenols for the Protection of Internal Organs—Focus on Renal Damage Caused by Oxidative Stress

Green Tea Polyphenols ◽

10.1201/b14878-10 ◽

2013 ◽

pp. 177-204

Author(s):

Takako Yokozawa ◽

Jeong Noh ◽

Chan Park ◽

Jong Park

Keyword(s):

Oxidative Stress ◽

Green Tea ◽

Renal Damage ◽

Tea Polyphenols ◽

Green Tea Polyphenols ◽

Internal Organs

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Green tea polyphenols improve isoflurane-induced cognitive impairment via modulating oxidative stress

The Journal of Nutritional Biochemistry ◽

10.1016/j.jnutbio.2019.07.004 ◽

2019 ◽

Vol 73 ◽

pp. 108213 ◽

Cited By ~ 1

Author(s):

Yuting Song ◽

Xiuxiu Li ◽

Xiangdan Gong ◽

Xin Zhao ◽

Zhengliang Ma ◽

...

Keyword(s):

Oxidative Stress ◽

Cognitive Impairment ◽

Green Tea ◽

Tea Polyphenols ◽

Green Tea Polyphenols

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Green Tea Polyphenols Ameliorate the Early Renal Damage Induced by a High-Fat Diet via Ketogenesis/SIRT3 Pathway

Oxidative Medicine and Cellular Longevity ◽

10.1155/2017/9032792 ◽

2017 ◽

Vol 2017 ◽

pp. 1-14 ◽

Cited By ~ 7

Author(s):

Weijie Yi ◽

Xiao Xie ◽

Miying Du ◽

Yongjun Bu ◽

Nannan Wu ◽

...

Keyword(s):

Oxidative Stress ◽

Green Tea ◽

High Fat Diet ◽

Tea Polyphenols ◽

Green Tea Polyphenols ◽

Activity Levels ◽

High Fat ◽

Sirt3 Expression ◽

Renal Expression ◽

Expression And Activity

Scope. Several reports in the literature have suggested the renoprotective effects of ketone bodies and green tea polyphenols (GTPs). Our previous study found that GTP consumption could elevate the renal expression of the ketogenic rate-limiting enzyme, which was decreased by a high-fat diet (HFD) in rats. Here, we investigated whether ketogenesis can mediate renoprotection by GTPs against an HFD. Methods and Results. Wistar rats were fed a standard or HFD with or without GTPs for 18 weeks. The renal oxidative stress level, kidney function, renal expression, and activity levels of mitochondrial 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase 2 (HMGCS2) and sirtuin 3(SIRT3) were detected. The increased renal oxidative stress and the loss of renal function induced by the HFD were ameliorated by GTPs. Renal ketogenesis and SIRT3 expression and activity levels, which were reduced by the HFD, were restored by GTPs. In vitro, HEK293 cells were transfected with the eukaryotic expression plasmid pcDNA HMGCS2. GTP treatment could upregulate HMGCS2 and SIRT3 expression. Although SIRT3 expression was not affected by HMGCS2 transfection, the 4-hydroxy-2-nonenal (4-HNE) level and the acetyl-MnSOD (K122)/MnSOD ratio were reduced in HMGCS2-transfected cells in the context of H2O2. Conclusion. The ketogenesis/SIRT3 pathway mediates the renoprotection of GTPs against the oxidative stress induced by an HFD.

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